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O96009 (NAPSA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Napsin-A

EC=3.4.23.-
Alternative name(s):
Aspartyl protease 4
Short name=ASP4
Short name=Asp 4
Napsin-1
TA01/TA02
Gene names
Name:NAPSA
Synonyms:NAP1, NAPA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in processing of pneumocyte surfactant precursors.

Subcellular location

Secreted Potential.

Tissue specificity

Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.

Sequence similarities

Belongs to the peptidase A1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 6338Activation peptide
PRO_0000025996
Chain64 – 420357Napsin-A
PRO_0000025997

Sites

Active site961 By similarity
Active site2831 By similarity

Amino acid modifications

Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation3361N-linked (GlcNAc...) Potential
Disulfide bond109 ↔ 116 By similarity
Disulfide bond274 ↔ 278 By similarity
Disulfide bond317 ↔ 354 By similarity

Natural variations

Natural variant401I → T. Ref.5
Corresponds to variant rs676314 [ dbSNP | Ensembl ].
VAR_051510
Natural variant3101A → T.
Corresponds to variant rs11670727 [ dbSNP | Ensembl ].
VAR_024586

Sequences

Sequence LengthMass (Da)Tools
O96009 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 018B86AE5BD0C865

FASTA42045,387
        10         20         30         40         50         60 
MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP AELPKLGAPS 

        70         80         90        100        110        120 
PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS NLWVPSRRCH FFSVPCWLHH 

       130        140        150        160        170        180 
RFDPKASSSF QANGTKFAIQ YGTGRVDGIL SEDKLTIGGI KGASVIFGEA LWEPSLVFAF 

       190        200        210        220        230        240 
AHFDGILGLG FPILSVEGVR PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP 

       250        260        270        280        290        300 
AHYIPPLTFV PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH 

       310        320        330        340        350        360 
AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG VRLCLSGFQA 

       370        380        390        400        410        420 
LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR ARTRGADLGW GETAQAQFPG 

« Hide

References

« Hide 'large scale' references
[1]"Napsins: new human aspartic proteinases. Distinction between two closely related genes."
Tatnell P.J., Powell D.J., Hill J., Smith T.S., Tew D.G., Kay J.
FEBS Lett. 441:43-48(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney and Lung.
[2]"Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas."
Chuman Y., Bergman A.-C., Ueno T., Saito S., Sakaguchi K., Alaiya A.A., Franzen B., Bergman T., Arnott D., Auer G., Appella E., Joernvall H., Linder S.
FEBS Lett. 462:129-134(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Fetal lung.
[3]"Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity."
Yan R., Bienkowski M.J., Shuck M.E., Miao H., Tory M.C., Pauley A.M., Brashier J.R., Stratman N.C., Mathews W.R., Buhl A.E., Carter D.B., Tomasselli A.G., Parodi L.A., Heinrikson R.L., Gurney M.E.
Nature 402:533-537(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1."
Koelsch G., Wu S., Henthorn J., Tang J., Lin X.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-40.
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF090386 mRNA. Translation: AAD04917.1.
AF200345 mRNA. Translation: AAF17081.1.
AF098484 mRNA. Translation: AAD13215.1.
BC017842 mRNA. Translation: AAH17842.1.
CCDSCCDS12794.1.
RefSeqNP_004842.1. NM_004851.1.
UniGeneHs.512843.

3D structure databases

ProteinModelPortalO96009.
SMRO96009. Positions 68-401.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114861. 2 interactions.
IntActO96009. 1 interaction.
MINTMINT-3318113.
STRING9606.ENSP00000253719.

Protein family/group databases

MEROPSA01.046.

PTM databases

PhosphoSiteO96009.

Proteomic databases

PaxDbO96009.
PRIDEO96009.

Protocols and materials databases

DNASU9476.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000253719; ENSP00000253719; ENSG00000131400.
GeneID9476.
KEGGhsa:9476.
UCSCuc002prx.3. human.

Organism-specific databases

CTD9476.
GeneCardsGC19M050861.
H-InvDBHIX0039966.
HGNCHGNC:13395. NAPSA.
HPACAB009591.
HPA045280.
HPA047236.
HPA047744.
MIM605631. gene.
neXtProtNX_O96009.
PharmGKBPA134891814.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG248684.
HOGENOMHOG000197681.
HOVERGENHBG000482.
InParanoidO96009.
KOK08565.
OMAEYLIQCS.
OrthoDBEOG7HQN88.
PhylomeDBO96009.
TreeFamTF314990.

Gene expression databases

ArrayExpressO96009.
BgeeO96009.
CleanExHS_NAPSA.
GenevestigatorO96009.

Family and domain databases

Gene3D2.40.70.10. 2 hits.
InterProIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERPTHR13683. PTHR13683. 1 hit.
PfamPF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
SUPFAMSSF50630. SSF50630. 1 hit.
PROSITEPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNAPSA.
GenomeRNAi9476.
NextBio35512.
PROO96009.
SOURCESearch...

Entry information

Entry nameNAPSA_HUMAN
AccessionPrimary (citable) accession number: O96009
Secondary accession number(s): Q8WWD9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM