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Protein

Lymphocyte antigen 86

Gene

LY86

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression (By similarity).By similarity

GO - Biological processi

  1. apoptotic process Source: ProtInc
  2. cell proliferation Source: ProtInc
  3. humoral immune response Source: ProtInc
  4. inflammatory response Source: UniProtKB-KW
  5. innate immune response Source: UniProtKB-KW
  6. positive regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_6894. Toll Like Receptor 4 (TLR4) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte antigen 86
Short name:
Ly-86
Alternative name(s):
Protein MD-1
Gene namesi
Name:LY86
Synonyms:MD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:16837. LY86.

Subcellular locationi

Secretedextracellular space
Note: Associated with CD180 at the cell surface.

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
  2. plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394549.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 20201 PublicationAdd
BLAST
Chaini21 – 162142Lymphocyte antigen 86PRO_0000018614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 581 Publication
Disulfide bondi45 ↔ 1541 Publication
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi102 ↔ 1121 Publication
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO95711.

Expressioni

Tissue specificityi

Highly expressed in B-cells, monocytes and tonsil.

Inductioni

In monocytes, down-regulated by the cell-wall fraction of Mycobacterium bovis (BCG-CWS).

Gene expression databases

BgeeiO95711.
CleanExiHS_LY86.
GenevestigatoriO95711.

Organism-specific databases

HPAiCAB025000.
HPA044895.

Interactioni

Subunit structurei

M-shaped tetramer of two CD180-LY86 heterodimers.1 Publication

Protein-protein interaction databases

BioGridi114839. 2 interactions.
DIPiDIP-59105N.
STRINGi9606.ENSP00000230568.

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 334Combined sources
Beta strandi35 – 373Combined sources
Beta strandi39 – 446Combined sources
Beta strandi52 – 576Combined sources
Beta strandi67 – 737Combined sources
Beta strandi79 – 8911Combined sources
Beta strandi92 – 10211Combined sources
Turni110 – 1134Combined sources
Beta strandi119 – 1257Combined sources
Beta strandi135 – 14511Combined sources
Beta strandi151 – 16111Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B2DX-ray2.80C/D21-162[»]
ProteinModelPortaliO95711.
SMRiO95711. Positions 26-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG41851.
GeneTreeiENSGT00390000018605.
HOGENOMiHOG000261640.
HOVERGENiHBG019056.
InParanoidiO95711.
OMAiINIRFGI.
OrthoDBiEOG7T4MMZ.
PhylomeDBiO95711.
TreeFamiTF335876.

Family and domain databases

InterProiIPR014756. Ig_E-set.
IPR003172. ML_dom.
[Graphical view]
PfamiPF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]
SMARTiSM00737. ML. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGFTATLFL WTLIFPSCSG GGGGKAWPTH VVCSDSGLEV LYQSCDPLQD
60 70 80 90 100
FGFSVEKCSK QLKSNINIRF GIILREDIKE LFLDLALMSQ GSSVLNFSYP
110 120 130 140 150
ICEAALPKFS FCGRRKGEQI YYAGPVNNPE FTIPQGEYQV LLELYTEKRS
160
TVACANATIM CS
Length:162
Mass (Da):17,906
Last modified:May 1, 1999 - v1
Checksum:i3E6497E2DB4C6F27
GO

Sequence cautioni

The sequence BAA76410.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti93 – 931S → P.
Corresponds to variant rs5743649 [ dbSNP | Ensembl ].
VAR_024531
Natural varianti121 – 1211Y → C.
Corresponds to variant rs5743651 [ dbSNP | Ensembl ].
VAR_050029
Natural varianti160 – 1601M → V.
Corresponds to variant rs1802323 [ dbSNP | Ensembl ].
VAR_014539

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057178 mRNA. Translation: AAC98152.2.
AB020499 mRNA. Translation: BAA76410.1. Different initiation.
AL031123 Genomic DNA. Translation: CAB76262.1.
BC038846 mRNA. Translation: AAH38846.1.
CCDSiCCDS4498.1.
RefSeqiNP_004262.1. NM_004271.3.
UniGeneiHs.653138.

Genome annotation databases

EnsembliENST00000230568; ENSP00000230568; ENSG00000112799.
ENST00000379953; ENSP00000369286; ENSG00000112799.
GeneIDi9450.
KEGGihsa:9450.
UCSCiuc003mwy.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057178 mRNA. Translation: AAC98152.2.
AB020499 mRNA. Translation: BAA76410.1. Different initiation.
AL031123 Genomic DNA. Translation: CAB76262.1.
BC038846 mRNA. Translation: AAH38846.1.
CCDSiCCDS4498.1.
RefSeqiNP_004262.1. NM_004271.3.
UniGeneiHs.653138.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B2DX-ray2.80C/D21-162[»]
ProteinModelPortaliO95711.
SMRiO95711. Positions 26-162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114839. 2 interactions.
DIPiDIP-59105N.
STRINGi9606.ENSP00000230568.

Proteomic databases

PRIDEiO95711.

Protocols and materials databases

DNASUi9450.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230568; ENSP00000230568; ENSG00000112799.
ENST00000379953; ENSP00000369286; ENSG00000112799.
GeneIDi9450.
KEGGihsa:9450.
UCSCiuc003mwy.1. human.

Organism-specific databases

CTDi9450.
GeneCardsiGC06P006588.
HGNCiHGNC:16837. LY86.
HPAiCAB025000.
HPA044895.
MIMi605241. gene.
neXtProtiNX_O95711.
PharmGKBiPA128394549.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41851.
GeneTreeiENSGT00390000018605.
HOGENOMiHOG000261640.
HOVERGENiHBG019056.
InParanoidiO95711.
OMAiINIRFGI.
OrthoDBiEOG7T4MMZ.
PhylomeDBiO95711.
TreeFamiTF335876.

Enzyme and pathway databases

ReactomeiREACT_6894. Toll Like Receptor 4 (TLR4) Cascade.

Miscellaneous databases

GenomeRNAii9450.
NextBioi35400.
PROiO95711.
SOURCEiSearch...

Gene expression databases

BgeeiO95711.
CleanExiHS_LY86.
GenevestigatoriO95711.

Family and domain databases

InterProiIPR014756. Ig_E-set.
IPR003172. ML_dom.
[Graphical view]
PfamiPF02221. E1_DerP2_DerF2. 1 hit.
[Graphical view]
SMARTiSM00737. ML. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RP105 is associated with MD-1 and transmits an activation signal in human B cells."
    Miura Y., Shimazu R., Miyake K., Akashi S., Ogata H., Yamashita Y., Narisawa Y., Kimoto M.
    Blood 92:2815-2822(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal liver and Spleen.
  2. "Human MD-1 homologue is a BCG-regulated gene product in monocytes: Its identification by differential display."
    Begum N.A., Tsuji S., Nomura M., Shida K., Azuma I., Hayashi A., Matsumoto M., Seya T., Toyoshima K.
    Biochem. Biophys. Res. Commun. 256:325-329(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-162.
    Tissue: Monocyte.
  3. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
    Zhang Z., Henzel W.J.
    Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-35.
  6. "Crystal structures of mouse and human RP105/MD-1 complexes reveal unique dimer organization of the toll-like receptor family."
    Ohto U., Miyake K., Shimizu T.
    J. Mol. Biol. 413:815-825(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 21-162 IN COMPLEX WITH CD180, SUBUNIT, DISULFIDE BONDS.

Entry informationi

Entry nameiLY86_HUMAN
AccessioniPrimary (citable) accession number: O95711
Secondary accession number(s): Q9UQC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.