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Protein

Thioredoxin domain-containing protein 12

Gene

TXNDC12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possesses significant protein thiol-disulfide oxidase activity.1 Publication

Catalytic activityi

2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol.

Kineticsi

  1. KM=25 µM for Asn-Arg-Cys-Ser-Gln-Gly-Ser-Cys-Trp-Asn

    pH dependencei

    Optimum pH is 6.5.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thioredoxin domain-containing protein 12 (EC:1.8.4.2)
    Alternative name(s):
    Endoplasmic reticulum resident protein 18
    Short name:
    ER protein 18
    Short name:
    ERp18
    Endoplasmic reticulum resident protein 19
    Short name:
    ER protein 19
    Short name:
    ERp19
    Thioredoxin-like protein p19
    hTLP19
    Gene namesi
    Name:TXNDC12
    Synonyms:TLP19
    ORF Names:UNQ713/PRO1376
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:24626. TXNDC12.

    Subcellular locationi

    GO - Cellular componenti

    • endoplasmic reticulum lumen Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi66C → S: Loss of oxidase activity. 1 Publication1
    Mutagenesisi69C → S: Loss of oxidase activity. 1 Publication1

    Organism-specific databases

    DisGeNETi51060.
    OpenTargetsiENSG00000117862.
    PharmGKBiPA142670665.

    Chemistry databases

    DrugBankiDB00143. Glutathione.

    Polymorphism and mutation databases

    BioMutaiTXNDC12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 261 PublicationAdd BLAST26
    ChainiPRO_000003418927 – 172Thioredoxin domain-containing protein 12Add BLAST146

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi66 ↔ 69Redox-activePROSITE-ProRule annotation2 Publications

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    EPDiO95881.
    MaxQBiO95881.
    PaxDbiO95881.
    PeptideAtlasiO95881.
    PRIDEiO95881.
    TopDownProteomicsiO95881.

    PTM databases

    iPTMnetiO95881.
    PhosphoSitePlusiO95881.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    BgeeiENSG00000117862.
    CleanExiHS_TXNDC12.
    ExpressionAtlasiO95881. baseline and differential.
    GenevisibleiO95881. HS.

    Interactioni

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi119252. 23 interactors.
    IntActiO95881. 8 interactors.
    MINTiMINT-5005906.
    STRINGi9606.ENSP00000360688.

    Structurei

    Secondary structure

    1172
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi33 – 38Combined sources6
    Helixi43 – 53Combined sources11
    Beta strandi57 – 62Combined sources6
    Helixi67 – 77Combined sources11
    Helixi80 – 86Combined sources7
    Beta strandi89 – 95Combined sources7
    Helixi96 – 98Combined sources3
    Helixi103 – 105Combined sources3
    Beta strandi112 – 118Combined sources7
    Turni120 – 122Combined sources3
    Turni135 – 139Combined sources5
    Helixi144 – 158Combined sources15
    Helixi159 – 161Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1SENX-ray1.20A23-172[»]
    2K8VNMR-A24-172[»]
    ProteinModelPortaliO95881.
    SMRiO95881.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95881.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini27 – 156ThioredoxinPROSITE-ProRule annotationAdd BLAST130

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi169 – 172Prevents secretion from ERSequence analysis4

    Keywords - Domaini

    Redox-active center, Signal

    Phylogenomic databases

    eggNOGiENOG410IWGE. Eukaryota.
    ENOG4111X7E. LUCA.
    GeneTreeiENSGT00530000063273.
    HOGENOMiHOG000231100.
    HOVERGENiHBG107174.
    InParanoidiO95881.
    KOiK05360.
    OMAiGNPNYKY.
    OrthoDBiEOG091G0RHV.
    PhylomeDBiO95881.
    TreeFamiTF321449.

    Family and domain databases

    InterProiView protein in InterPro
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiView protein in PROSITE
    PS00194. THIOREDOXIN_1. 1 hit.
    PS51352. THIOREDOXIN_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O95881-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    METRPRLGAT CLLGFSFLLL VISSDGHNGL GKGFGDHIHW RTLEDGKKEA
    60 70 80 90 100
    AASGLPLMVI IHKSWCGACK ALKPKFAEST EISELSHNFV MVNLEDEEEP
    110 120 130 140 150
    KDEDFSPDGG YIPRILFLDP SGKVHPEIIN ENGNPSYKYF YVSAEQVVQG
    160 170
    MKEAQERLTG DAFRKKHLED EL
    Length:172
    Mass (Da):19,206
    Last modified:May 1, 1999 - v1
    Checksum:i3092E9515A7C4094
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti102D → H in AAH08913 (PubMed:15489334).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF543416 mRNA. Translation: AAN34781.1.
    AF131758 mRNA. Translation: AAD20035.1.
    AY358982 mRNA. Translation: AAQ89341.1.
    AK075409 mRNA. Translation: BAG52132.1.
    AL445685 Genomic DNA. No translation available.
    BC001493 mRNA. Translation: AAH01493.1.
    BC008953 mRNA. Translation: AAH08953.1.
    BC008913 mRNA. Translation: AAH08913.1.
    CCDSiCCDS561.1.
    RefSeqiNP_056997.1. NM_015913.3.
    UniGeneiHs.476033.

    Genome annotation databases

    EnsembliENST00000371626; ENSP00000360688; ENSG00000117862.
    GeneIDi51060.
    KEGGihsa:51060.
    UCSCiuc001cti.5. human.

    Similar proteinsi

    Entry informationi

    Entry nameiTXD12_HUMAN
    AccessioniPrimary (citable) accession number: O95881
    Secondary accession number(s): B3KQS0, Q5T1T4, Q96H50
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
    Last sequence update: May 1, 1999
    Last modified: August 30, 2017
    This is version 154 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references