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O96009

- NAPSA_HUMAN

UniProt

O96009 - NAPSA_HUMAN

Protein

Napsin-A

Gene

NAPSA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    May be involved in processing of pneumocyte surfactant precursors.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei96 – 961PROSITE-ProRule annotation
    Active sitei283 – 2831PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB
    2. endopeptidase activity Source: UniProtKB
    3. peptidase activity Source: UniProtKB

    GO - Biological processi

    1. membrane protein proteolysis Source: UniProtKB
    2. proteolysis Source: UniProtKB
    3. surfactant homeostasis Source: UniProtKB

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Protein family/group databases

    MEROPSiA01.046.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Napsin-A (EC:3.4.23.-)
    Alternative name(s):
    Aspartyl protease 4
    Short name:
    ASP4
    Short name:
    Asp 4
    Napsin-1
    TA01/TA02
    Gene namesi
    Name:NAPSA
    Synonyms:NAP1, NAPA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:13395. NAPSA.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. alveolar lamellar body Source: UniProtKB
    2. extracellular space Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt
    4. lysosome Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134891814.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Propeptidei26 – 6338Activation peptidePRO_0000025996Add
    BLAST
    Chaini64 – 420357Napsin-APRO_0000025997Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi109 ↔ 116By similarity
    Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi274 ↔ 278By similarity
    Disulfide bondi317 ↔ 354By similarity
    Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiO96009.
    PRIDEiO96009.

    PTM databases

    PhosphoSiteiO96009.

    Expressioni

    Tissue specificityi

    Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.

    Gene expression databases

    ArrayExpressiO96009.
    BgeeiO96009.
    CleanExiHS_NAPSA.
    GenevestigatoriO96009.

    Organism-specific databases

    HPAiCAB009591.
    HPA045280.
    HPA047236.
    HPA047744.

    Interactioni

    Protein-protein interaction databases

    BioGridi114861. 2 interactions.
    IntActiO96009. 1 interaction.
    MINTiMINT-3318113.
    STRINGi9606.ENSP00000253719.

    Structurei

    3D structure databases

    ProteinModelPortaliO96009.
    SMRiO96009. Positions 68-401.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248684.
    HOGENOMiHOG000197681.
    HOVERGENiHBG000482.
    InParanoidiO96009.
    KOiK08565.
    OMAiEYLIQCS.
    OrthoDBiEOG7HQN88.
    PhylomeDBiO96009.
    TreeFamiTF314990.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O96009-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP    50
    AELPKLGAPS PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS 100
    NLWVPSRRCH FFSVPCWLHH RFDPKASSSF QANGTKFAIQ YGTGRVDGIL 150
    SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR 200
    PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP AHYIPPLTFV 250
    PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH 300
    AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG 350
    VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR 400
    ARTRGADLGW GETAQAQFPG 420
    Length:420
    Mass (Da):45,387
    Last modified:May 1, 1999 - v1
    Checksum:i018B86AE5BD0C865
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti40 – 401I → T.1 Publication
    Corresponds to variant rs676314 [ dbSNP | Ensembl ].
    VAR_051510
    Natural varianti310 – 3101A → T.
    Corresponds to variant rs11670727 [ dbSNP | Ensembl ].
    VAR_024586

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090386 mRNA. Translation: AAD04917.1.
    AF200345 mRNA. Translation: AAF17081.1.
    AF098484 mRNA. Translation: AAD13215.1.
    BC017842 mRNA. Translation: AAH17842.1.
    CCDSiCCDS12794.1.
    RefSeqiNP_004842.1. NM_004851.1.
    UniGeneiHs.512843.

    Genome annotation databases

    EnsembliENST00000253719; ENSP00000253719; ENSG00000131400.
    GeneIDi9476.
    KEGGihsa:9476.
    UCSCiuc002prx.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF090386 mRNA. Translation: AAD04917.1 .
    AF200345 mRNA. Translation: AAF17081.1 .
    AF098484 mRNA. Translation: AAD13215.1 .
    BC017842 mRNA. Translation: AAH17842.1 .
    CCDSi CCDS12794.1.
    RefSeqi NP_004842.1. NM_004851.1.
    UniGenei Hs.512843.

    3D structure databases

    ProteinModelPortali O96009.
    SMRi O96009. Positions 68-401.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114861. 2 interactions.
    IntActi O96009. 1 interaction.
    MINTi MINT-3318113.
    STRINGi 9606.ENSP00000253719.

    Protein family/group databases

    MEROPSi A01.046.

    PTM databases

    PhosphoSitei O96009.

    Proteomic databases

    PaxDbi O96009.
    PRIDEi O96009.

    Protocols and materials databases

    DNASUi 9476.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000253719 ; ENSP00000253719 ; ENSG00000131400 .
    GeneIDi 9476.
    KEGGi hsa:9476.
    UCSCi uc002prx.3. human.

    Organism-specific databases

    CTDi 9476.
    GeneCardsi GC19M050861.
    H-InvDB HIX0039966.
    HGNCi HGNC:13395. NAPSA.
    HPAi CAB009591.
    HPA045280.
    HPA047236.
    HPA047744.
    MIMi 605631. gene.
    neXtProti NX_O96009.
    PharmGKBi PA134891814.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG248684.
    HOGENOMi HOG000197681.
    HOVERGENi HBG000482.
    InParanoidi O96009.
    KOi K08565.
    OMAi EYLIQCS.
    OrthoDBi EOG7HQN88.
    PhylomeDBi O96009.
    TreeFami TF314990.

    Miscellaneous databases

    GeneWikii NAPSA.
    GenomeRNAii 9476.
    NextBioi 35512.
    PROi O96009.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O96009.
    Bgeei O96009.
    CleanExi HS_NAPSA.
    Genevestigatori O96009.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Napsins: new human aspartic proteinases. Distinction between two closely related genes."
      Tatnell P.J., Powell D.J., Hill J., Smith T.S., Tew D.G., Kay J.
      FEBS Lett. 441:43-48(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney and Lung.
    2. "Napsin A, a member of the aspartic protease family, is abundantly expressed in normal lung and kidney tissue and is expressed in lung adenocarcinomas."
      Chuman Y., Bergman A.-C., Ueno T., Saito S., Sakaguchi K., Alaiya A.A., Franzen B., Bergman T., Arnott D., Auer G., Appella E., Joernvall H., Linder S.
      FEBS Lett. 462:129-134(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fetal lung.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "New human aspartic proteases napsin 1 and napsin 2: molecular cloning and intracellular localization of napsin 1."
      Koelsch G., Wu S., Henthorn J., Tang J., Lin X.
      Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-40.
      Tissue: Lung.

    Entry informationi

    Entry nameiNAPSA_HUMAN
    AccessioniPrimary (citable) accession number: O96009
    Secondary accession number(s): Q8WWD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3