O95754 (SEM4F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Semaphorin-4F

Alternative name(s):
Semaphorin-M
Short name=Sema M
Semaphorin-W
Short name=Sema W

Gene names

Name:	SEMA4F
Synonyms:	SEMAM, SEMAW

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has growth cone collapse activity against retinal ganglion-cell axons By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Traceable author statement Ref.1. Source: ProtInc cell-cell signaling Traceable author statement Ref.1. Source: ProtInc negative regulation of axon extension Inferred from electronic annotation. Source: Compara retinal ganglion cell axon guidance Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from direct assay. Source: LIFEdb integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc postsynaptic membrane Inferred from electronic annotation. Source: Compara
Molecular_function	receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: O95754-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O95754-2)

The sequence of this isoform differs from the canonical sequence as follows:
     120-274: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 770	736	Semaphorin-4F		PRO_0000032330
Regions
	Topological domain	35 – 659	625	Extracellular Potential
	Transmembrane	660 – 680	21	Helical; Potential
	Topological domain	681 – 770	90	Cytoplasmic Potential
	Domain	42 – 510	469	Sema
	Domain	512 – 563	52	PSI
	Domain	580 – 635	56	Ig-like C2-type
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	509	1	N-linked (GlcNAc...) Potential
	Disulfide bond	112 ↔ 122		By similarity
	Disulfide bond	140 ↔ 149		By similarity
	Disulfide bond	273 ↔ 384		By similarity
	Disulfide bond	297 ↔ 343		By similarity
	Disulfide bond	513 ↔ 530		By similarity
	Disulfide bond	522 ↔ 539		By similarity
	Disulfide bond	587 ↔ 628		By similarity
Natural variations
	Alternative sequence	120 – 274	155	Missing in isoform Short.		VSP_006043
Experimental info
	Sequence conflict	533	1	S → N in BAA75631. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified May 30, 2000. Version 2. Checksum: CFBB74B41DF0E9C8	FASTA	770	83,511
	10 20 30 40 50 60 MPASAARPRP GPGQPTASPF PLLLLAVLSG PVSGRVPRSV PRTSLPISEA DSCLTRFAVP 70 80 90 100 110 120 HTYNYSVLLV DPASHTLYVG ARDTIFALSL PFSGERPRRI DWMVPEAHRQ NCRKKGKKED 130 140 150 160 170 180 ECHNFVQILA IANASHLLTC GTFAFDPKCG VIDVSRFQQV ERLESGRGKC PFEPAQRSAA 190 200 210 220 230 240 VMAGGVLYAA TVKNYLGTEP IITRAVGRAE DWIRTDTLPS WLNAPAFVAA VALSPAEWGD 250 260 270 280 290 300 EDGDDEIYFF FTETSRAFDS YERIKVPRVA RVCAGDLGGR KTLQQRWTTF LKADLLCPGP 310 320 330 340 350 360 EHGRASSVLQ DVAVLRPELG AGTPIFYGIF SSQWEGATIS AVCAFRPQDI RTVLNGPFRE 370 380 390 400 410 420 LKHDCNRGLP VVDNDVPQPR PGECITNNMK LRHFGSSLSL PDRVLTFIRD HPLMDRPVFP 430 440 450 460 470 480 ADGHPLLVTT DTAYLRVVAH RVTSLSGKEY DVLYLGTEDG HLHRAVRIGA QLSVLEDLAL 490 500 510 520 530 540 FPEPQPVENM KLYHSWLLVG SRTEVTQVNT TNCGRLQSCS ECILAQDPVC AWSFRLDECV 550 560 570 580 590 600 AHAGEHRGLV QDIESADVSS LCPKEPGERP VVFEVPVATA AHVVLPCSPS SAWASCVWHQ 610 620 630 640 650 660 PSGVTALTPR RDGLEVVVTP GAMGAYACEC QEGGAAHVVA AYSLVWGSQR DAPSRAHTVG 670 680 690 700 710 720 AGLAGFFLGI LAASLTLILI GRRQQRRRQR ELLARDKVGL DLGAPPSGTT SYSQDPPSPS 730 740 750 760 770 PEDERLPLAL AKRGSGFGGF SPPFLLDPCP SPAHIRLTGA PLATCDETSI
	Isoform Short [UniParc]. Checksum: 43FBD465AFC6AEAD	FASTA	615	66,477
	10 20 30 40 50 60 MPASAARPRP GPGQPTASPF PLLLLAVLSG PVSGRVPRSV PRTSLPISEA DSCLTRFAVP 70 80 90 100 110 120 HTYNYSVLLV DPASHTLYVG ARDTIFALSL PFSGERPRRI DWMVPEAHRQ NCRKKGKKEG 130 140 150 160 170 180 DLGGRKTLQQ RWTTFLKADL LCPGPEHGRA SSVLQDVAVL RPELGAGTPI FYGIFSSQWE 190 200 210 220 230 240 GATISAVCAF RPQDIRTVLN GPFRELKHDC NRGLPVVDND VPQPRPGECI TNNMKLRHFG 250 260 270 280 290 300 SSLSLPDRVL TFIRDHPLMD RPVFPADGHP LLVTTDTAYL RVVAHRVTSL SGKEYDVLYL 310 320 330 340 350 360 GTEDGHLHRA VRIGAQLSVL EDLALFPEPQ PVENMKLYHS WLLVGSRTEV TQVNTTNCGR 370 380 390 400 410 420 LQSCSECILA QDPVCAWSFR LDECVAHAGE HRGLVQDIES ADVSSLCPKE PGERPVVFEV 430 440 450 460 470 480 PVATAAHVVL PCSPSSAWAS CVWHQPSGVT ALTPRRDGLE VVVTPGAMGA YACECQEGGA 490 500 510 520 530 540 AHVVAAYSLV WGSQRDAPSR AHTVGAGLAG FFLGILAASL TLILIGRRQQ RRRQRELLAR 550 560 570 580 590 600 DKVGLDLGAP PSGTTSYSQD PPSPSPEDER LPLALAKRGS GFGGFSPPFL LDPCPSPAHI 610 RLTGAPLATC DETSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and genetic mapping of Sema W, a member of the semaphorin family." Encinas J.A., Kikuchi K., Chedotal A., de Castro F., Goodman C.S., Kimura T. Proc. Natl. Acad. Sci. U.S.A. 96:2491-2496(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Tissue: Brain.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Amygdala.
[3]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LONG AND SHORT). Tissue: Brain.
[6]	"Large-scale comparative sequence analysis of human and mouse genomic DNA in the mnd2 region of mouse chromosome 6 reveals coding regions of three new genes." Jang W., Spilson S.V., Hua A., Roe B., Meisler M.H. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 347-770.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022317 mRNA. Translation: BAA75631.1. AL136552 mRNA. Translation: CAB66487.1. AK075384 mRNA. Translation: BAC11584.1. AC006544 Genomic DNA. No translation available. BC018361 mRNA. Translation: AAH18361.1. BC038411 mRNA. Translation: AAH38411.1. AF053369 mRNA. Translation: AAF80660.1.
IPI	IPI00032914. IPI00216574.
RefSeq	NP_001258590.1. NM_001271661.1. NP_001258591.1. NM_001271662.1. NP_004254.2. NM_004263.4.
UniGene	Hs.25887.
3D structure databases
ProteinModelPortal	O95754.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000350547.
PTM databases
PhosphoSite	O95754.
Proteomic databases
PaxDb	O95754.
PRIDE	O95754.
Protocols and materials databases
DNASU	10505.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000339773; ENSP00000342675; ENSG00000135622. ENST00000357877; ENSP00000350547; ENSG00000135622.
GeneID	10505.
KEGG	hsa:10505.
UCSC	uc002sna.1. human. uc002snb.1. human.
Organism-specific databases
CTD	10505.
GeneCards	GC02P074793.
HGNC	HGNC:10734. SEMA4F.
MIM	603706. gene.
neXtProt	NX_O95754.
PharmGKB	PA35656.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG258001.
HOGENOM	HOG000116087.
HOVERGEN	HBG093234.
InParanoid	O95754.
KO	K06521.
OMA	CNRGLPV.
OrthoDB	EOG4J117K.
PhylomeDB	O95754.
Gene expression databases
ArrayExpress	O95754.
Bgee	O95754.
CleanEx	HS_SEMA4F.
Genevestigator	O95754.
GermOnline	ENSG00000135622. Homo sapiens.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR003659. Plexin-like. IPR016201. Plexin-like_fold. IPR002165. Plexin_repeat. IPR015512. Seamphorin_4F. IPR027231. Semaphorin. IPR001627. Semaphorin/CD100_Ag. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
PANTHER	PTHR11036. PTHR11036. 1 hit. PTHR11036:SF13. PTHR11036:SF13. 1 hit.
Pfam	PF01437. PSI. 1 hit. PF01403. Sema. 1 hit. [Graphical view]
SMART	SM00423. PSI. 1 hit. SM00630. Sema. 1 hit. [Graphical view]
SUPFAM	SSF103575. Plexin-like_fold. 1 hit. SSF101912. Sema. 1 hit.
PROSITE	PS51004. SEMA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	10505.
NextBio	39852.
SOURCE	Search...

Entry information

Entry name

SEM4F_HUMAN

Accession

Primary (citable) accession number: O95754
Secondary accession number(s): Q542Y7, Q9NS35

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.