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O95848 (NUD14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uridine diphosphate glucose pyrophosphatase

Short name=UDPG pyrophosphatase
Short name=UGPPase
EC=3.6.1.45
Alternative name(s):
Nucleoside diphosphate-linked moiety X motif 14
Short name=Nudix motif 14
Gene names
Name:NUDT14
Synonyms:UGPP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.

Catalytic activity

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.

Cofactor

Magnesium.

Subunit structure

Homodimer. Ref.2

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the Nudix hydrolase family.

Contains 1 nudix hydrolase domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.0-9.5.

Sequence caution

The sequence AAD15563.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARAFP103983EBI-536866,EBI-365961

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 222222Uridine diphosphate glucose pyrophosphatase
PRO_0000057113

Regions

Domain38 – 206169Nudix hydrolase
Motif111 – 12919Nudix box

Secondary structure

................................ 222
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95848 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: FB1DFA40A67E72B6

FASTA22224,118
        10         20         30         40         50         60 
MERIEGASVG RCAASPYLRP LTLHYRQNGA QKSWDFMKTH DSVTVLLFNS SRRSLVLVKQ 

        70         80         90        100        110        120 
FRPAVYAGEV ERRFPGSLAA VDQDGPRELQ PALPGSAGVT VELCAGLVDQ PGLSLEEVAC 

       130        140        150        160        170        180 
KEAWEECGYH LAPSDLRRVA TYWSGVGLTG SRQTMFYTEV TDAQRSGPGG GLVEEGELIE 

       190        200        210        220 
VVHLPLEGAQ AFADDPDIPK TLGVIFGVSW FLSQVAPNLD LQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization, chromosomal localization, and the complete 30-kb DNA sequence of the human Jagged2 (JAG2) gene."
Deng Y., Madan A., Banta A.B., Friedman C., Trask B.J., Hood L., Li L.
Genomics 63:133-138(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose."
Yagi T., Baroja-Fernandez E., Yamamoto R., Munoz F.J., Akazawa T., Hong K.S., Pozueta-Romero J.
Biochem. J. 370:409-415(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, SUBCELLULAR LOCATION, SUBUNIT.
Tissue: Thyroid.
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[5]"Crystal structure of human uridine diphosphate glucose pyrophosphatase (NUDT14)."
Structural genomics consortium (SGC)
Submitted (FEB-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-222.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF111170 Genomic DNA. Translation: AAD15563.1. Different initiation.
AB087802 mRNA. Translation: BAC65455.1.
AL512355 Genomic DNA. No translation available.
BC041584 mRNA. Translation: AAH41584.1.
CCDSCCDS10000.1.
RefSeqNP_803877.2. NM_177533.4.
UniGeneHs.526432.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3Q91X-ray2.70A/B/C/D28-222[»]
ProteinModelPortalO95848.
SMRO95848. Positions 39-220.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129152. 2 interactions.
IntActO95848. 2 interactions.
STRING9606.ENSP00000339175.

PTM databases

PhosphoSiteO95848.

Proteomic databases

MaxQBO95848.
PaxDbO95848.
PRIDEO95848.

Protocols and materials databases

DNASU256281.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000392568; ENSP00000376349; ENSG00000183828.
GeneID256281.
KEGGhsa:256281.
UCSCuc010tyn.3. human.

Organism-specific databases

CTD256281.
GeneCardsGC14M105639.
HGNCHGNC:20141. NUDT14.
HPAHPA046755.
MIM609219. gene.
neXtProtNX_O95848.
PharmGKBPA134971372.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0494.
HOGENOMHOG000102292.
HOVERGENHBG052689.
InParanoidO95848.
KOK08077.
OMACQICAES.
OrthoDBEOG7JHM6K.
PhylomeDBO95848.
TreeFamTF313661.

Gene expression databases

ArrayExpressO95848.
BgeeO95848.
CleanExHS_NUDT14.
GenevestigatorO95848.

Family and domain databases

Gene3D3.90.79.10. 1 hit.
InterProIPR004385. NDP_pyrophosphatase.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMSSF55811. SSF55811. 1 hit.
TIGRFAMsTIGR00052. TIGR00052. 1 hit.
PROSITEPS51462. NUDIX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95848.
GenomeRNAi256281.
NextBio92764.
PROO95848.
SOURCESearch...

Entry information

Entry nameNUD14_HUMAN
AccessionPrimary (citable) accession number: O95848
Secondary accession number(s): Q86SJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM