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Protein

Napsin-A

Gene

NAPSA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in processing of pneumocyte surfactant precursors.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961PROSITE-ProRule annotation
Active sitei283 – 2831PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB
  2. endopeptidase activity Source: UniProtKB
  3. peptidase activity Source: UniProtKB

GO - Biological processi

  1. membrane protein proteolysis Source: UniProtKB
  2. proteolysis Source: UniProtKB
  3. surfactant homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.046.

Names & Taxonomyi

Protein namesi
Recommended name:
Napsin-A (EC:3.4.23.-)
Alternative name(s):
Aspartyl protease 4
Short name:
ASP4
Short name:
Asp 4
Napsin-1
TA01/TA02
Gene namesi
Name:NAPSA
Synonyms:NAP1, NAPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:13395. NAPSA.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. alveolar lamellar body Source: UniProtKB
  2. extracellular space Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProtKB
  4. lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134891814.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Propeptidei26 – 6338Activation peptidePRO_0000025996Add
BLAST
Chaini64 – 420357Napsin-APRO_0000025997Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi109 ↔ 116By similarity
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi274 ↔ 278By similarity
Disulfide bondi317 ↔ 354By similarity
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiO96009.
PRIDEiO96009.

PTM databases

PhosphoSiteiO96009.

Expressioni

Tissue specificityi

Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.

Gene expression databases

BgeeiO96009.
CleanExiHS_NAPSA.
ExpressionAtlasiO96009. baseline and differential.
GenevestigatoriO96009.

Organism-specific databases

HPAiCAB009591.
HPA045280.
HPA047236.
HPA047744.

Interactioni

Protein-protein interaction databases

BioGridi114861. 6 interactions.
IntActiO96009. 1 interaction.
MINTiMINT-3318113.
STRINGi9606.ENSP00000253719.

Structurei

3D structure databases

ProteinModelPortaliO96009.
SMRiO96009. Positions 68-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248684.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO96009.
KOiK08565.
OMAiVIQTTRN.
OrthoDBiEOG7HQN88.
PhylomeDBiO96009.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O96009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP
60 70 80 90 100
AELPKLGAPS PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS
110 120 130 140 150
NLWVPSRRCH FFSVPCWLHH RFDPKASSSF QANGTKFAIQ YGTGRVDGIL
160 170 180 190 200
SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR
210 220 230 240 250
PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP AHYIPPLTFV
260 270 280 290 300
PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH
310 320 330 340 350
AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG
360 370 380 390 400
VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR
410 420
ARTRGADLGW GETAQAQFPG
Length:420
Mass (Da):45,387
Last modified:May 1, 1999 - v1
Checksum:i018B86AE5BD0C865
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti40 – 401I → T.1 Publication
Corresponds to variant rs676314 [ dbSNP | Ensembl ].
VAR_051510
Natural varianti310 – 3101A → T.
Corresponds to variant rs11670727 [ dbSNP | Ensembl ].
VAR_024586

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090386 mRNA. Translation: AAD04917.1.
AF200345 mRNA. Translation: AAF17081.1.
AF098484 mRNA. Translation: AAD13215.1.
BC017842 mRNA. Translation: AAH17842.1.
CCDSiCCDS12794.1.
RefSeqiNP_004842.1. NM_004851.1.
UniGeneiHs.512843.

Genome annotation databases

EnsembliENST00000253719; ENSP00000253719; ENSG00000131400.
GeneIDi9476.
KEGGihsa:9476.
UCSCiuc002prx.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090386 mRNA. Translation: AAD04917.1.
AF200345 mRNA. Translation: AAF17081.1.
AF098484 mRNA. Translation: AAD13215.1.
BC017842 mRNA. Translation: AAH17842.1.
CCDSiCCDS12794.1.
RefSeqiNP_004842.1. NM_004851.1.
UniGeneiHs.512843.

3D structure databases

ProteinModelPortaliO96009.
SMRiO96009. Positions 68-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114861. 6 interactions.
IntActiO96009. 1 interaction.
MINTiMINT-3318113.
STRINGi9606.ENSP00000253719.

Protein family/group databases

MEROPSiA01.046.

PTM databases

PhosphoSiteiO96009.

Proteomic databases

PaxDbiO96009.
PRIDEiO96009.

Protocols and materials databases

DNASUi9476.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253719; ENSP00000253719; ENSG00000131400.
GeneIDi9476.
KEGGihsa:9476.
UCSCiuc002prx.3. human.

Organism-specific databases

CTDi9476.
GeneCardsiGC19M050861.
H-InvDBHIX0039966.
HGNCiHGNC:13395. NAPSA.
HPAiCAB009591.
HPA045280.
HPA047236.
HPA047744.
MIMi605631. gene.
neXtProtiNX_O96009.
PharmGKBiPA134891814.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG248684.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO96009.
KOiK08565.
OMAiVIQTTRN.
OrthoDBiEOG7HQN88.
PhylomeDBiO96009.
TreeFamiTF314990.

Miscellaneous databases

GeneWikiiNAPSA.
GenomeRNAii9476.
NextBioi35512.
PROiO96009.
SOURCEiSearch...

Gene expression databases

BgeeiO96009.
CleanExiHS_NAPSA.
ExpressionAtlasiO96009. baseline and differential.
GenevestigatoriO96009.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Napsins: new human aspartic proteinases. Distinction between two closely related genes."
    Tatnell P.J., Powell D.J., Hill J., Smith T.S., Tew D.G., Kay J.
    FEBS Lett. 441:43-48(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney and Lung.
  2. "Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas."
    Chuman Y., Bergman A.-C., Ueno T., Saito S., Sakaguchi K., Alaiya A.A., Franzen B., Bergman T., Arnott D., Auer G., Appella E., Joernvall H., Linder S.
    FEBS Lett. 462:129-134(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fetal lung.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1."
    Koelsch G., Wu S., Henthorn J., Tang J., Lin X.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-40.
    Tissue: Lung.

Entry informationi

Entry nameiNAPSA_HUMAN
AccessioniPrimary (citable) accession number: O96009
Secondary accession number(s): Q8WWD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.