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O95833

- CLIC3_HUMAN

UniProt

O95833 - CLIC3_HUMAN

Protein

Chloride intracellular channel protein 3

Gene

CLIC3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Can insert into membranes and form chloride ion channels. May participate in cellular growth control.1 Publication

    GO - Molecular functioni

    1. chloride channel activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. voltage-gated chloride channel activity Source: InterPro

    GO - Biological processi

    1. chloride transmembrane transport Source: GOC
    2. chloride transport Source: UniProtKB
    3. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Chloride channel, Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Protein family/group databases

    TCDBi1.A.12.1.7. the intracellular chloride channel (clic) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloride intracellular channel protein 3
    Gene namesi
    Name:CLIC3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:2064. CLIC3.

    Subcellular locationi

    Nucleus. Membrane; Single-pass membrane protein. Cytoplasm
    Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity.By similarity

    GO - Cellular componenti

    1. chloride channel complex Source: UniProtKB-KW
    2. cytoplasm Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26590.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 236236Chloride intracellular channel protein 3PRO_0000144207Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi22 ↔ 25In soluble form1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiO95833.
    PaxDbiO95833.
    PeptideAtlasiO95833.
    PRIDEiO95833.

    PTM databases

    PhosphoSiteiO95833.

    Expressioni

    Tissue specificityi

    Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas.2 Publications

    Gene expression databases

    ArrayExpressiO95833.
    BgeeiO95833.
    CleanExiHS_CLIC3.
    GenevestigatoriO95833.

    Organism-specific databases

    HPAiHPA005963.

    Interactioni

    Subunit structurei

    Associated with the C-terminal of ERK7.

    Protein-protein interaction databases

    BioGridi114489. 3 interactions.
    STRINGi9606.ENSP00000360678.

    Structurei

    Secondary structure

    1
    236
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 127
    Beta strandi16 – 194
    Helixi23 – 3513
    Beta strandi40 – 445
    Beta strandi64 – 674
    Beta strandi70 – 723
    Helixi75 – 8511
    Turni88 – 903
    Helixi99 – 1035
    Turni104 – 1074
    Helixi108 – 11710
    Helixi121 – 1233
    Helixi124 – 14320
    Helixi147 – 1526
    Beta strandi162 – 1687
    Helixi171 – 19121
    Helixi200 – 21011
    Helixi213 – 2164
    Helixi222 – 2298

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FY7X-ray1.95A/B1-230[»]
    3KJYX-ray1.95A/B1-230[»]
    ProteinModelPortaliO95833.
    SMRiO95833. Positions 3-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95833.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 9079GST N-terminalAdd
    BLAST
    Domaini91 – 235145GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 8888Required for insertion into the membraneBy similarityAdd
    BLAST

    Domaini

    Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.By similarity

    Sequence similaritiesi

    Belongs to the chloride channel CLIC family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG325572.
    HOGENOMiHOG000231548.
    HOVERGENiHBG050994.
    InParanoidiO95833.
    KOiK05023.
    OMAiEKEFKYT.
    OrthoDBiEOG7X3QR3.
    PhylomeDBiO95833.
    TreeFamiTF315438.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR002946. Int_Cl_channel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF13417. GST_N_3. 1 hit.
    [Graphical view]
    PRINTSiPR01263. INTCLCHANNEL.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O95833-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP    50
    DVLKDFAPGS QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE 100
    SNTAGNDVFH KFSAFIKNPV PAQDEALYQQ LLRALARLDS YLRAPLEHEL 150
    AGEPQLRESR RRFLDGDRLT LADCSLLPKL HIVDTVCAHF RQAPIPAELR 200
    GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR 236
    Length:236
    Mass (Da):26,648
    Last modified:April 13, 2004 - v2
    Checksum:iCA20E66195950886
    GO

    Sequence cautioni

    The sequence AAD16450.1 differs from that shown. Reason: Frameshift at position 28.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti38 – 381P → H.
    Corresponds to variant rs2292923 [ dbSNP | Ensembl ].
    VAR_020424

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL807752 Genomic DNA. Translation: CAI12764.1.
    CH471090 Genomic DNA. Translation: EAW88329.1.
    BC007012 mRNA. Translation: AAH07012.2.
    AF102166 mRNA. Translation: AAD16450.1. Frameshift.
    CCDSiCCDS7021.1.
    RefSeqiNP_004660.2. NM_004669.2.
    UniGeneiHs.64746.

    Genome annotation databases

    EnsembliENST00000494426; ENSP00000419378; ENSG00000169583.
    GeneIDi9022.
    KEGGihsa:9022.
    UCSCiuc004ckj.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL807752 Genomic DNA. Translation: CAI12764.1 .
    CH471090 Genomic DNA. Translation: EAW88329.1 .
    BC007012 mRNA. Translation: AAH07012.2 .
    AF102166 mRNA. Translation: AAD16450.1 . Frameshift.
    CCDSi CCDS7021.1.
    RefSeqi NP_004660.2. NM_004669.2.
    UniGenei Hs.64746.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FY7 X-ray 1.95 A/B 1-230 [» ]
    3KJY X-ray 1.95 A/B 1-230 [» ]
    ProteinModelPortali O95833.
    SMRi O95833. Positions 3-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114489. 3 interactions.
    STRINGi 9606.ENSP00000360678.

    Protein family/group databases

    TCDBi 1.A.12.1.7. the intracellular chloride channel (clic) family.

    PTM databases

    PhosphoSitei O95833.

    Proteomic databases

    MaxQBi O95833.
    PaxDbi O95833.
    PeptideAtlasi O95833.
    PRIDEi O95833.

    Protocols and materials databases

    DNASUi 9022.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000494426 ; ENSP00000419378 ; ENSG00000169583 .
    GeneIDi 9022.
    KEGGi hsa:9022.
    UCSCi uc004ckj.1. human.

    Organism-specific databases

    CTDi 9022.
    GeneCardsi GC09M139889.
    HGNCi HGNC:2064. CLIC3.
    HPAi HPA005963.
    MIMi 606533. gene.
    neXtProti NX_O95833.
    PharmGKBi PA26590.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG325572.
    HOGENOMi HOG000231548.
    HOVERGENi HBG050994.
    InParanoidi O95833.
    KOi K05023.
    OMAi EKEFKYT.
    OrthoDBi EOG7X3QR3.
    PhylomeDBi O95833.
    TreeFami TF315438.

    Miscellaneous databases

    EvolutionaryTracei O95833.
    GeneWikii CLIC3.
    GenomeRNAii 9022.
    NextBioi 33801.
    PROi O95833.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95833.
    Bgeei O95833.
    CleanExi HS_CLIC3.
    Genevestigatori O95833.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR002946. Int_Cl_channel.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    PRINTSi PR01263. INTCLCHANNEL.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Urinary bladder.
    4. "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel."
      Qian Z., Okuhara D., Abe M.K., Rosner M.R.
      J. Biol. Chem. 274:1621-1627(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY.
      Tissue: Fetal brain.
    5. "Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes."
      Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B., Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G., Gude N.M.
      Placenta 28:429-436(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, DISULFIDE BOND.

    Entry informationi

    Entry nameiCLIC3_HUMAN
    AccessioniPrimary (citable) accession number: O95833
    Secondary accession number(s): Q5SPZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3