Reviewed, UniProtKB/Swiss-Prot O96024 (B3GT4_HUMAN)

Names and origin

Protein names

Recommended name:
    Beta-1,3-galactosyltransferase 4
      Short name=Beta-1,3-GalTase 4
      Short name=Beta3Gal-T4
      Short name=b3Gal-T4
      Short name=GalT4
    EC=2.4.1.62
Alternative name(s):
    GAL-T2
    Ganglioside galactosyltransferase
    UDP-galactose:beta-N-acetyl-galactosamine-beta-1,3-galactosyltransferase

Gene names

Name:	B3GALT4
Synonyms:	GALT4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in GM1/GD1B/GA1 ganglioside biosynthesis. Ref.1
Catalytic activity	UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-(1->4)-beta-D-glucosyl-N-acylsphingosine = UDP + D-galactosyl-(1->3)-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Highly expressed in heart, skeletal muscle and pancreas and, to a lesser extent, in brain, placenta, kidney, liver and lung. Ref.1 Ref.2
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Ref.1 Traceable author statement. Source: ProtInc ganglioside galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Beta-1,3-galactosyltransferase 4		PRO_0000219160
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 19	11	Signal-anchor for type II membrane protein Potential
	Topological domain	20 – 378	359	Lumenal Potential
Amino acid modifications
	Glycosylation	149	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O96024-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4E55DFC72AE96213	FASTA	378	41,537
	10 20 30 40 50 60 MQLRLFRRLL LAALLLVIVW TLFGPSGLGE ELLSLSLASL LPAPASPGPP LALPRLLIPN 70 80 90 100 110 120 QEACSGPGAP PFLLILVCTA PENLNQRNAI RASWGGLREA RGLRVQTLFL LGEPNAQHPV 130 140 150 160 170 180 WGSQGSDLAS ESAAQGDILQ AAFQDSYRNL TLKTLSGLNW AEKHCPMARY VLKTDDDVYV 190 200 210 220 230 240 NVPELVSELV LRGGRWGQWE RSTEPQREAE QEGGQVLHSE EVPLLYLGRV HWRVNPSRTP 250 260 270 280 290 300 GGRHRVSEEQ WPHTWGPFPP YASGTGYVLS ASAVQLILKV ASRAPLLPLE DVFVGVSARR 310 320 330 340 350 360 GGLAPTQCVK LAGATHYPLD RCCYGKFLLT SHRLDPWKMQ EAWKLVGGSD GERTAPFCSW 370 FQGVLGILRC RAIAWLQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of human beta3-galactosyltransferases. Characterization of four members of a UDP-galactose:beta-N-acetyl-glucosamine/beta-nacetyl-galactosamine beta-1,3-galactosyltransferase family." Amado M., Almeida R., Carneiro F., Levery S.B., Holmes E.H., Nomoto M., Hollingsworth M.A., Hassan H., Schwientek T., Nielsen P.A., Bennett E.P., Clausen H. J. Biol. Chem. 273:12770-12778(1998) [PubMed: 9582303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"The beta 1,3-galactosyltransferase-4 (b3Gal-T4) gene is located in the centromeric segment of the human MHC class II region." Shiina T., Kikkawa E., Iwasaki H., Kaneko M., Narimatsu H., Sasaki K., Bahram S., Inoko H. Immunogenetics 51:75-78(2000) [PubMed: 10663566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal brain.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta-1,3-galactosyltransferase 4

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y15061 mRNA. Translation: CAA75345.1. AL031228 Genomic DNA. Translation: CAA20230.1. AB026730 mRNA. Translation: BAA88988.1. BC032574 mRNA. Translation: AAH32574.1.
IPI	IPI00304588.
RefSeq	NP_003773.1.
UniGene	Hs.534375
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O96024. 1 interaction.
STRING	O96024.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
PTM databases
PhosphoSite	O96024.
Proteomic databases
PRIDE	O96024.
Genome annotation databases
Ensembl	ENST00000383209; ENSP00000372696; ENSG00000206285; Homo sapiens. [Genome view] ENST00000415322; ENSP00000394876; ENSG00000226936; Homo sapiens. [Genome view] ENST00000419471; ENSP00000398660; ENSG00000235155; Homo sapiens. [Genome view] ENST00000430971; ENSP00000414880; ENSG00000236802; Homo sapiens. [Genome view] ENST00000451237; ENSP00000390784; ENSG00000235863; Homo sapiens. [Genome view]
GeneID	8705.
KEGG	hsa:8705.
UCSC	uc003odr.1. human.
Organism-specific databases
CTD	8705.
GeneCards	GC06P033352.
H-InvDB	HIX0005773. HIX0058137. HIX0078463.
HGNC	HGNC:919. B3GALT4.
MIM	603095. gene.
PharmGKB	PA25212.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O96024.
HOVERGEN	O96024.
OMA	THCVKLA
OrthoDB	EOG9M0HMR
Enzyme and pathway databases
BRENDA	2.4.1.62. 247.
Gene expression databases
CleanEx	HS_B3GALT4.
Genevestigator	O96024.
GermOnline	ENSG00000204222. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	32641.
SOURCE	Search...

Entry information

Entry name

B3GT4_HUMAN

Accession

Primary (citable) accession number: O96024

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	May 1, 1999
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.