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Protein

Guanylyl cyclase-activating protein 3

Gene

GUCA1C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low and inhibits guanylyl cyclases when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of guanylyl cyclase (GC) is a key event in recovery of the dark state of rod photoreceptors following light exposure.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi65 – 761211 PublicationAdd
BLAST
Calcium bindingi101 – 1121221 PublicationAdd
BLAST
Calcium bindingi143 – 1541231 PublicationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • calcium sensitive guanylate cyclase activator activity Source: ProtInc

GO - Biological processi

  • positive regulation of guanylate cyclase activity Source: GOC
  • regulation of rhodopsin mediated signaling pathway Source: Reactome
  • signal transduction Source: ProtInc
  • visual perception Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase-activating protein 3
Short name:
GCAP 3
Alternative name(s):
Guanylate cyclase activator 1C
Gene namesi
Name:GUCA1C
Synonyms:GCAP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:4680. GUCA1C.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29064.

Polymorphism and mutation databases

BioMutaiGUCA1C.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 209208Guanylyl cyclase-activating protein 3PRO_0000073812Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Modified residuei3 – 31Deamidated asparagineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiO95843.
PRIDEiO95843.

Expressioni

Tissue specificityi

Retina.

Gene expression databases

BgeeiO95843.
CleanExiHS_GUCA1C.
ExpressionAtlasiO95843. baseline and differential.
GenevisibleiO95843. HS.

Organism-specific databases

HPAiHPA041597.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000261047.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi26 – 294Combined sources
Beta strandi33 – 364Combined sources
Helixi37 – 437Combined sources
Helixi51 – 6414Combined sources
Beta strandi69 – 735Combined sources
Helixi74 – 8411Combined sources
Helixi89 – 10012Combined sources
Beta strandi105 – 1084Combined sources
Helixi110 – 12011Combined sources
Helixi131 – 14212Combined sources
Beta strandi147 – 1504Combined sources
Helixi152 – 1609Combined sources
Turni161 – 1655Combined sources
Helixi166 – 1727Combined sources
Helixi177 – 1859Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GGZX-ray3.00A/B1-209[»]
ProteinModelPortaliO95843.
SMRiO95843. Positions 22-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 5036EF-hand 1PROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini52 – 8736EF-hand 2PROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini88 – 12336EF-hand 3PROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini130 – 16536EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Domaini

Binds three calcium ions (via EF-hand 2, 3 and 4).1 Publication

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiO95843.
KOiK08328.
OMAiMEQKLKW.
PhylomeDBiO95843.
TreeFamiTF333971.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR032992. GUCA1C.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF80. PTHR23055:SF80. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95843-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNGKSIAGD QKAVPTQETH VWYRTFMMEY PSGLQTLHEF KTLLGLQGLN
60 70 80 90 100
QKANKHIDQV YNTFDTNKDG FVDFLEFIAA VNLIMQEKME QKLKWYFKLY
110 120 130 140 150
DADGNGSIDK NELLDMFMAV QALNGQQTLS PEEFINLVFH KIDINNDGEL
160 170 180 190 200
TLEEFINGMA KDQDLLEIVY KSFDFSNVLR VICNGKQPDM ETDSSKSPDK

AGLGKVKMK
Length:209
Mass (Da):23,822
Last modified:January 23, 2007 - v3
Checksum:iA407E7D4AEBBAED3
GO
Isoform 2 (identifier: O95843-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-209: ELTLEEFING...KAGLGKVKMK → NGATLYWITF...RLLQCAESNL

Show »
Length:196
Mass (Da):22,484
Checksum:iB2C199105AC8EDB4
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721V → I.1 Publication
Corresponds to variant rs2715687 [ dbSNP | Ensembl ].
VAR_055632
Natural varianti85 – 851M → V.
Corresponds to variant rs6804162 [ dbSNP | Ensembl ].
VAR_055633
Natural varianti119 – 1191A → V.
Corresponds to variant rs11917716 [ dbSNP | Ensembl ].
VAR_055634
Natural varianti159 – 1591M → V.
Corresponds to variant rs16854916 [ dbSNP | Ensembl ].
VAR_055635

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 20961ELTLE…KVKMK → NGATLYWITFPTKGIDFRRI YQWHGKRSGSPGDCSQELRL LQCAESNL in isoform 2. 1 PublicationVSP_000736Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110001
, AF109998, AF109999, AF110000 Genomic DNA. Translation: AAD19943.1.
AF110002 mRNA. Translation: AAD19944.1.
AF110003 mRNA. Translation: AAD19945.1.
CCDSiCCDS2954.1. [O95843-1]
RefSeqiNP_005450.3. NM_005459.3. [O95843-1]
UniGeneiHs.233363.

Genome annotation databases

EnsembliENST00000261047; ENSP00000261047; ENSG00000138472. [O95843-1]
GeneIDi9626.
KEGGihsa:9626.
UCSCiuc003dxj.3. human. [O95843-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF110001
, AF109998, AF109999, AF110000 Genomic DNA. Translation: AAD19943.1.
AF110002 mRNA. Translation: AAD19944.1.
AF110003 mRNA. Translation: AAD19945.1.
CCDSiCCDS2954.1. [O95843-1]
RefSeqiNP_005450.3. NM_005459.3. [O95843-1]
UniGeneiHs.233363.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GGZX-ray3.00A/B1-209[»]
ProteinModelPortaliO95843.
SMRiO95843. Positions 22-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000261047.

Polymorphism and mutation databases

BioMutaiGUCA1C.

Proteomic databases

PaxDbiO95843.
PRIDEiO95843.

Protocols and materials databases

DNASUi9626.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261047; ENSP00000261047; ENSG00000138472. [O95843-1]
GeneIDi9626.
KEGGihsa:9626.
UCSCiuc003dxj.3. human. [O95843-1]

Organism-specific databases

CTDi9626.
GeneCardsiGUCA1C.
H-InvDBHIX0030717.
HGNCiHGNC:4680. GUCA1C.
HPAiHPA041597.
MIMi605128. gene.
neXtProtiNX_O95843.
PharmGKBiPA29064.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiO95843.
KOiK08328.
OMAiMEQKLKW.
PhylomeDBiO95843.
TreeFamiTF333971.

Enzyme and pathway databases

ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

EvolutionaryTraceiO95843.
GenomeRNAii9626.
PROiO95843.
SOURCEiSearch...

Gene expression databases

BgeeiO95843.
CleanExiHS_GUCA1C.
ExpressionAtlasiO95843. baseline and differential.
GenevisibleiO95843. HS.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR032992. GUCA1C.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF80. PTHR23055:SF80. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily."
    Haeseleer F., Sokal I., Li N., Pettenati M.J., Rao N., Bronson J.D., Wechter R., Baehr W., Palczewski K.
    J. Biol. Chem. 274:6526-6535(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION, MYRISTOYLATION AT GLY-2, VARIANT ILE-72.
    Tissue: Retina.
  2. "The crystal structure of GCAP3 suggests molecular mechanism of GCAP-linked cone dystrophies."
    Stephen R., Palczewski K., Sousa M.C.
    J. Mol. Biol. 359:266-275(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH CALCIUM IONS.

Entry informationi

Entry nameiGUC1C_HUMAN
AccessioniPrimary (citable) accession number: O95843
Secondary accession number(s): O95844, Q9UNM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.