Reviewed, UniProtKB/Swiss-Prot O95631 (NET1_HUMAN)

Names and origin

Protein names

Recommended name:
    Netrin-1

Gene names

Name:	NTN1
Synonyms:	NTN1L

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Ref.3
Subunit structure	Binds to its receptors; DCC, UNC5A, UNC5B, UNC5C and probably UNC5D. Ref.1
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Ref.1
Involvement in disease	Defects in NTN1 are associated with some forms of neuroblastomas.
Sequence similarities	Contains 3 laminin EGF-like domains. Contains 1 laminin N-terminal domain. Contains 1 NTR domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Extracellular matrix Secreted
Disease	Disease mutation
Domain	Laminin EGF-like domain Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 604	580	Netrin-1		PRO_0000017082
Regions
	Domain	47 – 284	238	Laminin N-terminal
	Domain	285 – 340	56	Laminin EGF-like 1
	Domain	341 – 403	63	Laminin EGF-like 2
	Domain	404 – 453	50	Laminin EGF-like 3
	Domain	472 – 601	130	NTR
	Motif	530 – 532	3	Cell attachment site Potential
Amino acid modifications
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	417	1	N-linked (GlcNAc...) Potential
	Disulfide bond	119 ↔ 152		By similarity
	Disulfide bond	285 ↔ 294		By similarity
	Disulfide bond	287 ↔ 304		By similarity
	Disulfide bond	306 ↔ 315		By similarity
	Disulfide bond	318 ↔ 338		By similarity
	Disulfide bond	341 ↔ 350		By similarity
	Disulfide bond	343 ↔ 368		By similarity
	Disulfide bond	371 ↔ 380		By similarity
	Disulfide bond	383 ↔ 401		By similarity
	Disulfide bond	404 ↔ 416		By similarity
	Disulfide bond	406 ↔ 423		By similarity
	Disulfide bond	425 ↔ 434		By similarity
	Disulfide bond	437 ↔ 451		By similarity
	Disulfide bond	472 ↔ 544		By similarity
	Disulfide bond	491 ↔ 601		By similarity
Natural variations
	Natural variant	351	1	R → H in neuroblastoma. Ref.1		VAR_014279
	Natural variant	489	1	K → E in neuroblastoma. Ref.1		VAR_014280
Experimental info
	Sequence conflict	299	1	D → T in AAD09221. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O95631-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 9827C09D0D783B27	FASTA	604	67,748
	10 20 30 40 50 60 MMRAVWEALA ALAAVACLVG AVRGGPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF 70 80 90 100 110 120 GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNASDPK KAHPPAFLTD LNNPHNLTCW 130 140 150 160 170 180 QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ 190 200 210 220 230 240 CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD 250 260 270 280 290 300 WVTATDIRVA FSRLHTFGDE NEDDSELARD SYFYAVSDLQ VGGRCKCNGH AARCVRDRDD 310 320 330 340 350 360 SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS 370 380 390 400 410 420 GRKSGGVCLN CRHNTAGRHC HYCKEGYYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT 430 440 450 460 470 480 GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK 490 500 510 520 530 540 GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR 550 560 570 580 590 600 DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK CKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Netrin-1: interaction with deleted in colorectal cancer (DCC) and alterations in brain tumors and neuroblastomas." Meyerhardt J.A., Caca K., Eckstrand B.C., Hu G., Lengauer C., Banavali S., Look A.T., Fearon E.R. Cell Growth Differ. 10:35-42(1999) [PubMed: 9950216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH DCC, TISSUE SPECIFICITY, VARIANTS HIS-351 AND GLU-489. Tissue: Brain stem and Liver.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Netrin-1 controls colorectal tumorigenesis by regulating apoptosis." Mazelin L., Bernet A., Bonod-Bidaud C., Pays L., Arnaud S., Gespach C., Bredesen D.E., Scoazec J.-Y., Mehlen P. Nature 431:80-84(2004) [PubMed: 15343335] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U75586 mRNA. Translation: AAD09221.1. AC090610 Genomic DNA. No translation available. AC005695 Genomic DNA. No translation available.
IPI	IPI00006732.
RefSeq	NP_004813.2.
UniGene	Hs.660885
3D structure databases
HSSP	HSSP built from PDB template 1NPE based on UniProtKB P02468.
ModBase	Search...
Protein-protein interaction databases
STRING	O95631.
PTM databases
PhosphoSite	O95631.
Proteomic databases
PRIDE	O95631.
Genome annotation databases
Ensembl	ENST00000173229; ENSP00000173229; ENSG00000065320; Homo sapiens. [Genome view] ENST00000436734; ENSP00000389375; ENSG00000065320; Homo sapiens. [Genome view]
GeneID	9423.
KEGG	hsa:9423.
UCSC	uc002glw.2. human.
Organism-specific databases
CTD	9423.
GeneCards	GC17P008865.
H-InvDB	HIX0039241.
HGNC	HGNC:8029. NTN1.
MIM	601614. gene.
PharmGKB	PA26685.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O95631.
HOVERGEN	O95631.
Gene expression databases
ArrayExpress	O95631.
Bgee	O95631.
CleanEx	HS_NTN1.
Genevestigator	O95631.
GermOnline	ENSG00000065320. Homo sapiens.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR002049. EGF_laminin. IPR008211. Laminin_N. IPR001134. Netrin_domain. IPR018933. Netrin_module_non-TIMP. [Graphical view]
Pfam	PF00053. Laminin_EGF. 3 hits. PF00055. Laminin_N. 1 hit. PF01759. NTR. 1 hit. [Graphical view]
SMART	SM00643. C345C. 1 hit. SM00180. EGF_Lam. 3 hits. SM00136. LamNT. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 2 hits. PS01186. EGF_2. False negative. PS01248. EGF_LAM_1. 3 hits. PS50027. EGF_LAM_2. 3 hits. PS51117. LAMININ_NTER. 1 hit. PS50189. NTR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35298.
SOURCE	Search...

Entry information

Entry name

NET1_HUMAN

Accession

Primary (citable) accession number: O95631

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 5, 2009
Last modified:	October 13, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.