##gff-version 3
O96006	UniProtKB	Chain	1	694	.	.	.	ID=PRO_0000066560;Note=E3 SUMO-protein ligase ZBED1	
O96006	UniProtKB	Zinc finger	20	78	.	.	.	Note=BED-type;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00027	
O96006	UniProtKB	Region	315	694	.	.	.	Note=Required for interaction with human adenovirus early E1A protein;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:25210186;Dbxref=PMID:25210186	
O96006	UniProtKB	Region	552	624	.	.	.	Note=Required for DNA binding;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Region	571	651	.	.	.	Note=Required for nuclear localization and homodimerization;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Region	652	694	.	.	.	Note=Required for the formation of higher order complexes of ZBED1 with consensus DNA binding sequences;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Motif	530	534	.	.	.	Note=Nuclear localization signal;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Binding site	47	47	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00027	
O96006	UniProtKB	Binding site	50	50	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00027	
O96006	UniProtKB	Binding site	66	66	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00027	
O96006	UniProtKB	Binding site	71	71	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00027	
O96006	UniProtKB	Mutagenesis	47	47	.	.	.	Note=Abolishes autosumoylation. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27068747;Dbxref=PMID:27068747	
O96006	UniProtKB	Mutagenesis	50	50	.	.	.	Note=Abolishes autosumoylation. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27068747;Dbxref=PMID:27068747	
O96006	UniProtKB	Mutagenesis	71	71	.	.	.	Note=Abolishes autosumoylation. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27068747;Dbxref=PMID:27068747	
O96006	UniProtKB	Mutagenesis	360	360	.	.	.	Note=Abolishes interaction with SUMO1. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27068747;Dbxref=PMID:27068747	
O96006	UniProtKB	Mutagenesis	401	401	.	.	.	Note=Abolishes interaction with SUMO1. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:27068747;Dbxref=PMID:27068747	
O96006	UniProtKB	Mutagenesis	530	531	.	.	.	Note=Abolishes nuclear localization%2C however has no effect on homodimerization%3B when associated with A-534. KK->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Mutagenesis	534	534	.	.	.	Note=Abolishes nuclear localization%2C however has no effect on homodimerization%3B when associated with A-530-A-A-531. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Mutagenesis	590	591	.	.	.	Note=Abolishes homodimerization%2C multimerization%2C interaction with KPNB1%2C nuclear localization and DNA binding activity. Abolishes interaction with SUMO1 and reduces SUMOylation of CHD3/Mi2-alpha. WW->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17209048,ECO:0000269|PubMed:27068747;Dbxref=PMID:17209048,PMID:27068747	
O96006	UniProtKB	Mutagenesis	599	599	.	.	.	Note=No effect on homodimerization or nuclear localization. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Mutagenesis	600	601	.	.	.	Note=Abolishes homodimerization and nuclear localization. Abolishes homodimerization%2C multimerization%2C nuclear localization%2C and DNA binding activity%3B when associated with A-604-A-A-605. Abolishes interaction with SUMO1 and reduces sumoylation of CHD3/Mi2-alpha%3B when associated with 604-A-A-605. LL->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17209048,ECO:0000269|PubMed:27068747;Dbxref=PMID:17209048,PMID:27068747	
O96006	UniProtKB	Mutagenesis	604	605	.	.	.	Note=Reduces homodimerization and nuclear localization. Abolishes homodimerization%2C multimerization%2C nuclear localization%2C and DNA binding activity%3B when associated with A-600-A-A-601. Abolishes interaction with SUMO1 and reduces sumoylation of CHD3/Mi2-alpha%3B when associated with A-600-A-A-601. VL->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17209048,ECO:0000269|PubMed:27068747;Dbxref=PMID:17209048,PMID:27068747	
O96006	UniProtKB	Mutagenesis	619	620	.	.	.	Note=No effect on homodimerization or nuclear localization. ER->AA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Mutagenesis	622	622	.	.	.	Note=No effect on homodimerization or nuclear localization. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Mutagenesis	633	633	.	.	.	Note=No effect on homodimerization or nuclear localization. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17209048;Dbxref=PMID:17209048	
O96006	UniProtKB	Sequence conflict	295	295	.	.	.	Note=F->L;Ontology_term=ECO:0000305;evidence=ECO:0000305	
O96006	UniProtKB	Beta strand	27	29	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5	
O96006	UniProtKB	Beta strand	35	37	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5	
O96006	UniProtKB	Helix	39	41	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5	
O96006	UniProtKB	Turn	48	51	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5	
O96006	UniProtKB	Helix	61	69	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5	
O96006	UniProtKB	Helix	72	78	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:2CT5