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O95977

- S1PR4_HUMAN

UniProt

O95977 - S1PR4_HUMAN

Protein

Sphingosine 1-phosphate receptor 4

Gene

S1PR4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes.2 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: ProtInc
    2. lipid binding Source: ProtInc
    3. sphingosine-1-phosphate receptor activity Source: InterPro

    GO - Biological processi

    1. activation of phospholipase C activity Source: ProtInc
    2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: Ensembl
    3. G-protein coupled receptor signaling pathway Source: ProtInc
    4. immune response Source: ProtInc
    5. positive regulation of cytosolic calcium ion concentration Source: ProtInc

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_18365. Lysosphingolipid and LPA receptors.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sphingosine 1-phosphate receptor 4
    Short name:
    S1P receptor 4
    Short name:
    S1P4
    Alternative name(s):
    Endothelial differentiation G-protein coupled receptor 6
    Sphingosine 1-phosphate receptor Edg-6
    Short name:
    S1P receptor Edg-6
    Gene namesi
    Name:S1PR4
    Synonyms:EDG6
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:3170. S1PR4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162402371.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384Sphingosine 1-phosphate receptor 4PRO_0000069431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
    Lipidationi323 – 3231S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiO95977.
    PRIDEiO95977.

    PTM databases

    PhosphoSiteiO95977.

    Expressioni

    Tissue specificityi

    Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.

    Gene expression databases

    BgeeiO95977.
    CleanExiHS_S1PR4.
    GenevestigatoriO95977.

    Organism-specific databases

    HPAiHPA060601.

    Interactioni

    Protein-protein interaction databases

    BioGridi114243. 2 interactions.
    STRINGi9606.ENSP00000246115.

    Structurei

    Secondary structure

    1
    384
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi108 – 1114
    Beta strandi113 – 1164

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DCONMR-A105-118[»]
    ProteinModelPortaliO95977.
    SMRiO95977. Positions 24-319.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95977.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5050ExtracellularBy similarityAdd
    BLAST
    Topological domaini72 – 8413CytoplasmicBy similarityAdd
    BLAST
    Topological domaini106 – 11712ExtracellularBy similarityAdd
    BLAST
    Topological domaini139 – 16123CytoplasmicBy similarityAdd
    BLAST
    Topological domaini183 – 20624ExtracellularBy similarityAdd
    BLAST
    Topological domaini228 – 25225CytoplasmicBy similarityAdd
    BLAST
    Topological domaini274 – 28815ExtracellularBy similarityAdd
    BLAST
    Topological domaini310 – 38475CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei51 – 7121Helical; Name=1By similarityAdd
    BLAST
    Transmembranei85 – 10521Helical; Name=2By similarityAdd
    BLAST
    Transmembranei118 – 13821Helical; Name=3By similarityAdd
    BLAST
    Transmembranei162 – 18221Helical; Name=4By similarityAdd
    BLAST
    Transmembranei207 – 22721Helical; Name=5By similarityAdd
    BLAST
    Transmembranei253 – 27321Helical; Name=6By similarityAdd
    BLAST
    Transmembranei289 – 30921Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG150569.
    HOGENOMiHOG000233501.
    HOVERGENiHBG103071.
    InParanoidiO95977.
    KOiK04293.
    OMAiRANGQKA.
    OrthoDBiEOG708W0B.
    PhylomeDBiO95977.
    TreeFamiTF330052.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR004064. EDG6_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view]
    PANTHERiPTHR22750:SF13. PTHR22750:SF13. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01526. EDG6RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O95977-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNATGTPVAP ESCQQLAAGG HSRLIVLHYN HSGRLAGRGG PEDGGLGALR    50
    GLSVAASCLV VLENLLVLAA ITSHMRSRRW VYYCLVNITL SDLLTGAAYL 100
    ANVLLSGART FRLAPAQWFL REGLLFTALA ASTFSLLFTA GERFATMVRP 150
    VAESGATKTS RVYGFIGLCW LLAALLGMLP LLGWNCLCAF DRCSSLLPLY 200
    SKRYILFCLV IFAGVLATIM GLYGAIFRLV QASGQKAPRP AARRKARRLL 250
    KTVLMILLAF LVCWGPLFGL LLADVFGSNL WAQEYLRGMD WILALAVLNS 300
    AVNPIIYSFR SREVCRAVLS FLCCGCLRLG MRGPGDCLAR AVEAHSGAST 350
    TDSSLRPRDS FRGSRSLSFR MREPLSSISS VRSI 384
    Length:384
    Mass (Da):41,623
    Last modified:May 1, 1999 - v1
    Checksum:i369A7BC56AB46A47
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti365 – 3651R → L.
    Corresponds to variant rs3746072 [ dbSNP | Ensembl ].
    VAR_022066

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000479 mRNA. Translation: CAA04118.1.
    AY322537 Genomic DNA. Translation: AAP84350.1.
    CH471139 Genomic DNA. Translation: EAW69336.1.
    CH471139 Genomic DNA. Translation: EAW69337.1.
    BC014970 mRNA. Translation: AAH14970.1.
    CCDSiCCDS12105.1.
    RefSeqiNP_003766.1. NM_003775.3.
    UniGeneiHs.662006.

    Genome annotation databases

    EnsembliENST00000246115; ENSP00000246115; ENSG00000125910.
    GeneIDi8698.
    KEGGihsa:8698.
    UCSCiuc002lxg.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000479 mRNA. Translation: CAA04118.1 .
    AY322537 Genomic DNA. Translation: AAP84350.1 .
    CH471139 Genomic DNA. Translation: EAW69336.1 .
    CH471139 Genomic DNA. Translation: EAW69337.1 .
    BC014970 mRNA. Translation: AAH14970.1 .
    CCDSi CCDS12105.1.
    RefSeqi NP_003766.1. NM_003775.3.
    UniGenei Hs.662006.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DCO NMR - A 105-118 [» ]
    ProteinModelPortali O95977.
    SMRi O95977. Positions 24-319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114243. 2 interactions.
    STRINGi 9606.ENSP00000246115.

    Chemistry

    BindingDBi O95977.
    ChEMBLi CHEMBL2363041.
    GuidetoPHARMACOLOGYi 278.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei O95977.

    Proteomic databases

    PaxDbi O95977.
    PRIDEi O95977.

    Protocols and materials databases

    DNASUi 8698.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000246115 ; ENSP00000246115 ; ENSG00000125910 .
    GeneIDi 8698.
    KEGGi hsa:8698.
    UCSCi uc002lxg.3. human.

    Organism-specific databases

    CTDi 8698.
    GeneCardsi GC19P003178.
    H-InvDB HIX0174310.
    HGNCi HGNC:3170. S1PR4.
    HPAi HPA060601.
    MIMi 603751. gene.
    neXtProti NX_O95977.
    PharmGKBi PA162402371.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG150569.
    HOGENOMi HOG000233501.
    HOVERGENi HBG103071.
    InParanoidi O95977.
    KOi K04293.
    OMAi RANGQKA.
    OrthoDBi EOG708W0B.
    PhylomeDBi O95977.
    TreeFami TF330052.

    Enzyme and pathway databases

    Reactomei REACT_18365. Lysosphingolipid and LPA receptors.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    EvolutionaryTracei O95977.
    GeneWikii S1PR4.
    GenomeRNAii 8698.
    NextBioi 32617.
    PROi O95977.
    SOURCEi Search...

    Gene expression databases

    Bgeei O95977.
    CleanExi HS_S1PR4.
    Genevestigatori O95977.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR004064. EDG6_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR004061. S1P_rcpt.
    [Graphical view ]
    PANTHERi PTHR22750:SF13. PTHR22750:SF13. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01526. EDG6RECEPTOR.
    PR00237. GPCRRHODOPSN.
    PR01523. S1PRECEPTOR.
    PROSITEi PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "EDG6, a novel G protein-coupled receptor related to receptors for bioactive lysophospholipids, is specifically expressed in lymphoid tissue."
      Graeler M.H., Bernhardt G., Lipp M.
      Genomics 53:164-169(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Dendritic cell.
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Kopatz S.A., Aronstam R.S., Sharma S.V.
      Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Blood.
    5. "Edg-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway."
      Yamazaki Y., Kon J., Sato K., Tomura H., Sato M., Yoneya T., Okazaki H., Okajima F., Ohta H.
      Biochem. Biophys. Res. Commun. 268:583-589(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6."
      Van Brocklyn J.R., Graeler M.H., Bernhardt G., Hobson J.P., Lipp M., Spiegel S.
      Blood 95:2624-2629(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Peptide design and structural characterization of a GPCR loop mimetic."
      Pham T.-C.T., Kriwacki R.W., Parrill A.L.
      Biopolymers 86:298-310(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 105-122.

    Entry informationi

    Entry nameiS1PR4_HUMAN
    AccessioniPrimary (citable) accession number: O95977
    Secondary accession number(s): D6W612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3