Reviewed, UniProtKB/Swiss-Prot O95833 (CLIC3_HUMAN)

Names and origin

Protein names

Recommended name:
    Chloride intracellular channel protein 3

Gene names

Name:

CLIC3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can insert into membranes and form chloride ion channels. May participate in cellular growth control. Ref.4
Subunit structure	Associated with the C-terminal of ERK7.
Subcellular location	Nucleus. Membrane; Single-pass membrane protein. Cytoplasm. Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity.
Tissue specificity	Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas. Ref.4 Ref.5
Domain	Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.
Sequence similarities	Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAD16450.1 differs from that shown. Reason: Frameshift at position 28.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Voltage-gated channel
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	chloride transport Ref.4 Inferred from mutant phenotype. Source: UniProtKB signal transduction Ref.4 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.4 Inferred from direct assay. Source: UniProtKB
Molecular function	chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.4 Inferred from physical interaction. Source: UniProtKB voltage-gated chloride channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Chloride intracellular channel protein 3		PRO_0000144207
Regions
	Transmembrane	24 – 44	21	After insertion into the membrane Potential
	Domain	12 – 90	79	GST N-terminal
	Domain	68 – 235	168	GST C-terminal
	Region	1 – 88	88	Required for insertion into the membrane By similarity
Natural variations
	Natural variant	38	1	P → H: dbSNP rs2292923.		VAR_020424

Sequences

	Sequence		Length	Mass (Da)
	O95833-1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: CA20E66195950886	FASTA	236	26,648
	10 20 30 40 50 60 MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP DVLKDFAPGS 70 80 90 100 110 120 QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE SNTAGNDVFH KFSAFIKNPV 130 140 150 160 170 180 PAQDEALYQQ LLRALARLDS YLRAPLEHEL AGEPQLRESR RRFLDGDRLT LADCSLLPKL 190 200 210 220 230 HIVDTVCAHF RQAPIPAELR GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder.
[4]	"Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel." Qian Z., Okuhara D., Abe M.K., Rosner M.R. J. Biol. Chem. 274:1621-1627(1999) [PubMed: 9880541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[5]	"Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes." Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B., Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G., Gude N.M. Placenta 28:429-436(2007) [PubMed: 17027078] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.

IPI

IPI00000692.

RefSeq

NP_004660.2.

UniGene

Hs.64746

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FY7	X-ray	1.95	A/B	1-230	[»]

ModBase

Search...

Proteomic databases

PeptideAtlas

O95833.

PRIDE

O95833.

Genome annotation databases

Ensembl

ENSG00000169583. Homo sapiens. [Contig view]

GeneID

9022.

KEGG

hsa:9022.

UCSC

uc004ckj.1. human.

Organism-specific databases

GeneCards

GC09M139008.

H-InvDB

HIX0008568.

HGNC

HGNC:2064. CLIC3.

HPA

HPA005963.

MIM

606533. gene.

PharmGKB

PA26590.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O95833.

HOVERGEN

O95833.

OMA

O95833. FHKFSAF.

Gene expression databases

Bgee

O95833.

CleanEx

HS_CLIC3.

GermOnline

ENSG00000169583. Homo sapiens.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR002946. Int_Cl_channel.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

PRINTS

PR01263. INTCLCHANNEL.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

33801.

SOURCE

Search...

Entry information

Entry name

CLIC3_HUMAN

Accession

Primary (citable) accession number: O95833
Secondary accession number(s): Q5SPZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 13, 2004
Last modified:	July 7, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)