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O95833

- CLIC3_HUMAN

UniProt

O95833 - CLIC3_HUMAN

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Protein

Chloride intracellular channel protein 3

Gene

CLIC3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Can insert into membranes and form chloride ion channels. May participate in cellular growth control.1 Publication

GO - Molecular functioni

  1. chloride channel activity Source: UniProtKB
  2. voltage-gated chloride channel activity Source: InterPro

GO - Biological processi

  1. chloride transmembrane transport Source: GOC
  2. chloride transport Source: UniProtKB
  3. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Protein family/group databases

TCDBi1.A.12.1.7. the intracellular chloride channel (clic) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 3
Gene namesi
Name:CLIC3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:2064. CLIC3.

Subcellular locationi

Nucleus. Membrane; Single-pass membrane protein. Cytoplasm
Note: Predominantly nuclear. Some protein was found in the cytoplasm. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (By similarity).By similarity

GO - Cellular componenti

  1. chloride channel complex Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB
  3. extracellular vesicular exosome Source: UniProt
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26590.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Chloride intracellular channel protein 3PRO_0000144207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 25In soluble form1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO95833.
PaxDbiO95833.
PeptideAtlasiO95833.
PRIDEiO95833.

PTM databases

PhosphoSiteiO95833.

Expressioni

Tissue specificityi

Detected in placenta (at protein level). Widely expressed. High expression is found in placenta followed by lung and heart. Low expression in skeletal muscle, kidney and pancreas.2 Publications

Gene expression databases

BgeeiO95833.
CleanExiHS_CLIC3.
ExpressionAtlasiO95833. baseline and differential.
GenevestigatoriO95833.

Organism-specific databases

HPAiHPA005963.

Interactioni

Subunit structurei

Associated with the C-terminal of ERK7.

Protein-protein interaction databases

BioGridi114489. 3 interactions.
STRINGi9606.ENSP00000360678.

Structurei

Secondary structure

1
236
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127
Beta strandi16 – 194
Helixi23 – 3513
Beta strandi40 – 445
Beta strandi64 – 674
Beta strandi70 – 723
Helixi75 – 8511
Turni88 – 903
Helixi99 – 1035
Turni104 – 1074
Helixi108 – 11710
Helixi121 – 1233
Helixi124 – 14320
Helixi147 – 1526
Beta strandi162 – 1687
Helixi171 – 19121
Helixi200 – 21011
Helixi213 – 2164
Helixi222 – 2298

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FY7X-ray1.95A/B1-230[»]
3KJYX-ray1.95A/B1-230[»]
ProteinModelPortaliO95833.
SMRiO95833. Positions 3-230.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95833.

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 9079GST N-terminalAdd
BLAST
Domaini91 – 235145GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8888Required for insertion into the membraneBy similarityAdd
BLAST

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG325572.
GeneTreeiENSGT00550000074477.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiO95833.
KOiK05023.
OMAiEKEFKYT.
OrthoDBiEOG7X3QR3.
PhylomeDBiO95833.
TreeFamiTF315438.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR002946. Int_Cl_channel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O95833-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP
60 70 80 90 100
DVLKDFAPGS QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE
110 120 130 140 150
SNTAGNDVFH KFSAFIKNPV PAQDEALYQQ LLRALARLDS YLRAPLEHEL
160 170 180 190 200
AGEPQLRESR RRFLDGDRLT LADCSLLPKL HIVDTVCAHF RQAPIPAELR
210 220 230
GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR
Length:236
Mass (Da):26,648
Last modified:April 13, 2004 - v2
Checksum:iCA20E66195950886
GO

Sequence cautioni

The sequence AAD16450.1 differs from that shown. Reason: Frameshift at position 28.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti38 – 381P → H.
Corresponds to variant rs2292923 [ dbSNP | Ensembl ].
VAR_020424

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL807752 Genomic DNA. Translation: CAI12764.1.
CH471090 Genomic DNA. Translation: EAW88329.1.
BC007012 mRNA. Translation: AAH07012.2.
AF102166 mRNA. Translation: AAD16450.1. Frameshift.
CCDSiCCDS7021.1.
RefSeqiNP_004660.2. NM_004669.2.
UniGeneiHs.64746.

Genome annotation databases

EnsembliENST00000494426; ENSP00000419378; ENSG00000169583.
GeneIDi9022.
KEGGihsa:9022.
UCSCiuc004ckj.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL807752 Genomic DNA. Translation: CAI12764.1 .
CH471090 Genomic DNA. Translation: EAW88329.1 .
BC007012 mRNA. Translation: AAH07012.2 .
AF102166 mRNA. Translation: AAD16450.1 . Frameshift.
CCDSi CCDS7021.1.
RefSeqi NP_004660.2. NM_004669.2.
UniGenei Hs.64746.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3FY7 X-ray 1.95 A/B 1-230 [» ]
3KJY X-ray 1.95 A/B 1-230 [» ]
ProteinModelPortali O95833.
SMRi O95833. Positions 3-230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114489. 3 interactions.
STRINGi 9606.ENSP00000360678.

Protein family/group databases

TCDBi 1.A.12.1.7. the intracellular chloride channel (clic) family.

PTM databases

PhosphoSitei O95833.

Proteomic databases

MaxQBi O95833.
PaxDbi O95833.
PeptideAtlasi O95833.
PRIDEi O95833.

Protocols and materials databases

DNASUi 9022.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000494426 ; ENSP00000419378 ; ENSG00000169583 .
GeneIDi 9022.
KEGGi hsa:9022.
UCSCi uc004ckj.1. human.

Organism-specific databases

CTDi 9022.
GeneCardsi GC09M139889.
HGNCi HGNC:2064. CLIC3.
HPAi HPA005963.
MIMi 606533. gene.
neXtProti NX_O95833.
PharmGKBi PA26590.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG325572.
GeneTreei ENSGT00550000074477.
HOGENOMi HOG000231548.
HOVERGENi HBG050994.
InParanoidi O95833.
KOi K05023.
OMAi EKEFKYT.
OrthoDBi EOG7X3QR3.
PhylomeDBi O95833.
TreeFami TF315438.

Miscellaneous databases

EvolutionaryTracei O95833.
GeneWikii CLIC3.
GenomeRNAii 9022.
NextBioi 33801.
PROi O95833.
SOURCEi Search...

Gene expression databases

Bgeei O95833.
CleanExi HS_CLIC3.
ExpressionAtlasi O95833. baseline and differential.
Genevestigatori O95833.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR002946. Int_Cl_channel.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF13417. GST_N_3. 1 hit.
[Graphical view ]
PRINTSi PR01263. INTCLCHANNEL.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Urinary bladder.
  4. "Molecular cloning and characterization of a mitogen-activated protein kinase-associated intracellular chloride channel."
    Qian Z., Okuhara D., Abe M.K., Rosner M.R.
    J. Biol. Chem. 274:1621-1627(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  5. "Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes."
    Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B., Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G., Gude N.M.
    Placenta 28:429-436(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, DISULFIDE BOND.

Entry informationi

Entry nameiCLIC3_HUMAN
AccessioniPrimary (citable) accession number: O95833
Secondary accession number(s): Q5SPZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 13, 2004
Last modified: October 29, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3