O95813 (CER1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cerberus

Alternative name(s):
Cerberus-related protein
DAN domain family member 4

Gene names

Name:	CER1
Synonyms:	DAND4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine that may play a role in anterior neural induction and somite formation during embryogenesis in part through a BMP-inhibitory mechanism. Can regulate Nodal signaling during gastrulation as well as the formation and patterning of the primitive streak By similarity.
Subunit structure	Forms monomers and predominantly dimers By similarity.
Subcellular location	Secreted Probable.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the DAN family. Contains 1 CTCK (C-terminal cystine knot-like) domain.
Sequence caution	The sequence AAH69503.1 differs from that shown. Reason: Erroneous termination at position 266. Translated as Ser.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	BMP signaling pathway Traceable author statement. Source: Reactome anterior/posterior axis specification Inferred from sequence or structural similarity Ref.1. Source: BHF-UCL bone mineralization Inferred from mutant phenotype PubMed 19113921. Source: BHF-UCL cell migration involved in gastrulation Inferred from sequence or structural similarity Ref.1. Source: BHF-UCL cellular response to BMP stimulus Inferred from sequence or structural similarity PubMed 16798745. Source: BHF-UCL determination of dorsal identity Inferred from mutant phenotype PubMed 9660951. Source: BHF-UCL growth plate cartilage chondrocyte proliferation Inferred from sequence or structural similarity PubMed 19113921. Source: BHF-UCL negative regulation of activin receptor signaling pathway Inferred from direct assay PubMed 9660951. Source: BHF-UCL negative regulation of cell proliferation Inferred from sequence or structural similarity Ref.1. Source: BHF-UCL negative regulation of mesoderm development Inferred from mutant phenotype PubMed 9660951. Source: BHF-UCL nervous system development Inferred from mutant phenotype PubMed 9660951. Source: BHF-UCL sequestering of BMP in extracellular matrix Inferred from direct assay PubMed 9660951. Source: BHF-UCL signal transduction involved in regulation of gene expression Inferred from sequence or structural similarity Ref.1. Source: BHF-UCL ureteric bud development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular space Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	BMP binding Inferred from direct assay PubMed 9660951. Source: BHF-UCL morphogen activity Inferred from direct assay PubMed 9660951. Source: BHF-UCL protein homodimerization activity Inferred from sequence or structural similarity. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.5
	Chain	18 – 267	250	Cerberus		PRO_0000006711
Regions
	Domain	162 – 246	85	CTCK
Amino acid modifications
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	162 ↔ 209		By similarity
	Disulfide bond	176 ↔ 223		By similarity
	Disulfide bond	186 ↔ 239		By similarity
	Disulfide bond	190 ↔ 241		By similarity
Natural variations
	Natural variant	19	1	R → W. Ref.4 Corresponds to variant rs10115703 [ dbSNP | Ensembl ].		VAR_021591
	Natural variant	65	1	A → G. Ref.4 Corresponds to variant rs3747532 [ dbSNP | Ensembl ].		VAR_021592
	Natural variant	179	1	V → I. Ref.4 Corresponds to variant rs7036635 [ dbSNP | Ensembl ].		VAR_021593
Experimental info
	Sequence conflict	57	1	F → L in AAH69503. Ref.4
	Sequence conflict	221	1	L → V in AAH69405. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O95813 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: C9FB048CD8558ED7	FASTA	267	30,084
	10 20 30 40 50 60 MHLLLFQLLV LLPLGKTTRH QDGRQNQSSL SPVLLPRNQR ELPTGNHEEA EEKPDLFVAV 70 80 90 100 110 120 PHLVATSPAG EGQRQREKML SRFGRFWKKP EREMHPSRDS DSEPFPPGTQ SLIQPIDGMK 130 140 150 160 170 180 MEKSPLREEA KKFWHHFMFR KTPASQGVIL PIKSHEVHWE TCRTVPFSQT ITHEGCEKVV 190 200 210 220 230 240 VQNNLCFGKC GSVHFPGAAQ HSHTSCSHCL PAKFTTMHLP LNCTELSSVI KVVMLVEECQ 250 260 CKVKTEHEDG HILHAGSQDS FIPGVSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human cerberus related gene CER1 maps to chromosome 9." Lah M., Brodnicki T., Maccarone P., Nash A., Stanley E., Harvey R.P. Genomics 55:364-366(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
[2]	Feng Z., Zhang B., Peng X., Yuan J., Qiang B. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS TRP-19; GLY-65 AND ILE-179.
[5]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-32.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF090189 Genomic DNA. Translation: AAD19879.1. AF400435 mRNA. Translation: AAK92484.1. AL390732 Genomic DNA. Translation: CAH73850.1. BC069371 mRNA. Translation: AAH69371.1. BC069405 mRNA. Translation: AAH69405.1. BC069491 mRNA. Translation: AAH69491.1. BC069503 mRNA. Translation: AAH69503.1. Sequence problems.
IPI	IPI00023129.
RefSeq	NP_005445.1. NM_005454.2.
UniGene	Hs.248204.
3D structure databases
ProteinModelPortal	O95813.
SMR	O95813. Positions 145-246.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000370297.
PTM databases
PhosphoSite	O95813.
Proteomic databases
PaxDb	O95813.
PRIDE	O95813.
Protocols and materials databases
DNASU	9350.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380911; ENSP00000370297; ENSG00000147869.
GeneID	9350.
KEGG	hsa:9350.
UCSC	uc003zlj.3. human.
Organism-specific databases
CTD	9350.
GeneCards	GC09M014710.
H-InvDB	HIX0034784.
HGNC	HGNC:1862. CER1.
HPA	HPA019917.
MIM	603777. gene.
neXtProt	NX_O95813.
PharmGKB	PA26417.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG40714.
HOGENOM	HOG000231309.
HOVERGEN	HBG050902.
InParanoid	O95813.
KO	K01645.
OMA	CFGKCGS.
OrthoDB	EOG437RFQ.
PhylomeDB	O95813.
Enzyme and pathway databases
Pathway_Interaction_DB	bmppathway. BMP receptor signaling.
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction.
Gene expression databases
Bgee	O95813.
CleanEx	HS_CER1.
Genevestigator	O95813.
GermOnline	ENSG00000147869. Homo sapiens.
Family and domain databases
InterPro	IPR016860. Cerberus. IPR006207. Cys_knot_C. IPR004133. DAN. [Graphical view]
Pfam	PF03045. DAN. 1 hit. [Graphical view]
PIRSF	PIRSF027807. Cerberus. 1 hit.
SMART	SM00041. CT. 1 hit. [Graphical view]
PROSITE	PS01185. CTCK_1. False negative. PS01225. CTCK_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9350.
NextBio	35013.
SOURCE	Search...

Entry information

Entry name

CER1_HUMAN

Accession

Primary (citable) accession number: O95813
Secondary accession number(s): Q6ISJ1 , Q6ISJ6, Q6ISQ2, Q6ISS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.