O95628 (CNOT4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCR4-NOT transcription complex subunit 4
EC=6.3.2.-

Alternative name(s):
CCR4-associated factor 4
E3 ubiquitin-protein ligase CNOT4
Potential transcriptional repressor NOT4Hp

Gene names

Name:	CNOT4
Synonyms:	NOT4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has E3 ubiquitin ligase activity. Involved in activation of the JAK/STAT pathway. Ref.8 Ref.14
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex. Binds E2 ubiquitin ligases via its RING domain. Interacts (via RING domain) with UBE2D2. Ref.8 Ref.9
Subcellular location	Cytoplasm Probable. Nucleus Probable.
Post-translational modification	Autoubiquitinated.
Sequence similarities	Contains 1 C3H1-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.
Sequence caution	The sequence AAC72963.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAC72963.1 differs from that shown. Reason: Sequencing errors. The sequence AAD00180.1 differs from that shown. Reason: Frameshift at positions 358 and 400. The sequence BAC11125.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear-transcribed mRNA poly(A) tail shortening Traceable author statement. Source: Reactome protein autoubiquitination Inferred from direct assay Ref.9. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from direct assay Ref.9. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 9 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95628-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95628-2)

The sequence of this isoform differs from the canonical sequence as follows:
     271-274: RYDT → S

Isoform 3 (identifier: O95628-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: O95628-4)

The sequence of this isoform differs from the canonical sequence as follows:
     544-575: EEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA → IPASSGNSLD...LLQSSTLDRH

Note: No experimental confirmation available.

Isoform 5 (identifier: O95628-5)

The sequence of this isoform differs from the canonical sequence as follows:
     419-433: IEKELSVQDQPSLSP → TEPIERKRLAVLKRR
     434-575: Missing.

Isoform 6 (identifier: O95628-6)

The sequence of this isoform differs from the canonical sequence as follows:
     543-575: GEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA → DNSSSIESLN...HLFINCKNFC

Note: No experimental confirmation available.

Isoform 7 (identifier: O95628-7)

The sequence of this isoform differs from the canonical sequence as follows:
     544-575: EEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA → IPASSGNSLD...PTAPFSSLMF

Note: No experimental confirmation available.

Isoform 8 (identifier: O95628-8)

The sequence of this isoform differs from the canonical sequence as follows:
     271-274: RYDT → S
     544-575: EEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA → IPASSGNSLD...LLQSSTLDRH

Note: No experimental confirmation available.

Isoform 9 (identifier: O95628-9)

The sequence of this isoform differs from the canonical sequence as follows:
     271-274: RYDT → S
     543-575: GEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA → DNSSSVESLN...LLQSSTLDRH

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 575

575

CCR4-NOT transcription complex subunit 4

PRO_0000081679

Regions

Domain

109 – 189

81

RRM

Zinc finger

14 – 57

44

RING-type; degenerate

Zinc finger

190 – 217

28

C3H1-type

Coiled coil

68 – 104

37

Potential

Amino acid modifications

Modified residue

71

1

Phosphoserine Ref.10

Modified residue

324

1

Phosphoserine Ref.11 Ref.12 Ref.13

Modified residue

432

1

Phosphoserine Ref.12

Modified residue

490

1

Phosphoserine Ref.12

Natural variations

Alternative sequence

1 – 17

17

Missing in isoform 3.

VSP_009923

Alternative sequence

271 – 274

4

RYDT → S in isoform 2, isoform 8 and isoform 9.

VSP_009924

Alternative sequence

419 – 433

15

IEKEL…PSLSP → TEPIERKRLAVLKRR in isoform 5.

VSP_009925

Alternative sequence

434 – 575

142

Missing in isoform 5.

VSP_009926

Alternative sequence

543 – 575

33

GEEEV…HTTVA → DNSSSIESLNMKEWQDGLRA LLPNININFGGLPNSSSPSN ANHSAPTSNTATTDSLSWDS PGSWTDPAIITGIPASSGNS LDSLQDDNPPHWLKSLQALT EMDGPSAAPSQTHHSAPFST QIPLHRASWNPYPPPSNPSS FHSPPPQIYYRVQHWTAIRQ RGATIRKCRICPTLFSPSQP TTHSSLLISYVLKNPVPDQF FFSLTPDAMRSQGHYHLFIN CKNFC in isoform 6.

VSP_009927

Alternative sequence

543 – 575

33

GEEEV…HTTVA → DNSSSVESLNMKEWQDGLRA LLPNININFGGLPNSSSPSN ANHSAPTSNTATTDSLSWDS PGSWTDPAIITGIPASSGNS LDSLQDDNPPHWLKSLQALT EMDGPSAAPSQTHHSAPFST QIPLHRASWNPYPPPSNPSS FHSPPPGFQTAFRPPSKTPT DLLQSSTLDRH in isoform 9.

VSP_045469

Alternative sequence

544 – 575

32

EEEVK…HTTVA → IPASSGNSLDSLQDDNPPHW LKSLQALTEMDGPSAAPSQT HHSAPFSTQIPLHRASWNPY PPPSNPSSFHSPPPGFQTAF RPPSKTPTDLLQSSTLDRH in isoform 4 and isoform 8.

VSP_009928

Alternative sequence

544 – 575

32

EEEVK…HTTVA → IPASSGNSLDSLQDDNPPHW LKSLQALTEMDGQRCSITDP PQRPLQHTDPAAQSQLESLP SSFKPFQLPLPTPRLSDGLQ TPQQNPHRFTTEFNTGPPLG KEEQPLENAGFVPLCFLPLS PPPTAPFSSLMF in isoform 7.

VSP_009929

Natural variant

7

1

A → G. Ref.3
Corresponds to variant rs17480616 [ dbSNP | Ensembl ].

VAR_027833

Experimental info

Mutagenesis

16

1

L → A or E: Abolishes interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

17

1

C → A: Abolishes interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

18

1

M → A: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

33

1

C → R: Abolishes interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

42

1

W → A: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

44

1

R → A or E: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

45

1

I → A or W: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

49

1

E → A: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8 Ref.9

Mutagenesis

49

1

E → K: Strongly reduced interaction with UBE2D2. Ref.8 Ref.9

Mutagenesis

54

1

P → A: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Mutagenesis

57

1

R → A or E: Strongly reduces interaction with E2 ubiquitin ligases. Ref.8

Sequence conflict

178

1

N → D in BAH13270. Ref.4

Sequence conflict

201

1

K → R in BAH13270. Ref.4

Sequence conflict

340

1

N → I in AAH35590. Ref.6

Sequence conflict

570

1

L → F in AAD00179. Ref.1

Sequence conflict

570

1

L → F in AAD00180. Ref.1

Isoform 9:

Sequence conflict

545

1

V → I in BAH13270. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

575

Helix Strand Turn

Details...

Turn

15 – 17

3

Turn

23 – 27

5

Helix

39 – 45

7

Turn

54 – 56

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 3. Checksum: C41762D4EC4222BC	FASTA	575	63,510
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP 310 320 330 340 350 360 SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT 370 380 390 400 410 420 SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE 430 440 450 460 470 480 KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ 490 500 510 520 530 540 QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP 550 560 570 VAGEEEVKVS TMPLSTSSHS LQQGQQPTSL HTTVA
	Isoform 2 [UniParc]. Checksum: 717835C74D66B540	FASTA	572	63,062
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ SPIDKPSDSL SIGNGDNSQQ ISNSDTPSPP 310 320 330 340 350 360 PGLSKSNPVI PISSSNHSAR SPFEGAVTES QSLFSDNFRH PNPIPSGLPP FPSSPQTSSD 370 380 390 400 410 420 WPTAPEPQSL FTSETIPVSS STDWQAAFGF GSSKQPEDDL GFDPFDVTRK ALADLIEKEL 430 440 450 460 470 480 SVQDQPSLSP TSLQNSSSHT TTAKGPGSGF LHPAAATNAN SLNSTFSVLP QRFPQFQQHR 490 500 510 520 530 540 AVYNSFSFPG QAARYPWMAF PRNSIMHLNH TANPTSNSNF LDLNLPPQHN TGLGGIPVAG 550 560 570 EEEVKVSTMP LSTSSHSLQQ GQQPTSLHTT VA
	Isoform 3 [UniParc]. Checksum: DAE0356900B05FE4	FASTA	558	61,651
	10 20 30 40 50 60 MEPLEIDDIN FFPCTCGYQI CRFCWHRIRT DENGLCPACR KPYPEDPAVY KPLSQEELQR 70 80 90 100 110 120 IKNEKKQKQN ERKQKISENR KHLASVRVVQ KNLVFVVGLS QRLADPEVLK RPEYFGKFGK 130 140 150 160 170 180 IHKVVINNST SYAGSQGPSA SAYVTYIRSE DALRAIQCVN NVVVDGRTLK ASLGTTKYCS 190 200 210 220 230 240 YFLKNMQCPK PDCMYLHELG DEAASFTKEE MQAGKHQEYE QKLLQELYKL NPNFLQLSTG 250 260 270 280 290 300 SVDKNKNKVT PLQRYDTPID KPSDSLSIGN GDNSQQISNS DTPSPPPGLS KSNPVIPISS 310 320 330 340 350 360 SNHSARSPFE GAVTESQSLF SDNFRHPNPI PSGLPPFPSS PQTSSDWPTA PEPQSLFTSE 370 380 390 400 410 420 TIPVSSSTDW QAAFGFGSSK QPEDDLGFDP FDVTRKALAD LIEKELSVQD QPSLSPTSLQ 430 440 450 460 470 480 NSSSHTTTAK GPGSGFLHPA AATNANSLNS TFSVLPQRFP QFQQHRAVYN SFSFPGQAAR 490 500 510 520 530 540 YPWMAFPRNS IMHLNHTANP TSNSNFLDLN LPPQHNTGLG GIPVAGEEEV KVSTMPLSTS 550 SHSLQQGQQP TSLHTTVA
	Isoform 4 [UniParc]. Checksum: 3D432CA88242C77E	FASTA	642	70,787
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP 310 320 330 340 350 360 SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT 370 380 390 400 410 420 SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE 430 440 450 460 470 480 KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ 490 500 510 520 530 540 QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP 550 560 570 580 590 600 VAGIPASSGN SLDSLQDDNP PHWLKSLQAL TEMDGPSAAP SQTHHSAPFS TQIPLHRASW 610 620 630 640 NPYPPPSNPS SFHSPPPGFQ TAFRPPSKTP TDLLQSSTLD RH
	Isoform 5 [UniParc]. Checksum: 8B120FF952B46883	FASTA	433	48,485
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP 310 320 330 340 350 360 SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT 370 380 390 400 410 420 SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLTE 430 PIERKRLAVL KRR
	Isoform 6 [UniParc]. Checksum: 43A198C595008B1C	FASTA	767	84,747
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP 310 320 330 340 350 360 SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT 370 380 390 400 410 420 SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE 430 440 450 460 470 480 KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ 490 500 510 520 530 540 QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP 550 560 570 580 590 600 VADNSSSIES LNMKEWQDGL RALLPNININ FGGLPNSSSP SNANHSAPTS NTATTDSLSW 610 620 630 640 650 660 DSPGSWTDPA IITGIPASSG NSLDSLQDDN PPHWLKSLQA LTEMDGPSAA PSQTHHSAPF 670 680 690 700 710 720 STQIPLHRAS WNPYPPPSNP SSFHSPPPQI YYRVQHWTAI RQRGATIRKC RICPTLFSPS 730 740 750 760 QPTTHSSLLI SYVLKNPVPD QFFFSLTPDA MRSQGHYHLF INCKNFC
	Isoform 7 [UniParc]. Checksum: FA54396714B4A41B	FASTA	675	74,524
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP 310 320 330 340 350 360 SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT 370 380 390 400 410 420 SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE 430 440 450 460 470 480 KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ 490 500 510 520 530 540 QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP 550 560 570 580 590 600 VAGIPASSGN SLDSLQDDNP PHWLKSLQAL TEMDGQRCSI TDPPQRPLQH TDPAAQSQLE 610 620 630 640 650 660 SLPSSFKPFQ LPLPTPRLSD GLQTPQQNPH RFTTEFNTGP PLGKEEQPLE NAGFVPLCFL 670 PLSPPPTAPF SSLMF
	Isoform 8 [UniParc]. Checksum: C22E9F609FFFF456	FASTA	639	70,339
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ SPIDKPSDSL SIGNGDNSQQ ISNSDTPSPP 310 320 330 340 350 360 PGLSKSNPVI PISSSNHSAR SPFEGAVTES QSLFSDNFRH PNPIPSGLPP FPSSPQTSSD 370 380 390 400 410 420 WPTAPEPQSL FTSETIPVSS STDWQAAFGF GSSKQPEDDL GFDPFDVTRK ALADLIEKEL 430 440 450 460 470 480 SVQDQPSLSP TSLQNSSSHT TTAKGPGSGF LHPAAATNAN SLNSTFSVLP QRFPQFQQHR 490 500 510 520 530 540 AVYNSFSFPG QAARYPWMAF PRNSIMHLNH TANPTSNSNF LDLNLPPQHN TGLGGIPVAG 550 560 570 580 590 600 IPASSGNSLD SLQDDNPPHW LKSLQALTEM DGPSAAPSQT HHSAPFSTQI PLHRASWNPY 610 620 630 PPPSNPSSFH SPPPGFQTAF RPPSKTPTDL LQSSTLDRH
	Isoform 9 [UniParc]. Checksum: 118DE9B8D4B7AD79	FASTA	710	77,809
	10 20 30 40 50 60 MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY 70 80 90 100 110 120 PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL 130 140 150 160 170 180 ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV 190 200 210 220 230 240 VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL 250 260 270 280 290 300 LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ SPIDKPSDSL SIGNGDNSQQ ISNSDTPSPP 310 320 330 340 350 360 PGLSKSNPVI PISSSNHSAR SPFEGAVTES QSLFSDNFRH PNPIPSGLPP FPSSPQTSSD 370 380 390 400 410 420 WPTAPEPQSL FTSETIPVSS STDWQAAFGF GSSKQPEDDL GFDPFDVTRK ALADLIEKEL 430 440 450 460 470 480 SVQDQPSLSP TSLQNSSSHT TTAKGPGSGF LHPAAATNAN SLNSTFSVLP QRFPQFQQHR 490 500 510 520 530 540 AVYNSFSFPG QAARYPWMAF PRNSIMHLNH TANPTSNSNF LDLNLPPQHN TGLGGIPVAD 550 560 570 580 590 600 NSSSVESLNM KEWQDGLRAL LPNININFGG LPNSSSPSNA NHSAPTSNTA TTDSLSWDSP 610 620 630 640 650 660 GSWTDPAIIT GIPASSGNSL DSLQDDNPPH WLKSLQALTE MDGPSAAPSQ THHSAPFSTQ 670 680 690 700 710 IPLHRASWNP YPPPSNPSSF HSPPPGFQTA FRPPSKTPTD LLQSSTLDRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of human and murine homologues of yeast NOT4 gene." Chiang P.-W. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 4).
[2]	"Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits." Albert T.K., Lemaire M., van Berkum N.L., Gentz R., Collart M.A., Timmers H.T.M. Nucleic Acids Res. 28:809-817(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
[3]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT GLY-7.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 9), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-575 (ISOFORM 6). Tissue: Mammary gland and Placenta.
[5]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[7]	"Full-insert sequence of mapped XREF EST." Barrow I.K.-P., Boguski M.S., Touchman J.W., Spencer F. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-575 (ISOFORM 7).
[8]	"Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex." Albert T.K., Hanzawa H., Legtenberg Y.I.A., de Ruwe M.J., van den Heuvel F.A.J., Collart M.A., Boelens R., Timmers H.T.M. EMBO J. 21:355-364(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, UBIQUITINATION, INTERACTION WITH CNOT1 AND UBIQUITIN E2 LIGASES, MUTAGENESIS OF LEU-16; CYS-17; MET-18; CYS-33; TRP-42; ARG-44; ILE-45; GLU-49; PRO-54 AND ARG-57, STRUCTURE BY NMR OF 1-78.
[9]	"An altered-specificity ubiquitin-conjugating enzyme/ubiquitin-protein ligase pair." Winkler G.S., Albert T.K., Dominguez C., Legtenberg Y.I., Boelens R., Timmers H.T. J. Mol. Biol. 337:157-165(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBE2D2, AUTOUBIQUITINATION, MUTAGENESIS OF GLU-49.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324; SER-432 AND SER-490, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Not4 enhances JAK/STAT pathway-dependent gene expression in Drosophila and in human cells." Gronholm J., Kaustio M., Myllymaki H., Kallio J., Saarikettu J., Kronhamn J., Valanne S., Silvennoinen O., Ramet M. FASEB J. 26:1239-1250(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[15]	"The structure of the C4C4 ring finger of human NOT4 reveals features distinct from those of C3HC4 RING fingers." Hanzawa H., de Ruwe M.J., Albert T.K., van Der Vliet P.C., Timmers H.T.M., Boelens R. J. Biol. Chem. 276:10185-10190(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-78 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U71267 mRNA. Translation: AAD00179.1. Frameshift.
U71268 mRNA. Translation: AAD00180.1. Frameshift.
AF180475 mRNA. Translation: AAF29829.1.
AL389980 mRNA. Translation: CAB97536.1.
AK074671 mRNA. Translation: BAC11125.1. Different initiation.
AK300365 mRNA. Translation: BAH13270.1.
AC083871 Genomic DNA. No translation available.
AC074002 Genomic DNA. No translation available.
BC035590 mRNA. Translation: AAH35590.1.
AF091094 mRNA. Translation: AAC72963.1. Sequence problems.

IPI

IPI00002436.
IPI00410681.
IPI00410682.
IPI00410683.
IPI00410684.
IPI00410685.
IPI00410686.
IPI00925697.
IPI01026266.

RefSeq

NP_001008226.1. NM_001008225.2.
NP_001177776.1. NM_001190847.1.
NP_001177777.1. NM_001190848.1.
NP_001177778.1. NM_001190849.1.
NP_001177779.1. NM_001190850.1.
NP_037448.2. NM_013316.3.

UniGene

Hs.490224.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E4U	NMR	-	A	1-78	[»]
1UR6	NMR	-	B	12-63	[»]

ProteinModelPortal

O95628.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95628. 9 interactions.

STRING

9606.ENSP00000354673.

PTM databases

PhosphoSite

O95628.

Proteomic databases

PaxDb

O95628.

PRIDE

O95628.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000315544; ENSP00000326731; ENSG00000080802.
ENST00000356162; ENSP00000348485; ENSG00000080802.
ENST00000361528; ENSP00000354673; ENSG00000080802.
ENST00000414802; ENSP00000416532; ENSG00000080802.
ENST00000423368; ENSP00000406777; ENSG00000080802.
ENST00000428680; ENSP00000399108; ENSG00000080802.
ENST00000451834; ENSP00000388491; ENSG00000080802.

GeneID

4850.

KEGG

hsa:4850.

UCSC

uc003vss.3. human.
uc003vst.3. human.
uc003vsu.2. human.
uc003vsv.2. human.
uc011kpz.2. human.

Organism-specific databases

CTD

4850.

GeneCards

GC07M135046.

H-InvDB

HIX0007112.

HGNC

HGNC:7880. CNOT4.

HPA

HPA005737.

MIM

604911. gene.

neXtProt

NX_O95628.

PharmGKB

PA26675.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5175.

HOVERGEN

HBG051043.

KO

K10643.

Enzyme and pathway databases

Reactome

REACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

UniPathway

UPA00143.

Gene expression databases

ArrayExpress

O95628.

Bgee

O95628.

CleanEx

HS_CNOT4.

Genevestigator

O95628.

GermOnline

ENSG00000080802. Homo sapiens.

Family and domain databases

Gene3D

3.30.40.10. 1 hit.
3.30.70.330. 1 hit.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR000571. Znf_CCCH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

SMART

SM00184. RING. 1 hit.
SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 1 hit.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O95628.

GenomeRNAi

4850.

NextBio

18678.

SOURCE

Search...

Entry information

Entry name

CNOT4_HUMAN

Accession

Primary (citable) accession number: O95628
Secondary accession number(s): B7Z6I4 , E7ET38, O95339, O95627, Q8IYM7, Q8NCL0, Q9NPQ1, Q9NZN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 3, 2006
Last modified:	May 29, 2013
This is version 123 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.