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O95715

- CXL14_HUMAN

UniProt

O95715 - CXL14_HUMAN

Protein

C-X-C motif chemokine 14

Gene

CXCL14

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.2 Publications

    GO - Molecular functioni

    1. chemokine activity Source: ProtInc

    GO - Biological processi

    1. cell-cell signaling Source: ProtInc
    2. cell chemotaxis Source: GOC
    3. chemotaxis Source: ProtInc
    4. immune response Source: InterPro
    5. inner ear development Source: Ensembl
    6. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Cytokine

    Keywords - Biological processi

    Chemotaxis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-X-C motif chemokine 14
    Alternative name(s):
    Chemokine BRAK
    MIP-2G
    Small-inducible cytokine B14
    Gene namesi
    Name:CXCL14
    Synonyms:MIP2G, NJAC, SCYB14
    ORF Names:PSEC0212, UNQ240/PRO273
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:10640. CXCL14.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-KW
    2. Golgi apparatus Source: LIFEdb

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35571.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 34341 PublicationAdd
    BLAST
    Chaini35 – 11177C-X-C motif chemokine 14PRO_0000005115Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi37 ↔ 631 Publication
    Disulfide bondi39 ↔ 841 Publication

    Post-translational modificationi

    Ubiquitinated, followed by degradation by the proteasome.1 Publication

    Keywords - PTMi

    Disulfide bond, Ubl conjugation

    Proteomic databases

    PaxDbiO95715.
    PRIDEiO95715.

    PTM databases

    PhosphoSiteiO95715.

    Expressioni

    Tissue specificityi

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.3 Publications

    Inductioni

    Up-regulated in peripheral blood lymphocytes in response to bacterial lipopolysaccharides (LPS).1 Publication

    Gene expression databases

    BgeeiO95715.
    CleanExiHS_CXCL14.
    GenevestigatoriO95715.

    Organism-specific databases

    HPAiCAB029996.

    Interactioni

    Protein-protein interaction databases

    BioGridi114921. 2 interactions.
    IntActiO95715. 4 interactions.
    MINTiMINT-4724169.
    STRINGi9606.ENSP00000337065.

    Structurei

    Secondary structure

    1
    111
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi41 – 444
    Helixi48 – 503
    Beta strandi51 – 566
    Beta strandi63 – 653
    Beta strandi68 – 725
    Beta strandi74 – 763
    Turni77 – 804
    Beta strandi81 – 855
    Helixi90 – 10617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HDLNMR-A35-111[»]
    ProteinModelPortaliO95715.
    SMRiO95715. Positions 35-111.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95715.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi67 – 8115D-boxAdd
    BLAST

    Domaini

    The destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG72608.
    HOGENOMiHOG000065700.
    HOVERGENiHBG054250.
    InParanoidiO95715.
    KOiK10033.
    OMAiMCLASAE.
    OrthoDBiEOG7TXKK6.
    PhylomeDBiO95715.
    TreeFamiTF332769.

    Family and domain databases

    InterProiIPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O95715-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLLPRRAPP VSMRLLAAAL LLLLLALYTA RVDGSKCKCS RKGPKIRYSD    50
    VKKLEMKPKY PHCEEKMVII TTKSVSRYRG QEHCLHPKLQ STKRFIKWYN 100
    AWNEKRRVYE E 111
    Length:111
    Mass (Da):13,078
    Last modified:March 23, 2010 - v2
    Checksum:iC9A18B2A78CACF74
    GO

    Sequence cautioni

    The sequence AAD38944.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081V → F in AAF78449. (PubMed:10946286)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF144103 mRNA. Translation: AAD38944.1. Different initiation.
    AF106911 mRNA. Translation: AAF78449.1.
    AY358906 mRNA. Translation: AAQ89265.1.
    AK075514 mRNA. Translation: BAG52160.1.
    BT007080 mRNA. Translation: AAP35743.1.
    AC034206 Genomic DNA. No translation available.
    BC003513 mRNA. Translation: AAH03513.1.
    AF073957 mRNA. Translation: AAD03839.1.
    CCDSiCCDS4188.1.
    PIRiJG0182.
    RefSeqiNP_004878.2. NM_004887.4.
    UniGeneiHs.483444.

    Genome annotation databases

    EnsembliENST00000337225; ENSP00000337065; ENSG00000145824.
    ENST00000512158; ENSP00000423783; ENSG00000145824.
    GeneIDi9547.
    KEGGihsa:9547.
    UCSCiuc003lay.3. human.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CXCL14 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF144103 mRNA. Translation: AAD38944.1 . Different initiation.
    AF106911 mRNA. Translation: AAF78449.1 .
    AY358906 mRNA. Translation: AAQ89265.1 .
    AK075514 mRNA. Translation: BAG52160.1 .
    BT007080 mRNA. Translation: AAP35743.1 .
    AC034206 Genomic DNA. No translation available.
    BC003513 mRNA. Translation: AAH03513.1 .
    AF073957 mRNA. Translation: AAD03839.1 .
    CCDSi CCDS4188.1.
    PIRi JG0182.
    RefSeqi NP_004878.2. NM_004887.4.
    UniGenei Hs.483444.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HDL NMR - A 35-111 [» ]
    ProteinModelPortali O95715.
    SMRi O95715. Positions 35-111.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114921. 2 interactions.
    IntActi O95715. 4 interactions.
    MINTi MINT-4724169.
    STRINGi 9606.ENSP00000337065.

    PTM databases

    PhosphoSitei O95715.

    Proteomic databases

    PaxDbi O95715.
    PRIDEi O95715.

    Protocols and materials databases

    DNASUi 9547.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000337225 ; ENSP00000337065 ; ENSG00000145824 .
    ENST00000512158 ; ENSP00000423783 ; ENSG00000145824 .
    GeneIDi 9547.
    KEGGi hsa:9547.
    UCSCi uc003lay.3. human.

    Organism-specific databases

    CTDi 9547.
    GeneCardsi GC05M134934.
    HGNCi HGNC:10640. CXCL14.
    HPAi CAB029996.
    MIMi 604186. gene.
    neXtProti NX_O95715.
    PharmGKBi PA35571.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG72608.
    HOGENOMi HOG000065700.
    HOVERGENi HBG054250.
    InParanoidi O95715.
    KOi K10033.
    OMAi MCLASAE.
    OrthoDBi EOG7TXKK6.
    PhylomeDBi O95715.
    TreeFami TF332769.

    Miscellaneous databases

    ChiTaRSi CXCL14. human.
    EvolutionaryTracei O95715.
    GenomeRNAii 9547.
    NextBioi 35803.
    PROi O95715.
    SOURCEi Search...

    Gene expression databases

    Bgeei O95715.
    CleanExi HS_CXCL14.
    Genevestigatori O95715.

    Family and domain databases

    InterProi IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "In vivo expression of the novel CXC chemokine BRAK in normal and cancerous human tissue."
      Frederick M.J., Henderson Y., Xu X., Deavers M.T., Sahin A.A., Wu H., Lewis D.E., El-Naggar A.K., Clayman G.L.
      Am. J. Pathol. 156:1937-1950(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Tissue: Oral epithelium.
    2. "Molecular cloning and characterization of a novel CXC chemokine macrophage inflammatory protein-2 gamma chemoattractant for human neutrophils and dendritic cells."
      Cao X., Zhang W., Wan T., He L., Chen T., Yuan Z., Ma S., Yu Y., Chen G.
      J. Immunol. 165:2588-2595(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-44, FUNCTION, TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.
    5. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    8. "Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells."
      Hromas R., Broxmeyer H.E., Kim C., Nakshatri H., Christopherson K. II, Azam M., Hou Y.-H.
      Biochem. Biophys. Res. Commun. 255:703-706(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-111, FUNCTION, TISSUE SPECIFICITY.
    9. "Structural determinants involved in the regulation of CXCL14/BRAK expression by the 26 S proteasome."
      Peterson F.C., Thorpe J.A., Harder A.G., Volkman B.F., Schwarze S.R.
      J. Mol. Biol. 363:813-822(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 34-111, DOMAIN D-BOX MOTIF, UBIQUITINATION, DISULFIDE BONDS.

    Entry informationi

    Entry nameiCXL14_HUMAN
    AccessioniPrimary (citable) accession number: O95715
    Secondary accession number(s): B3KQU8
    , Q6UW97, Q86U69, Q9BTR1, Q9NS21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-13 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3