DNA topoisomerase 3-beta-1 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 3-beta-1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase III beta-1

Gene names

Name:	TOP3B
Synonyms:	TOP3B1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Possesses negatively supercoiled DNA relaxing activity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Tissue specificity	Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. Ref.1
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.
Sequence caution	The sequence BAA20009.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ZNF408	Q9H9D4	1	EBI-373403,EBI-347633

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95985-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95985-2) The sequence of this isoform differs from the canonical sequence as follows: 703-730: GMGCNECTHPSCQHSLSMLGIGQCVECE → GECSHSLLSTGSCSLFSVPTPALHQAGL 731-862: Missing.

Isoform 3 (identifier: O95985-3) The sequence of this isoform differs from the canonical sequence as follows: 703-707: GMGCN → VVPCV 708-862: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 862

862

DNA topoisomerase 3-beta-1

PRO_0000145192

Sites

Active site

336

1

For DNA cleavage activity By similarity

Natural variations

Alternative sequence

703 – 730

28

GMGCN…CVECE → GECSHSLLSTGSCSLFSVPT PALHQAGL in isoform 2.

VSP_006525

Alternative sequence

703 – 707

5

GMGCN → VVPCV in isoform 3.

VSP_006527

Alternative sequence

708 – 862

155

Missing in isoform 3.

VSP_006528

Alternative sequence

731 – 862

132

Missing in isoform 2.

VSP_006526

Natural variant

365

1

D → N: dbSNP rs9610728. Ref.6

VAR_052591

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 75532827856CFF8F Show »	FASTA	862	96,662
10 20 30 40 50 60 MKTVLMVAEK PSLAQSIAKI LSRGSLSSHK GLNGACSVHE YTGTFAGQPV RFKMTSVCGH 70 80 90 100 110 120 VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK FLQVEGRGCD YIVLWLDCDK 130 140 150 160 170 180 EGENICFEVL DAVLPVMNKA HGGEKTVFRA RFSSITDTDI CNAMACLGEP DHNEALSVDA 190 200 210 220 230 240 RQELDLRIGC AFTRFQTKYF QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY 250 260 270 280 290 300 WVLQAKVNTD KDRSLLLDWD RVRVFDREIA QMFLNMTKLE KEAQVEATSR KEKAKQRPLA 310 320 330 340 350 360 LNTVEMLRVA SSSLGMGPQH AMQTAERLYT QGYISYPRTE TTHYPENFDL KGSLRQQANH 370 380 390 400 410 420 PYWADTVKRL LAEGINRPRK GHDAGDHPPI TPMKSATEAE LGGDAWRLYE YITRHFIATV 430 440 450 460 470 480 SHDCKYLQST ISFRIGPELF TCSGKTVLSP GFTEVMPWQS VPLEESLPTC QRGDAFPVGE 490 500 510 520 530 540 VKMLEKQTNP PDYLTEAELI TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL 550 560 570 580 590 600 GIVLVHGYYK IDAELVLPTI RSAVEKQLNL IAQGKADYRQ VLGHTLDVFK RKFHYFVDSI 610 620 630 640 650 660 AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY TLPQNGTIKL 670 680 690 700 710 720 YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM KKGMGCNECT HPSCQHSLSM 730 740 750 760 770 780 LGIGQCVECE SGVLVLDPTS GPKWKVACNK CNVVAHCFEN AHRVRVSADT CSVCEAALLD 790 800 810 820 830 840 VDFNKAKSPL PGDETQHMGC VFCDPVFQEL VELKHAASCH PMHRGGPGRR QGRGRGRARR 850 860 PPGKPNPRRP KDKMSALAAY FV « Hide
Isoform 2. Checksum: 8CC2903C7C4304E6 Show »	FASTA	730	82,123
10 20 30 40 50 60 MKTVLMVAEK PSLAQSIAKI LSRGSLSSHK GLNGACSVHE YTGTFAGQPV RFKMTSVCGH 70 80 90 100 110 120 VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK FLQVEGRGCD YIVLWLDCDK 130 140 150 160 170 180 EGENICFEVL DAVLPVMNKA HGGEKTVFRA RFSSITDTDI CNAMACLGEP DHNEALSVDA 190 200 210 220 230 240 RQELDLRIGC AFTRFQTKYF QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY 250 260 270 280 290 300 WVLQAKVNTD KDRSLLLDWD RVRVFDREIA QMFLNMTKLE KEAQVEATSR KEKAKQRPLA 310 320 330 340 350 360 LNTVEMLRVA SSSLGMGPQH AMQTAERLYT QGYISYPRTE TTHYPENFDL KGSLRQQANH 370 380 390 400 410 420 PYWADTVKRL LAEGINRPRK GHDAGDHPPI TPMKSATEAE LGGDAWRLYE YITRHFIATV 430 440 450 460 470 480 SHDCKYLQST ISFRIGPELF TCSGKTVLSP GFTEVMPWQS VPLEESLPTC QRGDAFPVGE 490 500 510 520 530 540 VKMLEKQTNP PDYLTEAELI TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL 550 560 570 580 590 600 GIVLVHGYYK IDAELVLPTI RSAVEKQLNL IAQGKADYRQ VLGHTLDVFK RKFHYFVDSI 610 620 630 640 650 660 AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY TLPQNGTIKL 670 680 690 700 710 720 YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM KKGECSHSLL STGSCSLFSV 730 PTPALHQAGL « Hide
Isoform 3. Checksum: BE43F9D46B50AA85 Show »	FASTA	707	79,839
10 20 30 40 50 60 MKTVLMVAEK PSLAQSIAKI LSRGSLSSHK GLNGACSVHE YTGTFAGQPV RFKMTSVCGH 70 80 90 100 110 120 VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK FLQVEGRGCD YIVLWLDCDK 130 140 150 160 170 180 EGENICFEVL DAVLPVMNKA HGGEKTVFRA RFSSITDTDI CNAMACLGEP DHNEALSVDA 190 200 210 220 230 240 RQELDLRIGC AFTRFQTKYF QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY 250 260 270 280 290 300 WVLQAKVNTD KDRSLLLDWD RVRVFDREIA QMFLNMTKLE KEAQVEATSR KEKAKQRPLA 310 320 330 340 350 360 LNTVEMLRVA SSSLGMGPQH AMQTAERLYT QGYISYPRTE TTHYPENFDL KGSLRQQANH 370 380 390 400 410 420 PYWADTVKRL LAEGINRPRK GHDAGDHPPI TPMKSATEAE LGGDAWRLYE YITRHFIATV 430 440 450 460 470 480 SHDCKYLQST ISFRIGPELF TCSGKTVLSP GFTEVMPWQS VPLEESLPTC QRGDAFPVGE 490 500 510 520 530 540 VKMLEKQTNP PDYLTEAELI TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL 550 560 570 580 590 600 GIVLVHGYYK IDAELVLPTI RSAVEKQLNL IAQGKADYRQ VLGHTLDVFK RKFHYFVDSI 610 620 630 640 650 660 AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY TLPQNGTIKL 670 680 690 700 YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM KKVVPCV « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new human topoisomerase III that interacts with SGS1 protein." Ng S.-W., Liu Y., Hasselblatt K.T., Mok S.C., Berkowitz R.S. Nucleic Acids Res. 27:993-1000(1999) [PubMed: 9927731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[2]	"One-megabase sequence analysis of the human immunoglobulin lambda gene locus." Kawasaki K., Minoshima S., Nakato E., Shibuya K., Shintani A., Schmeits J.L., Wang J., Shimizu N. Genome Res. 7:250-261(1997) [PubMed: 9074928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Hanai R., Li W., Wang J.C. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	Riou J.F., Goulaouic H., Grondard L. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASN-365. Tissue: Skin.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF053082 mRNA. Translation: AAD15791.1. D87012 Genomic DNA. Translation: BAA20009.1. Sequence problems. AF017146 mRNA. Translation: AAD01614.1. AF125216 mRNA. Translation: AAD29670.1. CR456596 mRNA. Translation: CAG30482.1. BC002432 mRNA. Translation: AAH02432.1.
IPI	IPI00216815. IPI00216816. IPI00292671.
RefSeq	NP_003926.1.
UniGene	Hs.436401
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95985. 3 interactions.
Proteomic databases
PRIDE	O95985.
Genome annotation databases
Ensembl	ENSG00000100038. Homo sapiens. [Contig view]
GeneID	8940.
KEGG	hsa:8940.
Organism-specific databases
GeneCards	GC22M020635.
H-InvDB	HIX0016284.
HGNC	HGNC:11993. TOP3B.
HPA	CAB009198.
MIM	603582. gene.
PharmGKB	PA36674.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O95985.
HOVERGEN	O95985.
OMA	O95985. ICNAMAC.
Enzyme and pathway databases
BRENDA	5.99.1.2. 247.
Gene expression databases
ArrayExpress	O95985.
Bgee	O95985.
CleanEx	HS_TOP3B.
GermOnline	ENSG00000100038. Homo sapiens.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR006171. Toprim_domain. IPR006154. Toprim_sub. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33622.
SOURCE	Search...

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Entry information

Entry name

TOP3B_HUMAN

Accession

Primary (citable) accession number: O95985
Secondary accession number(s): A0M8Q3, Q9BUP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents