O95998 (I18BP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-18-binding protein
Short name=IL-18BP

Alternative name(s):
Tadekinig-alfa

Gene names

Name:

IL18BP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. Ref.1 Ref.4
Subcellular location	Secreted Potential.
Tissue specificity	Strongly expressed in heart, lung, placenta and spleen. Ref.1 Ref.2
Post-translational modification	N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4. Ref.10
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Sequence caution	The sequence AAD17187.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD17188.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD17189.1 differs from that shown. Reason: Intron retention. The sequence AAD17190.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD17191.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD17192.1 differs from that shown. Reason: Intron retention. The sequence AAF31697.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	T-helper 1 type immune response Inferred from direct assay Ref.1. Source: UniProtKB cellular response to hydrogen peroxide Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Traceable author statement Ref.1. Source: UniProtKB extracellular space Inferred from electronic annotation. Source: Compara
Molecular_function	interleukin-18 binding Inferred from direct assay Ref.1. Source: UniProtKB receptor antagonist activity Traceable author statement PubMed 11890646. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: O95998-2)

Also known as: IL-18BPA;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: O95998-3)

Also known as: IL-18BPB;

The sequence of this isoform differs from the canonical sequence as follows:
     79-115: NGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLW → SWAEGNLAPHPRSPALQPQQSTAAGLRLSTGPAAAQP
     116-194: Missing.

Isoform D (identifier: O95998-4)

Also known as: IL-18BPD;

The sequence of this isoform differs from the canonical sequence as follows:
     128-163: TQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHV → WAEGNLAPHPRSPALQPQQSTAAGLRLSTGPAAAQP
     164-194: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.1 Ref.2 Ref.9
	Chain	31 – 194	164	Interleukin-18-binding protein		PRO_0000014778
Regions
	Domain	65 – 166	102	Ig-like C2-type
Amino acid modifications
	Glycosylation	53	1	O-linked (GalNAc...) Ref.10
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) (complex) Ref.10
	Glycosylation	147	1	N-linked (GlcNAc...) (complex) Ref.10
	Disulfide bond	86 ↔ 150		By similarity
Natural variations
	Alternative sequence	79 – 115	37	NGTLS…PGRLW → SWAEGNLAPHPRSPALQPQQ STAAGLRLSTGPAAAQP in isoform B.		VSP_002515
	Alternative sequence	116 – 194	79	Missing in isoform B.		VSP_002516
	Alternative sequence	128 – 163	36	TQLCK…VQRHV → WAEGNLAPHPRSPALQPQQS TAAGLRLSTGPAAAQP in isoform D.		VSP_037605
	Alternative sequence	164 – 194	31	Missing in isoform D.		VSP_037606
	Natural variant	91	1	R → H. Corresponds to variant rs5743672 [ dbSNP | Ensembl ].		VAR_059393
	Natural variant	121	1	R → Q. Corresponds to variant rs5743673 [ dbSNP | Ensembl ].		VAR_024497

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (IL-18BPA) [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 5E520D6E46AFA843	FASTA	194	21,099
	10 20 30 40 50 60 MTMRHNWTPD LSPLWVLLLC AHVVTLLVRA TPVSQTTTAA TASVRSTKDP CPSQPPVFPA 70 80 90 100 110 120 AKQCPALEVT WPEVEVPLNG TLSLSCVACS RFPNFSILYW LGNGSFIEHL PGRLWEGSTS 130 140 150 160 170 180 RERGSTGTQL CKALVLEQLT PALHSTNFSC VLVDPEQVVQ RHVVLAQLWA GLRATLPPTQ 190 EALPSSHSSP QQQG
	Isoform B (IL-18BPB) [UniParc]. Checksum: 2D6C79F64E1937BF	FASTA	115	12,221
	10 20 30 40 50 60 MTMRHNWTPD LSPLWVLLLC AHVVTLLVRA TPVSQTTTAA TASVRSTKDP CPSQPPVFPA 70 80 90 100 110 AKQCPALEVT WPEVEVPLSW AEGNLAPHPR SPALQPQQST AAGLRLSTGP AAAQP
	Isoform D (IL-18BPD) [UniParc]. Checksum: D25DD52998594ABB	FASTA	163	17,478
	10 20 30 40 50 60 MTMRHNWTPD LSPLWVLLLC AHVVTLLVRA TPVSQTTTAA TASVRSTKDP CPSQPPVFPA 70 80 90 100 110 120 AKQCPALEVT WPEVEVPLNG TLSLSCVACS RFPNFSILYW LGNGSFIEHL PGRLWEGSTS 130 140 150 160 RERGSTGWAE GNLAPHPRSP ALQPQQSTAA GLRLSTGPAA AQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response." Novick D., Kim S.-H., Fantuzzi G., Reznikov L.L., Dinarello C.A., Rubinstein M. Immunity 10:127-136(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS A AND B), PROTEIN SEQUENCE OF 31-70, FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"Cloning and expression of interleukin-18 binding protein." Aizawa Y., Akita K., Taniai M., Torigoe K., Mori T., Nishida Y., Ushio S., Nukada Y., Tanimoto T., Ikegami H., Ikeda M., Kurimoto M. FEBS Lett. 445:338-342(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), PROTEIN SEQUENCE OF 31-35; 39-45; 48-54; 57-60; 63-74; 91-95; 107-144 AND 151-169, TISSUE SPECIFICITY.
[3]	"Identification of human and mouse homologs of the MC51L-53L-54L family of secreted glycoproteins encoded by the Molluscum contagiosum poxvirus." Xiang Y., Moss B. Virology 257:297-302(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[4]	"Structural requirements of six naturally occurring isoforms of the IL-18 binding protein to inhibit IL-18." Kim S.-H., Eisenstein M., Reznikov L., Fantuzzi G., Novick D., Rubinstein M., Dinarello C.A. Proc. Natl. Acad. Sci. U.S.A. 97:1190-1195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D), FUNCTION, ALTERNATIVE SPLICING (ISOFORMS A AND B). Tissue: T-cell.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Uterus.
[6]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Tissue: Leukocyte.
[9]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-45.
[10]	"Human urinary glycoproteomics; attachment site specific analysis of N-and O-linked glycosylations by CID and ECD." Halim A., Nilsson J., Ruetschi U., Hesse C., Larson G. Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT SER-53; ASN-103 AND ASN-147, STRUCTURE OF CARBOHYDRATES, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110798 Genomic DNA. Translation: AAD17187.1. Different initiation. AF110798 Genomic DNA. Translation: AAD17188.1. Different initiation. AF110798 Genomic DNA. Translation: AAD17189.1. Sequence problems. AF110799 mRNA. Translation: AAD17190.1. Different initiation. AF110800 mRNA. Translation: AAD17191.1. Different initiation. AF110801 mRNA. Translation: AAD17192.1. Sequence problems. AB019504 mRNA. Translation: BAA76374.1. AF122906 mRNA. Translation: AAD41051.1. AF215907 mRNA. Translation: AAF31697.1. Different initiation. AK098255 mRNA. Translation: BAG53602.1. AP002490 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW74813.1. CH471076 Genomic DNA. Translation: EAW74816.1. BC044215 mRNA. Translation: AAH44215.1.
IPI	IPI00304305. IPI00848010. IPI00873924.
RefSeq	NP_001034748.1. NM_001039659.1. NP_001034749.1. NM_001039660.1. NP_001138527.1. NM_001145055.1. NP_001138529.1. NM_001145057.1. NP_005690.2. NM_005699.3. NP_766630.2. NM_173042.2. NP_766632.2. NM_173044.2.
UniGene	Hs.591967.
3D structure databases
ProteinModelPortal	O95998.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95998. 3 interactions.
STRING	9606.ENSP00000260049.
Proteomic databases
PaxDb	O95998.
PRIDE	O95998.
Protocols and materials databases
DNASU	10068.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260049; ENSP00000260049; ENSG00000137496. ENST00000337131; ENSP00000338723; ENSG00000137496. ENST00000343898; ENSP00000343309; ENSG00000137496. ENST00000393703; ENSP00000377306; ENSG00000137496. ENST00000393705; ENSP00000377308; ENSG00000137496. ENST00000393707; ENSP00000377310; ENSG00000137496. ENST00000404792; ENSP00000384212; ENSG00000137496. ENST00000534583; ENSP00000434376; ENSG00000137496.
GeneID	10068.
KEGG	hsa:10068.
UCSC	uc001ore.1. human. uc009ysv.1. human. uc021qmv.1. human.
Organism-specific databases
CTD	10068.
GeneCards	GC11P071709.
H-InvDB	HIX0009903.
HGNC	HGNC:5987. IL18BP.
HPA	CAB025980.
MIM	604113. gene.
neXtProt	NX_O95998.
PharmGKB	PA29803.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45302.
HOVERGEN	HBG052064.
OMA	CTACSRF.
OrthoDB	EOG4DFPPS.
Gene expression databases
ArrayExpress	O95998.
Bgee	O95998.
CleanEx	HS_IL18BP.
Genevestigator	O95998.
GermOnline	ENSG00000137496. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like_dom. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10068.
NextBio	38051.
SOURCE	Search...

Entry information

Entry name

I18BP_HUMAN

Accession

Primary (citable) accession number: O95998
Secondary accession number(s): B3KUZ0 , B7WPK4, O95993, O96027, Q9NZA9, Q9UBR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.