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O95727 (CRTAM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytotoxic and regulatory T-cell molecule
Alternative name(s):
Class-I MHC-restricted T-cell-associated molecule
CD_antigen=CD355
Gene names
Name:CRTAM
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Ref.4 UniProtKB Q149L7

Subunit structure

Interacts with CADM1. Ref.4 Ref.5

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. Ref.1 Ref.4 Ref.6

Sequence similarities

Belongs to the nectin family. Ref.6

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution

The sequence BAD93067.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: O95727-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.2 (identifier: O95727-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     200-216: HRGLQGRKLVAPFRFED → MWVKLLSIVAEFCFSPF
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 393376Cytotoxic and regulatory T-cell molecule
PRO_0000292602

Regions

Topological domain18 – 287270Extracellular Potential
Transmembrane288 – 30821Helical; Potential
Topological domain309 – 39385Cytoplasmic Potential
Domain18 – 11497Ig-like V-type
Domain118 – 21093Ig-like C2-type

Amino acid modifications

Glycosylation211N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Disulfide bond38 ↔ 98 Ref.7
Disulfide bond141 ↔ 196 By similarity

Natural variations

Alternative sequence1 – 199199Missing in isoform 2. Ref.2
VSP_052471
Alternative sequence200 – 21617HRGLQ…FRFED → MWVKLLSIVAEFCFSPF in isoform 2. Ref.2
VSP_052472
Natural variant161E → A.
Corresponds to variant rs35411582 [ dbSNP | Ensembl ].
VAR_049868
Natural variant781A → D.
Corresponds to variant rs34397316 [ dbSNP | Ensembl ].
VAR_049869
Natural variant1731D → G.
Corresponds to variant rs35136295 [ dbSNP | Ensembl ].
VAR_049870
Natural variant3211K → R. Ref.1
Corresponds to variant rs2272094 [ dbSNP | Ensembl ].
VAR_032999
Natural variant3681A → G. Ref.3
Corresponds to variant rs1916036 [ dbSNP | Ensembl ].
VAR_033000

Experimental info

Sequence conflict651A → V in AAH70266. Ref.3
Sequence conflict3151A → T in AAH70266. Ref.3

Secondary structure

................. 393
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 26, 2007. Version 2.
Checksum: CB8173032EE45F03

FASTA39344,641
        10         20         30         40         50         60 
MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW LTPSGFTIFL 

        70         80         90        100        110        120 
NEYPALKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH YSDSVSTKEV KVIVLATPFK 

       130        140        150        160        170        180 
PILEASVIRK QNGEEHVVLM CSTMRSKPPP QITWLLGNSM EVSGGTLHEF ETDGKKCNTT 

       190        200        210        220        230        240 
STLIIHTYGK NSTVDCIIRH RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ 

       250        260        270        280        290        300 
PTSTVSVTED SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI 

       310        320        330        340        350        360 
LFIIVQLFIM KLRKAHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ SSHPMRCMNY 

       370        380        390 
ITKLYSEAKT KRKENVQHSK LEEKHIQVPE SIV 

« Hide

Isoform 2 [UniParc].

Checksum: 2CD6A68172F93C8B
Show »

FASTA19422,256

References

« Hide 'large scale' references
[1]"A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM)."
Kennedy J., Vicari A.P., Saylor V., Zurawski S.M., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A.
J. Leukoc. Biol. 67:725-734(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT ARG-321.
[2]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-368.
Tissue: Peripheral blood.
[4]"The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM."
Boles K.S., Barchet W., Diacovo T., Cella M., Colonna M.
Blood 106:779-786(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CADM1, TISSUE SPECIFICITY.
[5]"Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule."
Galibert L., Diemer G.S., Liu Z., Johnson R.S., Smith J.L., Walzer T., Comeau M.R., Rauch C.T., Wolfson M.F., Sorensen R.A., Van der Vuurst de Vries A.-R., Branstetter D.G., Koelling R.M., Scholler J., Fanslow W.C., Baum P.R., Derry J.M., Yan W.
J. Biol. Chem. 280:21955-21964(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CADM1.
[6]"Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes."
Patino-Lopez G., Hevezi P., Lee J., Willhite D., Verge G.M., Lechner S.M., Ortiz-Navarrete V., Zlotnik A.
J. Neuroimmunol. 171:145-155(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Structure analysis of human class-I MHC restricted T-cell associated molecule."
New York structural genomics research consortium (NYSGRC)
Submitted (MAY-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 18-117, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001622 mRNA. Translation: AAC80267.1.
AB209830 mRNA. Translation: BAD93067.1. Different initiation.
BC070266 mRNA. Translation: AAH70266.1.
RefSeqNP_062550.2. NM_019604.2.
UniGeneHs.159523.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RBGX-ray2.30A/B/C/D18-117[»]
4H5SX-ray1.70A18-117[»]
ProteinModelPortalO95727.
SMRO95727. Positions 16-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60155N.
STRING9606.ENSP00000227348.

Proteomic databases

PaxDbO95727.
PRIDEO95727.

Protocols and materials databases

DNASU56253.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000227348; ENSP00000227348; ENSG00000109943. [O95727-1]
ENST00000533709; ENSP00000433728; ENSG00000109943. [O95727-2]
GeneID56253.
KEGGhsa:56253.
UCSCuc001pyj.3. human. [O95727-1]
uc001pyk.3. human. [O95727-2]

Organism-specific databases

CTD56253.
GeneCardsGC11P122743.
H-InvDBHIX0035844.
HGNCHGNC:24313. CRTAM.
HPAHPA054595.
MIM612597. gene.
neXtProtNX_O95727.
PharmGKBPA145149072.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72056.
HOGENOMHOG000008690.
HOVERGENHBG053014.
InParanoidO95727.
KOK16361.
OMAHVIWKKE.
OrthoDBEOG7PVWPD.
PhylomeDBO95727.
TreeFamTF326804.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

BgeeO95727.
CleanExHS_CRTAM.
GenevestigatorO95727.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95727.
GenomeRNAi56253.
NextBio61889.
PROO95727.
SOURCESearch...

Entry information

Entry nameCRTAM_HUMAN
AccessionPrimary (citable) accession number: O95727
Secondary accession number(s): Q59EI1, Q6IRX2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries