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O95994 (AGR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anterior gradient protein 2 homolog

Short name=AG-2
Short name=hAG-2
Alternative name(s):
HPC8
Secreted cement gland protein XAG-2 homolog
Gene names
Name:AGR2
Synonyms:AG2
ORF Names:UNQ515/PRO1030
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells By similarity. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Ref.11

Subunit structure

Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Ref.9 Ref.12

Subcellular location

Secreted. Endoplasmic reticulum By similarity Ref.10.

Tissue specificity

Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Ref.1

Sequence similarities

Belongs to the AGR family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.8
Chain21 – 175155Anterior gradient protein 2 homolog
PRO_0000001037

Experimental info

Mutagenesis811C → S: Loss of interaction with MUC2. Ref.12

Secondary structure

............................ 175
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95994 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: F271B1BD377BEE11

FASTA17519,979
        10         20         30         40         50         60 
MEKIPVSAFL LLVALSYTLA RDTTVKPGAK KDTKDSRPKL PQTLSRGWGD QLIWTQTYEE 

        70         80         90        100        110        120 
ALYKSKTSNK PLMIIHHLDE CPHSQALKKV FAENKEIQKL AEQFVLLNLV YETTDKHLSP 

       130        140        150        160        170 
DGQYVPRIMF VDPSLTVRAD ITGRYSNRLY AYEPADTALL LDNMKKALKL LKTEL 

« Hide

References

« Hide 'large scale' references
[1]"hAG-2, the human homologue of the Xenopus laevis cement gland gene XAG-2, is coexpressed with estrogen receptor in breast cancer cell lines."
Thompson D.A., Weigel R.J.
Biochem. Biophys. Res. Commun. 251:111-116(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Mammary gland.
[2]"Identification of human homolog of XAG-2 over-expressed in tumors."
Zhang J.S., Smith D.I.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Prostatic carcinoma.
[3]"Cloning and expression of a novel human cDNA homology to murine GOB-4 mRNA."
Fan Y.X., Yu L., Zhang X.N., Wan W.C., Wang X.K., Zhao S.Y.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[8]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-35.
[9]"hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan."
Fletcher G.C., Patel S., Tyson K., Adam P.J., Schenker M., Loader J.A., Daviet L., Legrain P., Parekh R., Harris A.L., Terrett J.A.
Br. J. Cancer 88:579-585(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LYPD3 AND DAG1.
[10]"AGR2, an androgen-inducible secretory protein overexpressed in prostate cancer."
Zhang J.-S., Gong A., Cheville J.C., Smith D.I., Young C.Y.F.
Genes Chromosomes Cancer 43:249-259(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[11]"The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation."
Wang Z., Hao Y., Lowe A.W.
Cancer Res. 68:492-497(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"The protein disulfide isomerase AGR2 is essential for production of intestinal mucus."
Park S.-W., Zhen G., Verhaeghe C., Nakagami Y., Nguyenvu L.T., Barczak A.J., Killeen N., Erle D.J.
Proc. Natl. Acad. Sci. U.S.A. 106:6950-6955(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MUC2, MUTAGENESIS OF CYS-81.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF007791 mRNA. Translation: AAC77358.1.
AF038451 mRNA. Translation: AAC82614.1.
AF088867 mRNA. Translation: AAF22484.1.
AF115926 mRNA. Translation: AAL54870.1.
AF087879 mRNA. Translation: AAP97179.1.
AY359009 mRNA. Translation: AAQ89368.1.
BT007048 mRNA. Translation: AAP35697.1.
AC073333 Genomic DNA. Translation: AAP22354.1.
BC015503 mRNA. Translation: AAH15503.1.
CCDSCCDS5364.1.
PIRJE0350.
RefSeqNP_006399.1. NM_006408.3.
XP_005249638.1. XM_005249581.2.
UniGeneHs.530009.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LNSNMR-A/B41-175[»]
2LNTNMR-A41-175[»]
ProteinModelPortalO95994.
SMRO95994. Positions 38-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115802. 14 interactions.
DIPDIP-48825N.
IntActO95994. 7 interactions.
MINTMINT-2868509.
STRING9606.ENSP00000391490.

PTM databases

PhosphoSiteO95994.

Proteomic databases

MaxQBO95994.
PaxDbO95994.
PRIDEO95994.

Protocols and materials databases

DNASU10551.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000419304; ENSP00000391490; ENSG00000106541.
GeneID10551.
KEGGhsa:10551.
UCSCuc003str.3. human.

Organism-specific databases

CTD10551.
GeneCardsGC07M016797.
HGNCHGNC:328. AGR2.
HPAHPA007912.
MIM606358. gene.
neXtProtNX_O95994.
PharmGKBPA24625.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG78144.
HOGENOMHOG000231100.
HOVERGENHBG006516.
OrthoDBEOG7DNNX2.
PhylomeDBO95994.
TreeFamTF321449.

Gene expression databases

ArrayExpressO95994.
BgeeO95994.
CleanExHS_AGR2.
GenevestigatorO95994.

Family and domain databases

Gene3D3.40.30.10. 1 hit.
InterProIPR012336. Thioredoxin-like_fold.
[Graphical view]
SUPFAMSSF52833. SSF52833. 1 hit.
ProtoNetSearch...

Other

GeneWikiAGR2.
GenomeRNAi10551.
NextBio40021.
PROO95994.
SOURCESearch...

Entry information

Entry nameAGR2_HUMAN
AccessionPrimary (citable) accession number: O95994
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM