Reviewed, UniProtKB/Swiss-Prot O95633 (FSTL3_HUMAN)

Names and origin

Protein names

Recommended name:
    Follistatin-related protein 3
Alternative name(s):
    Follistatin-like 3
    Follistatin-related gene protein

Gene names

Name:	FSTL3
Synonyms:	FLRG
ORF Names:	UNQ674/PRO1308

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binding protein for activin and BMP2 By similarity.
Subcellular location	SecretedPotential. Nucleus. Note= According to Ref.5 it is nuclear.
Tissue specificity	Expressed in a wide range of tissues.
Involvement in disease	A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1.
Sequence similarities	Contains 2 follistatin-like domains. Contains 2 Kazal-like domains. Contains 1 TB (TGF-beta binding) domain.

Ontologies

Keywords
Cellular component	Nucleus Secreted
Coding sequence diversity	Chromosomal rearrangement
Disease	Proto-oncogene
Domain	Repeat Signal
PTM	Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular space Ref.1 Traceable author statement. Source: ProtInc nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 263	237	Follistatin-related protein 3		PRO_0000010115
Regions
	Domain	36 – 107	72	TB
	Domain	121 – 167	47	Kazal-like 1
	Domain	170 – 193	24	Follistatin-like 2
	Domain	195 – 243	49	Kazal-like 2
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Disulfide bond	121 ↔ 153		By similarity
	Disulfide bond	125 ↔ 146		By similarity
	Disulfide bond	135 ↔ 167		By similarity
	Disulfide bond	195 ↔ 229		By similarity
	Disulfide bond	200 ↔ 222		By similarity
	Disulfide bond	211 ↔ 243		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O95633-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6A9AB86ADD4FD09C	FASTA	263	27,663
	10 20 30 40 50 60 MRPGAPGPLW PLPWGALAWA VGFVSSMGSG NPAPGGVCWL QQGQEATCSL VLQTDVTRAE 70 80 90 100 110 120 CCASGNIDTA WSNLTHPGNK INLLGFLGLV HCLPCKDSCD GVECGPGKAC RMLGGRPRCE 130 140 150 160 170 180 CAPDCSGLPA RLQVCGSDGA TYRDECELRA ARCRGHPDLS VMYRGRCRKS CEHVVCPRPQ 190 200 210 220 230 240 SCVVDQTGSA HCVVCRAAPC PVPSSPGQEL CGNNNVTYIS SCHMRQATCF LGRSIGVRHA 250 260 GSCAGTPEEP PGGESAEEEE NFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FLRG (follistatin-related gene), a new target of chromosomal rearrangement in malignant blood disorders." Hayette S., Gadoux M., Martel S., Bertrand S., Tigaud I., Magaud J.-P., Rimokh R. Oncogene 16:2949-2954(1998) [PubMed: 9671416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION. Tissue: Placenta.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[5]	"Human follistatin-related protein: a structural homologue of follistatin with nuclear localization." Tortoriello D.V., Sidis Y., Holtzman D.A., Holmes W.E., Schneyer A.L. Endocrinology 142:3426-3434(2001) [PubMed: 11459787] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U76702 mRNA. Translation: AAC64321.1.
AY358917 mRNA. Translation: AAQ89276.1.
BC005839 mRNA. Translation: AAH05839.1.
BC033119 mRNA. Translation: AAH33119.1.

RefSeq

NP_005851.1.

UniGene

Hs.529038

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B4V	X-ray	2.48	C/D/G/H	27-263	[»]

ModBase

Search...

Protein family/group databases

MEROPS

I01.968.

Proteomic databases

PeptideAtlas

O95633.

Genome annotation databases

Ensembl

ENSG00000070404. Homo sapiens. [Contig view]

GeneID

10272.

KEGG

hsa:10272.

Organism-specific databases

H-InvDB

HIX0014560.

HGNC

HGNC:3973. FSTL3.

MIM

605343. gene.

Orphanet

67038. B-cell chronic lymphocytic leukaemia.

PharmGKB

PA28390.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

O95633.

HOVERGEN

O95633.

Gene expression databases

ArrayExpress

O95633.

CleanEx

HS_FSTL3.

GermOnline

ENSG00000070404. Homo sapiens.

Family and domain databases

InterPro

IPR003645. Fol_N.
IPR015204. Fol_N_sub.
IPR002350. Prot_inh_Kazal.
IPR011497. Prot_Inh_Kazal_2.
[Graphical view]

Pfam

PF09120. EGF-like_subdom. 1 hit.
PF00050. Kazal_1. 1 hit.
PF07648. Kazal_2. 1 hit.
[Graphical view]

SMART

SM00274. FOLN. 2 hits.
SM00280. KAZAL. 2 hits.
[Graphical view]

PROSITE

PS00282. KAZAL. False negative.
PS51364. TB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

38914.

SOURCE

Search...

Entry information

Entry name

FSTL3_HUMAN

Accession

Primary (citable) accession number: O95633

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	May 1, 1999
Last modified:	November 25, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)