O95833 (CLIC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloride intracellular channel protein 3

Gene names

Name:

CLIC3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can insert into membranes and form chloride ion channels. May participate in cellular growth control. Ref.4
Subunit structure	Associated with the C-terminal of ERK7.
Subcellular location	Nucleus. Membrane; Single-pass membrane protein. Cytoplasm. Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain By similarity. Ref.4 Ref.5
Tissue specificity	Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas. Ref.4 Ref.5
Domain	Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.
Sequence similarities	Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAD16450.1 differs from that shown. Reason: Frameshift at position 28.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Voltage-gated channel
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Traceable author statement Ref.4. Source: ProtInc
Cellular_component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay Ref.4. Source: UniProtKB nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	chloride channel activity Inferred from mutant phenotype Ref.4. Source: UniProtKB voltage-gated chloride channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 236

236

Chloride intracellular channel protein 3

PRO_0000144207

Regions

Transmembrane

24 – 44

21

Helical; Potential

Domain

12 – 90

79

GST N-terminal

Domain

91 – 235

145

GST C-terminal

Region

1 – 88

88

Required for insertion into the membrane By similarity

Amino acid modifications

Disulfide bond

22 ↔ 25

In soluble form Ref.6

Natural variations

Natural variant

38

1

P → H.
Corresponds to variant rs2292923 [ dbSNP | Ensembl ].

VAR_020424

Secondary structure

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236

Helix Strand Turn

Details...

Beta strand

6 – 12

7

Beta strand

16 – 19

4

Helix

23 – 35

13

Beta strand

40 – 44

5

Beta strand

64 – 67

4

Beta strand

70 – 72

3

Helix

75 – 85

11

Turn

88 – 90

3

Helix

99 – 103

5

Turn

104 – 107

4

Helix

108 – 117

10

Helix

121 – 123

3

Helix

124 – 143

20

Helix

147 – 152

6

Beta strand

162 – 168

7

Helix

171 – 191

21

Helix

200 – 210

11

Helix

213 – 216

4

Helix

222 – 229

8

Sequences

	Sequence		Length	Mass (Da)
	O95833 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: CA20E66195950886	FASTA	236	26,648
	10 20 30 40 50 60 MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP DVLKDFAPGS 70 80 90 100 110 120 QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE SNTAGNDVFH KFSAFIKNPV 130 140 150 160 170 180 PAQDEALYQQ LLRALARLDS YLRAPLEHEL AGEPQLRESR RRFLDGDRLT LADCSLLPKL 190 200 210 220 230 HIVDTVCAHF RQAPIPAELR GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder.
[4]	"Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel." Qian Z., Okuhara D., Abe M.K., Rosner M.R. J. Biol. Chem. 274:1621-1627(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[5]	"Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes." Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B., Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G., Gude N.M. Placenta 28:429-436(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Structure of human CLIC3 at 2 A resolution." Littler D.R., Brown L.J., Breit S.N., Perrakis A., Curmi P.M. Proteins 78:1594-1600(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.

IPI

IPI00000692.

RefSeq

NP_004660.2. NM_004669.2.

UniGene

Hs.64746.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FY7	X-ray	1.95	A/B	1-230	[»]
3KJY	X-ray	1.95	A/B	1-230	[»]

ProteinModelPortal

O95833.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000360678.

PTM databases

PhosphoSite

O95833.

Proteomic databases

PaxDb

O95833.

PeptideAtlas

O95833.

PRIDE

O95833.

Protocols and materials databases

DNASU

9022.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000494426; ENSP00000419378; ENSG00000169583.

GeneID

9022.

KEGG

hsa:9022.

UCSC

uc004ckj.1. human.

Organism-specific databases

CTD

9022.

GeneCards

GC09M139889.

HGNC

HGNC:2064. CLIC3.

HPA

HPA005963.

MIM

606533. gene.

neXtProt

NX_O95833.

PharmGKB

PA26590.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG325572.

HOGENOM

HOG000231548.

HOVERGEN

HBG050994.

InParanoid

O95833.

KO

K05023.

OMA

FHKFSAF.

OrthoDB

EOG4MCX15.

PhylomeDB

O95833.

Gene expression databases

ArrayExpress

O95833.

Bgee

O95833.

CleanEx

HS_CLIC3.

Genevestigator

O95833.

GermOnline

ENSG00000169583. Homo sapiens.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR002946. Int_Cl_channel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

PRINTS

PR01263. INTCLCHANNEL.

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O95833.

GenomeRNAi

9022.

NextBio

33801.

SOURCE

Search...

Entry information

Entry name

CLIC3_HUMAN

Accession

Primary (citable) accession number: O95833
Secondary accession number(s): Q5SPZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.