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O95727

- CRTAM_HUMAN

UniProt

O95727 - CRTAM_HUMAN

Protein

Cytotoxic and regulatory T-cell molecule

Gene

CRTAM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (26 Jun 2007)
      Previous versions | rss
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    Functioni

    Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.By similarity1 Publication

    GO - Molecular functioni

    1. receptor binding Source: UniProtKB

    GO - Biological processi

    1. cell recognition Source: HGNC
    2. detection of stimulus Source: HGNC
    3. detection of tumor cell Source: HGNC
    4. immune system process Source: UniProtKB-KW
    5. positive regulation of cytokine secretion Source: HGNC
    6. positive regulation of natural killer cell mediated cytotoxicity Source: HGNC
    7. positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: HGNC
    8. regulation of immune response Source: Reactome

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytotoxic and regulatory T-cell molecule
    Alternative name(s):
    Class-I MHC-restricted T-cell-associated molecule
    CD_antigen: CD355
    Gene namesi
    Name:CRTAMImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:24313. CRTAM.

    Subcellular locationi

    Membrane Sequence Analysis; Single-pass type I membrane protein Sequence Analysis

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: HGNC

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA145149072.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 393376Cytotoxic and regulatory T-cell moleculePRO_0000292602Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi38 ↔ 981 PublicationPROSITE-ProRule annotation
    Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi141 ↔ 196PROSITE-ProRule annotation
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO95727.
    PRIDEiO95727.

    Expressioni

    Tissue specificityi

    In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.3 Publications

    Gene expression databases

    BgeeiO95727.
    CleanExiHS_CRTAM.
    GenevestigatoriO95727.

    Organism-specific databases

    HPAiHPA054595.

    Interactioni

    Subunit structurei

    Interacts with CADM1.2 Publications

    Protein-protein interaction databases

    DIPiDIP-60155N.
    STRINGi9606.ENSP00000227348.

    Structurei

    Secondary structure

    1
    393
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi24 – 296
    Beta strandi34 – 396
    Beta strandi47 – 515
    Beta strandi57 – 604
    Beta strandi72 – 776
    Beta strandi80 – 856
    Helixi90 – 923
    Beta strandi94 – 11522

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RBGX-ray2.30A/B/C/D18-117[»]
    4H5SX-ray1.70A18-117[»]
    ProteinModelPortaliO95727.
    SMRiO95727. Positions 22-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95727.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini18 – 287270ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini309 – 39385CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 11497Ig-like V-typeSequence AnalysisAdd
    BLAST
    Domaini118 – 21093Ig-like C2-typeSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the nectin family.1 Publication
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.Sequence Analysis

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72056.
    HOGENOMiHOG000008690.
    HOVERGENiHBG053014.
    InParanoidiO95727.
    KOiK16361.
    OMAiHVIWKKE.
    OrthoDBiEOG7PVWPD.
    PhylomeDBiO95727.
    TreeFamiTF326804.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: O95727-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW    50
    LTPSGFTIFL NEYPALKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH 100
    YSDSVSTKEV KVIVLATPFK PILEASVIRK QNGEEHVVLM CSTMRSKPPP 150
    QITWLLGNSM EVSGGTLHEF ETDGKKCNTT STLIIHTYGK NSTVDCIIRH 200
    RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ PTSTVSVTED 250
    SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI 300
    LFIIVQLFIM KLRKAHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ 350
    SSHPMRCMNY ITKLYSEAKT KRKENVQHSK LEEKHIQVPE SIV 393
    Length:393
    Mass (Da):44,641
    Last modified:June 26, 2007 - v2
    Checksum:iCB8173032EE45F03
    GO
    Isoform 2 (identifier: O95727-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-199: Missing.
         200-216: HRGLQGRKLVAPFRFED → MWVKLLSIVAEFCFSPF

    Note: No experimental confirmation available.Curated

    Show »
    Length:194
    Mass (Da):22,256
    Checksum:i2CD6A68172F93C8B
    GO

    Sequence cautioni

    The sequence BAD93067.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651A → V in AAH70266. (PubMed:15489334)Curated
    Sequence conflicti315 – 3151A → T in AAH70266. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti16 – 161E → A.
    Corresponds to variant rs35411582 [ dbSNP | Ensembl ].
    VAR_049868
    Natural varianti78 – 781A → D.
    Corresponds to variant rs34397316 [ dbSNP | Ensembl ].
    VAR_049869
    Natural varianti173 – 1731D → G.
    Corresponds to variant rs35136295 [ dbSNP | Ensembl ].
    VAR_049870
    Natural varianti321 – 3211K → R.1 Publication
    Corresponds to variant rs2272094 [ dbSNP | Ensembl ].
    VAR_032999
    Natural varianti368 – 3681A → G.1 Publication
    Corresponds to variant rs1916036 [ dbSNP | Ensembl ].
    VAR_033000

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 199199Missing in isoform 2. 1 PublicationVSP_052471Add
    BLAST
    Alternative sequencei200 – 21617HRGLQ…FRFED → MWVKLLSIVAEFCFSPF in isoform 2. 1 PublicationVSP_052472Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001622 mRNA. Translation: AAC80267.1.
    AB209830 mRNA. Translation: BAD93067.1. Different initiation.
    BC070266 mRNA. Translation: AAH70266.1.
    CCDSiCCDS8437.1. [O95727-1]
    RefSeqiNP_062550.2. NM_019604.2. [O95727-1]
    UniGeneiHs.159523.

    Genome annotation databases

    EnsembliENST00000227348; ENSP00000227348; ENSG00000109943. [O95727-1]
    ENST00000533709; ENSP00000433728; ENSG00000109943. [O95727-2]
    GeneIDi56253.
    KEGGihsa:56253.
    UCSCiuc001pyj.3. human. [O95727-1]
    uc001pyk.3. human. [O95727-2]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF001622 mRNA. Translation: AAC80267.1 .
    AB209830 mRNA. Translation: BAD93067.1 . Different initiation.
    BC070266 mRNA. Translation: AAH70266.1 .
    CCDSi CCDS8437.1. [O95727-1 ]
    RefSeqi NP_062550.2. NM_019604.2. [O95727-1 ]
    UniGenei Hs.159523.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RBG X-ray 2.30 A/B/C/D 18-117 [» ]
    4H5S X-ray 1.70 A 18-117 [» ]
    ProteinModelPortali O95727.
    SMRi O95727. Positions 22-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60155N.
    STRINGi 9606.ENSP00000227348.

    Proteomic databases

    PaxDbi O95727.
    PRIDEi O95727.

    Protocols and materials databases

    DNASUi 56253.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000227348 ; ENSP00000227348 ; ENSG00000109943 . [O95727-1 ]
    ENST00000533709 ; ENSP00000433728 ; ENSG00000109943 . [O95727-2 ]
    GeneIDi 56253.
    KEGGi hsa:56253.
    UCSCi uc001pyj.3. human. [O95727-1 ]
    uc001pyk.3. human. [O95727-2 ]

    Organism-specific databases

    CTDi 56253.
    GeneCardsi GC11P122743.
    H-InvDB HIX0035844.
    HGNCi HGNC:24313. CRTAM.
    HPAi HPA054595.
    MIMi 612597. gene.
    neXtProti NX_O95727.
    PharmGKBi PA145149072.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG72056.
    HOGENOMi HOG000008690.
    HOVERGENi HBG053014.
    InParanoidi O95727.
    KOi K16361.
    OMAi HVIWKKE.
    OrthoDBi EOG7PVWPD.
    PhylomeDBi O95727.
    TreeFami TF326804.

    Enzyme and pathway databases

    Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Miscellaneous databases

    EvolutionaryTracei O95727.
    GenomeRNAii 56253.
    NextBioi 61889.
    PROi O95727.
    SOURCEi Search...

    Gene expression databases

    Bgeei O95727.
    CleanExi HS_CRTAM.
    Genevestigatori O95727.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM)."
      Kennedy J., Vicari A.P., Saylor V., Zurawski S.M., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A.
      J. Leukoc. Biol. 67:725-734(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT ARG-321.
    2. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: BrainImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-368.
      Tissue: Peripheral bloodImported.
    4. "The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM."
      Boles K.S., Barchet W., Diacovo T., Cella M., Colonna M.
      Blood 106:779-786(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CADM1, TISSUE SPECIFICITY.
    5. "Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule."
      Galibert L., Diemer G.S., Liu Z., Johnson R.S., Smith J.L., Walzer T., Comeau M.R., Rauch C.T., Wolfson M.F., Sorensen R.A., Van der Vuurst de Vries A.-R., Branstetter D.G., Koelling R.M., Scholler J., Fanslow W.C., Baum P.R., Derry J.M., Yan W.
      J. Biol. Chem. 280:21955-21964(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CADM1.
    6. "Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes."
      Patino-Lopez G., Hevezi P., Lee J., Willhite D., Verge G.M., Lechner S.M., Ortiz-Navarrete V., Zlotnik A.
      J. Neuroimmunol. 171:145-155(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Structure analysis of human class-I MHC restricted T-cell associated molecule."
      New York structural genomics research consortium (NYSGRC)
      Submitted (MAY-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 18-117, DISULFIDE BOND.

    Entry informationi

    Entry nameiCRTAM_HUMAN
    AccessioniPrimary (citable) accession number: O95727
    Secondary accession number(s): Q59EI1, Q6IRX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: June 26, 2007
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3