Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O96018 (APBA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amyloid beta A4 precursor protein-binding family A member 3
Alternative name(s):
Adapter protein X11gamma
Neuron-specific X11L2 protein
Neuronal Munc18-1-interacting protein 3
Short name=Mint-3
Gene names
Name:APBA3
Synonyms:MINT3, X11L2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN. Ref.8

Subunit structure

Binds to the cytoplasmic domain of amyloid protein (APP) in vivo. Interacts with HIF1AN (via N-terminus). Ref.8

Subcellular location

Cytoplasmperinuclear region Ref.8.

Tissue specificity

Expressed in all tissues examined with lower levels in brain and testis.

Domain

Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

Sequence similarities

Contains 2 PDZ (DHR) domains.

Contains 1 PID domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Azi2Q9QYP62EBI-6115839,EBI-6115874From a different organism.
TANKQ928442EBI-6115839,EBI-356349
TBKBP1A7MCY62EBI-6115839,EBI-359969

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Amyloid beta A4 precursor protein-binding family A member 3
PRO_0000064620

Regions

Domain217 – 381165PID
Domain394 – 48087PDZ 1
Domain485 – 56076PDZ 2
Compositional bias165 – 1717Poly-Ser

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10
Modified residue3721Phosphoserine Ref.7

Natural variations

Natural variant1541W → L.
Corresponds to variant rs35932323 [ dbSNP | Ensembl ].
VAR_050666
Natural variant2761K → T.
Corresponds to variant rs3746119 [ dbSNP | Ensembl ].
VAR_020134
Natural variant3761C → R. Ref.7
Corresponds to variant rs8102086 [ dbSNP | Ensembl ].
VAR_047952
Natural variant5271I → F.
Corresponds to variant rs1045236 [ dbSNP | Ensembl ].
VAR_011822

Experimental info

Sequence conflict5051I → IVRPRPLAPGWGGRAALSTA PEQPPPLSRAPLFLPQ in AAC17979. Ref.5

Secondary structure

................................... 575
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O96018 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 3B910CC74C5F3840

FASTA57561,454
        10         20         30         40         50         60 
MDFPTISRSP SGPPAMDLEG PRDILVPSED LTPDSQWDPM PGGPGSLSRM ELDESSLQEL 

        70         80         90        100        110        120 
VQQFEALPGD LVGPSPGGAP CPLHIATGHG LASQEIADAH GLLSAEAGRD DLLGLLHCEE 

       130        140        150        160        170        180 
CPPSQTGPEE PLEPAPRLLQ PPEDPDEDSD SPEWVEGASA EQEGSRSSSS SPEPWLETVP 

       190        200        210        220        230        240 
LVTPEEPPAG AQSPETLASY PAPQEVPGPC DHEDLLDGVI FGARYLGSTQ LVSERNPPTS 

       250        260        270        280        290        300 
TRMAQAREAM DRVKAPDGET QPMTEVDLFV STKRIKVLTA DSQEAMMDHA LHTISYTADI 

       310        320        330        340        350        360 
GCVLVLMARR RLARRPAPQD HGRRLYKMLC HVFYAEDAQL IAQAIGQAFA AAYSQFLRES 

       370        380        390        400        410        420 
GIDPSQVGVH PSPGACHLHN GDLDHFSNSD NCREVHLEKR RGEGLGVALV ESGWGSLLPT 

       430        440        450        460        470        480 
AVIANLLHGG PAERSGALSI GDRLTAINGT SLVGLPLAAC QAAVRETKSQ TSVTLSIVHC 

       490        500        510        520        530        540 
PPVTTAIIHR PHAREQLGFC VEDGIICSLL RGGIAERGGI RVGHRIIEIN GQSVVATPHA 

       550        560        570 
RIIELLTEAY GEVHIKTMPA ATYRLLTGQE QPVYL 

« Hide

References

« Hide 'large scale' references
[1]"X11L2, a new member of X11 protein family interacts with Alzheimer's beta-amyloid precursor protein."
Tanahashi H., Tabira T.
Biochem. Biophys. Res. Commun. 255:663-667(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[4]"Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein."
Tanahashi H., Tabira T.
NeuroReport 10:2575-2578(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-575.
[5]"Mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2."
Okamoto M., Suedhof T.C.
Eur. J. Cell Biol. 77:161-165(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 472-575.
[6]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, VARIANT [LARGE SCALE ANALYSIS] ARG-376, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1."
Sakamoto T., Seiki M.
J. Biol. Chem. 284:30350-30359(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HIF1AN, SUBCELLULAR LOCATION.
[9]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Solution structure of the PDZ domain of amyloid beta A4 precursor protein-binding family A member 3."
RIKEN structural genomics initiative (RSGI)
Submitted (APR-2008) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 387-569.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB021638 mRNA. Translation: BAA74430.1.
AC005954 Genomic DNA. Translation: AAC72275.1.
BC086306 mRNA. Translation: AAH86306.1.
AB023431 Genomic DNA. Translation: BAA83094.1.
AF029110 mRNA. Translation: AAC17979.1.
CCDSCCDS12110.1.
PIRJG0181.
RefSeqNP_004877.1. NM_004886.3.
UniGeneHs.25527.
Hs.465607.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YT7NMR-A390-483[»]
2YT8NMR-A483-569[»]
ProteinModelPortalO96018.
SMRO96018. Positions 213-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114920. 7 interactions.
IntActO96018. 4 interactions.
MINTMINT-1207318.
STRING9606.ENSP00000315136.

PTM databases

PhosphoSiteO96018.

Proteomic databases

MaxQBO96018.
PaxDbO96018.
PRIDEO96018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000316757; ENSP00000315136; ENSG00000011132.
GeneID9546.
KEGGhsa:9546.
UCSCuc002lyo.1. human.

Organism-specific databases

CTD9546.
GeneCardsGC19M003750.
HGNCHGNC:580. APBA3.
HPAHPA045577.
MIM604262. gene.
neXtProtNX_O96018.
PharmGKBPA24872.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG283022.
HOGENOMHOG000033981.
HOVERGENHBG050523.
InParanoidO96018.
OMAALHTISY.
OrthoDBEOG78PV8H.
PhylomeDBO96018.
TreeFamTF315245.

Gene expression databases

ArrayExpressO96018.
BgeeO96018.
CleanExHS_APBA3.
GenevestigatorO96018.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProIPR001478. PDZ.
IPR011993. PH_like_dom.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 2 hits.
PROSITEPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAPBA3. human.
EvolutionaryTraceO96018.
GeneWikiAPBA3.
GenomeRNAi9546.
NextBio35799.
PROO96018.
SOURCESearch...

Entry information

Entry nameAPBA3_HUMAN
AccessionPrimary (citable) accession number: O96018
Secondary accession number(s): O60483, Q9UPZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM