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Reviewed, UniProtKB/Swiss-Prot O95674 (CDS2_HUMAN)

Last modified June 16, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Phosphatidate cytidylyltransferase 2
    EC=2.7.7.41
Alternative name(s):
    CDP-diglyceride pyrophosphorylase 2
    CDP-diglyceride synthetase 2
    CDP-diacylglycerol synthase 2
      Short name=CDS 2
    CTP:phosphatidate cytidylyltransferase 2
    CDP-DG synthetase 2
    CDP-DAG synthase 2
Gene names
Name: CDS2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol, phosphatidylglycerol, and cardiolipin.

Catalytic activity

CTP + phosphatidate = diphosphate + CDP-diacylglycerol.

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side Potential.

Tissue specificity

Widely expressed. Expressed in heart, brain and retina, and to a lesser extent in placenta, lung, liver, skeletal muscle, kidney and pancreas. Ref.1

Developmental stage

No expression detected at E10.5 in the developing brain. At E12.5 expression is detected in the developing sentral nervous system and peripheral nervous system. At E17.5 high levels of expression are detected in a number of sites, including the dorsal root ganglia of the peripheral nervous system and the ganglion cell layer of the neural retina in the developing eye. At this stage expression in the brain is restricted to the ventricular zone of the telencephalon, in particular in the basal ganglia and the cerebral cortex. Ref.1

Sequence similarities

Belongs to the CDS family.

Ontologies

Keywords
   Biological processPhospholipid biosynthesis
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
   Molecular functionNucleotidyltransferase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processphospholipid biosynthetic process

Non-traceable author statement. Source: UniProtKB

   Cellular componentintegral to membrane

Non-traceable author statement. Source: UniProtKB

mitochondrial inner membrane

Non-traceable author statement. Source: UniProtKB

   Molecular functionphosphatidate cytidylyltransferase activity Ref.4

Non-traceable author statement. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95674-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95674-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.
     56-64: NRALSNLSS → MSRLRHPRT
     306-445: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Phosphatidate cytidylyltransferase 2
PRO_0000090716

Regions

Transmembrane79 – 9921 Potential
Transmembrane132 – 15221 Potential
Transmembrane166 – 18621 Potential
Transmembrane213 – 23321 Potential
Transmembrane262 – 28221 Potential
Transmembrane340 – 36021 Potential

Amino acid modifications

Modified residue211Phosphoserine Ref.5 Ref.7
Modified residue231Phosphoserine Ref.8
Modified residue311Phosphothreonine By similarity
Modified residue331Phosphoserine Ref.7 Ref.8 Ref.6
Modified residue371Phosphoserine Ref.7
Modified residue471Phosphoserine By similarity

Natural variations

Alternative sequence1 – 5555Missing in isoform 2.
VSP_015477
Alternative sequence56 – 649NRALSNLSS → MSRLRHPRT in isoform 2.
VSP_015480
Alternative sequence306 – 445140Missing in isoform 2.
VSP_015482

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 7DAD6A3C1D5587D6

FASTA44551,418
        10         20         30         40         50         60 
MTELRQRVAH EPVAPPEDKE SESEAKVDGE TASDSESRAE SAPLPVSADD TPEVLNRALS 

        70         80         90        100        110        120 
NLSSRWKNWW VRGILTLAMI AFFFIIIYLG PMVLMIIVMC VQIKCFHEII TIGYNVYHSY 

       130        140        150        160        170        180 
DLPWFRTLSW YFLLCVNYFF YGETVTDYFF TLVQREEPLR ILSKYHRFIS FTLYLIGFCM 

       190        200        210        220        230        240 
FVLSLVKKHY RLQFYMFGWT HVTLLIVVTQ SHLVIHNLFE GMIWFIVPIS CVICNDIMAY 

       250        260        270        280        290        300 
MFGFFFGRTP LIKLSPKKTW EGFIGGFFAT VVFGLLLSYV MSGYRCFVCP VEYNNDTNSF 

       310        320        330        340        350        360 
TVDCEPSDLF RLQEYNIPGV IQSVIGWKTV RMYPFQIHSI ALSTFASLIG PFGGFFASGF 

       370        380        390        400        410        420 
KRAFKIKDFA NTIPGHGGIM DRFDCQYLMA TFVNVYIASF IRGPNPSKLI QQFLTLRPDQ 

       430        440 
QLHIFNTLRS HLIDKGMLTS TTEDE 

« Hide

Isoform 2.

Checksum: 5482E813ADA4239C
Show »

FASTA25029,890

References

« Hide 'large scale' references
[1]"Identification and characterization of CDS2, a mammalian homolog of the Drosophila CDP-diacylglycerol synthase gene."
Volta M., Bulfone A., Gattuso C., Rossi E., Mariani M., Consalez G.G., Zuffardi O., Ballabio A., Banfi S., Franco B.
Genomics 55:68-77(1999) [PubMed: 9889000] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed: 11780052] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"Isolation and chromosomal localization of two human CDP-diacylglycerol synthase (CDS) genes."
Halford S., Dulai K.S., Daw S.C.M., Fitzgibbon J., Hunt D.M.
Genomics 54:140-144(1998) [PubMed: 9806839] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-445 (ISOFORM 1).
Tissue: Retina.
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
Tissue: Epithelium.
[6]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY.
Tissue: Platelet.
[7]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-33 AND SER-37, MASS SPECTROMETRY.
[8]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-33, MASS SPECTROMETRY.
Tissue: Liver.

Cross-references

Sequence databases

Y16521 mRNA. Translation: CAA76270.1.
AL121755, AL121924 Genomic DNA. Translation: CAI22376.1.
AL121755, AL121924 Genomic DNA. Translation: CAI22378.1.
AL121924, AL121755 Genomic DNA. Translation: CAI22631.1.
AL121924, AL121755 Genomic DNA. Translation: CAI22633.1.
BC025751 mRNA. Translation: AAH25751.1.
AF069532 mRNA. Translation: AAC78305.1.
IPIIPI00032150.
IPI00646081.
RefSeqNP_003809.1.
UniGeneHs.714354

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteO95674.

Proteomic databases

PRIDEO95674.

Genome annotation databases

EnsemblENSG00000101290. Homo sapiens. [Contig view]
GeneID8760.
KEGGhsa:8760.
NMPDRfig|9606.3.peg.19834.

Organism-specific databases

GeneCardsGC20P005055.
HGNCHGNC:1801. CDS2.
HPAHPA019698.
MIM603549. gene.
PharmGKBPA26347.
GenAtlasSearch...

Phylogenomic databases

HOVERGENO95674.
OMAO95674. PDQQLHI.

Enzyme and pathway databases

BRENDA2.7.7.41. 247.

Gene expression databases

ArrayExpressO95674.
BgeeO95674.
CleanExHS_CDS2.
GermOnlineENSG00000101290. Homo sapiens.

Family and domain databases

InterProIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PfamPF01148. CTP_transf_1. 1 hit.
[Graphical view]
PIRSFPIRSF018269. PC_trans_euk. 1 hit.
PROSITEPS01315. CDS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio32860.
SOURCESearch...

Entry information

Entry nameCDS2_HUMAN
AccessionPrimary (citable) accession number: O95674
Secondary accession number(s): Q5TDY2 expand/collapse secondary AC list , Q5TDY3, Q5TDY4, Q5TDY5, Q9BYK5, Q9NTT2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents