Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Napsin-A

Gene

NAPSA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in processing of pneumocyte surfactant precursors.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96PROSITE-ProRule annotation1
Active sitei283PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: UniProtKB
  • endopeptidase activity Source: UniProtKB
  • peptidase activity Source: UniProtKB

GO - Biological processi

  • cellular protein metabolic process Source: Reactome
  • membrane protein proteolysis Source: UniProtKB
  • protein catabolic process Source: GO_Central
  • proteolysis Source: UniProtKB
  • surfactant homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.23.B1. 2681.
ReactomeiR-HSA-5683826. Surfactant metabolism.

Protein family/group databases

MEROPSiA01.046.

Names & Taxonomyi

Protein namesi
Recommended name:
Napsin-A (EC:3.4.23.-)
Alternative name(s):
Aspartyl protease 4
Short name:
ASP4
Short name:
Asp 4
Napsin-1
TA01/TA02
Gene namesi
Name:NAPSA
Synonyms:NAP1, NAPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13395. NAPSA.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • lysosome Source: UniProtKB
  • multivesicular body lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi9476.
OpenTargetsiENSG00000131400.
PharmGKBiPA134891814.

Polymorphism and mutation databases

BioMutaiNAPSA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
PropeptideiPRO_000002599626 – 63Activation peptideAdd BLAST38
ChainiPRO_000002599764 – 420Napsin-AAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi90N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi109 ↔ 116By similarity
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi274 ↔ 278By similarity
Disulfide bondi317 ↔ 354By similarity
Glycosylationi336N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO96009.
PaxDbiO96009.
PeptideAtlasiO96009.
PRIDEiO96009.

PTM databases

iPTMnetiO96009.
PhosphoSitePlusiO96009.
SwissPalmiO96009.

Expressioni

Tissue specificityi

Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.

Gene expression databases

BgeeiENSG00000131400.
CleanExiHS_NAPSA.
ExpressionAtlasiO96009. baseline and differential.
GenevisibleiO96009. HS.

Organism-specific databases

HPAiCAB009591.
HPA045280.
HPA047236.
HPA047744.

Interactioni

Protein-protein interaction databases

BioGridi114861. 6 interactors.
IntActiO96009. 1 interactor.
MINTiMINT-3318113.
STRINGi9606.ENSP00000253719.

Structurei

3D structure databases

ProteinModelPortaliO96009.
SMRiO96009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 399Peptidase A1PROSITE-ProRule annotationAdd BLAST322

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO96009.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG091G0JP7.
PhylomeDBiO96009.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O96009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPPLLQPL LLLLPLLNVE PSGATLIRIP LHRVQPGRRI LNLLRGWREP
60 70 80 90 100
AELPKLGAPS PGDKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS
110 120 130 140 150
NLWVPSRRCH FFSVPCWLHH RFDPKASSSF QANGTKFAIQ YGTGRVDGIL
160 170 180 190 200
SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR
210 220 230 240 250
PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP AHYIPPLTFV
260 270 280 290 300
PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH
310 320 330 340 350
AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG
360 370 380 390 400
VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR
410 420
ARTRGADLGW GETAQAQFPG
Length:420
Mass (Da):45,387
Last modified:May 1, 1999 - v1
Checksum:i018B86AE5BD0C865
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05151040I → T.1 PublicationCorresponds to variant rs676314dbSNPEnsembl.1
Natural variantiVAR_024586310A → T.Corresponds to variant rs11670727dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090386 mRNA. Translation: AAD04917.1.
AF200345 mRNA. Translation: AAF17081.1.
AF098484 mRNA. Translation: AAD13215.1.
BC017842 mRNA. Translation: AAH17842.1.
CCDSiCCDS12794.1.
RefSeqiNP_004842.1. NM_004851.2.
XP_011525842.1. XM_011527540.1.
UniGeneiHs.512843.

Genome annotation databases

EnsembliENST00000253719; ENSP00000253719; ENSG00000131400.
GeneIDi9476.
KEGGihsa:9476.
UCSCiuc002prx.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090386 mRNA. Translation: AAD04917.1.
AF200345 mRNA. Translation: AAF17081.1.
AF098484 mRNA. Translation: AAD13215.1.
BC017842 mRNA. Translation: AAH17842.1.
CCDSiCCDS12794.1.
RefSeqiNP_004842.1. NM_004851.2.
XP_011525842.1. XM_011527540.1.
UniGeneiHs.512843.

3D structure databases

ProteinModelPortaliO96009.
SMRiO96009.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114861. 6 interactors.
IntActiO96009. 1 interactor.
MINTiMINT-3318113.
STRINGi9606.ENSP00000253719.

Protein family/group databases

MEROPSiA01.046.

PTM databases

iPTMnetiO96009.
PhosphoSitePlusiO96009.
SwissPalmiO96009.

Polymorphism and mutation databases

BioMutaiNAPSA.

Proteomic databases

EPDiO96009.
PaxDbiO96009.
PeptideAtlasiO96009.
PRIDEiO96009.

Protocols and materials databases

DNASUi9476.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253719; ENSP00000253719; ENSG00000131400.
GeneIDi9476.
KEGGihsa:9476.
UCSCiuc002prx.4. human.

Organism-specific databases

CTDi9476.
DisGeNETi9476.
GeneCardsiNAPSA.
H-InvDBHIX0039966.
HGNCiHGNC:13395. NAPSA.
HPAiCAB009591.
HPA045280.
HPA047236.
HPA047744.
MIMi605631. gene.
neXtProtiNX_O96009.
OpenTargetsiENSG00000131400.
PharmGKBiPA134891814.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiO96009.
KOiK08565.
OMAiIILCSEI.
OrthoDBiEOG091G0JP7.
PhylomeDBiO96009.
TreeFamiTF314990.

Enzyme and pathway databases

BRENDAi3.4.23.B1. 2681.
ReactomeiR-HSA-5683826. Surfactant metabolism.

Miscellaneous databases

GeneWikiiNAPSA.
GenomeRNAii9476.
PROiO96009.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131400.
CleanExiHS_NAPSA.
ExpressionAtlasiO96009. baseline and differential.
GenevisibleiO96009. HS.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAPSA_HUMAN
AccessioniPrimary (citable) accession number: O96009
Secondary accession number(s): Q8WWD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.