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O95727

- CRTAM_HUMAN

UniProt

O95727 - CRTAM_HUMAN

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Protein

Cytotoxic and regulatory T-cell molecule

Gene

CRTAM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.By similarity1 Publication

GO - Molecular functioni

  1. receptor binding Source: UniProtKB

GO - Biological processi

  1. cell recognition Source: HGNC
  2. detection of stimulus Source: HGNC
  3. detection of tumor cell Source: HGNC
  4. immune system process Source: UniProtKB-KW
  5. positive regulation of cytokine secretion Source: HGNC
  6. positive regulation of natural killer cell mediated cytotoxicity Source: HGNC
  7. positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: HGNC
  8. regulation of immune response Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxic and regulatory T-cell molecule
Alternative name(s):
Class-I MHC-restricted T-cell-associated molecule
CD_antigen: CD355
Gene namesi
Name:CRTAMImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:24313. CRTAM.

Subcellular locationi

Membrane Sequence Analysis; Single-pass type I membrane protein Sequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145149072.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 393376Cytotoxic and regulatory T-cell moleculePRO_0000292602Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi38 ↔ 981 PublicationPROSITE-ProRule annotation
Glycosylationi87 – 871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi141 ↔ 196PROSITE-ProRule annotation
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO95727.
PRIDEiO95727.

Expressioni

Tissue specificityi

In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues.3 Publications

Gene expression databases

BgeeiO95727.
CleanExiHS_CRTAM.
GenevestigatoriO95727.

Organism-specific databases

HPAiHPA054595.

Interactioni

Subunit structurei

Interacts with CADM1.2 Publications

Protein-protein interaction databases

DIPiDIP-60155N.
STRINGi9606.ENSP00000227348.

Structurei

Secondary structure

1
393
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 296
Beta strandi34 – 396
Beta strandi47 – 515
Beta strandi57 – 604
Beta strandi72 – 776
Beta strandi80 – 856
Helixi90 – 923
Beta strandi94 – 11522

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3RBGX-ray2.30A/B/C/D18-117[»]
4H5SX-ray1.70A18-117[»]
ProteinModelPortaliO95727.
SMRiO95727. Positions 22-185.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95727.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 287270ExtracellularSequence AnalysisAdd
BLAST
Topological domaini309 – 39385CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 11497Ig-like V-typeSequence AnalysisAdd
BLAST
Domaini118 – 21093Ig-like C2-typeSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the nectin family.1 Publication
Contains 1 Ig-like V-type (immunoglobulin-like) domain.Sequence Analysis

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72056.
GeneTreeiENSGT00760000119054.
HOGENOMiHOG000008690.
HOVERGENiHBG053014.
InParanoidiO95727.
KOiK16361.
OMAiHVIWKKE.
OrthoDBiEOG7PVWPD.
PhylomeDBiO95727.
TreeFamiTF326804.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: O95727-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW
60 70 80 90 100
LTPSGFTIFL NEYPALKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH
110 120 130 140 150
YSDSVSTKEV KVIVLATPFK PILEASVIRK QNGEEHVVLM CSTMRSKPPP
160 170 180 190 200
QITWLLGNSM EVSGGTLHEF ETDGKKCNTT STLIIHTYGK NSTVDCIIRH
210 220 230 240 250
RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ PTSTVSVTED
260 270 280 290 300
SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI
310 320 330 340 350
LFIIVQLFIM KLRKAHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ
360 370 380 390
SSHPMRCMNY ITKLYSEAKT KRKENVQHSK LEEKHIQVPE SIV
Length:393
Mass (Da):44,641
Last modified:June 26, 2007 - v2
Checksum:iCB8173032EE45F03
GO
Isoform 2 (identifier: O95727-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     200-216: HRGLQGRKLVAPFRFED → MWVKLLSIVAEFCFSPF

Note: No experimental confirmation available.Curated

Show »
Length:194
Mass (Da):22,256
Checksum:i2CD6A68172F93C8B
GO

Sequence cautioni

The sequence BAD93067.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → V in AAH70266. (PubMed:15489334)Curated
Sequence conflicti315 – 3151A → T in AAH70266. (PubMed:15489334)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti16 – 161E → A.
Corresponds to variant rs35411582 [ dbSNP | Ensembl ].
VAR_049868
Natural varianti78 – 781A → D.
Corresponds to variant rs34397316 [ dbSNP | Ensembl ].
VAR_049869
Natural varianti173 – 1731D → G.
Corresponds to variant rs35136295 [ dbSNP | Ensembl ].
VAR_049870
Natural varianti321 – 3211K → R.1 Publication
Corresponds to variant rs2272094 [ dbSNP | Ensembl ].
VAR_032999
Natural varianti368 – 3681A → G.1 Publication
Corresponds to variant rs1916036 [ dbSNP | Ensembl ].
VAR_033000

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 199199Missing in isoform 2. 1 PublicationVSP_052471Add
BLAST
Alternative sequencei200 – 21617HRGLQ…FRFED → MWVKLLSIVAEFCFSPF in isoform 2. 1 PublicationVSP_052472Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001622 mRNA. Translation: AAC80267.1.
AB209830 mRNA. Translation: BAD93067.1. Different initiation.
BC070266 mRNA. Translation: AAH70266.1.
CCDSiCCDS8437.1. [O95727-1]
RefSeqiNP_062550.2. NM_019604.2. [O95727-1]
UniGeneiHs.159523.

Genome annotation databases

EnsembliENST00000227348; ENSP00000227348; ENSG00000109943. [O95727-1]
ENST00000533709; ENSP00000433728; ENSG00000109943. [O95727-2]
GeneIDi56253.
KEGGihsa:56253.
UCSCiuc001pyj.3. human. [O95727-1]
uc001pyk.3. human. [O95727-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF001622 mRNA. Translation: AAC80267.1 .
AB209830 mRNA. Translation: BAD93067.1 . Different initiation.
BC070266 mRNA. Translation: AAH70266.1 .
CCDSi CCDS8437.1. [O95727-1 ]
RefSeqi NP_062550.2. NM_019604.2. [O95727-1 ]
UniGenei Hs.159523.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3RBG X-ray 2.30 A/B/C/D 18-117 [» ]
4H5S X-ray 1.70 A 18-117 [» ]
ProteinModelPortali O95727.
SMRi O95727. Positions 22-185.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60155N.
STRINGi 9606.ENSP00000227348.

Proteomic databases

PaxDbi O95727.
PRIDEi O95727.

Protocols and materials databases

DNASUi 56253.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000227348 ; ENSP00000227348 ; ENSG00000109943 . [O95727-1 ]
ENST00000533709 ; ENSP00000433728 ; ENSG00000109943 . [O95727-2 ]
GeneIDi 56253.
KEGGi hsa:56253.
UCSCi uc001pyj.3. human. [O95727-1 ]
uc001pyk.3. human. [O95727-2 ]

Organism-specific databases

CTDi 56253.
GeneCardsi GC11P122743.
H-InvDB HIX0035844.
HGNCi HGNC:24313. CRTAM.
HPAi HPA054595.
MIMi 612597. gene.
neXtProti NX_O95727.
PharmGKBi PA145149072.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG72056.
GeneTreei ENSGT00760000119054.
HOGENOMi HOG000008690.
HOVERGENi HBG053014.
InParanoidi O95727.
KOi K16361.
OMAi HVIWKKE.
OrthoDBi EOG7PVWPD.
PhylomeDBi O95727.
TreeFami TF326804.

Enzyme and pathway databases

Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTracei O95727.
GenomeRNAii 56253.
NextBioi 61889.
PROi O95727.
SOURCEi Search...

Gene expression databases

Bgeei O95727.
CleanExi HS_CRTAM.
Genevestigatori O95727.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM)."
    Kennedy J., Vicari A.P., Saylor V., Zurawski S.M., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A.
    J. Leukoc. Biol. 67:725-734(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT ARG-321.
  2. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: BrainImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-368.
    Tissue: Peripheral bloodImported.
  4. "The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM."
    Boles K.S., Barchet W., Diacovo T., Cella M., Colonna M.
    Blood 106:779-786(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CADM1, TISSUE SPECIFICITY.
  5. "Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule."
    Galibert L., Diemer G.S., Liu Z., Johnson R.S., Smith J.L., Walzer T., Comeau M.R., Rauch C.T., Wolfson M.F., Sorensen R.A., Van der Vuurst de Vries A.-R., Branstetter D.G., Koelling R.M., Scholler J., Fanslow W.C., Baum P.R., Derry J.M., Yan W.
    J. Biol. Chem. 280:21955-21964(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CADM1.
  6. "Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes."
    Patino-Lopez G., Hevezi P., Lee J., Willhite D., Verge G.M., Lechner S.M., Ortiz-Navarrete V., Zlotnik A.
    J. Neuroimmunol. 171:145-155(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Structure analysis of human class-I MHC restricted T-cell associated molecule."
    New York structural genomics research consortium (NYSGRC)
    Submitted (MAY-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 18-117, DISULFIDE BOND.

Entry informationi

Entry nameiCRTAM_HUMAN
AccessioniPrimary (citable) accession number: O95727
Secondary accession number(s): Q59EI1, Q6IRX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3