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O95994

- AGR2_HUMAN

UniProt

O95994 - AGR2_HUMAN

Protein

Anterior gradient protein 2 homolog

Gene

AGR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells By similarity. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.By similarity1 Publication

    GO - Molecular functioni

    1. dystroglycan binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. lung goblet cell differentiation Source: Ensembl
    2. mucus secretion Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anterior gradient protein 2 homolog
    Short name:
    AG-2
    Short name:
    hAG-2
    Alternative name(s):
    HPC8
    Secreted cement gland protein XAG-2 homolog
    Gene namesi
    Name:AGR2
    Synonyms:AG2
    ORF Names:UNQ515/PRO1030
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:328. AGR2.

    Subcellular locationi

    Secreted 1 Publication. Endoplasmic reticulum By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. extracellular region Source: UniProtKB-SubCell
    3. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811C → S: Loss of interaction with MUC2. 1 Publication

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    PharmGKBiPA24625.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 175155Anterior gradient protein 2 homologPRO_0000001037Add
    BLAST

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiO95994.
    PaxDbiO95994.
    PRIDEiO95994.

    PTM databases

    PhosphoSiteiO95994.

    Expressioni

    Tissue specificityi

    Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.1 Publication

    Gene expression databases

    ArrayExpressiO95994.
    BgeeiO95994.
    CleanExiHS_AGR2.
    GenevestigatoriO95994.

    Organism-specific databases

    HPAiHPA007912.

    Interactioni

    Subunit structurei

    Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked.2 Publications

    Protein-protein interaction databases

    BioGridi115802. 14 interactions.
    DIPiDIP-48825N.
    IntActiO95994. 7 interactions.
    MINTiMINT-2868509.
    STRINGi9606.ENSP00000391490.

    Structurei

    Secondary structure

    1
    175
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi48 – 514
    Helixi58 – 669
    Turni67 – 693
    Beta strandi72 – 776
    Beta strandi79 – 813
    Helixi82 – 9110
    Helixi95 – 1028
    Beta strandi103 – 1053
    Beta strandi109 – 1113
    Turni116 – 1183
    Beta strandi127 – 1326
    Turni133 – 1353
    Turni146 – 1505
    Turni154 – 1563
    Helixi157 – 16711
    Beta strandi171 – 1733

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LNSNMR-A/B41-175[»]
    2LNTNMR-A41-175[»]
    ProteinModelPortaliO95994.
    SMRiO95994. Positions 38-175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AGR family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG78144.
    HOGENOMiHOG000231100.
    HOVERGENiHBG006516.
    OrthoDBiEOG7DNNX2.
    PhylomeDBiO95994.
    TreeFamiTF321449.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR012336. Thioredoxin-like_fold.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O95994-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKIPVSAFL LLVALSYTLA RDTTVKPGAK KDTKDSRPKL PQTLSRGWGD    50
    QLIWTQTYEE ALYKSKTSNK PLMIIHHLDE CPHSQALKKV FAENKEIQKL 100
    AEQFVLLNLV YETTDKHLSP DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY 150
    AYEPADTALL LDNMKKALKL LKTEL 175
    Length:175
    Mass (Da):19,979
    Last modified:May 1, 1999 - v1
    Checksum:iF271B1BD377BEE11
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007791 mRNA. Translation: AAC77358.1.
    AF038451 mRNA. Translation: AAC82614.1.
    AF088867 mRNA. Translation: AAF22484.1.
    AF115926 mRNA. Translation: AAL54870.1.
    AF087879 mRNA. Translation: AAP97179.1.
    AY359009 mRNA. Translation: AAQ89368.1.
    BT007048 mRNA. Translation: AAP35697.1.
    AC073333 Genomic DNA. Translation: AAP22354.1.
    BC015503 mRNA. Translation: AAH15503.1.
    CCDSiCCDS5364.1.
    PIRiJE0350.
    RefSeqiNP_006399.1. NM_006408.3.
    XP_005249638.1. XM_005249581.2.
    UniGeneiHs.530009.

    Genome annotation databases

    EnsembliENST00000419304; ENSP00000391490; ENSG00000106541.
    GeneIDi10551.
    KEGGihsa:10551.
    UCSCiuc003str.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007791 mRNA. Translation: AAC77358.1 .
    AF038451 mRNA. Translation: AAC82614.1 .
    AF088867 mRNA. Translation: AAF22484.1 .
    AF115926 mRNA. Translation: AAL54870.1 .
    AF087879 mRNA. Translation: AAP97179.1 .
    AY359009 mRNA. Translation: AAQ89368.1 .
    BT007048 mRNA. Translation: AAP35697.1 .
    AC073333 Genomic DNA. Translation: AAP22354.1 .
    BC015503 mRNA. Translation: AAH15503.1 .
    CCDSi CCDS5364.1.
    PIRi JE0350.
    RefSeqi NP_006399.1. NM_006408.3.
    XP_005249638.1. XM_005249581.2.
    UniGenei Hs.530009.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LNS NMR - A/B 41-175 [» ]
    2LNT NMR - A 41-175 [» ]
    ProteinModelPortali O95994.
    SMRi O95994. Positions 38-175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115802. 14 interactions.
    DIPi DIP-48825N.
    IntActi O95994. 7 interactions.
    MINTi MINT-2868509.
    STRINGi 9606.ENSP00000391490.

    PTM databases

    PhosphoSitei O95994.

    Proteomic databases

    MaxQBi O95994.
    PaxDbi O95994.
    PRIDEi O95994.

    Protocols and materials databases

    DNASUi 10551.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000419304 ; ENSP00000391490 ; ENSG00000106541 .
    GeneIDi 10551.
    KEGGi hsa:10551.
    UCSCi uc003str.3. human.

    Organism-specific databases

    CTDi 10551.
    GeneCardsi GC07M016797.
    HGNCi HGNC:328. AGR2.
    HPAi HPA007912.
    MIMi 606358. gene.
    neXtProti NX_O95994.
    PharmGKBi PA24625.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG78144.
    HOGENOMi HOG000231100.
    HOVERGENi HBG006516.
    OrthoDBi EOG7DNNX2.
    PhylomeDBi O95994.
    TreeFami TF321449.

    Miscellaneous databases

    GeneWikii AGR2.
    GenomeRNAii 10551.
    NextBioi 40021.
    PROi O95994.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95994.
    Bgeei O95994.
    CleanExi HS_AGR2.
    Genevestigatori O95994.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "hAG-2, the human homologue of the Xenopus laevis cement gland gene XAG-2, is coexpressed with estrogen receptor in breast cancer cell lines."
      Thompson D.A., Weigel R.J.
      Biochem. Biophys. Res. Commun. 251:111-116(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Mammary gland.
    2. "Identification of human homolog of XAG-2 over-expressed in tumors."
      Zhang J.S., Smith D.I.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Prostatic carcinoma.
    3. "Cloning and expression of a novel human cDNA homology to murine GOB-4 mRNA."
      Fan Y.X., Yu L., Zhang X.N., Wan W.C., Wang X.K., Zhao S.Y.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    8. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-35.
    9. "hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan."
      Fletcher G.C., Patel S., Tyson K., Adam P.J., Schenker M., Loader J.A., Daviet L., Legrain P., Parekh R., Harris A.L., Terrett J.A.
      Br. J. Cancer 88:579-585(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LYPD3 AND DAG1.
    10. "AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer."
      Zhang J.-S., Gong A., Cheville J.C., Smith D.I., Young C.Y.F.
      Genes Chromosomes Cancer 43:249-259(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    11. "The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation."
      Wang Z., Hao Y., Lowe A.W.
      Cancer Res. 68:492-497(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "The protein disulfide isomerase AGR2 is essential for production of intestinal mucus."
      Park S.-W., Zhen G., Verhaeghe C., Nakagami Y., Nguyenvu L.T., Barczak A.J., Killeen N., Erle D.J.
      Proc. Natl. Acad. Sci. U.S.A. 106:6950-6955(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MUC2, MUTAGENESIS OF CYS-81.

    Entry informationi

    Entry nameiAGR2_HUMAN
    AccessioniPrimary (citable) accession number: O95994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3