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Protein

Cytotoxic and regulatory T-cell molecule

Gene

CRTAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.HMP:1 Publication

GO - Molecular functioni

  • cell adhesion molecule binding Source: GO_Central
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: GO_Central
  • receptor activity Source: GO_Central
  • receptor binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxic and regulatory T-cell molecule
Alternative name(s):
Class-I MHC-restricted T-cell-associated molecule
CD_antigen: CD355
Gene namesi
Name:CRTAMTAS:
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000109943.8.
HGNCiHGNC:24313. CRTAM.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 287ExtracellularIEP:Add BLAST270
Transmembranei288 – 308HelicalIEP:Add BLAST21
Topological domaini309 – 393CytoplasmicIEP:Add BLAST85

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi56253.
OpenTargetsiENSG00000109943.
PharmGKBiPA145149072.

Polymorphism and mutation databases

BioMutaiCRTAM.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17IEP:Add BLAST17
ChainiPRO_000029260218 – 393Cytotoxic and regulatory T-cell moleculeAdd BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...) asparagineIEP:1
Disulfide bondi38 ↔ 98IEP:1 Publication
Glycosylationi87N-linked (GlcNAc...) asparagineIEP:1
Disulfide bondi141 ↔ 196IEP:
Glycosylationi178N-linked (GlcNAc...) asparagineIEP:1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO95727.
PeptideAtlasiO95727.
PRIDEiO95727.

PTM databases

iPTMnetiO95727.
PhosphoSitePlusiO95727.

Expressioni

Tissue specificityi

In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.3 Publications

Gene expression databases

BgeeiENSG00000109943.
CleanExiHS_CRTAM.
GenevisibleiO95727. HS.

Organism-specific databases

HPAiHPA054595.

Interactioni

Subunit structurei

Interacts with CADM1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CADM1Q9BY674EBI-16044697,EBI-5652260

GO - Molecular functioni

  • cell adhesion molecule binding Source: GO_Central
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: GO_Central
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-60155N.
IntActiO95727. 1 interactor.
STRINGi9606.ENSP00000227348.

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 29ND:6
Beta strandi34 – 39ND:6
Beta strandi47 – 51ND:5
Beta strandi57 – 60ND:4
Beta strandi72 – 77ND:6
Beta strandi80 – 85ND:6
Helixi90 – 92ND:3
Beta strandi94 – 115ND:22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RBGX-ray2.30A/B/C/D18-117[»]
4H5SX-ray1.70A18-117[»]
ProteinModelPortaliO95727.
SMRiO95727.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95727.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 114Ig-like V-typeIEP:Add BLAST97
Domaini118 – 210Ig-like C2-typeIEP:Add BLAST93

Sequence similaritiesi

Belongs to the nectin family.1 Publication

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IESB. Eukaryota.
ENOG4111KGD. LUCA.
GeneTreeiENSGT00900000140811.
HOGENOMiHOG000008690.
HOVERGENiHBG053014.
InParanoidiO95727.
KOiK16361.
OMAiHVIWKKE.
OrthoDBiEOG091G0BHE.
PhylomeDBiO95727.
TreeFamiTF326804.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiView protein in InterPro
IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
PfamiView protein in Pfam
PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 1 hit.
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: O95727-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW
60 70 80 90 100
LTPSGFTIFL NEYPALKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH
110 120 130 140 150
YSDSVSTKEV KVIVLATPFK PILEASVIRK QNGEEHVVLM CSTMRSKPPP
160 170 180 190 200
QITWLLGNSM EVSGGTLHEF ETDGKKCNTT STLIIHTYGK NSTVDCIIRH
210 220 230 240 250
RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ PTSTVSVTED
260 270 280 290 300
SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI
310 320 330 340 350
LFIIVQLFIM KLRKAHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ
360 370 380 390
SSHPMRCMNY ITKLYSEAKT KRKENVQHSK LEEKHIQVPE SIV
Length:393
Mass (Da):44,641
Last modified:June 26, 2007 - v2
Checksum:iCB8173032EE45F03
GO
Isoform 2 (identifier: O95727-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     200-216: HRGLQGRKLVAPFRFED → MWVKLLSIVAEFCFSPF

Note: No experimental confirmation available.IKR:
Show »
Length:194
Mass (Da):22,256
Checksum:i2CD6A68172F93C8B
GO

Sequence cautioni

The sequence BAD93067 differs from that shown. Reason: Erroneous initiation.IKR:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65A → V in AAH70266 (PubMed:15489334).IKR:1
Sequence conflicti315A → T in AAH70266 (PubMed:15489334).IKR:1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04986816E → A. Corresponds to variant dbSNP:rs35411582Ensembl.1
Natural variantiVAR_04986978A → D. Corresponds to variant dbSNP:rs34397316Ensembl.1
Natural variantiVAR_049870173D → G. Corresponds to variant dbSNP:rs35136295Ensembl.1
Natural variantiVAR_032999321K → R1 PublicationCorresponds to variant dbSNP:rs2272094Ensembl.1
Natural variantiVAR_033000368A → G1 PublicationCorresponds to variant dbSNP:rs1916036Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0524711 – 199Missing in isoform 2. IBD:Add BLAST199
Alternative sequenceiVSP_052472200 – 216HRGLQ…FRFED → MWVKLLSIVAEFCFSPF in isoform 2. IBD:Add BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001622 mRNA. Translation: AAC80267.1.
AB209830 mRNA. Translation: BAD93067.1. Different initiation.
BC070266 mRNA. Translation: AAH70266.1.
CCDSiCCDS76489.1. [O95727-2]
CCDS8437.1. [O95727-1]
RefSeqiNP_001291711.1. NM_001304782.1. [O95727-2]
NP_062550.2. NM_019604.3. [O95727-1]
UniGeneiHs.159523.

Genome annotation databases

EnsembliENST00000227348; ENSP00000227348; ENSG00000109943. [O95727-1]
ENST00000533709; ENSP00000433728; ENSG00000109943. [O95727-2]
GeneIDi56253.
KEGGihsa:56253.
UCSCiuc001pyj.4. human. [O95727-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCRTAM_HUMAN
AccessioniPrimary (citable) accession number: O95727
Secondary accession number(s): Q59EI1, Q6IRX2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: October 25, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families