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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

DDAH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Proton donorBy similarity1
Active sitei276NucleophileSequence analysis1

GO - Molecular functioni

  • amino acid binding Source: Ensembl
  • catalytic activity Source: ProtInc
  • dimethylargininase activity Source: UniProtKB

GO - Biological processi

  • arginine catabolic process Source: ProtInc
  • citrulline metabolic process Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • nitric oxide biosynthetic process Source: ProtInc
  • nitric oxide mediated signal transduction Source: ProtInc
  • positive regulation of nitric oxide biosynthetic process Source: BHF-UCL
  • regulation of nitric-oxide synthase activity Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciZFISH:HS03493-MONOMER.
BRENDAi3.5.3.18. 2681.
ReactomeiR-HSA-203615. eNOS activation.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Protein G6a
S-phase protein
Gene namesi
Name:DDAH2
Synonyms:DDAH, G6A, NG30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:2716. DDAH2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: HPA
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi23564.
OpenTargetsiENSG00000206395.
ENSG00000213722.
ENSG00000225635.
ENSG00000226634.
ENSG00000227317.
ENSG00000228128.
ENSG00000233076.
PharmGKBiPA27186.

Chemistry databases

DrugBankiDB00155. L-Citrulline.

Polymorphism and mutation databases

BioMutaiDDAH2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001711211 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2Add BLAST285

Proteomic databases

EPDiO95865.
PaxDbiO95865.
PeptideAtlasiO95865.
PRIDEiO95865.

2D gel databases

REPRODUCTION-2DPAGEIPI00000760.
O95865.

PTM databases

iPTMnetiO95865.
PhosphoSitePlusiO95865.

Expressioni

Tissue specificityi

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.1 Publication

Gene expression databases

BgeeiENSG00000206395.
CleanExiHS_DDAH2.
ExpressionAtlasiO95865. baseline and differential.
GenevisibleiO95865. HS.

Organism-specific databases

HPAiHPA012509.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIP13Q156455EBI-749139,EBI-358993

Protein-protein interaction databases

BioGridi117107. 35 interactors.
IntActiO95865. 21 interactors.
MINTiMINT-5000845.
STRINGi9606.ENSP00000364943.

Structurei

3D structure databases

ProteinModelPortaliO95865.
SMRiO95865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiO95865.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
PhylomeDBiO95865.
TreeFamiTF314737.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.

Sequencei

Sequence statusi: Complete.

O95865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA
210 220 230 240 250
VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHS
Length:285
Mass (Da):29,644
Last modified:May 1, 1999 - v1
Checksum:iB3D9B00F29492548
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070667 mRNA. Translation: AAD20973.1.
AJ012008 Genomic DNA. Translation: CAB46079.1.
AF087894 mRNA. Translation: AAP97193.1.
AF129756 Genomic DNA. Translation: AAD18074.1.
BA000025 Genomic DNA. Translation: BAB63377.1.
AL662899 Genomic DNA. Translation: CAI18413.1.
AL670886 Genomic DNA. Translation: CAI17820.1.
AL844216 Genomic DNA. Translation: CAM45772.1.
BX248244 Genomic DNA. Translation: CAM26098.1.
CR354443 Genomic DNA. Translation: CAQ07022.1.
CR759787 Genomic DNA. Translation: CAQ10110.1.
CR936239 Genomic DNA. Translation: CAQ08895.1.
CH471081 Genomic DNA. Translation: EAX03500.1.
BC001435 mRNA. Translation: AAH01435.1.
CCDSiCCDS4718.1.
RefSeqiNP_001289936.1. NM_001303007.1.
NP_001289937.1. NM_001303008.1.
NP_039268.1. NM_013974.2.
XP_011512750.1. XM_011514448.2.
UniGeneiHs.247362.

Genome annotation databases

EnsembliENST00000375787; ENSP00000364943; ENSG00000213722.
ENST00000375789; ENSP00000364945; ENSG00000213722.
ENST00000375792; ENSP00000364949; ENSG00000213722.
ENST00000383409; ENSP00000372901; ENSG00000206395.
ENST00000400062; ENSP00000382935; ENSG00000206395.
ENST00000400063; ENSP00000382936; ENSG00000206395.
ENST00000411456; ENSP00000409396; ENSG00000226634.
ENST00000413532; ENSP00000402594; ENSG00000226634.
ENST00000413655; ENSP00000412800; ENSG00000233076.
ENST00000414455; ENSP00000404342; ENSG00000225635.
ENST00000424790; ENSP00000391632; ENSG00000233076.
ENST00000426149; ENSP00000412327; ENSG00000228128.
ENST00000427126; ENSP00000395372; ENSG00000228128.
ENST00000430482; ENSP00000408148; ENSG00000227317.
ENST00000434464; ENSP00000391833; ENSG00000228128.
ENST00000437889; ENSP00000399023; ENSG00000227317.
ENST00000443533; ENSP00000389552; ENSG00000226634.
ENST00000444699; ENSP00000404851; ENSG00000225635.
ENST00000447101; ENSP00000405515; ENSG00000233076.
ENST00000451411; ENSP00000403154; ENSG00000227317.
ENST00000454138; ENSP00000399357; ENSG00000225635.
GeneIDi23564.
KEGGihsa:23564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070667 mRNA. Translation: AAD20973.1.
AJ012008 Genomic DNA. Translation: CAB46079.1.
AF087894 mRNA. Translation: AAP97193.1.
AF129756 Genomic DNA. Translation: AAD18074.1.
BA000025 Genomic DNA. Translation: BAB63377.1.
AL662899 Genomic DNA. Translation: CAI18413.1.
AL670886 Genomic DNA. Translation: CAI17820.1.
AL844216 Genomic DNA. Translation: CAM45772.1.
BX248244 Genomic DNA. Translation: CAM26098.1.
CR354443 Genomic DNA. Translation: CAQ07022.1.
CR759787 Genomic DNA. Translation: CAQ10110.1.
CR936239 Genomic DNA. Translation: CAQ08895.1.
CH471081 Genomic DNA. Translation: EAX03500.1.
BC001435 mRNA. Translation: AAH01435.1.
CCDSiCCDS4718.1.
RefSeqiNP_001289936.1. NM_001303007.1.
NP_001289937.1. NM_001303008.1.
NP_039268.1. NM_013974.2.
XP_011512750.1. XM_011514448.2.
UniGeneiHs.247362.

3D structure databases

ProteinModelPortaliO95865.
SMRiO95865.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117107. 35 interactors.
IntActiO95865. 21 interactors.
MINTiMINT-5000845.
STRINGi9606.ENSP00000364943.

Chemistry databases

DrugBankiDB00155. L-Citrulline.

PTM databases

iPTMnetiO95865.
PhosphoSitePlusiO95865.

Polymorphism and mutation databases

BioMutaiDDAH2.

2D gel databases

REPRODUCTION-2DPAGEIPI00000760.
O95865.

Proteomic databases

EPDiO95865.
PaxDbiO95865.
PeptideAtlasiO95865.
PRIDEiO95865.

Protocols and materials databases

DNASUi23564.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375787; ENSP00000364943; ENSG00000213722.
ENST00000375789; ENSP00000364945; ENSG00000213722.
ENST00000375792; ENSP00000364949; ENSG00000213722.
ENST00000383409; ENSP00000372901; ENSG00000206395.
ENST00000400062; ENSP00000382935; ENSG00000206395.
ENST00000400063; ENSP00000382936; ENSG00000206395.
ENST00000411456; ENSP00000409396; ENSG00000226634.
ENST00000413532; ENSP00000402594; ENSG00000226634.
ENST00000413655; ENSP00000412800; ENSG00000233076.
ENST00000414455; ENSP00000404342; ENSG00000225635.
ENST00000424790; ENSP00000391632; ENSG00000233076.
ENST00000426149; ENSP00000412327; ENSG00000228128.
ENST00000427126; ENSP00000395372; ENSG00000228128.
ENST00000430482; ENSP00000408148; ENSG00000227317.
ENST00000434464; ENSP00000391833; ENSG00000228128.
ENST00000437889; ENSP00000399023; ENSG00000227317.
ENST00000443533; ENSP00000389552; ENSG00000226634.
ENST00000444699; ENSP00000404851; ENSG00000225635.
ENST00000447101; ENSP00000405515; ENSG00000233076.
ENST00000451411; ENSP00000403154; ENSG00000227317.
ENST00000454138; ENSP00000399357; ENSG00000225635.
GeneIDi23564.
KEGGihsa:23564.

Organism-specific databases

CTDi23564.
DisGeNETi23564.
GeneCardsiDDAH2.
HGNCiHGNC:2716. DDAH2.
HPAiHPA012509.
MIMi604744. gene.
neXtProtiNX_O95865.
OpenTargetsiENSG00000206395.
ENSG00000213722.
ENSG00000225635.
ENSG00000226634.
ENSG00000227317.
ENSG00000228128.
ENSG00000233076.
PharmGKBiPA27186.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiO95865.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
PhylomeDBiO95865.
TreeFamiTF314737.

Enzyme and pathway databases

BioCyciZFISH:HS03493-MONOMER.
BRENDAi3.5.3.18. 2681.
ReactomeiR-HSA-203615. eNOS activation.

Miscellaneous databases

ChiTaRSiDDAH2. human.
GenomeRNAii23564.
PROiO95865.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000206395.
CleanExiHS_DDAH2.
ExpressionAtlasiO95865. baseline and differential.
GenevisibleiO95865. HS.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH2_HUMAN
AccessioniPrimary (citable) accession number: O95865
Secondary accession number(s): A2BEZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.