ID   TXD12_HUMAN             Reviewed;         172 AA.
AC   O95881; Q5T1T4; Q96H50;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   07-JUL-2009, entry version 79.
DE   RecName: Full=Thioredoxin domain-containing protein 12;
DE            EC=1.8.4.2;
DE   AltName: Full=Thioredoxin-like protein p19;
DE   AltName: Full=Endoplasmic reticulum protein ERp19;
DE   AltName: Full=ERp18;
DE   AltName: Full=hTLP19;
DE   Flags: Precursor;
GN   Name=TXNDC12; Synonyms=TLP19; ORFNames=UNQ713/PRO1376;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   MEDLINE=22919389; PubMed=14557066; DOI=10.1016/S0378-1119(03)00732-7;
RA   Liu F., Rong Y.P., Zeng L.C., Zhang X., Han Z.G.;
RT   "Isolation and characterization of a novel human thioredoxin-like gene
RT   hTLP19 encoding a secretory protein.";
RL   Gene 315:71-78(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RA   Mei G., Yu W., Gibbs R.A.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   MEDLINE=22887296; PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA   Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA   Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA   Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA   Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA   Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA   Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale
RT   effort to identify novel human secreted and transmembrane proteins: a
RT   bioinformatics assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA   Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA   Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA   McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA   Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA   Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA   Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA   Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA   Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA   Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA   Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA   Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA   Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA   Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA   Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA   Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA   Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA   Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA   Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA   Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA   Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA   Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA   Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA   Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Colon, Kidney, and Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PROTEIN SEQUENCE OF 27-41.
RX   PubMed=15340161; DOI=10.1110/ps.04682504;
RA   Zhang Z., Henzel W.J.;
RT   "Signal peptide prediction based on analysis of experimentally
RT   verified cleavage sites.";
RL   Protein Sci. 13:2819-2824(2004).
RN   [7]
RP   FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BOND, AND MUTAGENESIS OF
RP   CYS-66 AND CYS-69.
RX   PubMed=12761212; DOI=10.1074/jbc.M304598200;
RA   Alanen H.I., Williamson R.A., Howard M.J., Lappi A.-K., Jaentti H.P.,
RA   Rautio S.M., Kellokumpu S., Ruddock L.W.;
RT   "Functional characterization of ERp18, a new endoplasmic reticulum-
RT   located thioredoxin superfamily member.";
RL   J. Biol. Chem. 278:28912-28920(2003).
RN   [8]
RP   IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RA   Colinge J., Superti-Furga G., Bennett K.L.;
RL   Submitted (OCT-2008) to UniProtKB.
CC   -!- FUNCTION: Possesses significant protein thiol-disulfide oxidase
CC       activity.
CC   -!- CATALYTIC ACTIVITY: 2 glutathione + protein-disulfide =
CC       glutathione disulfide + protein-dithiol.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=25 uM for Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn;
CC       pH dependence:
CC         Optimum pH is 6.5;
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen.
CC   -!- TISSUE SPECIFICITY: Widely expressed.
CC   -!- SIMILARITY: Contains 1 thioredoxin domain.
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DR   EMBL; AF543416; AAN34781.1; -; mRNA.
DR   EMBL; AF131758; AAD20035.1; -; mRNA.
DR   EMBL; AY358982; AAQ89341.1; -; mRNA.
DR   EMBL; AL445685; CAI17031.1; -; Genomic_DNA.
DR   EMBL; BC001493; AAH01493.1; -; mRNA.
DR   EMBL; BC008953; AAH08953.1; -; mRNA.
DR   EMBL; BC008913; AAH08913.1; -; mRNA.
DR   IPI; IPI00026328; -.
DR   RefSeq; NP_056997.1; -.
DR   UniGene; Hs.476033; -.
DR   PDB; 1SEN; X-ray; 1.20 A; A=23-172.
DR   PDBsum; 1SEN; -.
DR   SMR; O95881; 30-163.
DR   PeptideAtlas; O95881; -.
DR   PRIDE; O95881; -.
DR   Ensembl; ENSG00000117862; Homo sapiens.
DR   GeneID; 51060; -.
DR   KEGG; hsa:51060; -.
DR   UCSC; uc001cti.1; human.
DR   GeneCards; GC01M052199; -.
DR   H-InvDB; HIX0000579; -.
DR   H-InvDB; HIX0029114; -.
DR   HGNC; HGNC:24626; TXNDC12.
DR   MIM; 609448; gene.
DR   PharmGKB; PA142670665; -.
DR   HOGENOM; O95881; -.
DR   HOVERGEN; O95881; -.
DR   OMA; O95881; CHACKAL.
DR   BRENDA; 1.8.4.2; 247.
DR   DrugBank; DB00143; Glutathione.
DR   NextBio; 53641; -.
DR   ArrayExpress; O95881; -.
DR   Bgee; O95881; -.
DR   CleanEx; HS_TXNDC12; -.
DR   GermOnline; ENSG00000117862; Homo sapiens.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0019153; F:protein-disulfide reductase (glutathione) a...; IEA:EC.
DR   GO; GO:0045454; P:cell redox homeostasis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017936; Thioredoxin-like.
DR   InterPro; IPR017937; Thioredoxin_CS.
DR   InterPro; IPR012335; Thioredoxin_fold.
DR   Gene3D; G3DSA:3.40.30.10; Thioredoxin_fold; 1.
DR   PROSITE; PS00194; THIOREDOXIN_1; 1.
DR   PROSITE; PS51352; THIOREDOXIN_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   Disulfide bond; Endoplasmic reticulum; Oxidoreductase;
KW   Redox-active center; Signal.
FT   SIGNAL        1     26
FT   CHAIN        27    172       Thioredoxin domain-containing protein 12.
FT                                /FTId=PRO_0000034189.
FT   MOTIF       169    172       Prevents secretion from ER (Potential).
FT   DISULFID     66     69       Redox-active.
FT   MUTAGEN      66     66       C->S: Loss of oxidase activity.
FT   MUTAGEN      69     69       C->S: Loss of oxidase activity.
FT   CONFLICT    102    102       D -> H (in Ref. 5; AAH08913).
FT   HELIX        43     53
FT   STRAND       57     62
FT   HELIX        67     77
FT   HELIX        80     86
FT   STRAND       89     95
FT   HELIX        96     98
FT   HELIX       103    105
FT   STRAND      112    118
FT   HELIX       144    158
FT   HELIX       159    161
SQ   SEQUENCE   172 AA;  19206 MW;  3092E9515A7C4094 CRC64;
     METRPRLGAT CLLGFSFLLL VISSDGHNGL GKGFGDHIHW RTLEDGKKEA AASGLPLMVI
     IHKSWCGACK ALKPKFAEST EISELSHNFV MVNLEDEEEP KDEDFSPDGG YIPRILFLDP
     SGKVHPEIIN ENGNPSYKYF YVSAEQVVQG MKEAQERLTG DAFRKKHLED EL
//