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Protein

Reversion-inducing cysteine-rich protein with Kazal motifs

Gene

RECK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: MGI
  • metalloendopeptidase inhibitor activity Source: ProtInc
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.037.

Names & Taxonomyi

Protein namesi
Recommended name:
Reversion-inducing cysteine-rich protein with Kazal motifs
Short name:
hRECK
Alternative name(s):
Suppressor of tumorigenicity 15 protein
Gene namesi
Name:RECK
Synonyms:ST15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:11345. RECK.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • membrane Source: ProtInc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

PharmGKBiPA34314.

Polymorphism and mutation databases

BioMutaiRECK.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 942920Reversion-inducing cysteine-rich protein with Kazal motifsPRO_0000016583Add
BLAST
Propeptidei943 – 97129Removed in mature formSequence analysisPRO_0000016584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis
Disulfide bondi633 ↔ 658PROSITE-ProRule annotation
Disulfide bondi635 ↔ 654PROSITE-ProRule annotation
Disulfide bondi643 ↔ 677PROSITE-ProRule annotation
Lipidationi942 – 9421GPI-anchor amidated serineSequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO95980.
PaxDbiO95980.
PeptideAtlasiO95980.
PRIDEiO95980.

PTM databases

PhosphoSiteiO95980.

Expressioni

Tissue specificityi

Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.

Gene expression databases

BgeeiO95980.
CleanExiHS_RECK.
GenevisibleiO95980. HS.

Organism-specific databases

HPAiCAB025109.

Interactioni

Subunit structurei

Interacts with MMP-9.

Binary interactionsi

WithEntry#Exp.IntActNotes
ERBB2P046264EBI-2823742,EBI-641062

Protein-protein interaction databases

BioGridi114014. 15 interactions.
IntActiO95980. 4 interactions.
MINTiMINT-6772419.
STRINGi9606.ENSP00000367202.

Structurei

3D structure databases

ProteinModelPortaliO95980.
SMRiO95980. Positions 634-661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 8448Knot 1Add
BLAST
Repeati104 – 14138Knot 2Add
BLAST
Repeati151 – 19747Knot 3Add
BLAST
Repeati216 – 26348Knot 4Add
BLAST
Repeati292 – 33847Knot 5Add
BLAST
Domaini627 – 67347Kazal-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini698 – 75255Kazal-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini753 – 78937Kazal-like 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 3383025 X Knot repeatsAdd
BLAST

Sequence similaritiesi

Contains 3 Kazal-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiO95980.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG7X0VGC.
PhylomeDBiO95980.
TreeFamiTF324424.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVRASLRG ALLLLLAVAG VAEVAGGLAP GSAGALCCNH SKDNQMCRDV
60 70 80 90 100
CEQIFSSKSE SRLKHLLQRA PDYCPETMVE IWNCMNSSLP GVFKKSDGWV
110 120 130 140 150
GLGCCELAIA LECRQACKQA SSKNDISKVC RKEYENALFS CISRNEMGSV
160 170 180 190 200
CCSYAGHHTN CREYCQAIFR TDSSPGPSQI KAVENYCASI SPQLIHCVNN
210 220 230 240 250
YTQSYPMRNP TDSLYCCDRA EDHACQNACK RILMSKKTEM EIVDGLIEGC
260 270 280 290 300
KTQPLPQDPL WQCFLESSQS VHPGVTVHPP PSTGLDGAKL HCCSKANTST
310 320 330 340 350
CRELCTKLYS MSWGNTQSWQ EFDRFCEYNP VEVSMLTCLA DVREPCQLGC
360 370 380 390 400
RNLTYCTNFN NRPTELFRSC NAQSDQGAMN DMKLWEKGSI KMPFINIPVL
410 420 430 440 450
DIKKCQPEMW KAIACSLQIK PCHSKSRGSI ICKSDCVEIL KKCGDQNKFP
460 470 480 490 500
EDHTAESICE LLSPTDDLKN CIPLDTYLRP STLGNIVEEV THPCNPNPCP
510 520 530 540 550
ANELCEVNRK GCPSGDPCLP YFCVQGCKLG EASDFIVRQG TLIQVPSSAG
560 570 580 590 600
EVGCYKICSC GQSGLLENCM EMHCIDLQKS CIVGGKRKSH GTSFSIDCNV
610 620 630 640 650
CSCFAGNLVC STRLCLSEHS SEDDRRTFTG LPCNCADQFV PVCGQNGRTY
660 670 680 690 700
PSACIARCVG LQDHQFEFGS CMSKDPCNPN PCQKNQRCIP KPQVCLTTFD
710 720 730 740 750
KFGCSQYECV PRQLACDQVQ DPVCDTDHME HNNLCTLYQR GKSLSYKGPC
760 770 780 790 800
QPFCRATEPV CGHNGETYSS VCAAYSDRVA VDYYGDCQAV GVLSEHSSVA
810 820 830 840 850
ECASVKCPSL LAAGCKPIIP PGACCPLCAG MLRVLFDKEK LDTIAKVTNK
860 870 880 890 900
KPITVLEILQ KIRMHVSVPQ CDVFGYFSIE SEIVILIIPV DHYPKALQIE
910 920 930 940 950
ACNKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSV PSAGVRARPS
960 970
CHSLLLPLSL GLALHLLWTY N
Length:971
Mass (Da):106,457
Last modified:May 1, 1999 - v1
Checksum:i173D47D6AEE6F834
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti275 – 2751V → I.
Corresponds to variant rs16932912 [ dbSNP | Ensembl ].
VAR_034021

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50406 mRNA. Translation: BAA34060.1.
AL158830, AL138834 Genomic DNA. Translation: CAD13384.2.
AL138834, AL158830 Genomic DNA. Translation: CAH70155.1.
CH471071 Genomic DNA. Translation: EAW58318.1.
BC137093 mRNA. Translation: AAI37094.1.
CCDSiCCDS6597.1.
RefSeqiNP_066934.1. NM_021111.2.
UniGeneiHs.388918.

Genome annotation databases

EnsembliENST00000377966; ENSP00000367202; ENSG00000122707.
GeneIDi8434.
KEGGihsa:8434.
UCSCiuc003zyv.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50406 mRNA. Translation: BAA34060.1.
AL158830, AL138834 Genomic DNA. Translation: CAD13384.2.
AL138834, AL158830 Genomic DNA. Translation: CAH70155.1.
CH471071 Genomic DNA. Translation: EAW58318.1.
BC137093 mRNA. Translation: AAI37094.1.
CCDSiCCDS6597.1.
RefSeqiNP_066934.1. NM_021111.2.
UniGeneiHs.388918.

3D structure databases

ProteinModelPortaliO95980.
SMRiO95980. Positions 634-661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114014. 15 interactions.
IntActiO95980. 4 interactions.
MINTiMINT-6772419.
STRINGi9606.ENSP00000367202.

Protein family/group databases

MEROPSiI01.037.

PTM databases

PhosphoSiteiO95980.

Polymorphism and mutation databases

BioMutaiRECK.

Proteomic databases

MaxQBiO95980.
PaxDbiO95980.
PeptideAtlasiO95980.
PRIDEiO95980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377966; ENSP00000367202; ENSG00000122707.
GeneIDi8434.
KEGGihsa:8434.
UCSCiuc003zyv.4. human.

Organism-specific databases

CTDi8434.
GeneCardsiRECK.
HGNCiHGNC:11345. RECK.
HPAiCAB025109.
MIMi605227. gene.
neXtProtiNX_O95980.
PharmGKBiPA34314.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00390000018540.
HOGENOMiHOG000007167.
HOVERGENiHBG036100.
InParanoidiO95980.
KOiK17461.
OMAiVNRKGCP.
OrthoDBiEOG7X0VGC.
PhylomeDBiO95980.
TreeFamiTF324424.

Miscellaneous databases

ChiTaRSiRECK. human.
GeneWikiiRECK.
GenomeRNAii8434.
NextBioi31554.
PROiO95980.
SOURCEiSearch...

Gene expression databases

BgeeiO95980.
CleanExiHS_RECK.
GenevisibleiO95980. HS.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF07648. Kazal_2. 3 hits.
[Graphical view]
SMARTiSM00280. KAZAL. 3 hits.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of matrix metalloproteinase-9 and inhibition of tumor invasion by the membrane-anchored glycoprotein RECK."
    Takahashi C., Sheng Z., Horan T.P., Kitayama H., Maki M., Hitomi K., Kitaura Y., Takai S., Sasahara R.M., Horimoto A., Ikawa Y., Ratzkin B.J., Arakawa T., Noda M.
    Proc. Natl. Acad. Sci. U.S.A. 95:13221-13226(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION.
    Tissue: Fibroblast.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiRECK_HUMAN
AccessioniPrimary (citable) accession number: O95980
Secondary accession number(s): B2RNS1, Q5W0K6, Q8WX37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: May 1, 1999
Last modified: April 13, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.