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O95715 (CXL14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C-X-C motif chemokine 14
Alternative name(s):
Chemokine BRAK
MIP-2G
Small-inducible cytokine B14
Gene names
Name:CXCL14
Synonyms:MIP2G, NJAC, SCYB14
ORF Names:PSEC0212, UNQ240/PRO273
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length111 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Ref.2 Ref.8

Subcellular location

Secreted.

Tissue specificity

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. Ref.1 Ref.2 Ref.8

Induction

Up-regulated in peripheral blood lymphocytes in response to bacterial lipopolysaccharides (LPS). Ref.1

Domain

The destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway. Ref.9

Post-translational modification

Ubiquitinated, followed by degradation by the proteasome. Ref.9

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Caution

It is uncertain whether Met-1 or Met-13 is the initiator.

Sequence caution

The sequence AAD38944.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434 Ref.2
Chain35 – 11177C-X-C motif chemokine 14
PRO_0000005115

Regions

Motif67 – 8115D-box

Amino acid modifications

Disulfide bond37 ↔ 63 Ref.9
Disulfide bond39 ↔ 84 Ref.9

Experimental info

Sequence conflict1081V → F in AAF78449. Ref.2

Secondary structure

................ 111
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O95715 [UniParc].

Last modified March 23, 2010. Version 2.
Checksum: C9A18B2A78CACF74

FASTA11113,078
        10         20         30         40         50         60 
MSLLPRRAPP VSMRLLAAAL LLLLLALYTA RVDGSKCKCS RKGPKIRYSD VKKLEMKPKY 

        70         80         90        100        110 
PHCEEKMVII TTKSVSRYRG QEHCLHPKLQ STKRFIKWYN AWNEKRRVYE E 

« Hide

References

« Hide 'large scale' references
[1]"In vivo expression of the novel CXC chemokine BRAK in normal and cancerous human tissue."
Frederick M.J., Henderson Y., Xu X., Deavers M.T., Sahin A.A., Wu H., Lewis D.E., El-Naggar A.K., Clayman G.L.
Am. J. Pathol. 156:1937-1950(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Tissue: Oral epithelium.
[2]"Molecular cloning and characterization of a novel CXC chemokine macrophage inflammatory protein-2 gamma chemoattractant for human neutrophils and dendritic cells."
Cao X., Zhang W., Wan T., He L., Chen T., Yuan Z., Ma S., Yu Y., Chen G.
J. Immunol. 165:2588-2595(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-44, FUNCTION, TISSUE SPECIFICITY.
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
[5]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[8]"Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells."
Hromas R., Broxmeyer H.E., Kim C., Nakshatri H., Christopherson K. II, Azam M., Hou Y.-H.
Biochem. Biophys. Res. Commun. 255:703-706(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-111, FUNCTION, TISSUE SPECIFICITY.
[9]"Structural determinants involved in the regulation of CXCL14/BRAK expression by the 26 S proteasome."
Peterson F.C., Thorpe J.A., Harder A.G., Volkman B.F., Schwarze S.R.
J. Mol. Biol. 363:813-822(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 34-111, DOMAIN D-BOX MOTIF, UBIQUITINATION, DISULFIDE BONDS.
+Additional computationally mapped references.

Web resources

Wikipedia

CXCL14 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF144103 mRNA. Translation: AAD38944.1. Different initiation.
AF106911 mRNA. Translation: AAF78449.1.
AY358906 mRNA. Translation: AAQ89265.1.
AK075514 mRNA. Translation: BAG52160.1.
BT007080 mRNA. Translation: AAP35743.1.
AC034206 Genomic DNA. No translation available.
BC003513 mRNA. Translation: AAH03513.1.
AF073957 mRNA. Translation: AAD03839.1.
PIRJG0182.
RefSeqNP_004878.2. NM_004887.4.
UniGeneHs.483444.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HDLNMR-A34-111[»]
ProteinModelPortalO95715.
SMRO95715. Positions 35-111.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114921. 2 interactions.
IntActO95715. 4 interactions.
MINTMINT-4724169.
STRING9606.ENSP00000337065.

PTM databases

PhosphoSiteO95715.

Proteomic databases

PaxDbO95715.
PRIDEO95715.

Protocols and materials databases

DNASU9547.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000337225; ENSP00000337065; ENSG00000145824.
ENST00000512158; ENSP00000423783; ENSG00000145824.
GeneID9547.
KEGGhsa:9547.
UCSCuc003lay.3. human.

Organism-specific databases

CTD9547.
GeneCardsGC05M134934.
HGNCHGNC:10640. CXCL14.
HPACAB029996.
MIM604186. gene.
neXtProtNX_O95715.
PharmGKBPA35571.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG72608.
HOGENOMHOG000065700.
HOVERGENHBG054250.
InParanoidO95715.
KOK10033.
OMAEGSKCKC.
OrthoDBEOG7TXKK6.
PhylomeDBO95715.
TreeFamTF332769.

Gene expression databases

BgeeO95715.
CleanExHS_CXCL14.
GenevestigatorO95715.

Family and domain databases

InterProIPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PfamPF00048. IL8. 1 hit.
[Graphical view]
SUPFAMSSF54117. SSF54117. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCXCL14. human.
EvolutionaryTraceO95715.
GenomeRNAi9547.
NextBio35803.
PROO95715.
SOURCESearch...

Entry information

Entry nameCXL14_HUMAN
AccessionPrimary (citable) accession number: O95715
Secondary accession number(s): B3KQU8 expand/collapse secondary AC list , Q6UW97, Q86U69, Q9BTR1, Q9NS21
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM