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O95833 (CLIC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloride intracellular channel protein 3
Gene names
Name:CLIC3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can insert into membranes and form chloride ion channels. May participate in cellular growth control. Ref.4

Subunit structure

Associated with the C-terminal of ERK7.

Subcellular location

Nucleus. Membrane; Single-pass membrane protein. Cytoplasm. Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity. Ref.4 Ref.5

Tissue specificity

Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas. Ref.4 Ref.5

Domain

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.

Sequence similarities

Belongs to the chloride channel CLIC family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence caution

The sequence AAD16450.1 differs from that shown. Reason: Frameshift at position 28.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 236236Chloride intracellular channel protein 3
PRO_0000144207

Regions

Transmembrane24 – 4421Helical; Potential
Domain12 – 9079GST N-terminal
Domain91 – 235145GST C-terminal
Region1 – 8888Required for insertion into the membrane By similarity

Amino acid modifications

Disulfide bond22 ↔ 25In soluble form Ref.6

Natural variations

Natural variant381P → H.
Corresponds to variant rs2292923 [ dbSNP | Ensembl ].
VAR_020424

Secondary structure

.................................... 236
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95833 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: CA20E66195950886

FASTA23626,648
        10         20         30         40         50         60 
MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP DVLKDFAPGS 

        70         80         90        100        110        120 
QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE SNTAGNDVFH KFSAFIKNPV 

       130        140        150        160        170        180 
PAQDEALYQQ LLRALARLDS YLRAPLEHEL AGEPQLRESR RRFLDGDRLT LADCSLLPKL 

       190        200        210        220        230 
HIVDTVCAHF RQAPIPAELR GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Urinary bladder.
[4]"Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel."
Qian Z., Okuhara D., Abe M.K., Rosner M.R.
J. Biol. Chem. 274:1621-1627(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[5]"Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes."
Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B., Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G., Gude N.M.
Placenta 28:429-436(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Structure of human CLIC3 at 2 A resolution."
Littler D.R., Brown L.J., Breit S.N., Perrakis A., Curmi P.M.
Proteins 78:1594-1600(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.
CCDSCCDS7021.1.
RefSeqNP_004660.2. NM_004669.2.
UniGeneHs.64746.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3FY7X-ray1.95A/B1-230[»]
3KJYX-ray1.95A/B1-230[»]
ProteinModelPortalO95833.
SMRO95833. Positions 3-230.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114489. 3 interactions.
STRING9606.ENSP00000360678.

Protein family/group databases

TCDB1.A.12.1.7. the intracellular chloride channel (clic) family.

PTM databases

PhosphoSiteO95833.

Proteomic databases

MaxQBO95833.
PaxDbO95833.
PeptideAtlasO95833.
PRIDEO95833.

Protocols and materials databases

DNASU9022.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000494426; ENSP00000419378; ENSG00000169583.
GeneID9022.
KEGGhsa:9022.
UCSCuc004ckj.1. human.

Organism-specific databases

CTD9022.
GeneCardsGC09M139889.
HGNCHGNC:2064. CLIC3.
HPAHPA005963.
MIM606533. gene.
neXtProtNX_O95833.
PharmGKBPA26590.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG325572.
HOGENOMHOG000231548.
HOVERGENHBG050994.
InParanoidO95833.
KOK05023.
OMAEKEFKYT.
OrthoDBEOG7X3QR3.
PhylomeDBO95833.
TreeFamTF315438.

Gene expression databases

ArrayExpressO95833.
BgeeO95833.
CleanExHS_CLIC3.
GenevestigatorO95833.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR002946. Int_Cl_channel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSPR01263. INTCLCHANNEL.
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95833.
GeneWikiCLIC3.
GenomeRNAi9022.
NextBio33801.
PROO95833.
SOURCESearch...

Entry information

Entry nameCLIC3_HUMAN
AccessionPrimary (citable) accession number: O95833
Secondary accession number(s): Q5SPZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM