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Protein

Netrin-1

Gene

NTN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.1 Publication

GO - Biological processi

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-HSA-373752 Netrin-1 signaling
R-HSA-376172 DSCAM interactions
R-HSA-418885 DCC mediated attractive signaling
R-HSA-418886 Netrin mediated repulsion signals
R-HSA-418890 Role of second messengers in netrin-1 signaling
R-HSA-428542 Regulation of commissural axon pathfinding by SLIT and ROBO
SIGNORiO95631

Names & Taxonomyi

Protein namesi
Recommended name:
Netrin-1
Alternative name(s):
Epididymis tissue protein Li 131P
Gene namesi
Name:NTN1
Synonyms:NTN1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000065320.8
HGNCiHGNC:8029 NTN1
MIMi601614 gene
neXtProtiNX_O95631

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi9423
MalaCardsiNTN1
OpenTargetsiENSG00000065320
PharmGKBiPA31813

Polymorphism and mutation databases

BioMutaiNTN1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001708225 – 604Netrin-1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi119 ↔ 152By similarity
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi285 ↔ 294By similarity
Disulfide bondi287 ↔ 304By similarity
Disulfide bondi306 ↔ 315By similarity
Disulfide bondi318 ↔ 338By similarity
Disulfide bondi341 ↔ 350By similarity
Disulfide bondi343 ↔ 368By similarity
Disulfide bondi371 ↔ 380By similarity
Disulfide bondi383 ↔ 401By similarity
Disulfide bondi404 ↔ 416By similarity
Disulfide bondi406 ↔ 423By similarity
Glycosylationi417N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi425 ↔ 434By similarity
Disulfide bondi437 ↔ 451By similarity
Disulfide bondi472 ↔ 544By similarity
Disulfide bondi491 ↔ 601By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO95631
PaxDbiO95631
PeptideAtlasiO95631
PRIDEiO95631

PTM databases

iPTMnetiO95631
PhosphoSitePlusiO95631

Expressioni

Tissue specificityi

Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000065320
CleanExiHS_NTN1
ExpressionAtlasiO95631 baseline and differential
GenevisibleiO95631 HS

Organism-specific databases

HPAiHPA056419

Interactioni

Subunit structurei

Binds to its receptors; DCC, DSCAM, UNC5A, UNC5B, UNC5C and probably UNC5D.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114816, 5 interactors
DIPiDIP-46273N
IntActiO95631, 7 interactors
STRINGi9606.ENSP00000173229

Structurei

Secondary structure

1604
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni58 – 61Combined sources4
Beta strandi75 – 79Combined sources5
Beta strandi83 – 86Combined sources4
Beta strandi90 – 94Combined sources5
Beta strandi96 – 99Combined sources4
Helixi105 – 107Combined sources3
Beta strandi108 – 110Combined sources3
Beta strandi132 – 153Combined sources22
Beta strandi157 – 166Combined sources10
Beta strandi172 – 179Combined sources8
Helixi181 – 184Combined sources4
Helixi197 – 199Combined sources3
Beta strandi202 – 204Combined sources3
Beta strandi210 – 212Combined sources3
Beta strandi217 – 221Combined sources5
Turni222 – 225Combined sources4
Helixi227 – 234Combined sources8
Helixi236 – 241Combined sources6
Beta strandi243 – 251Combined sources9
Beta strandi257 – 259Combined sources3
Turni266 – 268Combined sources3
Helixi269 – 271Combined sources3
Beta strandi275 – 285Combined sources11
Beta strandi294 – 296Combined sources3
Beta strandi302 – 304Combined sources3
Beta strandi310 – 312Combined sources3
Beta strandi348 – 352Combined sources5
Helixi354 – 359Combined sources6
Beta strandi366 – 370Combined sources5
Turni372 – 374Combined sources3
Beta strandi375 – 377Combined sources3
Beta strandi387 – 389Combined sources3
Beta strandi401 – 403Combined sources3
Turni408 – 410Combined sources3
Beta strandi418 – 420Combined sources3
Beta strandi429 – 431Combined sources3
Beta strandi451 – 453Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4URTX-ray3.10A39-453[»]
6FKQX-ray3.07A39-453[»]
ProteinModelPortaliO95631
SMRiO95631
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 284Laminin N-terminalPROSITE-ProRule annotationAdd BLAST238
Domaini285 – 340Laminin EGF-like 1PROSITE-ProRule annotationAdd BLAST56
Domaini341 – 403Laminin EGF-like 2PROSITE-ProRule annotationAdd BLAST63
Domaini404 – 453Laminin EGF-like 3PROSITE-ProRule annotationAdd BLAST50
Domaini472 – 601NTRPROSITE-ProRule annotationAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi530 – 532Cell attachment siteSequence analysis3

Keywords - Domaini

Laminin EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3512 Eukaryota
ENOG410XS7U LUCA
GeneTreeiENSGT00890000139348
HOGENOMiHOG000286017
HOVERGENiHBG006464
InParanoidiO95631
KOiK06843
OMAiDQFLWVR
OrthoDBiEOG091G005L
PhylomeDBiO95631
TreeFamiTF352481

Family and domain databases

Gene3Di2.60.120.1490, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR002049 Laminin_EGF
IPR008211 Laminin_N
IPR038684 Laminin_N_sf
IPR001134 Netrin_domain
IPR018933 Netrin_module_non-TIMP
IPR008993 TIMP-like_OB-fold
PfamiView protein in Pfam
PF00053 Laminin_EGF, 3 hits
PF00055 Laminin_N, 1 hit
PF01759 NTR, 1 hit
SMARTiView protein in SMART
SM00643 C345C, 1 hit
SM00180 EGF_Lam, 3 hits
SM00136 LamNT, 1 hit
SUPFAMiSSF49785 SSF49785, 1 hit
SSF50242 SSF50242, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01248 EGF_LAM_1, 3 hits
PS50027 EGF_LAM_2, 3 hits
PS51117 LAMININ_NTER, 1 hit
PS50189 NTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR
60 70 80 90 100
CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNASDPK
110 120 130 140 150
KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ
160 170 180 190 200
FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE
210 220 230 240 250
AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA
260 270 280 290 300
FSRLHTFGDE NEDDSELARD SYFYAVSDLQ VGGRCKCNGH AARCVRDRDD
310 320 330 340 350
SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC
360 370 380 390 400
RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGYYRD MGKPITHRKA
410 420 430 440 450
CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP
460 470 480 490 500
CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKINMKKY CKKDYAVQIH
510 520 530 540 550
ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK
560 570 580 590 600
PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK

CKKA
Length:604
Mass (Da):67,748
Last modified:May 5, 2009 - v2
Checksum:i9827C09D0D783B27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299D → T in AAD09221 (PubMed:9950216).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_014279351R → H in a neuroblastoma sample. 1 PublicationCorresponds to variant dbSNP:rs531668666Ensembl.1
Natural variantiVAR_014280489K → E in a neuroblastoma sample. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75586 mRNA Translation: AAD09221.1
GU727649 mRNA Translation: ADU87650.1
AC090610 Genomic DNA No translation available.
AC005695 Genomic DNA No translation available.
CCDSiCCDS11148.1
RefSeqiNP_004813.2, NM_004822.2
XP_006721658.1, XM_006721595.3
UniGeneiHs.660885

Genome annotation databases

EnsembliENST00000173229; ENSP00000173229; ENSG00000065320
GeneIDi9423
KEGGihsa:9423
UCSCiuc002glw.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNET1_HUMAN
AccessioniPrimary (citable) accession number: O95631
Secondary accession number(s): E9KL51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 5, 2009
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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