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O95819

- M4K4_HUMAN

UniProt

O95819 - M4K4_HUMAN

Protein

Mitogen-activated protein kinase kinase kinase kinase 4

Gene

MAP4K4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (28 Mar 2003)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB
    4. small GTPase regulator activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB
    2. protein phosphorylation Source: UniProtKB
    3. regulation of JNK cascade Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_169436. Oxidative Stress Induced Senescence.
    SignaLinkiO95819.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase kinase 4 (EC:2.7.11.1)
    Alternative name(s):
    HPK/GCK-like kinase HGK
    MAPK/ERK kinase kinase kinase 4
    Short name:
    MEK kinase kinase 4
    Short name:
    MEKKK 4
    Nck-interacting kinase
    Gene namesi
    Name:MAP4K4
    Synonyms:HGK, KIAA0687, NIK
    OrganismiHomo sapiens (Human)Imported
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:6866. MAP4K4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30612.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 12391238Mitogen-activated protein kinase kinase kinase kinase 4PRO_0000086280Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine2 Publications
    Modified residuei5 – 51Phosphoserine1 Publication
    Modified residuei629 – 6291Phosphoserine2 Publications
    Modified residuei631 – 6311Phosphoserine2 Publications
    Modified residuei639 – 6391Phosphoserine5 Publications
    Modified residuei644 – 6441Phosphoserine2 Publications
    Modified residuei712 – 7121Phosphoserine1 Publication
    Modified residuei800 – 8001Phosphoserine1 Publication
    Modified residuei805 – 8051Phosphoserine5 Publications
    Modified residuei900 – 9001Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO95819.
    PaxDbiO95819.
    PRIDEiO95819.

    PTM databases

    PhosphoSiteiO95819.

    Expressioni

    Tissue specificityi

    Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.1 Publication

    Gene expression databases

    ArrayExpressiO95819.
    BgeeiO95819.
    CleanExiHS_MAP4K4.
    GenevestigatoriO95819.

    Organism-specific databases

    HPAiHPA008476.

    Interactioni

    Subunit structurei

    Interacts with the SH3 domain of the adapter proteins Nck By similarity. Interacts (via its CNH regulatory domain) with ATL1 (via the N-terminal region). Interacts with RAP2A (GTP-bound form preferentially).By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GBP3Q9H0R54EBI-2511133,EBI-2798916

    Protein-protein interaction databases

    BioGridi114838. 22 interactions.
    IntActiO95819. 17 interactions.
    MINTiMINT-1172114.
    STRINGi9606.ENSP00000313644.

    Structurei

    Secondary structure

    1
    1239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi15 – 173
    Turni22 – 243
    Beta strandi25 – 317
    Turni32 – 354
    Beta strandi36 – 449
    Turni45 – 473
    Beta strandi50 – 589
    Helixi66 – 7712
    Beta strandi85 – 917
    Beta strandi99 – 1068
    Helixi113 – 1186
    Helixi121 – 1233
    Helixi127 – 14620
    Helixi156 – 1583
    Beta strandi159 – 1613
    Beta strandi167 – 1693
    Helixi197 – 1993
    Beta strandi205 – 2073
    Helixi212 – 22817
    Turni232 – 2354
    Helixi238 – 24710
    Helixi260 – 26910
    Helixi274 – 2763
    Helixi280 – 2845
    Helixi287 – 2904
    Helixi295 – 30814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4OBOX-ray2.10A/B2-328[»]
    4OBPX-ray2.27A/B2-328[»]
    4OBQX-ray2.19A/B2-328[»]
    ProteinModelPortaliO95819.
    SMRiO95819. Positions 13-408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 289265Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini926 – 1213288CNHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni858 – 1212355Mediates interaction with RAP2AAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CNH domain.CuratedPROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000290708.
    HOVERGENiHBG036506.
    KOiK04407.
    PhylomeDBiO95819.
    TreeFamiTF105138.

    Family and domain databases

    InterProiIPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: O95819-1) [UniParc]FASTAAdd to Basket

    Also known as: Tumor-associated

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL     50
    AAIKVMDVTE DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ 100
    LWLVMEFCGA GSITDLVKNT KGNTLKEDWI AYISREILRG LAHLHIHHVI 150
    HRDIKGQNVL LTENAEVKLV DFGVSAQLDR TVGRRNTFIG TPYWMAPEVI 200
    ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR ALFLIPRNPP 250
    PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI 300
    QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES 350
    TLRRDFLRLQ QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE 400
    QQKEQRRRLE EQQRREREAR RQQEREQRRR EQEEKRRLEE LERRRKEEEE 450
    RRRAEEEKRR VEREQEYIRR QLEEEQRHLE VLQQQLLQEQ AMLLECRWRE 500
    MEEHRQAERL QRQLQQEQAY LLSLQHDHRR PHPQHSQQPP PPQQERSKPS 550
    FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS 600
    ESFSNGNSES VHPALQRPAE PQVPVRTTSR SPVLSRRDSP LQGSGQQNSQ 650
    AGQRNSTSIE PRLLWERVEK LVPRPGSGSS SGSSNSGSQP GSHPGSQSGS 700
    GERFRVRSSS KSEGSPSQRL ENAVKKPEDK KEVFRPLKPA DLTALAKELR 750
    AVEDVRPPHK VTDYSSSSEE SGTTDEEDDD VEQEGADEST SGPEDTRAAS 800
    SLNLSNGETE SVKTMIVHDD VESEPAMTPS KEGTLIVRQT QSASSTLQKH 850
    KSSSSFTPFI DPRLLQISPS SGTTVTSVVG FSCDGMRPEA IRQDPTRKGS 900
    VVNVNPTNTR PQSDTPEIRK YKKRFNSEIL CAALWGVNLL VGTESGLMLL 950
    DRSGQGKVYP LINRRRFQQM DVLEGLNVLV TISGKKDKLR VYYLSWLRNK 1000
    ILHNDPEVEK KQGWTTVGDL EGCVHYKVVK YERIKFLVIA LKSSVEVYAW 1050
    APKPYHKFMA FKSFGELVHK PLLVDLTVEE GQRLKVIYGS CAGFHAVDVD 1100
    SGSVYDIYLP THIQCSIKPH AIIILPNTDG MELLVCYEDE GVYVNTYGRI 1150
    TKDVVLQWGE MPTSVAYIRS NQTMGWGEKA IEIRSVETGH LDGVFMHKRA 1200
    QRLKFLCERN DKVFFASVRS GGSSQVYFMT LGRTSLLSW 1239
    Length:1,239
    Mass (Da):142,101
    Last modified:March 28, 2003 - v2
    Checksum:i8FBBE2F9ABEEC757
    GO
    Isoform 21 Publication (identifier: O95819-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         495-525: Missing.
         740-740: A → AGEV

    Show »
    Length:1,211
    Mass (Da):138,418
    Checksum:iFD4A41765D9719F7
    GO
    Isoform 31 Publication (identifier: O95819-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         623-623: V → VQWSHLASLK...SDSDEVPPRV
         740-740: A → AGEV

    Show »
    Length:1,319
    Mass (Da):150,913
    Checksum:i6DCBFA9AB27A8AD9
    GO
    Isoform 41 Publication (identifier: O95819-4) [UniParc]FASTAAdd to Basket

    Also known as: HGK-S1 Publication

    The sequence of this isoform differs from the canonical sequence as follows:
         495-525: Missing.
         569-622: Missing.
         740-740: A → AGEV
         1112-1112: H → HVRKNPHSM

    Show »
    Length:1,165
    Mass (Da):133,401
    Checksum:i03D9E1A3D7F9415D
    GO
    Isoform 51 Publication (identifier: O95819-5) [UniParc]FASTAAdd to Basket

    Also known as: HGK-L1 Publication

    The sequence of this isoform differs from the canonical sequence as follows:
         495-525: Missing.
         569-622: Missing.
         623-623: V → VQWSHLASLK...SDSDEVPPRV
         740-740: A → AGEV
         1112-1112: H → HVRKNPHSM

    Show »
    Length:1,242
    Mass (Da):141,929
    Checksum:iAF0219B2CA816B5B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti658 – 6581S → SS(PubMed:9734811)Curated
    Sequence conflicti658 – 6581S → SS(PubMed:17974005)Curated
    Sequence conflicti839 – 8391Q → R in AAO32626. (PubMed:12612079)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti712 – 7121S → T.1 Publication
    VAR_040746
    Isoform 31 Publication (identifier: O95819-3)
    Natural varianti682 – 6821D → V.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei495 – 52531Missing in isoform 2, isoform 4 and isoform 5. 2 PublicationsVSP_007054Add
    BLAST
    Alternative sequencei569 – 62254Missing in isoform 4 and isoform 5. 1 PublicationVSP_007055Add
    BLAST
    Alternative sequencei623 – 6231V → VQWSHLASLKNNVSPVSRSH SFSDPSPKFAHHHLRSQDPC PPSRSEVLSQSSDSKSEAPD PTQKAWSRSDSDEVPPRV in isoform 3 and isoform 5. 2 PublicationsVSP_007056
    Alternative sequencei740 – 7401A → AGEV in isoform 2, isoform 3, isoform 4 and isoform 5. 3 PublicationsVSP_007057
    Alternative sequencei1112 – 11121H → HVRKNPHSM in isoform 4 and isoform 5. 1 PublicationVSP_007058

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF096300 mRNA. Translation: AAD16137.1.
    AY212247 mRNA. Translation: AAO32626.1.
    AB014587 mRNA. Translation: BAA31662.1.
    AL137755 mRNA. Translation: CAB70907.2.
    AB013385 mRNA. Translation: BAA33714.1.
    CCDSiCCDS56130.1. [O95819-1]
    PIRiT46481.
    RefSeqiNP_001229488.1. NM_001242559.1. [O95819-1]
    NP_004825.3. NM_004834.4. [O95819-4]
    NP_663720.1. NM_145687.3.
    UniGeneiHs.701013.

    Genome annotation databases

    EnsembliENST00000347699; ENSP00000314363; ENSG00000071054. [O95819-1]
    GeneIDi9448.
    KEGGihsa:9448.
    UCSCiuc002tbc.3. human. [O95819-3]
    uc002tbd.3. human. [O95819-2]
    uc002tbg.3. human. [O95819-1]
    uc002tbh.3. human. [O95819-4]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF096300 mRNA. Translation: AAD16137.1 .
    AY212247 mRNA. Translation: AAO32626.1 .
    AB014587 mRNA. Translation: BAA31662.1 .
    AL137755 mRNA. Translation: CAB70907.2 .
    AB013385 mRNA. Translation: BAA33714.1 .
    CCDSi CCDS56130.1. [O95819-1 ]
    PIRi T46481.
    RefSeqi NP_001229488.1. NM_001242559.1. [O95819-1 ]
    NP_004825.3. NM_004834.4. [O95819-4 ]
    NP_663720.1. NM_145687.3.
    UniGenei Hs.701013.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4OBO X-ray 2.10 A/B 2-328 [» ]
    4OBP X-ray 2.27 A/B 2-328 [» ]
    4OBQ X-ray 2.19 A/B 2-328 [» ]
    ProteinModelPortali O95819.
    SMRi O95819. Positions 13-408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114838. 22 interactions.
    IntActi O95819. 17 interactions.
    MINTi MINT-1172114.
    STRINGi 9606.ENSP00000313644.

    Chemistry

    BindingDBi O95819.
    ChEMBLi CHEMBL6166.
    GuidetoPHARMACOLOGYi 2088.

    PTM databases

    PhosphoSitei O95819.

    Proteomic databases

    MaxQBi O95819.
    PaxDbi O95819.
    PRIDEi O95819.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000347699 ; ENSP00000314363 ; ENSG00000071054 . [O95819-1 ]
    GeneIDi 9448.
    KEGGi hsa:9448.
    UCSCi uc002tbc.3. human. [O95819-3 ]
    uc002tbd.3. human. [O95819-2 ]
    uc002tbg.3. human. [O95819-1 ]
    uc002tbh.3. human. [O95819-4 ]

    Organism-specific databases

    CTDi 9448.
    GeneCardsi GC02P102313.
    HGNCi HGNC:6866. MAP4K4.
    HPAi HPA008476.
    MIMi 604666. gene.
    neXtProti NX_O95819.
    PharmGKBi PA30612.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000290708.
    HOVERGENi HBG036506.
    KOi K04407.
    PhylomeDBi O95819.
    TreeFami TF105138.

    Enzyme and pathway databases

    Reactomei REACT_169436. Oxidative Stress Induced Senescence.
    SignaLinki O95819.

    Miscellaneous databases

    ChiTaRSi MAP4K4. human.
    GeneWikii MAP4K4.
    GenomeRNAii 9448.
    NextBioi 35392.
    PROi O95819.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95819.
    Bgeei O95819.
    CleanExi HS_MAP4K4.
    Genevestigatori O95819.

    Family and domain databases

    InterProi IPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel human STE20-related protein kinase, HGK, that specifically activates the c-Jun N-terminal kinase signaling pathway."
      Yao Z., Zhou G., Wang X.S., Brown A., Diener K., Gan H., Tan T.-H.
      J. Biol. Chem. 274:2118-2125(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 4 AND 5), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Macrophage.
    2. "The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion, and adhesion."
      Wright J.H., Wang X., Manning G., LaMere B.J., Le P., Zhu S., Khatry D., Flanagan P.M., Buckley S.D., Whyte D.B., Howlett A.R., Bischoff J.R., Lipson K.E., Jallal B.
      Mol. Cell. Biol. 23:2068-2082(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Glioblastoma.
    3. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
      Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
      DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-1239 (ISOFORM 2).
      Tissue: Brain.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-1239 (ISOFORM 3).
      Tissue: Testis.
    5. "Isolation, expression profile and chromosome assignment of a novel serine/threonine kinase gene."
      Saito T., Seki N., Hori T.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 700-1239.
      Tissue: Brain.
    6. "A novel GTP-binding protein hGBP3 interacts with NIK/HGK."
      Luan Z., Zhang Y., Liu A., Man Y., Cheng L., Hu G.
      FEBS Lett. 530:233-238(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATL1.
    7. "Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase."
      Machida N., Umikawa M., Takei K., Sakima N., Myagmar B.E., Taira K., Uezato H., Ogawa Y., Kariya K.
      J. Biol. Chem. 279:15711-15714(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAP2A, SUBCELLULAR LOCATION.
    8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639; SER-644; SER-712 AND SER-805, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629; SER-631; SER-639 AND SER-805, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-639; SER-644; SER-805 AND SER-900, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    12. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-800 AND SER-805, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-805, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. Cited for: FUNCTION.
    16. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-629; SER-631 AND SER-639, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-712, VARIANT [LARGE SCALE ANALYSIS] VAL-682 (ISOFORM 3).

    Entry informationi

    Entry nameiM4K4_HUMAN
    AccessioniPrimary (citable) accession number: O95819
    Secondary accession number(s): O75172, Q9NST7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: March 28, 2003
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3