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O96020 (CCNE2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 16, 2012. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G1/S-specific cyclin-E2
Gene names
Name:CCNE2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the control of the cell cycle at the late G1 and early S phase.

Subunit structure

Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.

Subcellular location

Nucleus Ref.3.

Tissue specificity

According to Ref.1, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to Ref.2: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to Ref.3 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

Induction

Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively.

Post-translational modification

Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway By similarity.

Sequence similarities

Belongs to the cyclin family. Cyclin E subfamily.

Ontologies

Keywords
   Biological processCell cycle
Cell division
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   Molecular functionCyclin
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processG1/S transition of mitotic cell cycle

Traceable author statement. Source: Reactome

cell cycle checkpoint

Traceable author statement Ref.1. Source: ProtInc

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of cyclin-dependent protein kinase activity

Traceable author statement Ref.3. Source: ProtInc

   Cellular componentcytosol

Traceable author statement. Source: Reactome

nucleoplasm

Traceable author statement. Source: Reactome

   Molecular functionprotein kinase binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDK2P249415EBI-375033,EBI-375096
CDKN1AP389362EBI-375033,EBI-375077
CDKN1BP465272EBI-375033,EBI-519280

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: O96020-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: O96020-2)

Also known as: SV;

The sequence of this isoform differs from the canonical sequence as follows:
     167-211: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404G1/S-specific cyclin-E2
PRO_0000080461

Amino acid modifications

Modified residue191Phosphothreonine Ref.7
Modified residue211Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8
Modified residue3831Phosphoserine Ref.5
Modified residue3921Phosphothreonine Ref.5

Natural variations

Alternative sequence167 – 21145Missing in isoform Short.
VSP_001256
Natural variant3871N → S. Ref.4
Corresponds to variant rs28399585 [ dbSNP | Ensembl ].
VAR_021347

Experimental info

Mutagenesis3921T → A: Increase of steady state level. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: D7DC9BEEF3FD62EC

FASTA40446,757
        10         20         30         40         50         60 
MSRRSSRLQA KQQPQPSQTE SPQEAQIIQA KKRKTTQDVK KRREEVTKKH QYEIRNCWPP 

        70         80         90        100        110        120 
VLSGGISPCI IIETPHKEIG TSDFSRFTNY RFKNLFINPS PLPDLSWGCS KEVWLNMLKK 

       130        140        150        160        170        180 
ESRYVHDKHF EVLHSDLEPQ MRSILLDWLL EVCEVYTLHR ETFYLAQDFF DRFMLTQKDI 

       190        200        210        220        230        240 
NKNMLQLIGI TSLFIASKLE EIYAPKLQEF AYVTDGACSE EDILRMELII LKALKWELCP 

       250        260        270        280        290        300 
VTIISWLNLF LQVDALKDAP KVLLPQYSQE TFIQIAQLLD LCILAIDSLE FQYRILTAAA 

       310        320        330        340        350        360 
LCHFTSIEVV KKASGLEWDS ISECVDWMVP FVNVVKSTSP VKLKTFKKIP MEDRHNIQTH 

       370        380        390        400 
TNYLAMLEEV NYINTFRKGG QLSPVCNGGI MTPPKSTEKP PGKH

« Hide

Isoform Short (SV) [UniParc].

Checksum: 2A774B409828EC47
Show »

FASTA35941,465

References

« Hide 'large scale' references
[1]"Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers."
Gudas J.M., Payton M., Thukral S., Chen E., Bass M., Robinson M.O., Coats S.
Mol. Cell. Biol. 19:612-622(1999) [PubMed: 9858585] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Tissue: Fetal lung.
[2]"Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle."
Lauper N., Beck A.R.P., Cariou S., Richman L., Hofmann K., Reith W., Slingerland J.M., Amati B.
Oncogene 17:2637-2643(1998) [PubMed: 9840927] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: B-cell.
[3]"Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins."
Zariwala M., Liu J., Xiong Y.
Oncogene 17:2787-2798(1998) [PubMed: 9840943] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF THR-392.
Tissue: Keratinocyte.
[4]NIEHS SNPs program
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-387.
[5]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-383 AND THR-392, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[7]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19 AND SER-21, MASS SPECTROMETRY.
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF106690 mRNA. Translation: AAD08816.1.
AF112857 mRNA. Translation: AAD08819.1.
AF091433 mRNA. Translation: AAC80528.1.
AF102778 mRNA. Translation: AAC78145.1.
AY850195 Genomic DNA. Translation: AAV97813.1.
IPIIPI00014085.
IPI00219502.
RefSeqNP_477097.1. NM_057749.2.
UniGeneHs.521693.

3D structure databases

ProteinModelPortalO96020.
SMRO96020. Positions 100-369.
ModBaseSearch...

Protein-protein interaction databases

IntActO96020. 7 interactions.
MINTMINT-1353336.
STRINGO96020.

PTM databases

PhosphoSiteO96020.

Proteomic databases

PRIDEO96020.

Protocols and materials databases

DNASU9134.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308108; ENSP00000309181; ENSG00000175305.
GeneID9134.
KEGGhsa:9134.
UCSCuc003yhc.3. human.

Organism-specific databases

CTD9134.
GeneCardsGC08M095892.
H-InvDBHIX0007656.
HGNCHGNC:1590. CCNE2.
HPACAB007825.
CAB019374.
MIM603775. gene.
neXtProtNX_O96020.
PharmGKBPA26155.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5024.
GeneTreeENSGT00650000093050.
HOGENOMHOG000231743.
HOVERGENHBG050834.
InParanoidO96020.
KOK06626.
OMAFIQIAQL.
OrthoDBEOG4Z36F4.
PhylomeDBO96020.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.

Gene expression databases

ArrayExpressO96020.
BgeeO96020.
CleanExHS_CCNE2.
GenevestigatorO96020.
GermOnlineENSG00000175305. Homo sapiens.

Family and domain databases

Gene3DG3DSA:1.10.472.10. Cyclin_related. 2 hits.
InterProIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E.
IPR004367. Cyclin_C.
IPR006671. Cyclin_N.
[Graphical view]
PfamPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMSSF47954. Cyclin_like. 1 hit.
PROSITEPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio34249.
SOURCESearch...

Entry information

Entry nameCCNE2_HUMAN
AccessionPrimary (citable) accession number: O96020
Secondary accession number(s): O95439
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1999
Last modified: May 16, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents