O95793 (STAU1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Double-stranded RNA-binding protein Staufen homolog 1

Gene names

Name:	STAU1
Synonyms:	STAU

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
Subunit structure	Binds tubulin. Binds with low affinity single-stranded RNA or DNA homopolymers. Interacts with CASC3 in an RNA-dependent manner By similarity. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with the influenza virus nonstructural protein NS1. Ref.9
Subcellular location	Cytoplasm. Rough endoplasmic reticulum. Note: Localizes exclusively with the rough reticulum endoplasmic (RER). Ref.9
Tissue specificity	Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
Domain	One of the DRDB could be involved in RER binding. The C-terminal contains the tubulin binding domain (TBD) By similarity.
Sequence similarities	Contains 3 DRBM (double-stranded RNA-binding) domains.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular mRNA localization Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasmic stress granule Inferred from sequence or structural similarity. Source: BHF-UCL microtubule associated complex Traceable author statement Ref.1. Source: ProtInc rough endoplasmic reticulum Traceable author statement Ref.1. Source: ProtInc
Molecular_function	double-stranded RNA binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPL7A	P62424	2	EBI-358174,EBI-354172
RPLP0	P05388	4	EBI-358174,EBI-354101
UPF1	Q92900	4	EBI-358174,EBI-373471

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: O95793-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O95793-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Isoform 3 (identifier: O95793-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.
     203-203: E → ESFPLKQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Double-stranded RNA-binding protein Staufen homolog 1		PRO_0000072243
Regions
	Domain	72 – 162	91	DRBM 1
	Domain	184 – 251	68	DRBM 2
	Domain	286 – 354	69	DRBM 3
Amino acid modifications
	Modified residue	390	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	1 – 81	81	Missing in isoform Short and isoform 3.		VSP_004434
	Alternative sequence	203	1	E → ESFPLKQ in isoform 3.		VSP_043701
Experimental info
	Sequence conflict	359	1	A → R Ref.1
	Sequence conflict	359	1	A → R Ref.2
	Sequence conflict	360 – 392	33	QPTKP…PGSGD → SHQTRTQVRGEDTHKETRGW KKSNLFLNLALGM Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 0977CEF40FD99517	FASTA	577	63,182
	10 20 30 40 50 60 MSQVQVQVQN PSAALSGSQI LNKNQSLLSQ PLMSIPSTTS SLPSENAGRP IQNSALPSAS 70 80 90 100 110 120 ITSTSAAAES ITPTVELNAL CMKLGKKPMY KPVDPYSRMQ STYNYNMRGG AYPPRYFYPF 130 140 150 160 170 180 PVPPLLYQVE LSVGGQQFNG KGKTRQAAKH DAAAKALRIL QNEPLPERLE VNGRESEEEN 190 200 210 220 230 240 LNKSEISQVF EIALKRNLPV NFEVARESGP PHMKNFVTKV SVGEFVGEGE GKSKKISKKN 250 260 270 280 290 300 AAIAVLEELK KLPPLPAVER VKPRIKKKTK PIVKPQTSPE YGQGINPISR LAQIQQAKKE 310 320 330 340 350 360 KEPEYTLLTE RGLPRRREFV MQVKVGNHTA EGTGTNKKVA KRNAAENMLE ILGFKVPQAQ 370 380 390 400 410 420 PTKPALKSEE KTPIKKPGDG RKVTFFEPGS GDENGTSNKE DEFRMPYLSH QQLPAGILPM 430 440 450 460 470 480 VPEVAQAVGV SQGHHTKDFT RAAPNPAKAT VTAMIARELL YGGTSPTAET ILKNNISSGH 490 500 510 520 530 540 VPHGPLTRPS EQLDYLSRVQ GFQVEYKDFP KNNKNEFVSL INCSSQPPLI SHGIGKDVES 550 560 570 CHDMAALNIL KLLSELDQQS TEMPRTGNGP MSVCGRC
	Isoform Short [UniParc]. Checksum: 509B6CF2B928C02C	FASTA	496	54,934
	10 20 30 40 50 60 MKLGKKPMYK PVDPYSRMQS TYNYNMRGGA YPPRYFYPFP VPPLLYQVEL SVGGQQFNGK 70 80 90 100 110 120 GKTRQAAKHD AAAKALRILQ NEPLPERLEV NGRESEEENL NKSEISQVFE IALKRNLPVN 130 140 150 160 170 180 FEVARESGPP HMKNFVTKVS VGEFVGEGEG KSKKISKKNA AIAVLEELKK LPPLPAVERV 190 200 210 220 230 240 KPRIKKKTKP IVKPQTSPEY GQGINPISRL AQIQQAKKEK EPEYTLLTER GLPRRREFVM 250 260 270 280 290 300 QVKVGNHTAE GTGTNKKVAK RNAAENMLEI LGFKVPQAQP TKPALKSEEK TPIKKPGDGR 310 320 330 340 350 360 KVTFFEPGSG DENGTSNKED EFRMPYLSHQ QLPAGILPMV PEVAQAVGVS QGHHTKDFTR 370 380 390 400 410 420 AAPNPAKATV TAMIARELLY GGTSPTAETI LKNNISSGHV PHGPLTRPSE QLDYLSRVQG 430 440 450 460 470 480 FQVEYKDFPK NNKNEFVSLI NCSSQPPLIS HGIGKDVESC HDMAALNILK LLSELDQQST 490 EMPRTGNGPM SVCGRC
	Isoform 3 [UniParc]. Checksum: 3AA2A672D9D87081	FASTA	502	55,635
	10 20 30 40 50 60 MKLGKKPMYK PVDPYSRMQS TYNYNMRGGA YPPRYFYPFP VPPLLYQVEL SVGGQQFNGK 70 80 90 100 110 120 GKTRQAAKHD AAAKALRILQ NEPLPERLEV NGRESEEENL NKSEISQVFE IALKRNLPVN 130 140 150 160 170 180 FESFPLKQVA RESGPPHMKN FVTKVSVGEF VGEGEGKSKK ISKKNAAIAV LEELKKLPPL 190 200 210 220 230 240 PAVERVKPRI KKKTKPIVKP QTSPEYGQGI NPISRLAQIQ QAKKEKEPEY TLLTERGLPR 250 260 270 280 290 300 RREFVMQVKV GNHTAEGTGT NKKVAKRNAA ENMLEILGFK VPQAQPTKPA LKSEEKTPIK 310 320 330 340 350 360 KPGDGRKVTF FEPGSGDENG TSNKEDEFRM PYLSHQQLPA GILPMVPEVA QAVGVSQGHH 370 380 390 400 410 420 TKDFTRAAPN PAKATVTAMI ARELLYGGTS PTAETILKNN ISSGHVPHGP LTRPSEQLDY 430 440 450 460 470 480 LSRVQGFQVE YKDFPKNNKN EFVSLINCSS QPPLISHGIG KDVESCHDMA ALNILKLLSE 490 500 LDQQSTEMPR TGNGPMSVCG RC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian Staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum." Wickham L., Duchaine T., Luo M., Nabi I.R., DesGroseillers L. Mol. Cell. Biol. 19:2220-2230(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Tissue: CNS.
[2]	"Interaction of influenza virus NS1 protein and the human homologue of Staufen in vivo and in vitro." Falcon A.M., Fortes P., Marion R.M., Beloso A., Ortin J. Nucleic Acids Res. 27:2241-2247(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Tissue: Kidney.
[3]	"Cloning and characterization of a splicing variant of human staufen protein." Mao Y., Xie Y., Jin F. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Trachea.
[6]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT). Tissue: Placenta and Skin.
[9]	"Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs." Weidensdorfer D., Stoehr N., Baude A., Lederer M., Koehn M., Schierhorn A., Buchmeier S., Wahle E., Huettelmaiery S. RNA 15:104-115(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MRNP COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF061939 mRNA. Translation: AAD17531.1.
AF061941 mRNA. Translation: AAD17533.1.
AF061938 mRNA. Translation: AAD17530.1.
AF061940 mRNA. Translation: AAD17532.1.
AJ132258 mRNA. Translation: CAB40082.1.
AY529074 mRNA. Translation: AAS76636.1.
AY546099 mRNA. Translation: AAS76637.1.
AL136601 mRNA. Translation: CAB66536.1.
AK292859 mRNA. Translation: BAF85548.1.
AL133174 Genomic DNA. Translation: CAC14084.1.
AL133174 Genomic DNA. Translation: CAC14085.1.
AL133174 Genomic DNA. Translation: CAI42820.1.
CH471077 Genomic DNA. Translation: EAW75669.1.
CH471077 Genomic DNA. Translation: EAW75671.1.
CH471077 Genomic DNA. Translation: EAW75672.1.
CH471077 Genomic DNA. Translation: EAW75673.1.
CH471077 Genomic DNA. Translation: EAW75674.1.
BC050432 mRNA. Translation: AAH50432.1.
BC095397 mRNA. Translation: AAH95397.1.

IPI

IPI00000001.
IPI00218609.
IPI00643664.

RefSeq

NP_001032405.1. NM_001037328.1.
NP_004593.2. NM_004602.2.
NP_059346.2. NM_017452.2.
NP_059347.2. NM_017453.2.
NP_059348.2. NM_017454.2.

UniGene

Hs.596704.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4DKK	X-ray	1.70	A	448-557	[»]

ProteinModelPortal

O95793.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95793. 53 interactions.

MINT

MINT-1160018.

STRING

9606.ENSP00000360922.

PTM databases

PhosphoSite

O95793.

Proteomic databases

PaxDb

O95793.

PRIDE

O95793.

Protocols and materials databases

DNASU

6780.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000340954; ENSP00000345425; ENSG00000124214.
ENST00000347458; ENSP00000323443; ENSG00000124214.
ENST00000360426; ENSP00000353604; ENSG00000124214.
ENST00000371802; ENSP00000360867; ENSG00000124214.
ENST00000371828; ENSP00000360893; ENSG00000124214.
ENST00000371856; ENSP00000360922; ENSG00000124214.

GeneID

6780.

KEGG

hsa:6780.

UCSC

uc002xua.3. human.
uc002xub.3. human.

Organism-specific databases

CTD

6780.

GeneCards

GC20M047729.

HGNC

HGNC:11370. STAU1.

HPA

CAB020839.

MIM

601716. gene.

neXtProt

NX_O95793.

PharmGKB

PA36188.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG299502.

HOGENOM

HOG000231025.

HOVERGEN

HBG005013.

InParanoid

O95793.

OMA

MKPRIKK.

OrthoDB

EOG418BN9.

PhylomeDB

O95793.

Gene expression databases

ArrayExpress

O95793.

Bgee

O95793.

CleanEx

HS_STAU1.

Genevestigator

O95793.

GermOnline

ENSG00000124214. Homo sapiens.

Family and domain databases

Gene3D

3.30.160.20. 3 hits.

InterPro

IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like_dom.
[Graphical view]

Pfam

PF00035. dsrm. 3 hits.
[Graphical view]

SMART

SM00358. DSRM. 3 hits.
[Graphical view]

PROSITE

PS50137. DS_RBD. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

STAU1. human.

GenomeRNAi

6780.

NextBio

26468.

SOURCE

Search...

Entry information

Entry name

STAU1_HUMAN

Accession

Primary (citable) accession number: O95793
Secondary accession number(s): A8K9Z4 , E1P5Y1, E1P608, Q5JW29, Q6GTM4, Q9H5B4, Q9H5B5, Q9Y3Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	March 7, 2006
Last modified:	May 29, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.