O96438 (O96438_CRIFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Tryparedoxin EMBL AAC72299.1

Submitted name:
Tryparedoxin I EMBL AAD20445.1

Gene names

Name:	TryX EMBL AAC72299.1
Synonyms:	txnI EMBL AAD20445.1

Organism

Crithidia fasciculata EMBL AAC72299.1

Taxonomic identifier

5656 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Crithidia

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Technical term	3D-structure PDB 1O8W PDB 1OKD PDB 1O7U PDB 1O8X PDB 1O85 PDB 1EZK PDB 1EWX PDB 1QK8
Gene Ontology (GO)
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O96438 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C9663DE8FD803D43	FASTA	146	16,481
	10 20 30 40 50 60 MSGLDKYLPG IEKLRRGDGE VEVKSLAGKL VFFYFSASWC PPCRGFTPQL IEFYDKFHES 70 80 90 100 110 120 KNFEVVFCTW DEEEDGFAGY FAKMPWLAVP FAQSEAVQKL SKHFNVESIP TLIGVDADSG 130 140 DVVTTRARAT LVKDPEGEQF PWKDAP

References

[1]	"The high resolution crystal structure of recombinant Crithidia fasciculata tryparedoxin-I." Alphey M.S., Leonard G.A., Gourley D.G., Tetaud E., Fairlamb A.H., Hunter W.N. J. Biol. Chem. 274:25613-25622(1999) [PubMed: 10464297] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS).
[2]	"NMR studies of the interaction of tryparedoxin with redox-inactive substrate homologues." Krumme D., Budde H., Hecht H.J., Menge U., Ohlenschlager O., Ross A., Wissing J., Wray V., Flohe L. Biochemistry 42:14720-14728(2003) [PubMed: 14674746] [Abstract] Cited for: STRUCTURE BY NMR.
[3]	"Structures of tryparedoxins revealing interaction with trypanothione." Hofmann B., Budde H., Bruns K., Guerrero S.A., Kalisz H.M., Menge U., Montemartini M., Nogoceke E., Steinert P., Wissing J.B., Flohe L., Hecht H.J. Biol. Chem. 382:459-471(2001) [PubMed: 11347894] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-146.
[4]	"Cloning, expression and reconstitution of the trypanothione-dependent peroxidase system of Crithidia fasciculata." Tetaud E., Fairlamb A.H. Mol. Biochem. Parasitol. 96:111-123(1998) [PubMed: 9851611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Sequence, heterologous expression and functional characterization of a novel tryparedoxin from Crithidia fasciculata." Montemartini M., Kalisz H.M., Kiess M., Nogoceke E., Singh M., Steinert P., Flohe L. Biol. Chem. 379:1137-1142(1998) [PubMed: 9792447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: HS6 EMBL AAD20445.1.
[6]	"Sequence, heterologous expression and functional characterization of tryparedoxin1 from Crithidia fasciculata." Guerrero S.A., Flohe L., Kalisz H.M., Montemartini M., Nogoceke E., Hecht H.J., Steinert P., Singh M. Eur. J. Biochem. 259:789-794(1999) [PubMed: 10092865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: HS6 EMBL AAD20445.1.
[7]	"Tryparedoxins from Crithidia fasciculata and Trypanosoma brucei: photoreduction of the redox disulfide using synchrotron radiation and evidence for a conformational switch implicated in function." Alphey M.S., Gabrielsen M., Micossi E., Leonard G.A., McSweeney S.M., Ravelli R.B., Tetaud E., Fairlamb A.H., Bond C.S., Hunter W.N. J. Biol. Chem. 278:25919-25925(2003) [PubMed: 12707277] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS).
[8]	Guerrero S., Flohe L., Kalisz H.M., Montemartini M., Nogoceke E., Hecht H.-J., Steinert P., Singh M. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: HS6 EMBL AAD20445.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF055913 Genomic DNA. Translation: AAC72299.1.
AF084456 Genomic DNA. Translation: AAD20445.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EWX	X-ray	1.70	A	2-146	[»]
1EZK	X-ray	1.90	A	2-146	[»]
1O7U	X-ray	1.50	A	1-146	[»]
1O85	X-ray	1.50	A	1-146	[»]
1O8W	X-ray	1.70	A	1-146	[»]
1O8X	X-ray	1.30	A	1-146	[»]
1OKD	NMR	-	A	1-146	[»]
1QK8	X-ray	1.40	A	1-146	[»]

ProteinModelPortal

O96438.

SMR

O96438. Positions 2-146.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF08534. Redoxin. 1 hit.
[Graphical view]

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

O96438_CRIFA

Accession

Primary (citable) accession number: O96438

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1999
Last sequence update:	May 1, 1999
Last modified:	October 19, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)