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O96020

- CCNE2_HUMAN

UniProt

O96020 - CCNE2_HUMAN

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Protein
G1/S-specific cyclin-E2
Gene
CCNE2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the control of the cell cycle at the late G1 and early S phase.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase regulator activity Source: Ensembl
  2. protein binding Source: IntAct

GO - Biological processi

  1. DNA replication initiation Source: Ensembl
  2. G1/S transition of mitotic cell cycle Source: Reactome
  3. cell cycle checkpoint Source: ProtInc
  4. cell division Source: UniProtKB-KW
  5. mitotic cell cycle Source: Reactome
  6. regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiREACT_111214. G0 and Early G1.
REACT_1625. p53-Dependent G1 DNA Damage Response.
REACT_169185. DNA Damage/Telomere Stress Induced Senescence.
REACT_308. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
SignaLinkiO96020.

Names & Taxonomyi

Protein namesi
Recommended name:
G1/S-specific cyclin-E2
Gene namesi
Name:CCNE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:1590. CCNE2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi392 – 3921T → A: Increase of steady state level. 1 Publication

Organism-specific databases

PharmGKBiPA26155.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404G1/S-specific cyclin-E2
PRO_0000080461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211Phosphoserine2 Publications
Modified residuei383 – 3831Phosphoserine1 Publication
Modified residuei392 – 3921Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation by CDK2 triggers its release from CDK2 and degradation via the ubiquitin proteasome pathway By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO96020.
PaxDbiO96020.
PRIDEiO96020.

PTM databases

PhosphoSiteiO96020.

Expressioni

Tissue specificityi

According to 1 Publication, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to 1 Publication: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to 1 Publication highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

Inductioni

Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively.

Gene expression databases

ArrayExpressiO96020.
BgeeiO96020.
CleanExiHS_CCNE2.
GenevestigatoriO96020.

Organism-specific databases

HPAiCAB007825.
CAB019374.

Interactioni

Subunit structurei

Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
CDK2P249417EBI-375033,EBI-375096
CDKN1AP389362EBI-375033,EBI-375077
CDKN1BP465272EBI-375033,EBI-519280

Protein-protein interaction databases

BioGridi114582. 13 interactions.
DIPiDIP-31733N.
IntActiO96020. 8 interactions.
MINTiMINT-1353336.
STRINGi9606.ENSP00000309181.

Structurei

3D structure databases

ProteinModelPortaliO96020.
SMRiO96020. Positions 100-369.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5024.
HOGENOMiHOG000231743.
HOVERGENiHBG050834.
InParanoidiO96020.
KOiK06626.
OMAiYSQEKFI.
OrthoDBiEOG7HB595.
PhylomeDBiO96020.
TreeFamiTF101005.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR028858. Cyclin_E.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10177:SF70. PTHR10177:SF70. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: O96020-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSRRSSRLQA KQQPQPSQTE SPQEAQIIQA KKRKTTQDVK KRREEVTKKH    50
QYEIRNCWPP VLSGGISPCI IIETPHKEIG TSDFSRFTNY RFKNLFINPS 100
PLPDLSWGCS KEVWLNMLKK ESRYVHDKHF EVLHSDLEPQ MRSILLDWLL 150
EVCEVYTLHR ETFYLAQDFF DRFMLTQKDI NKNMLQLIGI TSLFIASKLE 200
EIYAPKLQEF AYVTDGACSE EDILRMELII LKALKWELCP VTIISWLNLF 250
LQVDALKDAP KVLLPQYSQE TFIQIAQLLD LCILAIDSLE FQYRILTAAA 300
LCHFTSIEVV KKASGLEWDS ISECVDWMVP FVNVVKSTSP VKLKTFKKIP 350
MEDRHNIQTH TNYLAMLEEV NYINTFRKGG QLSPVCNGGI MTPPKSTEKP 400
PGKH 404
Length:404
Mass (Da):46,757
Last modified:May 1, 1999 - v1
Checksum:iD7DC9BEEF3FD62EC
GO
Isoform Short (identifier: O96020-2) [UniParc]FASTAAdd to Basket

Also known as: SV

The sequence of this isoform differs from the canonical sequence as follows:
     167-211: Missing.

Show »
Length:359
Mass (Da):41,465
Checksum:i2A774B409828EC47
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti387 – 3871N → S.1 Publication
Corresponds to variant rs28399585 [ dbSNP | Ensembl ].
VAR_021347

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei167 – 21145Missing in isoform Short.
VSP_001256Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF106690 mRNA. Translation: AAD08816.1.
AF112857 mRNA. Translation: AAD08819.1.
AF091433 mRNA. Translation: AAC80528.1.
AF102778 mRNA. Translation: AAC78145.1.
AY850195 Genomic DNA. Translation: AAV97813.1.
CCDSiCCDS6264.1. [O96020-1]
RefSeqiNP_477097.1. NM_057749.2. [O96020-1]
UniGeneiHs.521693.

Genome annotation databases

EnsembliENST00000308108; ENSP00000309181; ENSG00000175305. [O96020-1]
ENST00000520509; ENSP00000429089; ENSG00000175305. [O96020-1]
GeneIDi9134.
KEGGihsa:9134.
UCSCiuc003yhc.3. human. [O96020-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF106690 mRNA. Translation: AAD08816.1 .
AF112857 mRNA. Translation: AAD08819.1 .
AF091433 mRNA. Translation: AAC80528.1 .
AF102778 mRNA. Translation: AAC78145.1 .
AY850195 Genomic DNA. Translation: AAV97813.1 .
CCDSi CCDS6264.1. [O96020-1 ]
RefSeqi NP_477097.1. NM_057749.2. [O96020-1 ]
UniGenei Hs.521693.

3D structure databases

ProteinModelPortali O96020.
SMRi O96020. Positions 100-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114582. 13 interactions.
DIPi DIP-31733N.
IntActi O96020. 8 interactions.
MINTi MINT-1353336.
STRINGi 9606.ENSP00000309181.

Chemistry

BindingDBi O96020.
ChEMBLi CHEMBL2111444.

PTM databases

PhosphoSitei O96020.

Proteomic databases

MaxQBi O96020.
PaxDbi O96020.
PRIDEi O96020.

Protocols and materials databases

DNASUi 9134.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308108 ; ENSP00000309181 ; ENSG00000175305 . [O96020-1 ]
ENST00000520509 ; ENSP00000429089 ; ENSG00000175305 . [O96020-1 ]
GeneIDi 9134.
KEGGi hsa:9134.
UCSCi uc003yhc.3. human. [O96020-1 ]

Organism-specific databases

CTDi 9134.
GeneCardsi GC08M095892.
HGNCi HGNC:1590. CCNE2.
HPAi CAB007825.
CAB019374.
MIMi 603775. gene.
neXtProti NX_O96020.
PharmGKBi PA26155.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5024.
HOGENOMi HOG000231743.
HOVERGENi HBG050834.
InParanoidi O96020.
KOi K06626.
OMAi YSQEKFI.
OrthoDBi EOG7HB595.
PhylomeDBi O96020.
TreeFami TF101005.

Enzyme and pathway databases

Reactomei REACT_111214. G0 and Early G1.
REACT_1625. p53-Dependent G1 DNA Damage Response.
REACT_169185. DNA Damage/Telomere Stress Induced Senescence.
REACT_308. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
REACT_9003. SCF(Skp2)-mediated degradation of p27/p21.
SignaLinki O96020.

Miscellaneous databases

ChiTaRSi CCNE2. human.
GenomeRNAii 9134.
NextBioi 34249.
PROi O96020.
SOURCEi Search...

Gene expression databases

ArrayExpressi O96020.
Bgeei O96020.
CleanExi HS_CCNE2.
Genevestigatori O96020.

Family and domain databases

Gene3Di 1.10.472.10. 2 hits.
InterProi IPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR028858. Cyclin_E.
IPR006671. Cyclin_N.
[Graphical view ]
PANTHERi PTHR10177:SF70. PTHR10177:SF70. 1 hit.
Pfami PF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTi SM00385. CYCLIN. 1 hit.
[Graphical view ]
SUPFAMi SSF47954. SSF47954. 2 hits.
PROSITEi PS00292. CYCLINS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers."
    Gudas J.M., Payton M., Thukral S., Chen E., Bass M., Robinson M.O., Coats S.
    Mol. Cell. Biol. 19:612-622(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Tissue: Fetal lung.
  2. "Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle."
    Lauper N., Beck A.R.P., Cariou S., Richman L., Hofmann K., Reith W., Slingerland J.M., Amati B.
    Oncogene 17:2637-2643(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: B-cell.
  3. "Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins."
    Zariwala M., Liu J., Xiong Y.
    Oncogene 17:2787-2798(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF THR-392.
    Tissue: Keratinocyte.
  4. NIEHS SNPs program
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-387.
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383 AND THR-392, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiCCNE2_HUMAN
AccessioniPrimary (citable) accession number: O96020
Secondary accession number(s): O95439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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