Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Noelin-2

Gene

OLFM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and nuclear translocation of OLFM2 where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (PubMed:25298399). Plays a role in AMPAR complex organization (By similarity).By similarity1 Publication

GO - Biological processi

  • locomotory behavior Source: Ensembl
  • positive regulation of smooth muscle cell differentiation Source: UniProtKB
  • protein secretion Source: MGI
  • visual perception Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Noelin-2
Alternative name(s):
Olfactomedin-2
Gene namesi
Name:OLFM2
Synonyms:NOE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:17189. OLFM2.

Subcellular locationi

  • Secreted 1 Publication
  • Cell junctionsynapse By similarity
  • Membrane By similarity
  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Nuclear localization is induced by TGF-beta.1 Publication

GO - Cellular componenti

  • AMPA glutamate receptor complex Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • extracellular region Source: MGI
  • nucleus Source: UniProtKB
  • synaptic membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi144 – 1441R → Q: No effect on secretion. 1 Publication
Mutagenesisi420 – 4201L → S: Completely blocks secretion. Also significantly inhibits secretion of OLFM1 and OLFM3. 1 Publication

Organism-specific databases

PharmGKBiPA31916.

Polymorphism and mutation databases

BioMutaiOLFM2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 454434Noelin-2PRO_0000020078Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence analysis
Glycosylationi155 – 1551N-linked (GlcNAc...)Sequence analysis
Disulfide bondi195 ↔ 377PROSITE-ProRule annotation
Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis
Glycosylationi399 – 3991N-linked (GlcNAc...)Sequence analysis
Glycosylationi441 – 4411N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO95897.
MaxQBiO95897.
PaxDbiO95897.
PeptideAtlasiO95897.
PRIDEiO95897.

PTM databases

iPTMnetiO95897.
PhosphoSiteiO95897.

Expressioni

Tissue specificityi

Expressed in aortic smooth muscle (at protein level).1 Publication

Inductioni

By TGF-beta.1 Publication

Gene expression databases

BgeeiO95897.
CleanExiHS_OLFM2.
ExpressionAtlasiO95897. baseline and differential.
GenevisibleiO95897. HS.

Organism-specific databases

HPAiHPA049961.
HPA057771.

Interactioni

Subunit structurei

Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including OLFM2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Interacts with GRIA2 (By similarity). Interacts with OLFM1 and OLFM3 (PubMed:21228389). Interacts with SRF; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2 (PubMed:25298399).By similarity2 Publications

Protein-protein interaction databases

BioGridi125007. 36 interactions.
IntActiO95897. 11 interactions.
MINTiMINT-1385503.
STRINGi9606.ENSP00000264833.

Structurei

3D structure databases

ProteinModelPortaliO95897.
SMRiO95897. Positions 193-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini194 – 446253Olfactomedin-likePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili58 – 8528Sequence analysisAdd
BLAST
Coiled coili136 – 19358Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 olfactomedin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiENOG410INP8. Eukaryota.
ENOG410ZRHT. LUCA.
GeneTreeiENSGT00760000119005.
HOGENOMiHOG000232069.
HOVERGENiHBG006513.
InParanoidiO95897.
OMAiFAYYTNT.
OrthoDBiEOG75F4CZ.
PhylomeDBiO95897.
TreeFamiTF315964.

Family and domain databases

InterProiIPR031219. Noelin-2.
IPR022082. Noelin_dom.
IPR003112. Olfac-like_dom.
[Graphical view]
PANTHERiPTHR23192:SF27. PTHR23192:SF27. 1 hit.
PfamiPF12308. Noelin-1. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
SMARTiSM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS51132. OLF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWPLTVPPPL LLLLCSGLAG QTLFQNPEEG WQLYTSAQAP DGKCICTAVI
60 70 80 90 100
PAQSTCSRDG RSRELRQLME KVQNVSQSME VLELRTYRDL QYVRGMETLM
110 120 130 140 150
RSLDARLRAA DGSLSAKSFQ ELKDRMTELL PLSSVLEQYK ADTRTIVRLR
160 170 180 190 200
EEVRNLSGSL AAIQEEMGAY GYEDLQQRVM ALEARLHACA QKLGCGKLTG
210 220 230 240 250
VSNPITVRAM GSRFGSWMTD TMAPSADSRV WYMDGYYKGR RVLEFRTLGD
260 270 280 290 300
FIKGQNFIQH LLPQPWAGTG HVVYNGSLFY NKYQSNVVVK YHFRSRSVLV
310 320 330 340 350
QRSLPGAGYN NTFPYSWGGF SDMDFMVDES GLWAVYTTNQ NAGNIVVSRL
360 370 380 390 400
DPHTLEVMRS WDTGYPKRSA GEAFMICGVL YVTNSHLAGA KVYFAYFTNT
410 420 430 440 450
SSYEYTDVPF HNQYSHISML DYNPRERALY TWNNGHQVLY NVTLFHVIST

SGDP
Length:454
Mass (Da):51,386
Last modified:June 7, 2005 - v2
Checksum:iEBF4AE8DF909C77F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti86 – 861T → M in a colorectal cancer sample; somatic mutation; no effect on secretion. 2 Publications
VAR_036532
Natural varianti106 – 1061R → Q.1 Publication
Corresponds to variant rs2303100 [ dbSNP | Ensembl ].
VAR_022550
Natural varianti127 – 1271T → M.1 Publication
Corresponds to variant rs11556087 [ dbSNP | Ensembl ].
VAR_050423

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131839 mRNA. Translation: AAD20056.1.
AC008742 Genomic DNA. No translation available.
BC011361 mRNA. Translation: AAH11361.1.
BK001428 Genomic DNA. Translation: DAA01550.1.
CCDSiCCDS12221.1.
RefSeqiNP_001291276.1. NM_001304347.1.
NP_001291277.1. NM_001304348.1.
NP_477512.1. NM_058164.3.
UniGeneiHs.169743.

Genome annotation databases

EnsembliENST00000264833; ENSP00000264833; ENSG00000105088.
GeneIDi93145.
KEGGihsa:93145.
UCSCiuc002mmp.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131839 mRNA. Translation: AAD20056.1.
AC008742 Genomic DNA. No translation available.
BC011361 mRNA. Translation: AAH11361.1.
BK001428 Genomic DNA. Translation: DAA01550.1.
CCDSiCCDS12221.1.
RefSeqiNP_001291276.1. NM_001304347.1.
NP_001291277.1. NM_001304348.1.
NP_477512.1. NM_058164.3.
UniGeneiHs.169743.

3D structure databases

ProteinModelPortaliO95897.
SMRiO95897. Positions 193-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125007. 36 interactions.
IntActiO95897. 11 interactions.
MINTiMINT-1385503.
STRINGi9606.ENSP00000264833.

PTM databases

iPTMnetiO95897.
PhosphoSiteiO95897.

Polymorphism and mutation databases

BioMutaiOLFM2.

Proteomic databases

EPDiO95897.
MaxQBiO95897.
PaxDbiO95897.
PeptideAtlasiO95897.
PRIDEiO95897.

Protocols and materials databases

DNASUi93145.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264833; ENSP00000264833; ENSG00000105088.
GeneIDi93145.
KEGGihsa:93145.
UCSCiuc002mmp.4. human.

Organism-specific databases

CTDi93145.
GeneCardsiOLFM2.
HGNCiHGNC:17189. OLFM2.
HPAiHPA049961.
HPA057771.
neXtProtiNX_O95897.
PharmGKBiPA31916.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INP8. Eukaryota.
ENOG410ZRHT. LUCA.
GeneTreeiENSGT00760000119005.
HOGENOMiHOG000232069.
HOVERGENiHBG006513.
InParanoidiO95897.
OMAiFAYYTNT.
OrthoDBiEOG75F4CZ.
PhylomeDBiO95897.
TreeFamiTF315964.

Miscellaneous databases

ChiTaRSiOLFM2. human.
GeneWikiiOLFM2.
GenomeRNAii93145.
PROiO95897.

Gene expression databases

BgeeiO95897.
CleanExiHS_OLFM2.
ExpressionAtlasiO95897. baseline and differential.
GenevisibleiO95897. HS.

Family and domain databases

InterProiIPR031219. Noelin-2.
IPR022082. Noelin_dom.
IPR003112. Olfac-like_dom.
[Graphical view]
PANTHERiPTHR23192:SF27. PTHR23192:SF27. 1 hit.
PfamiPF12308. Noelin-1. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
SMARTiSM00284. OLF. 1 hit.
[Graphical view]
PROSITEiPS51132. OLF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mei G., Yu W., Gibbs R.A.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-106 AND MET-127.
    Tissue: Brain.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Bioinformatic approaches for identification and characterization of olfactomedin related genes with a potential role in pathogenesis of ocular disorders."
    Mukhopadhyay A., Talukdar S., Bhattacharjee A., Ray K.
    Mol. Vis. 10:304-314(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF GENOMIC DNA.
  5. "Olfactomedin 2: expression in the eye and interaction with other olfactomedin domain-containing proteins."
    Sultana A., Nakaya N., Senatorov V.V., Tomarev S.I.
    Invest. Ophthalmol. Vis. Sci. 52:2584-2592(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH OLFM1 AND OLFM3, GLYCOSYLATION, CHARACTERIZATION OF VARIANT MET-86, MUTAGENESIS OF ARG-144 AND LEU-420.
  6. "Olfactomedin 2, a novel regulator for transforming growth factor-beta-induced smooth muscle differentiation of human embryonic stem cell-derived mesenchymal cells."
    Shi N., Guo X., Chen S.Y.
    Mol. Biol. Cell 25:4106-4114(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SRF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  7. Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-86.

Entry informationi

Entry nameiNOE2_HUMAN
AccessioniPrimary (citable) accession number: O95897
Secondary accession number(s): Q6IMJ3, Q96FC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.