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O95848

- NUD14_HUMAN

UniProt

O95848 - NUD14_HUMAN

Protein

Uridine diphosphate glucose pyrophosphatase

Gene

NUDT14

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.

    Catalytic activityi

    UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.

    Cofactori

    Magnesium.

    pH dependencei

    Optimum pH is 8.0-9.5.

    GO - Molecular functioni

    1. ADP-ribose diphosphatase activity Source: Ensembl
    2. metal ion binding Source: InterPro
    3. protein binding Source: IntAct
    4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uridine diphosphate glucose pyrophosphatase (EC:3.6.1.45)
    Short name:
    UDPG pyrophosphatase
    Short name:
    UGPPase
    Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 14
    Short name:
    Nudix motif 14
    Gene namesi
    Name:NUDT14
    Synonyms:UGPP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:20141. NUDT14.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134971372.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Uridine diphosphate glucose pyrophosphatasePRO_0000057113Add
    BLAST

    Proteomic databases

    MaxQBiO95848.
    PaxDbiO95848.
    PRIDEiO95848.

    PTM databases

    PhosphoSiteiO95848.

    Expressioni

    Gene expression databases

    ArrayExpressiO95848.
    BgeeiO95848.
    CleanExiHS_NUDT14.
    GenevestigatoriO95848.

    Organism-specific databases

    HPAiHPA046755.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARAFP103983EBI-536866,EBI-365961

    Protein-protein interaction databases

    BioGridi129152. 2 interactions.
    IntActiO95848. 2 interactions.
    STRINGi9606.ENSP00000339175.

    Structurei

    Secondary structure

    1
    222
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi42 – 498
    Helixi50 – 523
    Beta strandi54 – 618
    Helixi63 – 697
    Beta strandi99 – 1024
    Beta strandi104 – 1074
    Beta strandi110 – 1123
    Helixi115 – 12713
    Helixi133 – 1353
    Beta strandi137 – 1448
    Beta strandi151 – 16010
    Helixi162 – 1643
    Beta strandi179 – 1857
    Helixi186 – 1883
    Helixi189 – 1946
    Helixi202 – 21413
    Helixi216 – 2183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3Q91X-ray2.70A/B/C/D28-222[»]
    ProteinModelPortaliO95848.
    SMRiO95848. Positions 39-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95848.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 206169Nudix hydrolasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi111 – 12919Nudix boxAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family.Curated
    Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0494.
    HOGENOMiHOG000102292.
    HOVERGENiHBG052689.
    InParanoidiO95848.
    KOiK08077.
    OMAiCQICAES.
    OrthoDBiEOG7JHM6K.
    PhylomeDBiO95848.
    TreeFamiTF313661.

    Family and domain databases

    Gene3Di3.90.79.10. 1 hit.
    InterProiIPR004385. NDP_pyrophosphatase.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    [Graphical view]
    PfamiPF00293. NUDIX. 1 hit.
    [Graphical view]
    SUPFAMiSSF55811. SSF55811. 1 hit.
    TIGRFAMsiTIGR00052. TIGR00052. 1 hit.
    PROSITEiPS51462. NUDIX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O95848-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERIEGASVG RCAASPYLRP LTLHYRQNGA QKSWDFMKTH DSVTVLLFNS    50
    SRRSLVLVKQ FRPAVYAGEV ERRFPGSLAA VDQDGPRELQ PALPGSAGVT 100
    VELCAGLVDQ PGLSLEEVAC KEAWEECGYH LAPSDLRRVA TYWSGVGLTG 150
    SRQTMFYTEV TDAQRSGPGG GLVEEGELIE VVHLPLEGAQ AFADDPDIPK 200
    TLGVIFGVSW FLSQVAPNLD LQ 222
    Length:222
    Mass (Da):24,118
    Last modified:March 1, 2004 - v2
    Checksum:iFB1DFA40A67E72B6
    GO

    Sequence cautioni

    The sequence AAD15563.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111170 Genomic DNA. Translation: AAD15563.1. Different initiation.
    AB087802 mRNA. Translation: BAC65455.1.
    AL512355 Genomic DNA. No translation available.
    BC041584 mRNA. Translation: AAH41584.1.
    CCDSiCCDS10000.1.
    RefSeqiNP_803877.2. NM_177533.4.
    UniGeneiHs.526432.

    Genome annotation databases

    EnsembliENST00000392568; ENSP00000376349; ENSG00000183828.
    GeneIDi256281.
    KEGGihsa:256281.
    UCSCiuc010tyn.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF111170 Genomic DNA. Translation: AAD15563.1 . Different initiation.
    AB087802 mRNA. Translation: BAC65455.1 .
    AL512355 Genomic DNA. No translation available.
    BC041584 mRNA. Translation: AAH41584.1 .
    CCDSi CCDS10000.1.
    RefSeqi NP_803877.2. NM_177533.4.
    UniGenei Hs.526432.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3Q91 X-ray 2.70 A/B/C/D 28-222 [» ]
    ProteinModelPortali O95848.
    SMRi O95848. Positions 39-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129152. 2 interactions.
    IntActi O95848. 2 interactions.
    STRINGi 9606.ENSP00000339175.

    PTM databases

    PhosphoSitei O95848.

    Proteomic databases

    MaxQBi O95848.
    PaxDbi O95848.
    PRIDEi O95848.

    Protocols and materials databases

    DNASUi 256281.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000392568 ; ENSP00000376349 ; ENSG00000183828 .
    GeneIDi 256281.
    KEGGi hsa:256281.
    UCSCi uc010tyn.3. human.

    Organism-specific databases

    CTDi 256281.
    GeneCardsi GC14M105639.
    HGNCi HGNC:20141. NUDT14.
    HPAi HPA046755.
    MIMi 609219. gene.
    neXtProti NX_O95848.
    PharmGKBi PA134971372.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0494.
    HOGENOMi HOG000102292.
    HOVERGENi HBG052689.
    InParanoidi O95848.
    KOi K08077.
    OMAi CQICAES.
    OrthoDBi EOG7JHM6K.
    PhylomeDBi O95848.
    TreeFami TF313661.

    Miscellaneous databases

    EvolutionaryTracei O95848.
    GenomeRNAii 256281.
    NextBioi 92764.
    PROi O95848.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95848.
    Bgeei O95848.
    CleanExi HS_NUDT14.
    Genevestigatori O95848.

    Family and domain databases

    Gene3Di 3.90.79.10. 1 hit.
    InterProi IPR004385. NDP_pyrophosphatase.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    [Graphical view ]
    Pfami PF00293. NUDIX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55811. SSF55811. 1 hit.
    TIGRFAMsi TIGR00052. TIGR00052. 1 hit.
    PROSITEi PS51462. NUDIX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization, chromosomal localization, and the complete 30-kb DNA sequence of the human Jagged2 (JAG2) gene."
      Deng Y., Madan A., Banta A.B., Friedman C., Trask B.J., Hood L., Li L.
      Genomics 63:133-138(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose."
      Yagi T., Baroja-Fernandez E., Yamamoto R., Munoz F.J., Akazawa T., Hong K.S., Pozueta-Romero J.
      Biochem. J. 370:409-415(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, SUBCELLULAR LOCATION, SUBUNIT.
      Tissue: Thyroid.
    3. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    5. "Crystal structure of human uridine diphosphate glucose pyrophosphatase (NUDT14)."
      Structural genomics consortium (SGC)
      Submitted (FEB-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 28-222.

    Entry informationi

    Entry nameiNUD14_HUMAN
    AccessioniPrimary (citable) accession number: O95848
    Secondary accession number(s): Q86SJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3