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Protein

U6 snRNA-associated Sm-like protein LSm8

Gene

LSM8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to the 3'-terminal U-tract of U6 snRNA and is probably a component of the spliceosome.

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • U6 snRNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
U6 snRNA-associated Sm-like protein LSm8
Gene namesi
Name:LSM8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:20471. LSM8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165618166.

Polymorphism and mutation databases

BioMutaiNAA38.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 9695U6 snRNA-associated Sm-like protein LSm8PRO_0000125582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine3 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO95777.
PaxDbiO95777.
PeptideAtlasiO95777.
PRIDEiO95777.

PTM databases

PhosphoSiteiO95777.

Expressioni

Gene expression databases

BgeeiO95777.
CleanExiHS_LSM8.
ExpressionAtlasiO95777. baseline and differential.
GenevisibleiO95777. HS.

Organism-specific databases

HPAiHPA020116.

Interactioni

Subunit structurei

LSm subunits form a heteromer with a doughnut shape.

Binary interactionsi

WithEntry#Exp.IntActNotes
LSM2Q9Y3336EBI-347779,EBI-347416
LSM3P623106EBI-347779,EBI-348239
LSM4Q9Y4Z04EBI-347779,EBI-372521

Protein-protein interaction databases

BioGridi119679. 61 interactions.
DIPiDIP-31195N.
IntActiO95777. 52 interactions.
MINTiMINT-1032595.
STRINGi9606.ENSP00000249299.

Structurei

3D structure databases

ProteinModelPortaliO95777.
SMRiO95777. Positions 2-74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the snRNP Sm proteins family.Curated

Phylogenomic databases

eggNOGiNOG235625.
GeneTreeiENSGT00730000111212.
HOVERGENiHBG105578.
InParanoidiO95777.
KOiK12627.
OMAiMAGYDQK.
OrthoDBiEOG7SV0XS.
PhylomeDBiO95777.
TreeFamiTF314555.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR001163. Ribonucl_LSM.
IPR006649. Ribonucl_LSM_euk/arc.
[Graphical view]
PfamiPF01423. LSM. 1 hit.
[Graphical view]
SMARTiSM00651. Sm. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSALENYIN RTVAVITSDG RMIVGTLKGF DQTINLILDE SHERVFSSSQ
60 70 80 90
GVEQVVLGLY IVRGDNVAVI GEIDEETDSA LDLGNIRAEP LNSVAH
Length:96
Mass (Da):10,403
Last modified:January 23, 2007 - v3
Checksum:i15D26E6B75AA2EE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182294 mRNA. Translation: AAD56232.1.
AC006389 Genomic DNA. Translation: AAD15542.1.
BC002742 mRNA. Translation: AAH02742.1.
BC022440 mRNA. Translation: AAH22440.1.
CCDSiCCDS5775.1.
RefSeqiNP_057284.1. NM_016200.4.
UniGeneiHs.655046.

Genome annotation databases

EnsembliENST00000249299; ENSP00000249299; ENSG00000128534.
GeneIDi51691.
KEGGihsa:51691.
UCSCiuc003vjg.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182294 mRNA. Translation: AAD56232.1.
AC006389 Genomic DNA. Translation: AAD15542.1.
BC002742 mRNA. Translation: AAH02742.1.
BC022440 mRNA. Translation: AAH22440.1.
CCDSiCCDS5775.1.
RefSeqiNP_057284.1. NM_016200.4.
UniGeneiHs.655046.

3D structure databases

ProteinModelPortaliO95777.
SMRiO95777. Positions 2-74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119679. 61 interactions.
DIPiDIP-31195N.
IntActiO95777. 52 interactions.
MINTiMINT-1032595.
STRINGi9606.ENSP00000249299.

PTM databases

PhosphoSiteiO95777.

Polymorphism and mutation databases

BioMutaiNAA38.

Proteomic databases

MaxQBiO95777.
PaxDbiO95777.
PeptideAtlasiO95777.
PRIDEiO95777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249299; ENSP00000249299; ENSG00000128534.
GeneIDi51691.
KEGGihsa:51691.
UCSCiuc003vjg.3. human.

Organism-specific databases

CTDi51691.
GeneCardsiGC07P117825.
HGNCiHGNC:20471. LSM8.
HPAiHPA020116.
MIMi607288. gene.
neXtProtiNX_O95777.
PharmGKBiPA165618166.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG235625.
GeneTreeiENSGT00730000111212.
HOVERGENiHBG105578.
InParanoidiO95777.
KOiK12627.
OMAiMAGYDQK.
OrthoDBiEOG7SV0XS.
PhylomeDBiO95777.
TreeFamiTF314555.

Miscellaneous databases

GeneWikiiLSM8.
GenomeRNAii51691.
NextBioi55698.
PROiO95777.
SOURCEiSearch...

Gene expression databases

BgeeiO95777.
CleanExiHS_LSM8.
ExpressionAtlasiO95777. baseline and differential.
GenevisibleiO95777. HS.

Family and domain databases

InterProiIPR010920. LSM_dom.
IPR001163. Ribonucl_LSM.
IPR006649. Ribonucl_LSM_euk/arc.
[Graphical view]
PfamiPF01423. LSM. 1 hit.
[Graphical view]
SMARTiSM00651. Sm. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro."
    Achsel T., Brahms H., Kastner B., Bachi A., Wilm M., Luehrmann R.
    EMBO J. 18:5789-5802(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Uterus.
  4. Bienvenut W.V.
    Submitted (JUL-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-11; 29-44 AND 88-96, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiLSM8_HUMAN
AccessioniPrimary (citable) accession number: O95777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.