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Protein

Chloride intracellular channel protein 3

Gene

CLIC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can insert into membranes and form chloride ion channels. May participate in cellular growth control.1 Publication

GO - Molecular functioni

  • chloride channel activity Source: UniProtKB
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  • chloride transport Source: UniProtKB
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169583-MONOMER.

Protein family/group databases

TCDBi1.A.12.1.7. the intracellular chloride channel (clic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 3
Gene namesi
Name:CLIC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:2064. CLIC3.

Subcellular locationi

  • Nucleus
  • Membrane; Single-pass membrane protein
  • Cytoplasm

  • Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9022.
OpenTargetsiENSG00000169583.
PharmGKBiPA26590.

Polymorphism and mutation databases

BioMutaiCLIC3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001442071 – 236Chloride intracellular channel protein 3Add BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 25In soluble form1 Publication
Modified residuei49PhosphoserineCombined sources1
Modified residuei159PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiO95833.
PaxDbiO95833.
PeptideAtlasiO95833.
PRIDEiO95833.

PTM databases

iPTMnetiO95833.
PhosphoSitePlusiO95833.

Expressioni

Tissue specificityi

Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000169583.
CleanExiHS_CLIC3.
GenevisibleiO95833. HS.

Organism-specific databases

HPAiHPA005963.

Interactioni

Subunit structurei

Associated with the C-terminal of ERK7.

Binary interactionsi

WithEntry#Exp.IntActNotes
EFHC2Q5JST63EBI-10192241,EBI-2349927
POT1Q9NUX52EBI-10192241,EBI-752420

Protein-protein interaction databases

BioGridi114489. 9 interactors.
IntActiO95833. 3 interactors.
STRINGi9606.ENSP00000419378.

Structurei

Secondary structure

1236
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Beta strandi16 – 19Combined sources4
Helixi23 – 35Combined sources13
Beta strandi40 – 44Combined sources5
Beta strandi64 – 67Combined sources4
Beta strandi70 – 72Combined sources3
Helixi75 – 85Combined sources11
Turni88 – 90Combined sources3
Helixi99 – 103Combined sources5
Turni104 – 107Combined sources4
Helixi108 – 117Combined sources10
Helixi121 – 123Combined sources3
Helixi124 – 143Combined sources20
Helixi147 – 152Combined sources6
Beta strandi162 – 168Combined sources7
Helixi171 – 191Combined sources21
Helixi200 – 210Combined sources11
Helixi213 – 216Combined sources4
Helixi222 – 229Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FY7X-ray1.95A/B1-230[»]
3KJYX-ray1.95A/B1-230[»]
ProteinModelPortaliO95833.
SMRiO95833.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95833.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 90GST N-terminalAdd BLAST79
Domaini91 – 235GST C-terminalAdd BLAST145

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 88Required for insertion into the membraneBy similarityAdd BLAST88

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INGX. Eukaryota.
ENOG41128FW. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiO95833.
KOiK05023.
OMAiQDDALYQ.
OrthoDBiEOG091G0IHT.
PhylomeDBiO95833.
TreeFamiTF315438.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030261. CLIC-3.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF266. PTHR11260:SF266. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O95833-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP
60 70 80 90 100
DVLKDFAPGS QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE
110 120 130 140 150
SNTAGNDVFH KFSAFIKNPV PAQDEALYQQ LLRALARLDS YLRAPLEHEL
160 170 180 190 200
AGEPQLRESR RRFLDGDRLT LADCSLLPKL HIVDTVCAHF RQAPIPAELR
210 220 230
GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR
Length:236
Mass (Da):26,648
Last modified:April 13, 2004 - v2
Checksum:iCA20E66195950886
GO

Sequence cautioni

The sequence AAD16450 differs from that shown. Reason: Frameshift at position 28.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02042438P → H.Corresponds to variant rs2292923dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.
CCDSiCCDS7021.1.
RefSeqiNP_004660.2. NM_004669.2.
XP_016870771.1. XM_017015282.1.
UniGeneiHs.64746.

Genome annotation databases

EnsembliENST00000494426; ENSP00000419378; ENSG00000169583.
GeneIDi9022.
KEGGihsa:9022.
UCSCiuc004ckj.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.
CCDSiCCDS7021.1.
RefSeqiNP_004660.2. NM_004669.2.
XP_016870771.1. XM_017015282.1.
UniGeneiHs.64746.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FY7X-ray1.95A/B1-230[»]
3KJYX-ray1.95A/B1-230[»]
ProteinModelPortaliO95833.
SMRiO95833.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114489. 9 interactors.
IntActiO95833. 3 interactors.
STRINGi9606.ENSP00000419378.

Protein family/group databases

TCDBi1.A.12.1.7. the intracellular chloride channel (clic) family.

PTM databases

iPTMnetiO95833.
PhosphoSitePlusiO95833.

Polymorphism and mutation databases

BioMutaiCLIC3.

Proteomic databases

MaxQBiO95833.
PaxDbiO95833.
PeptideAtlasiO95833.
PRIDEiO95833.

Protocols and materials databases

DNASUi9022.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000494426; ENSP00000419378; ENSG00000169583.
GeneIDi9022.
KEGGihsa:9022.
UCSCiuc004ckj.2. human.

Organism-specific databases

CTDi9022.
DisGeNETi9022.
GeneCardsiCLIC3.
HGNCiHGNC:2064. CLIC3.
HPAiHPA005963.
MIMi606533. gene.
neXtProtiNX_O95833.
OpenTargetsiENSG00000169583.
PharmGKBiPA26590.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INGX. Eukaryota.
ENOG41128FW. LUCA.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiO95833.
KOiK05023.
OMAiQDDALYQ.
OrthoDBiEOG091G0IHT.
PhylomeDBiO95833.
TreeFamiTF315438.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169583-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO95833.
GeneWikiiCLIC3.
GenomeRNAii9022.
PROiO95833.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169583.
CleanExiHS_CLIC3.
GenevisibleiO95833. HS.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030261. CLIC-3.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF266. PTHR11260:SF266. 2 hits.
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLIC3_HUMAN
AccessioniPrimary (citable) accession number: O95833
Secondary accession number(s): Q5SPZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.