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O95754 (SEM4F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Semaphorin-4F
Alternative name(s):
Semaphorin-M
Short name=Sema M
Semaphorin-W
Short name=Sema W
Gene names
Name:SEMA4F
Synonyms:SEMAM, SEMAW
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Has growth cone collapse activity against retinal ganglion-cell axons By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 PSI domain.

Contains 1 Sema domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: O95754-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: O95754-2)

The sequence of this isoform differs from the canonical sequence as follows:
     120-274: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Potential
Chain35 – 770736Semaphorin-4F
PRO_0000032330

Regions

Topological domain35 – 659625Extracellular Potential
Transmembrane660 – 68021Helical; Potential
Topological domain681 – 77090Cytoplasmic Potential
Domain42 – 510469Sema
Domain512 – 56352PSI
Domain580 – 63556Ig-like C2-type

Amino acid modifications

Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation1331N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Disulfide bond112 ↔ 122 By similarity
Disulfide bond140 ↔ 149 By similarity
Disulfide bond273 ↔ 384 By similarity
Disulfide bond297 ↔ 343 By similarity
Disulfide bond513 ↔ 530 By similarity
Disulfide bond522 ↔ 539 By similarity
Disulfide bond587 ↔ 628 By similarity

Natural variations

Alternative sequence120 – 274155Missing in isoform Short.
VSP_006043

Experimental info

Sequence conflict5331S → N in BAA75631. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: CFBB74B41DF0E9C8

FASTA77083,511
        10         20         30         40         50         60 
MPASAARPRP GPGQPTASPF PLLLLAVLSG PVSGRVPRSV PRTSLPISEA DSCLTRFAVP 

        70         80         90        100        110        120 
HTYNYSVLLV DPASHTLYVG ARDTIFALSL PFSGERPRRI DWMVPEAHRQ NCRKKGKKED 

       130        140        150        160        170        180 
ECHNFVQILA IANASHLLTC GTFAFDPKCG VIDVSRFQQV ERLESGRGKC PFEPAQRSAA 

       190        200        210        220        230        240 
VMAGGVLYAA TVKNYLGTEP IITRAVGRAE DWIRTDTLPS WLNAPAFVAA VALSPAEWGD 

       250        260        270        280        290        300 
EDGDDEIYFF FTETSRAFDS YERIKVPRVA RVCAGDLGGR KTLQQRWTTF LKADLLCPGP 

       310        320        330        340        350        360 
EHGRASSVLQ DVAVLRPELG AGTPIFYGIF SSQWEGATIS AVCAFRPQDI RTVLNGPFRE 

       370        380        390        400        410        420 
LKHDCNRGLP VVDNDVPQPR PGECITNNMK LRHFGSSLSL PDRVLTFIRD HPLMDRPVFP 

       430        440        450        460        470        480 
ADGHPLLVTT DTAYLRVVAH RVTSLSGKEY DVLYLGTEDG HLHRAVRIGA QLSVLEDLAL 

       490        500        510        520        530        540 
FPEPQPVENM KLYHSWLLVG SRTEVTQVNT TNCGRLQSCS ECILAQDPVC AWSFRLDECV 

       550        560        570        580        590        600 
AHAGEHRGLV QDIESADVSS LCPKEPGERP VVFEVPVATA AHVVLPCSPS SAWASCVWHQ 

       610        620        630        640        650        660 
PSGVTALTPR RDGLEVVVTP GAMGAYACEC QEGGAAHVVA AYSLVWGSQR DAPSRAHTVG 

       670        680        690        700        710        720 
AGLAGFFLGI LAASLTLILI GRRQQRRRQR ELLARDKVGL DLGAPPSGTT SYSQDPPSPS 

       730        740        750        760        770 
PEDERLPLAL AKRGSGFGGF SPPFLLDPCP SPAHIRLTGA PLATCDETSI 

« Hide

Isoform Short [UniParc].

Checksum: 43FBD465AFC6AEAD
Show »

FASTA61566,477

References

« Hide 'large scale' references
[1]"Cloning, expression, and genetic mapping of Sema W, a member of the semaphorin family."
Encinas J.A., Kikuchi K., Chedotal A., de Castro F., Goodman C.S., Kimura T.
Proc. Natl. Acad. Sci. U.S.A. 96:2491-2496(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Brain.
[2]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Amygdala.
[3]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT).
Tissue: Brain.
[6]"Large-scale comparative sequence analysis of human and mouse genomic DNA in the mnd2 region of mouse chromosome 6 reveals coding regions of three new genes."
Jang W., Spilson S.V., Hua A., Roe B., Meisler M.H.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 347-770.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB022317 mRNA. Translation: BAA75631.1.
AL136552 mRNA. Translation: CAB66487.1.
AK075384 mRNA. Translation: BAC11584.1.
AC006544 Genomic DNA. No translation available.
BC018361 mRNA. Translation: AAH18361.1.
BC038411 mRNA. Translation: AAH38411.1.
AF053369 mRNA. Translation: AAF80660.1.
CCDSCCDS1955.1. [O95754-1]
CCDS62942.1. [O95754-2]
RefSeqNP_001258590.1. NM_001271661.1. [O95754-2]
NP_001258591.1. NM_001271662.1.
NP_004254.2. NM_004263.4. [O95754-1]
UniGeneHs.25887.

3D structure databases

ProteinModelPortalO95754.
SMRO95754. Positions 40-632.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-3003200.
STRING9606.ENSP00000350547.

PTM databases

PhosphoSiteO95754.

Proteomic databases

PaxDbO95754.
PRIDEO95754.

Protocols and materials databases

DNASU10505.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339773; ENSP00000342675; ENSG00000135622. [O95754-2]
ENST00000357877; ENSP00000350547; ENSG00000135622. [O95754-1]
GeneID10505.
KEGGhsa:10505.
UCSCuc002sna.2. human. [O95754-1]
uc002snb.2. human. [O95754-2]

Organism-specific databases

CTD10505.
GeneCardsGC02P074793.
HGNCHGNC:10734. SEMA4F.
MIM603706. gene.
neXtProtNX_O95754.
PharmGKBPA35656.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG258001.
HOGENOMHOG000116087.
HOVERGENHBG093234.
InParanoidO95754.
KOK06521.
OMACNRGLPV.
OrthoDBEOG7KM5S4.
PhylomeDBO95754.
TreeFamTF352903.

Gene expression databases

ArrayExpressO95754.
BgeeO95754.
CleanExHS_SEMA4F.
GenevestigatorO95754.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR015512. Seamphorin_4F.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERPTHR11036. PTHR11036. 1 hit.
PTHR11036:SF72. PTHR11036:SF72. 1 hit.
PfamPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTSM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEPS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSEMA4F.
GenomeRNAi10505.
NextBio39852.
PROO95754.
SOURCESearch...

Entry information

Entry nameSEM4F_HUMAN
AccessionPrimary (citable) accession number: O95754
Secondary accession number(s): Q542Y7, Q9NS35
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM