O95620 (DUS4L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like
EC=1.3.1.-

Alternative name(s):
pp35
tRNA-dihydrouridine synthase 4-like

Gene names

Name:

DUS4L

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family. Dus4 subfamily.
Sequence caution	The sequence AAD00100.1 differs from that shown. Reason: Frameshift at position 14.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA dihydrouridine synthesis Inferred from electronic annotation. Source: GOC
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95620-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95620-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like		PRO_0000247347
Natural variations
	Alternative sequence	1 – 121	121	Missing in isoform 2.		VSP_019975
	Natural variant	178	1	T → A. Corresponds to variant rs6956789 [ dbSNP | Ensembl ].		VAR_048938
	Natural variant	230	1	R → Q. Corresponds to variant rs6957510 [ dbSNP | Ensembl ].		VAR_027094
Experimental info
	Sequence conflict	162	1	H → N in AAD00100. Ref.1
	Sequence conflict	210	1	S → C in AAD00100. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 52A8AF158275B3BF	FASTA	317	35,816
	10 20 30 40 50 60 MKSDCMQTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS CDLCYTPMIV 70 80 90 100 110 120 AADFVKSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA RIVCPYANGI DINCGCPQRW 130 140 150 160 170 180 AMAEGYGACL INKPELVQDM VKQVRNQVET PGFSVSIKIR IHDDLKRTVD LCQKAEATGV 190 200 210 220 230 240 SWITVHGRTA EERHQPVHYD SIKIIKENMS IPVIANGDIR SLKEAENVWR ITGTDGVMVA 250 260 270 280 290 300 RGLLANPAMF AGYEETPLKC IWDWVDIALE LGTPYMCFHQ HLMYMMEKIT SRQEKRVFNA 310 LSSTSAIIDY LTDHYGI
	Isoform 2 [UniParc]. Checksum: F5E4F1C5BF8B050A	FASTA	196	22,227
	10 20 30 40 50 60 MAEGYGACLI NKPELVQDMV KQVRNQVETP GFSVSIKIRI HDDLKRTVDL CQKAEATGVS 70 80 90 100 110 120 WITVHGRTAE ERHQPVHYDS IKIIKENMSI PVIANGDIRS LKEAENVWRI TGTDGVMVAR 130 140 150 160 170 180 GLLANPAMFA GYEETPLKCI WDWVDIALEL GTPYMCFHQH LMYMMEKITS RQEKRVFNAL 190 SSTSAIIDYL TDHYGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene encoding a human placental cDNA homologous with the E.coli yhdg and R. capsulatus nifR3 genes is located on chromosome 7." Laustsen P.G., Kristensen T. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Placenta.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62767 mRNA. Translation: AAD00100.1. Frameshift. AK297195 mRNA. Translation: BAG59682.1. CH471070 Genomic DNA. Translation: EAW83400.1. BC111774 mRNA. Translation: AAI11775.1.
IPI	IPI00021410. IPI00783809.
RefSeq	NP_001257348.1. NM_001270419.1. NP_853559.1. NM_181581.2.
UniGene	Hs.97627.
3D structure databases
ProteinModelPortal	O95620.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000265720.
Proteomic databases
PaxDb	O95620.
PRIDE	O95620.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265720; ENSP00000265720; ENSG00000105865. ENST00000402620; ENSP00000385274; ENSG00000105865.
GeneID	11062.
KEGG	hsa:11062.
UCSC	uc003veh.3. human.
Organism-specific databases
CTD	11062.
GeneCards	GC07P107203.
HGNC	HGNC:21517. DUS4L.
HPA	HPA044301.
neXtProt	NX_O95620.
PharmGKB	PA142671939.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0042.
HOGENOM	HOG000203649.
HOVERGEN	HBG053313.
InParanoid	O95620.
KO	K05545.
OMA	AGPMVRY.
OrthoDB	EOG4ZCT4T.
PhylomeDB	O95620.
Gene expression databases
ArrayExpress	O95620.
Bgee	O95620.
CleanEx	HS_DUS4L.
Genevestigator	O95620.
GermOnline	ENSG00000105865. Homo sapiens.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
PANTHER	PTHR11082. PTHR11082. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	11062.
NextBio	42037.

Entry information

Entry name

DUS4L_HUMAN

Accession

Primary (citable) accession number: O95620
Secondary accession number(s): B4DLX0, Q2NKK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.