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O95977 (S1PR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sphingosine 1-phosphate receptor 4

Short name=S1P receptor 4
Short name=S1P4
Alternative name(s):
Endothelial differentiation G-protein coupled receptor 6
Sphingosine 1-phosphate receptor Edg-6
Short name=S1P receptor Edg-6
Gene names
Name:S1PR4
Synonyms:EDG6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Sphingosine 1-phosphate receptor 4
PRO_0000069431

Regions

Topological domain1 – 5050Extracellular By similarity
Transmembrane51 – 7121Helical; Name=1; By similarity
Topological domain72 – 8413Cytoplasmic By similarity
Transmembrane85 – 10521Helical; Name=2; By similarity
Topological domain106 – 11712Extracellular By similarity
Transmembrane118 – 13821Helical; Name=3; By similarity
Topological domain139 – 16123Cytoplasmic By similarity
Transmembrane162 – 18221Helical; Name=4; By similarity
Topological domain183 – 20624Extracellular By similarity
Transmembrane207 – 22721Helical; Name=5; By similarity
Topological domain228 – 25225Cytoplasmic By similarity
Transmembrane253 – 27321Helical; Name=6; By similarity
Topological domain274 – 28815Extracellular By similarity
Transmembrane289 – 30921Helical; Name=7; By similarity
Topological domain310 – 38475Cytoplasmic By similarity

Amino acid modifications

Lipidation3231S-palmitoyl cysteine By similarity
Glycosylation21N-linked (GlcNAc...) Potential
Glycosylation301N-linked (GlcNAc...) Potential

Natural variations

Natural variant3651R → L.
Corresponds to variant rs3746072 [ dbSNP | Ensembl ].
VAR_022066

Secondary structure

..... 384
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95977 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 369A7BC56AB46A47

FASTA38441,623
        10         20         30         40         50         60 
MNATGTPVAP ESCQQLAAGG HSRLIVLHYN HSGRLAGRGG PEDGGLGALR GLSVAASCLV 

        70         80         90        100        110        120 
VLENLLVLAA ITSHMRSRRW VYYCLVNITL SDLLTGAAYL ANVLLSGART FRLAPAQWFL 

       130        140        150        160        170        180 
REGLLFTALA ASTFSLLFTA GERFATMVRP VAESGATKTS RVYGFIGLCW LLAALLGMLP 

       190        200        210        220        230        240 
LLGWNCLCAF DRCSSLLPLY SKRYILFCLV IFAGVLATIM GLYGAIFRLV QASGQKAPRP 

       250        260        270        280        290        300 
AARRKARRLL KTVLMILLAF LVCWGPLFGL LLADVFGSNL WAQEYLRGMD WILALAVLNS 

       310        320        330        340        350        360 
AVNPIIYSFR SREVCRAVLS FLCCGCLRLG MRGPGDCLAR AVEAHSGAST TDSSLRPRDS 

       370        380 
FRGSRSLSFR MREPLSSISS VRSI 

« Hide

References

« Hide 'large scale' references
[1]"EDG6, a novel G protein-coupled receptor related to receptors for bioactive lysophospholipids, is specifically expressed in lymphoid tissue."
Graeler M.H., Bernhardt G., Lipp M.
Genomics 53:164-169(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Dendritic cell.
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Blood.
[5]"Edg-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway."
Yamazaki Y., Kon J., Sato K., Tomura H., Sato M., Yoneya T., Okazaki H., Okajima F., Ohta H.
Biochem. Biophys. Res. Commun. 268:583-589(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6."
Van Brocklyn J.R., Graeler M.H., Bernhardt G., Hobson J.P., Lipp M., Spiegel S.
Blood 95:2624-2629(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Peptide design and structural characterization of a GPCR loop mimetic."
Pham T.-C.T., Kriwacki R.W., Parrill A.L.
Biopolymers 86:298-310(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 105-122.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ000479 mRNA. Translation: CAA04118.1.
AY322537 Genomic DNA. Translation: AAP84350.1.
CH471139 Genomic DNA. Translation: EAW69336.1.
CH471139 Genomic DNA. Translation: EAW69337.1.
BC014970 mRNA. Translation: AAH14970.1.
CCDSCCDS12105.1.
RefSeqNP_003766.1. NM_003775.3.
UniGeneHs.662006.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DCONMR-A105-118[»]
ProteinModelPortalO95977.
SMRO95977. Positions 24-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114243. 2 interactions.
STRING9606.ENSP00000246115.

Chemistry

BindingDBO95977.
ChEMBLCHEMBL2363041.
GuidetoPHARMACOLOGY278.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteO95977.

Proteomic databases

PaxDbO95977.
PRIDEO95977.

Protocols and materials databases

DNASU8698.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000246115; ENSP00000246115; ENSG00000125910.
GeneID8698.
KEGGhsa:8698.
UCSCuc002lxg.3. human.

Organism-specific databases

CTD8698.
GeneCardsGC19P003178.
H-InvDBHIX0174310.
HGNCHGNC:3170. S1PR4.
HPAHPA060601.
MIM603751. gene.
neXtProtNX_O95977.
PharmGKBPA162402371.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150569.
HOGENOMHOG000233501.
HOVERGENHBG103071.
InParanoidO95977.
KOK04293.
OMARANGQKA.
OrthoDBEOG708W0B.
PhylomeDBO95977.
TreeFamTF330052.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeO95977.
CleanExHS_S1PR4.
GenevestigatorO95977.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR004064. EDG6_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PANTHERPTHR22750:SF13. PTHR22750:SF13. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01526. EDG6RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95977.
GeneWikiS1PR4.
GenomeRNAi8698.
NextBio32617.
PROO95977.
SOURCESearch...

Entry information

Entry nameS1PR4_HUMAN
AccessionPrimary (citable) accession number: O95977
Secondary accession number(s): D6W612
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries