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O95996 (APC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenomatous polyposis coli protein 2
Alternative name(s):
Adenomatous polyposis coli protein-like
Short name=APC-like
Gene names
Name:APC2
Synonyms:APCL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2303 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. Ref.1 Ref.5 Ref.9

Subunit structure

Interacts with PSRC1 By similarity. Interacts with APC, CTNNB1, MAPRE1, MAPRE3, TP53BP2 and possibly with AXIN2. Ref.1 Ref.5 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasmcytoskeleton. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with actin filaments and the microtubule network. Ref.7 Ref.8 Ref.9

Tissue specificity

Widely expressed (at protein level). Specifically expressed in the CNS. Ref.1 Ref.5 Ref.9

Developmental stage

Expressed in fetal brain. Ref.5

Sequence similarities

Belongs to the adenomatous polyposis coli (APC) family.

Contains 6 ARM repeats.

Sequence caution

The sequence AAD28183.1 differs from that shown. Reason: Frameshift at positions 1322 and 1338.

The sequence AAF01784.1 differs from that shown. Reason: Contaminating sequence.

The sequence CAA10317.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB61207.1 differs from that shown. Reason: Contaminating sequence.

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95996-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95996-2)

The sequence of this isoform differs from the canonical sequence as follows:
     168-441: Missing.
Isoform 3 (identifier: O95996-3)

The sequence of this isoform differs from the canonical sequence as follows:
     175-175: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 23032303Adenomatous polyposis coli protein 2
PRO_0000313686

Regions

Repeat302 – 34140ARM 1
Repeat479 – 51840ARM 2
Repeat522 – 56241ARM 3
Repeat566 – 60944ARM 4
Repeat615 – 65440ARM 5
Repeat657 – 69640ARM 6
Repeat1058 – 1077201
Repeat1150 – 1169202
Repeat1263 – 1282203
Repeat1391 – 1410204
Repeat1568 – 1587205
Region1058 – 15875305 X 20 AA approximate repeat of F-X-V-E-X-T-P-X-C-F-S-R-X-S-S-L-S-S-L-S
Region1058 – 1587530Interaction with CTNNB1
Region2067 – 214478Interaction with MAPRE1 and MAPRE3
Coiled coil8 – 5952 Potential
Coiled coil840 – 86425 Potential
Compositional bias1145 – 119652Ser-rich
Compositional bias1518 – 1622105Pro-rich
Compositional bias1765 – 1953189Pro-rich

Natural variations

Alternative sequence168 – 441274Missing in isoform 2.
VSP_030106
Alternative sequence1751Missing in isoform 3.
VSP_030107
Natural variant5621A → S in a breast cancer sample; somatic mutation. Ref.10
VAR_037703
Natural variant20031G → S in a breast cancer sample; somatic mutation. Ref.10
VAR_037704
Natural variant22411S → A.
Corresponds to variant rs265277 [ dbSNP | Ensembl ].
VAR_037705

Experimental info

Sequence conflict4631Missing in AAD28183. Ref.4
Sequence conflict526 – 5272KV → NL in AAD28183. Ref.4
Sequence conflict526 – 5272KV → NL in AAD29274. Ref.4
Sequence conflict5661E → A in AAF01784. Ref.5
Sequence conflict5661E → A in CAB61207. Ref.5
Sequence conflict8161L → Q in AAD28183. Ref.4
Sequence conflict9711L → P in AAD28183. Ref.4
Sequence conflict11061S → I in AAD28183. Ref.4
Sequence conflict11401E → G in CAA10317. Ref.5
Sequence conflict11881Q → H in AAD28183. Ref.4
Sequence conflict13611A → G in AAD28183. Ref.4
Sequence conflict14561R → P in AAD28183. Ref.4
Sequence conflict15151D → V in AAD28183. Ref.4
Sequence conflict22191A → V in CAA10317. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 7BF940183ACD643D

FASTA2,303243,949
        10         20         30         40         50         60 
MASSVAPYEQ LVRQVEALKA ENSHLRQELR DNSSHLSKLE TETSGMKEVL KHLQGKLEQE 

        70         80         90        100        110        120 
ARVLVSSGQT EVLEQLKALQ MDITSLYNLK FQPPTLGPEP AARTPEGSPV HGSGPSKDSF 

       130        140        150        160        170        180 
GELSRATIRL LEELDRERCF LLNEIEKEEK EKLWYYSQLQ GLSKRLDELP HVETQFSMQM 

       190        200        210        220        230        240 
DLIRQQLEFE AQHIRSLMEE RFGTSDEMVQ RAQIRASRLE QIDKELLEAQ DRVQQTEPQA 

       250        260        270        280        290        300 
LLAVKSVPVD EDPETEVPTH PEDGTPQPGN SKVEVVFWLL SMLATRDQED TARTLLAMSS 

       310        320        330        340        350        360 
SPESCVAMRR SGCLPLLLQI LHGTEAAAGG RAGAPGAPGA KDARMRANAA LHNIVFSQPD 

       370        380        390        400        410        420 
QGLARKEMRV LHVLEQIRAY CETCWDWLQA RDGGPEGGGA GSAPIPIEPQ ICQATCAVMK 

       430        440        450        460        470        480 
LSFDEEYRRA MNELGGLQAV AELLQVDYEM HKMTRDPLNL ALRRYAGMTL TNLTFGDVAN 

       490        500        510        520        530        540 
KATLCARRGC MEAIVAQLAS DSEELHQVVS SILRNLSWRA DINSKKVLRE AGSVTALVQC 

       550        560        570        580        590        600 
VLRATKESTL KSVLSALWNL SAHSTENKAA ICQVDGALGF LVSTLTYKCQ SNSLAIIESG 

       610        620        630        640        650        660 
GGILRNVSSL VATREDYRQV LRDHNCLQTL LQHLTSHSLT IVSNACGTLW NLSARSARDQ 

       670        680        690        700        710        720 
ELLWDLGAVG MLRNLVHSKH KMIAMGSAAA LRNLLAHRPA KHQAAATAVS PGSCVPSLYV 

       730        740        750        760        770        780 
RKQRALEAEL DARHLAQALE HLEKQGPPAA EAATKKPLPP LRHLDGLAQD YASDSGCFDD 

       790        800        810        820        830        840 
DDAPSSLAAA AATGEPASPA ALSLFLGSPF LQGQALARTP PTRRGGKEAE KDTSGEAAVA 

       850        860        870        880        890        900 
AKAKAKLALA VARIDQLVED ISALHTSSDD SFSLSSGDPG QEAPREGRAQ SCSPCRGPEG 

       910        920        930        940        950        960 
GRREAGSRAH PLLRLKAAHA SLSNDSLNSG SASDGYCPRE HMLPCPLAAL ASRREDPRCG 

       970        980        990       1000       1010       1020 
QPRPSRLDLD LPGCQAEPPA REATSADARV RTIKLSPTYQ HVPLLEGASR AGAEPLAGPG 

      1030       1040       1050       1060       1070       1080 
ISPGARKQAW LPADHLSKVP EKLAAAPLSV ASKALQKLAA QEGPLSLSRC SSLSSLSSAG 

      1090       1100       1110       1120       1130       1140 
RPGPSEGGDL DDSDSSLEGL EEAGPSEAEL DSTWRAPGAT SLPVAIPAPR RNRGRGLGVE 

      1150       1160       1170       1180       1190       1200 
DATPSSSSEN YVQETPLVLS RCSSVSSLGS FESPSIASSI PSEPCSGQGS GTISPSELPD 

      1210       1220       1230       1240       1250       1260 
SPGQTMPPSR SKTPPLAPAP QGPPEATQFS LQWESYVKRF LDIADCRERC RLPSELDAGS 

      1270       1280       1290       1300       1310       1320 
VRFTVEKPDE NFSCASSLSA LALHEHYVQQ DVELRLLPSA CPERGGGAGG AGLHFAGHRR 

      1330       1340       1350       1360       1370       1380 
REEGPAPTGS RPRGAADQEL ELLRECLGAA VPARLRKVAS ALVPGRRALP VPVYMLVPAP 

      1390       1400       1410       1420       1430       1440 
APAQEDDSCT DSAEGTPVNF SSAASLSDET LQGPPRDQPG GPAGRQRPTG RPTSARQAMG 

      1450       1460       1470       1480       1490       1500 
HRHKAGGAGR SAEQSRGAGK NRAGLELPLG RPPSAPADKD GSKPGRTRGD GALQSLCLTT 

      1510       1520       1530       1540       1550       1560 
PTEEAVYCFY GNDSDEEPPA AAPTPTHRRT SAIPRAFTRE RPQGRKEAPA PSKAAPAAPP 

      1570       1580       1590       1600       1610       1620 
PARTQPSLIA DETPPCYSLS SSASSLSEPE PSEPPAVHPR GREPAVTKDP GPGGGRDSSP 

      1630       1640       1650       1660       1670       1680 
SPRAAEELLQ RCISSALPRR RPPVSGLRRR KPRATRLDER PAEGSRERGE EAAGSDRASD 

      1690       1700       1710       1720       1730       1740 
LDSVEWRAIQ EGANSIVTWL HQAAAATREA SSESDSILSF VSGLSVGSTL QPPKHRKGRQ 

      1750       1760       1770       1780       1790       1800 
AEGEMGSARR PEKRGAASVK TSGSPRSPAG PEKPRGTQKT TPGVPAVLRG RTVIYVPSPA 

      1810       1820       1830       1840       1850       1860 
PRAQPKGTPG PRATPRKVAP PCLAQPAAPA KVPSPGQQRS RSLHRPAKTS ELATLSQPPR 

      1870       1880       1890       1900       1910       1920 
SATPPARLAK TPSSSSSQTS PASQPLPRKR PPVTQAAGAL PGPGASPVPK TPARTLLAKQ 

      1930       1940       1950       1960       1970       1980 
HKTQRSPVRI PFMQRPARRG PPPLARAVPE PGPRGRAGTE AGPGARGGRL GLVRVASALS 

      1990       2000       2010       2020       2030       2040 
SGSESSDRSG FRRQLTFIKE SPGLRRRRSE LSSAESAASA PQGASPRRGR PALPAVFLCS 

      2050       2060       2070       2080       2090       2100 
SRCEELRAAP RQGPAPARQR PPAARPSPGE RPARRTTSES PSRLPVRAPA ARPETVKRYA 

      2110       2120       2130       2140       2150       2160 
SLPHISVARR PDGAVPAAPA SADAARRSSD GEPRPLPRVA APGTTWRRIR DEDVPHILRS 

      2170       2180       2190       2200       2210       2220 
TLPATALPLR GSTPEDAPAG PPPRKTSDAV VQTEEVAAPK TNSSTSPSLE TREPPGAPAG 

      2230       2240       2250       2260       2270       2280 
GQLSLLGSDV DGPSLAKAPI SAPFVHEGLG VAVGGFPASR HGSPSRSARV PPFNYVPSPM 

      2290       2300 
VVAATTDSAA EKAPATASAT LLE 

« Hide

Isoform 2 [UniParc].

Checksum: F509BD1F39C43FFF
Show »

FASTA2,029213,799
Isoform 3 [UniParc].

Checksum: BD396DB4248091F4
Show »

FASTA2,302243,821

References

« Hide 'large scale' references
[1]"Identification of a brain-specific APC homologue, APCL, and its interaction with beta-catenin."
Nakagawa H., Murata Y., Koyama K., Fujiyama A., Miyoshi Y., Monden M., Akiyama T., Nakamura Y.
Cancer Res. 58:5176-5181(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH CTNNB1, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"Analysis of APCL, a brain-specific adenomatous polyposis coli homologue, for mutations and expression in brain tumors."
Nakagawa H., Koyama K., Monden M., Nakamura Y.
Jpn. J. Cancer Res. 90:982-986(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"APC2 alternatively spliced cDNA sequence."
Carr I.M., Markham A.F., Colleta P.L., Wai L., Askham J., Morrison E., Meredith D.M.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-1521 (ISOFORM 2), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-86 AND 476-555 (ISOFORM 2).
[5]"Identification of APC2, a homologue of the adenomatous polyposis coli tumour suppressor."
van Es J.H., Kirkpatrick C., van de Wetering M., Molenaar M., Miles A., Kuipers J., Destree O., Peifer M., Clevers H.
Curr. Biol. 9:105-108(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-731 (ISOFORM 3), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 618-2303 (ISOFORM 3), FUNCTION, INTERACTION WITH AXIN2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Kidney.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-702 (ISOFORM 1).
Tissue: Brain.
[7]"APCL, a central nervous system-specific homologue of adenomatous polyposis coli tumor suppressor, binds to p53-binding protein 2 and translocates it to the perinucleus."
Nakagawa H., Koyama K., Murata Y., Morito M., Akiyama T., Nakamura Y.
Cancer Res. 60:101-105(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TP53BP2, SUBCELLULAR LOCATION.
[8]"EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue."
Nakagawa H., Koyama K., Murata Y., Morito M., Akiyama T., Nakamura Y.
Oncogene 19:210-216(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MAPRE1 AND MAPRE3, SUBCELLULAR LOCATION.
Tissue: Fetal brain.
[9]"Human APC2 localization and allelic imbalance."
Jarrett C.R., Blancato J., Cao T., Bressette D.S., Cepeda M., Young P.E., King C.R., Byers S.W.
Cancer Res. 61:7978-7984(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH APC, TISSUE SPECIFICITY.
[10]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] SER-562 AND SER-2003.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB012162 mRNA. Translation: BAA34611.1.
AB022529 Genomic DNA. Translation: BAA75469.1.
CH471139 Genomic DNA. Translation: EAW69498.1.
AF110334 mRNA. Translation: AAD28183.1. Frameshift.
AF110335 Genomic DNA. Translation: AAD29273.1.
AF110337, AF110336 Genomic DNA. Translation: AAD29274.1.
AF128222 mRNA. Translation: AAF01784.1. Sequence problems.
AJ012652 mRNA. Translation: CAB61207.1. Sequence problems.
AJ131187 Genomic DNA. Translation: CAA10317.1. Different initiation.
BC032573 mRNA. Translation: AAH32573.1.
RefSeqNP_005874.1. NM_005883.2.
UniGeneHs.446376.

3D structure databases

ProteinModelPortalO95996.
SMRO95996. Positions 6-57, 131-234, 287-703.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115585. 20 interactions.
IntActO95996. 4 interactions.
STRING9606.ENSP00000233607.

PTM databases

PhosphoSiteO95996.

Proteomic databases

PaxDbO95996.
PRIDEO95996.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000233607; ENSP00000233607; ENSG00000115266. [O95996-1]
ENST00000535453; ENSP00000442954; ENSG00000115266. [O95996-1]
ENST00000590469; ENSP00000467073; ENSG00000115266.
GeneID10297.
KEGGhsa:10297.
UCSCuc002lsr.1. human. [O95996-1]
uc002lsu.1. human. [O95996-3]

Organism-specific databases

CTD10297.
GeneCardsGC19P001450.
H-InvDBHIX0039920.
HGNCHGNC:24036. APC2.
HPAHPA050820.
MIM612034. gene.
neXtProtNX_O95996.
PharmGKBPA134906843.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG259696.
HOVERGENHBG104821.
InParanoidO95996.
KOK02085.
OMASIPSDPC.
OrthoDBEOG7BCN9S.
PhylomeDBO95996.
TreeFamTF106496.

Enzyme and pathway databases

SignaLinkO95996.

Gene expression databases

ArrayExpressO95996.
BgeeO95996.
CleanExHS_APC2.
GenevestigatorO95996.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR026837. APC2.
IPR009234. APC_basic_dom.
IPR009223. APC_Cys-rich_rpt.
IPR026831. APC_dom.
IPR026818. Apc_fam.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR009224. SAMP.
[Graphical view]
PANTHERPTHR12607. PTHR12607. 1 hit.
PTHR12607:SF3. PTHR12607:SF3. 1 hit.
PfamPF05956. APC_basic. 1 hit.
PF05923. APC_crr. 4 hits.
PF00514. Arm. 1 hit.
PF05924. SAMP. 2 hits.
PF11414. Suppressor_APC. 1 hit.
[Graphical view]
SMARTSM00185. ARM. 6 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSAPC2. human.
GenomeRNAi10297.
NextBio39024.
PROO95996.
SOURCESearch...

Entry information

Entry nameAPC2_HUMAN
AccessionPrimary (citable) accession number: O95996
Secondary accession number(s): Q05BW4 expand/collapse secondary AC list , Q9UBZ1, Q9UEM8, Q9UQJ8, Q9UQJ9, Q9Y632
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM