O95715 (CXL14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-X-C motif chemokine 14

Alternative name(s):
Chemokine BRAK
MIP-2G
Small-inducible cytokine B14

Gene names

Name:	CXCL14
Synonyms:	MIP2G, NJAC, SCYB14
ORF Names:	PSEC0212, UNQ240/PRO273

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Ref.2 Ref.8
Subcellular location	Secreted.
Tissue specificity	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. Ref.1 Ref.2 Ref.8
Induction	Up-regulated in peripheral blood lymphocytes in response to bacterial lipopolysaccharides (LPS). Ref.1
Domain	The destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
Post-translational modification	Ubiquitinated, followed by degradation by the proteasome. Ref.9
Sequence similarities	Belongs to the intercrine alpha (chemokine CxC) family.
Caution	It is uncertain whether Met-1 or Met-13 is the initiator.
Sequence caution	The sequence AAD38944.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Ubl conjugation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.8. Source: ProtInc chemotaxis Traceable author statement Ref.8. Source: ProtInc immune response Inferred from electronic annotation. Source: InterPro inner ear development Inferred from electronic annotation. Source: Compara signal transduction Traceable author statement Ref.8. Source: ProtInc
Cellular_component	Golgi apparatus Inferred from direct assay. Source: LIFEdb extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Traceable author statement Ref.8. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

34

Ref.2

Chain

35 – 111

77

C-X-C motif chemokine 14

PRO_0000005115

Regions

Motif

67 – 81

15

D-box

Amino acid modifications

Disulfide bond

37 ↔ 63

Ref.9

Disulfide bond

39 ↔ 84

Ref.9

Experimental info

Sequence conflict

108

1

V → F in AAF78449. Ref.2

Secondary structure

1

.

.

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.

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.

.

111

Helix Strand Turn

Details...

Beta strand

41 – 44

4

Helix

48 – 50

3

Beta strand

51 – 56

6

Beta strand

63 – 65

3

Beta strand

68 – 72

5

Beta strand

74 – 76

3

Turn

77 – 80

4

Beta strand

81 – 85

5

Helix

90 – 106

17

Sequences

	Sequence		Length	Mass (Da)
	O95715 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: C9A18B2A78CACF74	FASTA	111	13,078
	10 20 30 40 50 60 MSLLPRRAPP VSMRLLAAAL LLLLLALYTA RVDGSKCKCS RKGPKIRYSD VKKLEMKPKY 70 80 90 100 110 PHCEEKMVII TTKSVSRYRG QEHCLHPKLQ STKRFIKWYN AWNEKRRVYE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In vivo expression of the novel CXC chemokine BRAK in normal and cancerous human tissue." Frederick M.J., Henderson Y., Xu X., Deavers M.T., Sahin A.A., Wu H., Lewis D.E., El-Naggar A.K., Clayman G.L. Am. J. Pathol. 156:1937-1950(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Tissue: Oral epithelium.
[2]	"Molecular cloning and characterization of a novel CXC chemokine macrophage inflammatory protein-2 gamma chemoattractant for human neutrophils and dendritic cells." Cao X., Zhang W., Wan T., He L., Chen T., Yuan Z., Ma S., Yu Y., Chen G. J. Immunol. 165:2588-2595(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-44, FUNCTION, TISSUE SPECIFICITY.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[8]	"Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells." Hromas R., Broxmeyer H.E., Kim C., Nakshatri H., Christopherson K. II, Azam M., Hou Y.-H. Biochem. Biophys. Res. Commun. 255:703-706(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-111, FUNCTION, TISSUE SPECIFICITY.
[9]	"Structural determinants involved in the regulation of CXCL14/BRAK expression by the 26 S proteasome." Peterson F.C., Thorpe J.A., Harder A.G., Volkman B.F., Schwarze S.R. J. Mol. Biol. 363:813-822(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 34-111, MOTIF D-BOX, UBIQUITINATION, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Wikipedia

CXCL14 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF144103 mRNA. Translation: AAD38944.1. Different initiation.
AF106911 mRNA. Translation: AAF78449.1.
AY358906 mRNA. Translation: AAQ89265.1.
AK075514 mRNA. Translation: BAG52160.1.
BT007080 mRNA. Translation: AAP35743.1.
AC034206 Genomic DNA. No translation available.
BC003513 mRNA. Translation: AAH03513.1.
AF073957 mRNA. Translation: AAD03839.1.

IPI

IPI00396257.

PIR

JG0182.

RefSeq

NP_004878.2. NM_004887.4.

UniGene

Hs.483444.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HDL	NMR	-	A	34-111	[»]

ProteinModelPortal

O95715.

SMR

O95715. Positions 35-111.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95715. 3 interactions.

MINT

MINT-4724169.

STRING

9606.ENSP00000337065.

PTM databases

PhosphoSite

O95715.

Proteomic databases

PaxDb

O95715.

PRIDE

O95715.

Protocols and materials databases

DNASU

9547.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000337225; ENSP00000337065; ENSG00000145824.
ENST00000512158; ENSP00000423783; ENSG00000145824.

GeneID

9547.

KEGG

hsa:9547.

UCSC

uc003lay.3. human.

Organism-specific databases

CTD

9547.

GeneCards

GC05M134934.

HGNC

HGNC:10640. CXCL14.

HPA

CAB029996.

MIM

604186. gene.

neXtProt

NX_O95715.

PharmGKB

PA35571.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG72608.

HOGENOM

HOG000065700.

HOVERGEN

HBG054250.

InParanoid

O95715.

KO

K10033.

OMA

EGSKCKC.

OrthoDB

EOG4H72D5.

PhylomeDB

O95715.

Gene expression databases

Bgee

O95715.

CleanEx

HS_CXCL14.

Genevestigator

O95715.

GermOnline

ENSG00000145824. Homo sapiens.

Family and domain databases

InterPro

IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00471. SMALL_CYTOKINES_CXC. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CXCL14. human.

EvolutionaryTrace

O95715.

GenomeRNAi

9547.

NextBio

35803.

SOURCE

Search...

Entry information

Entry name

CXL14_HUMAN

Accession

Primary (citable) accession number: O95715
Secondary accession number(s): B3KQU8 , Q6UW97, Q86U69, Q9BTR1, Q9NS21

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 23, 2010
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.