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Protein

Probable tRNA pseudouridine synthase 2

Gene

TRUB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.By similarity

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981NucleophileBy similarity

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • pseudouridine synthase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase 2 (EC:5.4.99.-)
Gene namesi
Name:TRUB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:17170. TRUB2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38209.

Polymorphism and mutation databases

BioMutaiTRUB2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Probable tRNA pseudouridine synthase 2PRO_0000252090Add
BLAST

Proteomic databases

EPDiO95900.
MaxQBiO95900.
PaxDbiO95900.
PRIDEiO95900.

PTM databases

iPTMnetiO95900.
PhosphoSiteiO95900.

Expressioni

Gene expression databases

BgeeiO95900.
CleanExiHS_TRUB2.
ExpressionAtlasiO95900. baseline and differential.
GenevisibleiO95900. HS.

Organism-specific databases

HPAiHPA021211.
HPA052190.

Interactioni

Protein-protein interaction databases

BioGridi117942. 28 interactions.
IntActiO95900. 5 interactions.
MINTiMINT-8052648.
STRINGi9606.ENSP00000361982.

Structurei

3D structure databases

ProteinModelPortaliO95900.
SMRiO95900. Positions 90-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

eggNOGiKOG2559. Eukaryota.
ENOG4110V1W. LUCA.
GeneTreeiENSGT00530000063611.
HOGENOMiHOG000231821.
HOVERGENiHBG053337.
InParanoidiO95900.
OMAiHGCRLLT.
OrthoDBiEOG7RFTHX.
PhylomeDBiO95900.
TreeFamiTF320759.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
[Graphical view]
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95900-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSAGLSRLH GLFAVYKPPG LKWKHLRDTV ELQLLKGLNA RKPPAPKQRV
60 70 80 90 100
RFLLGPMEGS EEKELTLTAT SVPSFINHPL VCGPAFAHLK VGVGHRLDAQ
110 120 130 140 150
ASGVLVLGVG HGCRLLTDMY NAHLTKDYTV RGLLGKATDD FREDGRLVEK
160 170 180 190 200
TTYDHVTREK LDRILAVIQG SHQKALVMYS NLDLKTQEAY EMAVRGLIRP
210 220 230 240 250
MNKSPMLITG IRCLYFAPPE FLLEVQCMHE TQKELRKLVH EIGLELKTTA
260 270 280 290 300
VCTQVRRTRD GFFTLDSALL RTQWDLTNIQ DAIRAATPQV AAELEKSLSP
310 320 330
GLDTKQLPSP GWSWDSQGPS STLGLERGAG Q
Length:331
Mass (Da):36,694
Last modified:May 1, 1999 - v1
Checksum:i60DD5F9FEA998684
GO
Isoform 2 (identifier: O95900-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):30,454
Checksum:iF13BE676EC85FACB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti79 – 791P → L.
Corresponds to variant rs2231630 [ dbSNP | Ensembl ].
VAR_051608
Natural varianti93 – 931V → L.
Corresponds to variant rs2072394 [ dbSNP | Ensembl ].
VAR_027749
Natural varianti209 – 2091T → S.
Corresponds to variant rs2231637 [ dbSNP | Ensembl ].
VAR_051609

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5656Missing in isoform 2. 1 PublicationVSP_056094Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131848 mRNA. Translation: AAD20059.1.
AK001956 mRNA. Translation: BAA92001.1.
AK301986 mRNA. Translation: BAH13601.1.
AL359091 Genomic DNA. Translation: CAI13485.1.
BC001457 mRNA. Translation: AAH01457.1.
CCDSiCCDS6897.1. [O95900-1]
RefSeqiNP_056494.1. NM_015679.1. [O95900-1]
UniGeneiHs.632685.

Genome annotation databases

EnsembliENST00000372890; ENSP00000361982; ENSG00000167112. [O95900-1]
GeneIDi26995.
KEGGihsa:26995.
UCSCiuc004buq.2. human. [O95900-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131848 mRNA. Translation: AAD20059.1.
AK001956 mRNA. Translation: BAA92001.1.
AK301986 mRNA. Translation: BAH13601.1.
AL359091 Genomic DNA. Translation: CAI13485.1.
BC001457 mRNA. Translation: AAH01457.1.
CCDSiCCDS6897.1. [O95900-1]
RefSeqiNP_056494.1. NM_015679.1. [O95900-1]
UniGeneiHs.632685.

3D structure databases

ProteinModelPortaliO95900.
SMRiO95900. Positions 90-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117942. 28 interactions.
IntActiO95900. 5 interactions.
MINTiMINT-8052648.
STRINGi9606.ENSP00000361982.

PTM databases

iPTMnetiO95900.
PhosphoSiteiO95900.

Polymorphism and mutation databases

BioMutaiTRUB2.

Proteomic databases

EPDiO95900.
MaxQBiO95900.
PaxDbiO95900.
PRIDEiO95900.

Protocols and materials databases

DNASUi26995.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372890; ENSP00000361982; ENSG00000167112. [O95900-1]
GeneIDi26995.
KEGGihsa:26995.
UCSCiuc004buq.2. human. [O95900-1]

Organism-specific databases

CTDi26995.
GeneCardsiTRUB2.
HGNCiHGNC:17170. TRUB2.
HPAiHPA021211.
HPA052190.
MIMi610727. gene.
neXtProtiNX_O95900.
PharmGKBiPA38209.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2559. Eukaryota.
ENOG4110V1W. LUCA.
GeneTreeiENSGT00530000063611.
HOGENOMiHOG000231821.
HOVERGENiHBG053337.
InParanoidiO95900.
OMAiHGCRLLT.
OrthoDBiEOG7RFTHX.
PhylomeDBiO95900.
TreeFamiTF320759.

Miscellaneous databases

ChiTaRSiTRUB2. human.
GenomeRNAii26995.
PROiO95900.
SOURCEiSearch...

Gene expression databases

BgeeiO95900.
CleanExiHS_TRUB2.
ExpressionAtlasiO95900. baseline and differential.
GenevisibleiO95900. HS.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
[Graphical view]
PfamiPF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mei G., Yu W., Gibbs R.A.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Placenta and Testis.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  5. "The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1."
    Zucchini C., Strippoli P., Biolchi A., Solmi R., Lenzi L., D'Addabbo P., Carinci P., Valvassori L.
    Int. J. Mol. Med. 11:697-704(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRUB2_HUMAN
AccessioniPrimary (citable) accession number: O95900
Secondary accession number(s): B7Z7G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.