O95727 (CRTAM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytotoxic and regulatory T-cell molecule

Alternative name(s):
Class-I MHC-restricted T-cell-associated molecule
CD_antigen=CD355

Gene names

Name:

CRTAM

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Ref.4 UniProtKB Q149L7
Subunit structure	Interacts with CADM1. Ref.4 Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. Ref.1 Ref.4 Ref.6
Sequence similarities	Belongs to the nectin family. Ref.6 Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Sequence caution	The sequence BAD93067.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell recognition Inferred from direct assay Ref.4. Source: HGNC detection of tumor cell Inferred from direct assay Ref.4. Source: HGNC positive regulation of cytokine secretion Inferred from direct assay Ref.4. Source: HGNC positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Inferred from direct assay Ref.4. Source: HGNC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.4. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: O95727-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: O95727-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     200-216: HRGLQGRKLVAPFRFED → MWVKLLSIVAEFCFSPF

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Chain

18 – 393

376

Cytotoxic and regulatory T-cell molecule

PRO_0000292602

Regions

Topological domain

18 – 287

270

Extracellular Potential

Transmembrane

288 – 308

21

Helical; Potential

Topological domain

309 – 393

85

Cytoplasmic Potential

Domain

18 – 114

97

Ig-like V-type

Domain

118 – 210

93

Ig-like C2-type

Amino acid modifications

Glycosylation

21

1

N-linked (GlcNAc...) Potential

Glycosylation

87

1

N-linked (GlcNAc...) Potential

Glycosylation

178

1

N-linked (GlcNAc...) Potential

Disulfide bond

38 ↔ 98

Ref.7

Disulfide bond

141 ↔ 196

By similarity

Natural variations

Alternative sequence

1 – 199

199

Missing in isoform 2. Ref.2

VSP_052471

Alternative sequence

200 – 216

17

HRGLQ…FRFED → MWVKLLSIVAEFCFSPF in isoform 2. Ref.2

VSP_052472

Natural variant

16

1

E → A.
Corresponds to variant rs35411582 [ dbSNP | Ensembl ].

VAR_049868

Natural variant

78

1

A → D.
Corresponds to variant rs34397316 [ dbSNP | Ensembl ].

VAR_049869

Natural variant

173

1

D → G.
Corresponds to variant rs35136295 [ dbSNP | Ensembl ].

VAR_049870

Natural variant

321

1

K → R. Ref.1
Corresponds to variant rs2272094 [ dbSNP | Ensembl ].

VAR_032999

Natural variant

368

1

A → G. Ref.3
Corresponds to variant rs1916036 [ dbSNP | Ensembl ].

VAR_033000

Experimental info

Sequence conflict

65

1

A → V in AAH70266. Ref.3

Sequence conflict

315

1

A → T in AAH70266. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

393

Helix Strand Turn

Details...

Beta strand

22 – 29

8

Beta strand

34 – 39

6

Beta strand

47 – 51

5

Beta strand

57 – 60

4

Beta strand

72 – 77

6

Beta strand

80 – 87

8

Helix

90 – 92

3

Beta strand

94 – 115

22

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: CB8173032EE45F03	FASTA	393	44,641
	10 20 30 40 50 60 MWWRVLSLLA WFPLQEASLT NHTETITVEE GQTLTLKCVT SLRKNSSLQW LTPSGFTIFL 70 80 90 100 110 120 NEYPALKNSK YQLLHHSANQ LSITVPNVTL QDEGVYKCLH YSDSVSTKEV KVIVLATPFK 130 140 150 160 170 180 PILEASVIRK QNGEEHVVLM CSTMRSKPPP QITWLLGNSM EVSGGTLHEF ETDGKKCNTT 190 200 210 220 230 240 STLIIHTYGK NSTVDCIIRH RGLQGRKLVA PFRFEDLVTD EETASDALER NSLSSQDPQQ 250 260 270 280 290 300 PTSTVSVTED SSTSEIDKEE KEQTTQDPDL TTEANPQYLG LARKKSGILL LTLVSFLIFI 310 320 330 340 350 360 LFIIVQLFIM KLRKAHVIWK KENEVSEHTL ESYRSRSNNE ETSSEEKNGQ SSHPMRCMNY 370 380 390 ITKLYSEAKT KRKENVQHSK LEEKHIQVPE SIV
	Isoform 2 [UniParc]. Checksum: 2CD6A68172F93C8B	FASTA	194	22,256
	10 20 30 40 50 60 MWVKLLSIVA EFCFSPFLVT DEETASDALE RNSLSSQDPQ QPTSTVSVTE DSSTSEIDKE 70 80 90 100 110 120 EKEQTTQDPD LTTEANPQYL GLARKKSGIL LLTLVSFLIF ILFIIVQLFI MKLRKAHVIW 130 140 150 160 170 180 KKENEVSEHT LESYRSRSNN EETSSEEKNG QSSHPMRCMN YITKLYSEAK TKRKENVQHS 190 KLEEKHIQVP ESIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM)." Kennedy J., Vicari A.P., Saylor V., Zurawski S.M., Copeland N.G., Gilbert D.J., Jenkins N.A., Zlotnik A. J. Leukoc. Biol. 67:725-734(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT ARG-321.
[2]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-368. Tissue: Peripheral blood.
[4]	"The tumor suppressor TSLC1/NECL-2 triggers NK-cell and CD8+ T-cell responses through the cell-surface receptor CRTAM." Boles K.S., Barchet W., Diacovo T., Cella M., Colonna M. Blood 106:779-786(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CADM1, TISSUE SPECIFICITY.
[5]	"Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a ligand for class-I-restricted T-cell-associated molecule." Galibert L., Diemer G.S., Liu Z., Johnson R.S., Smith J.L., Walzer T., Comeau M.R., Rauch C.T., Wolfson M.F., Sorensen R.A., Van der Vuurst de Vries A.-R., Branstetter D.G., Koelling R.M., Scholler J., Fanslow W.C., Baum P.R., Derry J.M., Yan W. J. Biol. Chem. 280:21955-21964(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CADM1.
[6]	"Human class-I restricted T cell associated molecule is highly expressed in the cerebellum and is a marker for activated NKT and CD8+ T lymphocytes." Patino-Lopez G., Hevezi P., Lee J., Willhite D., Verge G.M., Lechner S.M., Ortiz-Navarrete V., Zlotnik A. J. Neuroimmunol. 171:145-155(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Structure analysis of human class-I MHC restricted T-cell associated molecule." New York structural genomics research consortium (NYSGRC) Submitted (MAY-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 18-117, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF001622 mRNA. Translation: AAC80267.1.
AB209830 mRNA. Translation: BAD93067.1. Different initiation.
BC070266 mRNA. Translation: AAH70266.1.

IPI

IPI00159048.
IPI00847808.

RefSeq

NP_062550.2. NM_019604.2.

UniGene

Hs.159523.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RBG	X-ray	2.30	A/B/C/D	18-117	[»]

ProteinModelPortal

O95727.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000227348.

Proteomic databases

PaxDb

O95727.

PRIDE

O95727.

Protocols and materials databases

DNASU

56253.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000227348; ENSP00000227348; ENSG00000109943.
ENST00000533709; ENSP00000433728; ENSG00000109943.

GeneID

56253.

KEGG

hsa:56253.

UCSC

uc001pyj.3. human.
uc001pyk.3. human.

Organism-specific databases

CTD

56253.

GeneCards

GC11P122743.

H-InvDB

HIX0035844.

HGNC

HGNC:24313. CRTAM.

HPA

HPA054595.

MIM

612597. gene.

neXtProt

NX_O95727.

PharmGKB

PA145149072.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG72056.

HOGENOM

HOG000008690.

HOVERGEN

HBG053014.

InParanoid

O95727.

KO

K16361.

OMA

KKCNTTS.

OrthoDB

EOG4ZKJMT.

PhylomeDB

O95727.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

Bgee

O95727.

CleanEx

HS_CRTAM.

Genevestigator

O95727.

Family and domain databases

Gene3D

2.60.40.10. 2 hits.

InterPro

IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]

Pfam

PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O95727.

GenomeRNAi

56253.

NextBio

61889.

SOURCE

Search...

Entry information

Entry name

CRTAM_HUMAN

Accession

Primary (citable) accession number: O95727
Secondary accession number(s): Q59EI1, Q6IRX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.