O96009 (NAPSA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Napsin-A
EC=3.4.23.-

Alternative name(s):
Aspartyl protease 4
Short name=ASP4
Short name=Asp 4
Napsin-1
TA01/TA02

Gene names

Name:	NAPSA
Synonyms:	NAP1, NAPA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in processing of pneumocyte surfactant precursors.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	membrane protein proteolysis Inferred from direct assay PubMed 14766755. Source: UniProtKB surfactant homeostasis Inferred from direct assay PubMed 18216060. Source: UniProtKB
Cellular_component	alveolar lamellar body Inferred from direct assay PubMed 18216060. Source: UniProtKB extracellular space Inferred from direct assay PubMed 18216060. Source: UniProtKB lysosome Inferred from direct assay PubMed 14766755. Source: UniProtKB
Molecular_function	aspartic-type endopeptidase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 63	38	Activation peptide		PRO_0000025996
	Chain	64 – 420	357	Napsin-A		PRO_0000025997
Sites
	Active site	96	1	By similarity
	Active site	283	1	By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 116		By similarity
	Disulfide bond	274 ↔ 278		By similarity
	Disulfide bond	317 ↔ 354		By similarity
Natural variations
	Natural variant	40	1	I → T. Ref.5 Corresponds to variant rs676314 [ dbSNP | Ensembl ].		VAR_051510
	Natural variant	310	1	A → T. Corresponds to variant rs11670727 [ dbSNP | Ensembl ].		VAR_024586

Sequences

	Sequence		Length	Mass (Da)
	O96009 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 018B86AE5BD0C865	FASTA	420	45,387
	10 20 30 40 50 60 MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP AELPKLGAPS 70 80 90 100 110 120 PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS NLWVPSRRCH FFSVPCWLHH 130 140 150 160 170 180 RFDPKASSSF QANGTKFAIQ YGTGRVDGIL SEDKLTIGGI KGASVIFGEA LWEPSLVFAF 190 200 210 220 230 240 AHFDGILGLG FPILSVEGVR PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP 250 260 270 280 290 300 AHYIPPLTFV PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH 310 320 330 340 350 360 AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG VRLCLSGFQA 370 380 390 400 410 420 LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR ARTRGADLGW GETAQAQFPG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Napsins: new human aspartic proteinases. Distinction between two closely related genes." Tatnell P.J., Powell D.J., Hill J., Smith T.S., Tew D.G., Kay J. FEBS Lett. 441:43-48(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney and Lung.
[2]	"Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas." Chuman Y., Bergman A.-C., Ueno T., Saito S., Sakaguchi K., Alaiya A.A., Franzen B., Bergman T., Arnott D., Auer G., Appella E., Joernvall H., Linder S. FEBS Lett. 462:129-134(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Fetal lung.
[3]	"Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity." Yan R., Bienkowski M.J., Shuck M.E., Miao H., Tory M.C., Pauley A.M., Brashier J.R., Stratman N.C., Mathews W.R., Buhl A.E., Carter D.B., Tomasselli A.G., Parodi L.A., Heinrikson R.L., Gurney M.E. Nature 402:533-537(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1." Koelsch G., Wu S., Henthorn J., Tang J., Lin X. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-40. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF090386 mRNA. Translation: AAD04917.1. AF200345 mRNA. Translation: AAF17081.1. AF098484 mRNA. Translation: AAD13215.1. BC017842 mRNA. Translation: AAH17842.1.
IPI	IPI00014055.
RefSeq	NP_004842.1. NM_004851.1.
UniGene	Hs.512843.
3D structure databases
ProteinModelPortal	O96009.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3318113.
STRING	9606.ENSP00000253719.
Protein family/group databases
MEROPS	A01.046.
PTM databases
PhosphoSite	O96009.
Proteomic databases
PaxDb	O96009.
PRIDE	O96009.
Protocols and materials databases
DNASU	9476.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253719; ENSP00000253719; ENSG00000131400.
GeneID	9476.
KEGG	hsa:9476.
UCSC	uc002prx.3. human.
Organism-specific databases
CTD	9476.
GeneCards	GC19M050861.
H-InvDB	HIX0039966.
HGNC	HGNC:13395. NAPSA.
HPA	CAB009591.
MIM	605631. gene.
neXtProt	NX_O96009.
PharmGKB	PA134891814.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG248684.
HOGENOM	HOG000197681.
HOVERGEN	HBG000482.
InParanoid	O96009.
KO	K08565.
OMA	GLTLCAQ.
OrthoDB	EOG40GCR5.
PhylomeDB	O96009.
Gene expression databases
Bgee	O96009.
CleanEx	HS_NAPSA.
Genevestigator	O96009.
GermOnline	ENSG00000131400. Homo sapiens.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9476.
NextBio	35512.
SOURCE	Search...

Entry information

Entry name

NAPSA_HUMAN

Accession

Primary (citable) accession number: O96009
Secondary accession number(s): Q8WWD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.