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Protein

Actin-like protein 6A

Gene

ACTL6A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: ProtInc
  • transcription coactivator activity Source: BHF-UCL

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • chromatin remodeling Source: BHF-UCL
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • histone H2A acetylation Source: UniProtKB
  • histone H4 acetylation Source: UniProtKB
  • neural retina development Source: BHF-UCL
  • positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
  • protein deubiquitination Source: Reactome
  • regulation of growth Source: UniProtKB-KW
  • regulation of mitophagy Source: ParkinsonsUK-UCL
  • regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • signal transduction Source: ProtInc
  • spinal cord development Source: Ensembl
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chromatin regulator
Biological processDNA damage, DNA recombination, DNA repair, Growth regulation, Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214847. HATs acetylate histones.
R-HSA-3214858. RMTs methylate histone arginines.
R-HSA-5689603. UCH proteinases.
R-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-8939243. RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known.
SignaLinkiO96019.
SIGNORiO96019.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-like protein 6A
Alternative name(s):
53 kDa BRG1-associated factor A
Actin-related protein Baf53a
ArpNbeta
BRG1-associated factor 53A
Short name:
BAF53A
INO80 complex subunit K
Gene namesi
Name:ACTL6A
Synonyms:BAF53, BAF53A, INO80K
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000136518.16.
HGNCiHGNC:24124. ACTL6A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi86.
OpenTargetsiENSG00000136518.
PharmGKBiPA134914203.

Polymorphism and mutation databases

BioMutaiACTL6A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000891332 – 429Actin-like protein 6AAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei86PhosphoserineCombined sources1
Modified residuei233PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO96019.
MaxQBiO96019.
PaxDbiO96019.
PeptideAtlasiO96019.
PRIDEiO96019.
TopDownProteomicsiO96019-1. [O96019-1]

PTM databases

iPTMnetiO96019.
PhosphoSitePlusiO96019.

Miscellaneous databases

PMAP-CutDBiO96019.

Expressioni

Gene expression databases

BgeeiENSG00000136518.
CleanExiHS_ACTL6A.
ExpressionAtlasiO96019. baseline and differential.
GenevisibleiO96019. HS.

Organism-specific databases

HPAiCAB012188.
HPA018671.

Interactioni

Subunit structurei

Component of numerous complexes with chromatin remodeling and histone acetyltransferase activity. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM/BAF190B, and TRRAP/PAF400, and which may also include a HAT activity related to, but distinct from, that of KAT5. ACTL6A interacts with SMARCA4/BRG1/BAF190A. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the DBINO domain of INO80. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Interacts with PHF10/BAF45A (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
YY1P254906EBI-355018,EBI-765538

Protein-protein interaction databases

BioGridi106601. 118 interactors.
CORUMiO96019.
DIPiDIP-27565N.
IntActiO96019. 62 interactors.
MINTiMINT-1136373.
STRINGi9606.ENSP00000397552.

Structurei

3D structure databases

ProteinModelPortaliO96019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0679. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00840000129754.
HOGENOMiHOG000233340.
HOVERGENiHBG003771.
InParanoidiO96019.
KOiK11340.
OMAiDGIVEDW.
OrthoDBiEOG091G0A1S.
PhylomeDBiO96019.
TreeFamiTF312863.

Family and domain databases

InterProiView protein in InterPro
IPR029912. ACL6A.
IPR004000. Actin.
IPR004001. Actin_CS.
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF279. PTHR11937:SF279. 1 hit.
PfamiView protein in Pfam
PF00022. Actin. 1 hit.
PRINTSiPR00190. ACTIN.
SMARTiView protein in SMART
SM00268. ACTIN. 1 hit.
PROSITEiView protein in PROSITE
PS00432. ACTINS_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O96019-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGGVYGGDE VGALVFDIGS YTVRAGYAGE DCPKVDFPTA IGMVVERDDG
60 70 80 90 100
STLMEIDGDK GKQGGPTYYI DTNALRVPRE NMEAISPLKN GMVEDWDSFQ
110 120 130 140 150
AILDHTYKMH VKSEASLHPV LMSEAPWNTR AKREKLTELM FEHYNIPAFF
160 170 180 190 200
LCKTAVLTAF ANGRSTGLIL DSGATHTTAI PVHDGYVLQQ GIVKSPLAGD
210 220 230 240 250
FITMQCRELF QEMNIELVPP YMIASKEAVR EGSPANWKRK EKLPQVTRSW
260 270 280 290 300
HNYMCNCVIQ DFQASVLQVS DSTYDEQVAA QMPTVHYEFP NGYNCDFGAE
310 320 330 340 350
RLKIPEGLFD PSNVKGLSGN TMLGVSHVVT TSVGMCDIDI RPGLYGSVIV
360 370 380 390 400
AGGNTLIQSF TDRLNRELSQ KTPPSMRLKL IANNTTVERR FSSWIGGSIL
410 420
ASLGTFQQMW ISKQEYEEGG KQCVERKCP
Length:429
Mass (Da):47,461
Last modified:May 1, 1999 - v1
Checksum:iECD92D29BD1854E0
GO
Isoform 2 (identifier: O96019-2) [UniParc]FASTAAdd to basket
Also known as: HArpNbeta-s

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:387
Mass (Da):43,236
Checksum:i5FF2B5B41C15C03A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92M → T in CAB66543 (PubMed:11230166).Curated1
Sequence conflicti210F → L in CAB66543 (PubMed:11230166).Curated1
Sequence conflicti254M → T in CAB66543 (PubMed:11230166).Curated1
Sequence conflicti320N → K in CAB66543 (PubMed:11230166).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0001501 – 42Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041474 mRNA. Translation: AAC94991.1.
AB015907 mRNA. Translation: BAA74577.1.
AB060168 mRNA. Translation: BAB87844.1.
AB061315 mRNA. Translation: BAB87848.1.
AL136608 mRNA. Translation: CAB66543.1.
AK021756 mRNA. Translation: BAG51043.1.
CH471052 Genomic DNA. Translation: EAW78401.1.
CH471052 Genomic DNA. Translation: EAW78397.1.
CH471052 Genomic DNA. Translation: EAW78399.1.
BC000949 mRNA. Translation: AAH00949.1.
BC001391 mRNA. Translation: AAH01391.1.
BC036371 mRNA. Translation: AAH36371.1.
CCDSiCCDS3231.1. [O96019-1]
CCDS43174.1. [O96019-2]
RefSeqiNP_004292.1. NM_004301.4. [O96019-1]
NP_817126.1. NM_177989.3. [O96019-2]
NP_829888.1. NM_178042.3. [O96019-2]
UniGeneiHs.435326.

Genome annotation databases

EnsembliENST00000392662; ENSP00000376430; ENSG00000136518. [O96019-2]
ENST00000429709; ENSP00000397552; ENSG00000136518. [O96019-1]
ENST00000450518; ENSP00000394014; ENSG00000136518. [O96019-2]
GeneIDi86.
KEGGihsa:86.
UCSCiuc003fjw.4. human. [O96019-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACL6A_HUMAN
AccessioniPrimary (citable) accession number: O96019
Secondary accession number(s): B3KMN1
, D3DNR9, Q8TAE5, Q9BVS8, Q9H0W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 1999
Last modified: September 27, 2017
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families