O95711 (LY86_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphocyte antigen 86
Short name=Ly-86

Alternative name(s):
Protein MD-1

Gene names

Name:	LY86
Synonyms:	MD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression By similarity.
Subunit structure	M-shaped tetramer of two CD180-LY86 heterodimers. Ref.6
Subcellular location	Secreted › extracellular space. Note: Associated with CD180 at the cell surface.
Tissue specificity	Highly expressed in B-cells, monocytes and tonsil.
Induction	In monocytes, down-regulated by the cell-wall fraction of Mycobacterium bovis (BCG-CWS).
Sequence caution	The sequence BAA76410.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement Ref.1. Source: ProtInc cell proliferation Traceable author statement Ref.1. Source: ProtInc humoral immune response Traceable author statement Ref.1. Source: ProtInc inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of lipopolysaccharide-mediated signaling pathway Inferred from genetic interaction PubMed 10880523. Source: MGI
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Ref.5

Chain

21 – 162

142

Lymphocyte antigen 86

PRO_0000018614

Amino acid modifications

Glycosylation

96

1

N-linked (GlcNAc...) Potential

Glycosylation

156

1

N-linked (GlcNAc...) Potential

Disulfide bond

33 ↔ 58

Ref.6

Disulfide bond

45 ↔ 154

Ref.6

Disulfide bond

102 ↔ 112

Ref.6

Natural variations

Natural variant

93

1

S → P.
Corresponds to variant rs5743649 [ dbSNP | Ensembl ].

VAR_024531

Natural variant

121

1

Y → C.
Corresponds to variant rs5743651 [ dbSNP | Ensembl ].

VAR_050029

Natural variant

160

1

M → V.
Corresponds to variant rs1802323 [ dbSNP | Ensembl ].

VAR_014539

Secondary structure

1

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162

Helix Strand Turn

Details...

Beta strand

30 – 33

4

Beta strand

35 – 37

3

Beta strand

39 – 44

6

Beta strand

52 – 57

6

Beta strand

67 – 73

7

Beta strand

79 – 89

11

Beta strand

92 – 102

11

Turn

110 – 113

4

Beta strand

119 – 125

7

Beta strand

135 – 145

11

Beta strand

151 – 161

11

Sequences

	Sequence		Length	Mass (Da)
	O95711 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3E6497E2DB4C6F27	FASTA	162	17,906
	10 20 30 40 50 60 MKGFTATLFL WTLIFPSCSG GGGGKAWPTH VVCSDSGLEV LYQSCDPLQD FGFSVEKCSK 70 80 90 100 110 120 QLKSNINIRF GIILREDIKE LFLDLALMSQ GSSVLNFSYP ICEAALPKFS FCGRRKGEQI 130 140 150 160 YYAGPVNNPE FTIPQGEYQV LLELYTEKRS TVACANATIM CS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RP105 is associated with MD-1 and transmits an activation signal in human B cells." Miura Y., Shimazu R., Miyake K., Akashi S., Ogata H., Yamashita Y., Narisawa Y., Kimoto M. Blood 92:2815-2822(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver and Spleen.
[2]	"Human MD-1 homologue is a BCG-regulated gene product in monocytes: Its identification by differential display." Begum N.A., Tsuji S., Nomura M., Shida K., Azuma I., Hayashi A., Matsumoto M., Seya T., Toyoshima K. Biochem. Biophys. Res. Commun. 256:325-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-162. Tissue: Monocyte.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-35.
[6]	"Crystal structures of mouse and human RP105/MD-1 complexes reveal unique dimer organization of the toll-like receptor family." Ohto U., Miyake K., Shimizu T. J. Mol. Biol. 413:815-825(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 21-162 IN COMPLEX WITH CD180, SUBUNIT, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF057178 mRNA. Translation: AAC98152.2.
AB020499 mRNA. Translation: BAA76410.1. Different initiation.
AL031123 Genomic DNA. Translation: CAB76262.1.
BC038846 mRNA. Translation: AAH38846.1.

IPI

IPI00003564.

RefSeq

NP_004262.1. NM_004271.3.

UniGene

Hs.653138.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B2D	X-ray	2.80	C/D	21-162	[»]

ProteinModelPortal

O95711.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59105N.

STRING

9606.ENSP00000230568.

Proteomic databases

PRIDE

O95711.

Protocols and materials databases

DNASU

9450.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000230568; ENSP00000230568; ENSG00000112799.
ENST00000379953; ENSP00000369286; ENSG00000112799.

GeneID

9450.

KEGG

hsa:9450.

UCSC

uc003mwy.1. human.

Organism-specific databases

CTD

9450.

GeneCards

GC06P006588.

HGNC

HGNC:16837. LY86.

HPA

CAB025000.

MIM

605241. gene.

neXtProt

NX_O95711.

PharmGKB

PA128394549.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41851.

HOGENOM

HOG000261640.

HOVERGEN

HBG019056.

InParanoid

O95711.

OMA

VACANAT.

OrthoDB

EOG47SSG4.

PhylomeDB

O95711.

Gene expression databases

Bgee

O95711.

CleanEx

HS_LY86.

Genevestigator

O95711.

GermOnline

ENSG00000112799. Homo sapiens.

Family and domain databases

InterPro

IPR014756. Ig_E-set.
IPR003172. MD-2_lipid-recog_dom.
[Graphical view]

Pfam

PF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]

SMART

SM00737. ML. 1 hit.
[Graphical view]

SUPFAM

SSF81296. Ig_E-set. 1 hit.

ProtoNet

Search...

Other

GenomeRNAi

9450.

NextBio

35400.

SOURCE

Search...

Entry information

Entry name

LY86_HUMAN

Accession

Primary (citable) accession number: O95711
Secondary accession number(s): Q9UQC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.