Sphingosine 1-phosphate receptor 4 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot O95977 (S1PR4_HUMAN)

Last modified June 16, 2009. Version 75. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Sphingosine 1-phosphate receptor 4
      Short name=S1P receptor 4
      Short name=S1P4
Alternative name(s):
    Sphingosine 1-phosphate receptor Edg-6
      Short name=S1P receptor Edg-6
    Endothelial differentiation G-protein coupled receptor 6

Gene names

Name:	S1PR4
Synonyms:	EDG6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure
Gene Ontology (GO)
Biological process	activation of phospholipase C activity Ref.4 Traceable author statement. Source: ProtInc elevation of cytosolic calcium ion concentration Ref.4 Traceable author statement. Source: ProtInc immune response Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	lipid binding Ref.4 Traceable author statement. Source: ProtInc lysosphingolipid and lysophosphatidic acid receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 384

384

Sphingosine 1-phosphate receptor 4

PRO_0000069431

Regions

Topological domain

1 – 50

Extracellular By similarity

Transmembrane

51 – 71

1 By similarity

Topological domain

72 – 84

Cytoplasmic By similarity

Transmembrane

85 – 105

2 By similarity

Topological domain

106 – 117

Extracellular By similarity

Transmembrane

118 – 138

3 By similarity

Topological domain

139 – 161

Cytoplasmic By similarity

Transmembrane

162 – 182

4 By similarity

Topological domain

183 – 206

Extracellular By similarity

Transmembrane

207 – 227

5 By similarity

Topological domain

228 – 252

Cytoplasmic By similarity

Transmembrane

253 – 273

6 By similarity

Topological domain

274 – 288

Extracellular By similarity

Transmembrane

289 – 309

7 By similarity

Topological domain

310 – 384

Cytoplasmic By similarity

Amino acid modifications

Lipidation

323

S-palmitoyl cysteine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

365

R → L: dbSNP rs3746072.

VAR_022066

Secondary structure

384

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

O95977-1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 369A7BC56AB46A47
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FASTA

384

41,623

        10         20         30         40         50         60 
MNATGTPVAP ESCQQLAAGG HSRLIVLHYN HSGRLAGRGG PEDGGLGALR GLSVAASCLV 

        70         80         90        100        110        120 
VLENLLVLAA ITSHMRSRRW VYYCLVNITL SDLLTGAAYL ANVLLSGART FRLAPAQWFL 

       130        140        150        160        170        180 
REGLLFTALA ASTFSLLFTA GERFATMVRP VAESGATKTS RVYGFIGLCW LLAALLGMLP 

       190        200        210        220        230        240 
LLGWNCLCAF DRCSSLLPLY SKRYILFCLV IFAGVLATIM GLYGAIFRLV QASGQKAPRP 

       250        260        270        280        290        300 
AARRKARRLL KTVLMILLAF LVCWGPLFGL LLADVFGSNL WAQEYLRGMD WILALAVLNS 

       310        320        330        340        350        360 
AVNPIIYSFR SREVCRAVLS FLCCGCLRLG MRGPGDCLAR AVEAHSGAST TDSSLRPRDS 

       370        380 
FRGSRSLSFR MREPLSSISS VRSI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EDG6, a novel G protein-coupled receptor related to receptors for bioactive lysophospholipids, is specifically expressed in lymphoid tissue." Graeler M.H., Bernhardt G., Lipp M. Genomics 53:164-169(1998) [PubMed: 9790765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Dendritic cell.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
[4]	"Edg-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway." Yamazaki Y., Kon J., Sato K., Tomura H., Sato M., Yoneya T., Okazaki H., Okajima F., Ohta H. Biochem. Biophys. Res. Commun. 268:583-589(2000) [PubMed: 10679247] [Abstract] Cited for: FUNCTION.
[5]	"Sphingosine-1-phosphate is a ligand for the G protein-coupled receptor EDG-6." Van Brocklyn J.R., Graeler M.H., Bernhardt G., Hobson J.P., Lipp M., Spiegel S. Blood 95:2624-2629(2000) [PubMed: 10753843] [Abstract] Cited for: FUNCTION.
[6]	"Peptide design and structural characterization of a GPCR loop mimetic." Pham T.-C.T., Kriwacki R.W., Parrill A.L. Biopolymers 86:298-310(2007) [PubMed: 17443712] [Abstract] Cited for: STRUCTURE BY NMR OF 105-122.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

AJ000479 mRNA. Translation: CAA04118.1.
AY322537 Genomic DNA. Translation: AAP84350.1.
BC014970 mRNA. Translation: AAH14970.1.

IPI

IPI00021534.

RefSeq

NP_003766.1.

UniGene

Hs.662006

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DCO	NMR	-	A	105-122	[»]

ModBase

Search...

Protein family/group databases

GPCRDB

Search...

Proteomic databases

PRIDE

O95977.

Genome annotation databases

Ensembl

ENSG00000125910. Homo sapiens. [Contig view]

GeneID

8698.

KEGG

hsa:8698.

Organism-specific databases

GeneCards

GC19P003130.

H-InvDB

HIX0014635.

HGNC

HGNC:3170. S1PR4.

MIM

603751. gene.

PharmGKB

PA27610.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O95977.

HOVERGEN

O95977.

OMA

O95977. AFVVCWG.

Enzyme and pathway databases

Pathway_Interaction_DB

s1p_s1p4_pathway. S1P4 pathway.
s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway.

Gene expression databases

ArrayExpress

O95977.

Bgee

O95977.

CleanEx

HS_S1PR4.

GermOnline

ENSG00000125910. Homo sapiens.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR004064. EDG6_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
IPR004061. S1P_rcpt.
[Graphical view]

PANTHER

PTHR22750:SF13. EDG6_rcpt. 1 hit.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR01526. EDG6RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. False negative.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

32617.

SOURCE

Search...

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Entry information

Entry name

S1PR4_HUMAN

Accession

Primary (citable) accession number: O95977

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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