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O95631 (NET1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Netrin-1
Alternative name(s):
Epididymis tissue protein Li 131P
Gene names
Name:NTN1
Synonyms:NTN1L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis. Ref.4

Subunit structure

Binds to its receptors; DCC, DSCAM, UNC5A, UNC5B, UNC5C and probably UNC5D. Ref.1 Ref.5

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level). Ref.1 Ref.3

Sequence similarities

Contains 3 laminin EGF-like domains.

Contains 1 laminin N-terminal domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processApoptosis
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityPolymorphism
   DomainLaminin EGF-like domain
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior axon guidance

Inferred from electronic annotation. Source: Ensembl

apoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

axon guidance

Traceable author statement. Source: Reactome

cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

establishment of nucleus localization

Inferred from electronic annotation. Source: Ensembl

inner ear morphogenesis

Inferred from electronic annotation. Source: Ensembl

mammary gland duct morphogenesis

Inferred from electronic annotation. Source: Ensembl

negative regulation of axon extension

Inferred from electronic annotation. Source: Ensembl

neuron migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of axon extension

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasement membrane

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular region

Traceable author statement. Source: Reactome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 604580Netrin-1
PRO_0000017082

Regions

Domain47 – 284238Laminin N-terminal
Domain285 – 34056Laminin EGF-like 1
Domain341 – 40363Laminin EGF-like 2
Domain404 – 45350Laminin EGF-like 3
Domain472 – 601130NTR
Motif530 – 5323Cell attachment site Potential

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation4171N-linked (GlcNAc...) Potential
Disulfide bond119 ↔ 152 By similarity
Disulfide bond285 ↔ 294 By similarity
Disulfide bond287 ↔ 304 By similarity
Disulfide bond306 ↔ 315 By similarity
Disulfide bond318 ↔ 338 By similarity
Disulfide bond341 ↔ 350 By similarity
Disulfide bond343 ↔ 368 By similarity
Disulfide bond371 ↔ 380 By similarity
Disulfide bond383 ↔ 401 By similarity
Disulfide bond404 ↔ 416 By similarity
Disulfide bond406 ↔ 423 By similarity
Disulfide bond425 ↔ 434 By similarity
Disulfide bond437 ↔ 451 By similarity
Disulfide bond472 ↔ 544 By similarity
Disulfide bond491 ↔ 601 By similarity

Natural variations

Natural variant3511R → H in a neuroblastoma sample. Ref.1
VAR_014279
Natural variant4891K → E in a neuroblastoma sample. Ref.1
VAR_014280

Experimental info

Sequence conflict2991D → T in AAD09221. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O95631 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 9827C09D0D783B27

FASTA60467,748
        10         20         30         40         50         60 
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF 

        70         80         90        100        110        120 
GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNASDPK KAHPPAFLTD LNNPHNLTCW 

       130        140        150        160        170        180 
QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ 

       190        200        210        220        230        240 
CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD 

       250        260        270        280        290        300 
WVTATDIRVA FSRLHTFGDE NEDDSELARD SYFYAVSDLQ VGGRCKCNGH AARCVRDRDD 

       310        320        330        340        350        360 
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS 

       370        380        390        400        410        420 
GRKSGGVCLN CRHNTAGRHC HYCKEGYYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT 

       430        440        450        460        470        480 
GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK 

       490        500        510        520        530        540 
GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR 

       550        560        570        580        590        600 
DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK 


CKKA 

« Hide

References

« Hide 'large scale' references
[1]"Netrin-1: interaction with deleted in colorectal cancer (DCC) and alterations in brain tumors and neuroblastomas."
Meyerhardt J.A., Caca K., Eckstrand B.C., Hu G., Lengauer C., Banavali S., Look A.T., Fearon E.R.
Cell Growth Differ. 10:35-42(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH DCC, TISSUE SPECIFICITY, VARIANTS HIS-351 AND GLU-489.
Tissue: Brain stem and Liver.
[2]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins."
Li J., Liu F., Wang H., Liu X., Liu J., Li N., Wan F., Wang W., Zhang C., Jin S., Liu J., Zhu P., Liu Y.
Mol. Cell. Proteomics 9:2517-2528(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Epididymis.
[4]"Netrin-1 controls colorectal tumorigenesis by regulating apoptosis."
Mazelin L., Bernet A., Bonod-Bidaud C., Pays L., Arnaud S., Gespach C., Bredesen D.E., Scoazec J.-Y., Mehlen P.
Nature 431:80-84(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"DSCAM functions as a netrin receptor in commissural axon pathfinding."
Liu G., Li W., Wang L., Kar A., Guan K.L., Rao Y., Wu J.Y.
Proc. Natl. Acad. Sci. U.S.A. 106:2951-2956(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DSCAM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U75586 mRNA. Translation: AAD09221.1.
GU727649 mRNA. Translation: ADU87650.1.
AC090610 Genomic DNA. No translation available.
AC005695 Genomic DNA. No translation available.
RefSeqNP_004813.2. NM_004822.2.
UniGeneHs.660885.

3D structure databases

ProteinModelPortalO95631.
SMRO95631. Positions 41-483, 488-583.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114816. 2 interactions.
DIPDIP-46273N.
IntActO95631. 1 interaction.
STRING9606.ENSP00000173229.

PTM databases

PhosphoSiteO95631.

Proteomic databases

PaxDbO95631.
PRIDEO95631.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000173229; ENSP00000173229; ENSG00000065320.
ENST00000538852; ENSP00000443259; ENSG00000065320.
ENST00000546090; ENSP00000441611; ENSG00000065320.
GeneID9423.
KEGGhsa:9423.
UCSCuc002glw.4. human.

Organism-specific databases

CTD9423.
GeneCardsGC17P008924.
H-InvDBHIX0039241.
HGNCHGNC:8029. NTN1.
HPAHPA056419.
MIM601614. gene.
neXtProtNX_O95631.
PharmGKBPA31813.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG244580.
HOGENOMHOG000286017.
HOVERGENHBG006464.
InParanoidO95631.
KOK06843.
OMADSYYYAV.
OrthoDBEOG7SR4KJ.
PhylomeDBO95631.
TreeFamTF352481.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
REACT_111155. Cell-Cell communication.

Gene expression databases

ArrayExpressO95631.
BgeeO95631.
CleanExHS_NTN1.
GenevestigatorO95631.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
InterProIPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR008979. Galactose-bd-like.
IPR008211. Laminin_N.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PfamPF00053. Laminin_EGF. 3 hits.
PF00055. Laminin_N. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00180. EGF_Lam. 3 hits.
SM00136. LamNT. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF50242. SSF50242. 1 hit.
PROSITEPS00022. EGF_1. 2 hits.
PS01248. EGF_LAM_1. 3 hits.
PS50027. EGF_LAM_2. 3 hits.
PS51117. LAMININ_NTER. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNTN1.
GenomeRNAi9423.
NextBio35298.
PROO95631.
SOURCESearch...

Entry information

Entry nameNET1_HUMAN
AccessionPrimary (citable) accession number: O95631
Secondary accession number(s): E9KL51
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 5, 2009
Last modified: March 19, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM