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Protein

tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like

Gene

DUS4L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87FMNBy similarity1
Active sitei116Proton donorBy similarity1
Binding sitei158FMNBy similarity1
Binding sitei186FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 35FMNBy similarity3
Nucleotide bindingi216 – 218FMNBy similarity3
Nucleotide bindingi240 – 241FMNBy similarity2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciZFISH:ENSG00000105865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
pp35
tRNA-dihydrouridine synthase 4-like
Gene namesi
Name:DUS4L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:21517. DUS4L.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi11062.
OpenTargetsiENSG00000105865.
PharmGKBiPA142671939.

Polymorphism and mutation databases

BioMutaiDUS4L.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002473471 – 317tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-likeAdd BLAST317

Proteomic databases

EPDiO95620.
MaxQBiO95620.
PaxDbiO95620.
PeptideAtlasiO95620.
PRIDEiO95620.

PTM databases

iPTMnetiO95620.
PhosphoSitePlusiO95620.

Expressioni

Gene expression databases

BgeeiENSG00000105865.
CleanExiHS_DUS4L.
ExpressionAtlasiO95620. baseline and differential.
GenevisibleiO95620. HS.

Organism-specific databases

HPAiHPA044301.

Interactioni

Protein-protein interaction databases

BioGridi116246. 6 interactors.
STRINGi9606.ENSP00000265720.

Structurei

3D structure databases

ProteinModelPortaliO95620.
SMRiO95620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus4 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2335. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000074907.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiO95620.
KOiK05545.
OMAiRPHHDIA.
OrthoDBiEOG091G0EQJ.
PhylomeDBiO95620.
TreeFamiTF105618.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95620-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSDCMQTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS
60 70 80 90 100
CDLCYTPMIV AADFVKSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA
110 120 130 140 150
RIVCPYANGI DINCGCPQRW AMAEGYGACL INKPELVQDM VKQVRNQVET
160 170 180 190 200
PGFSVSIKIR IHDDLKRTVD LCQKAEATGV SWITVHGRTA EERHQPVHYD
210 220 230 240 250
SIKIIKENMS IPVIANGDIR SLKEAENVWR ITGTDGVMVA RGLLANPAMF
260 270 280 290 300
AGYEETPLKC IWDWVDIALE LGTPYMCFHQ HLMYMMEKIT SRQEKRVFNA
310
LSSTSAIIDY LTDHYGI
Length:317
Mass (Da):35,816
Last modified:July 25, 2006 - v2
Checksum:i52A8AF158275B3BF
GO
Isoform 2 (identifier: O95620-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:196
Mass (Da):22,227
Checksum:iF5E4F1C5BF8B050A
GO

Sequence cautioni

The sequence AAD00100 differs from that shown. Reason: Frameshift at position 14.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162H → N in AAD00100 (Ref. 1) Curated1
Sequence conflicti210S → C in AAD00100 (Ref. 1) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048938178T → A.Corresponds to variant rs6956789dbSNPEnsembl.1
Natural variantiVAR_027094230R → Q.Corresponds to variant rs6957510dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0199751 – 121Missing in isoform 2. 2 PublicationsAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62767 mRNA. Translation: AAD00100.1. Frameshift.
AK297195 mRNA. Translation: BAG59682.1.
CH471070 Genomic DNA. Translation: EAW83400.1.
BC111774 mRNA. Translation: AAI11775.1.
CCDSiCCDS5745.1. [O95620-1]
RefSeqiNP_001257348.1. NM_001270419.1. [O95620-1]
NP_853559.1. NM_181581.2. [O95620-1]
XP_005250182.1. XM_005250125.4. [O95620-1]
XP_011514068.1. XM_011515766.2. [O95620-1]
XP_011514069.1. XM_011515767.2. [O95620-1]
XP_016867197.1. XM_017011708.1. [O95620-1]
UniGeneiHs.97627.

Genome annotation databases

EnsembliENST00000265720; ENSP00000265720; ENSG00000105865. [O95620-1]
GeneIDi11062.
KEGGihsa:11062.
UCSCiuc003veh.5. human. [O95620-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62767 mRNA. Translation: AAD00100.1. Frameshift.
AK297195 mRNA. Translation: BAG59682.1.
CH471070 Genomic DNA. Translation: EAW83400.1.
BC111774 mRNA. Translation: AAI11775.1.
CCDSiCCDS5745.1. [O95620-1]
RefSeqiNP_001257348.1. NM_001270419.1. [O95620-1]
NP_853559.1. NM_181581.2. [O95620-1]
XP_005250182.1. XM_005250125.4. [O95620-1]
XP_011514068.1. XM_011515766.2. [O95620-1]
XP_011514069.1. XM_011515767.2. [O95620-1]
XP_016867197.1. XM_017011708.1. [O95620-1]
UniGeneiHs.97627.

3D structure databases

ProteinModelPortaliO95620.
SMRiO95620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116246. 6 interactors.
STRINGi9606.ENSP00000265720.

PTM databases

iPTMnetiO95620.
PhosphoSitePlusiO95620.

Polymorphism and mutation databases

BioMutaiDUS4L.

Proteomic databases

EPDiO95620.
MaxQBiO95620.
PaxDbiO95620.
PeptideAtlasiO95620.
PRIDEiO95620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265720; ENSP00000265720; ENSG00000105865. [O95620-1]
GeneIDi11062.
KEGGihsa:11062.
UCSCiuc003veh.5. human. [O95620-1]

Organism-specific databases

CTDi11062.
DisGeNETi11062.
GeneCardsiDUS4L.
HGNCiHGNC:21517. DUS4L.
HPAiHPA044301.
neXtProtiNX_O95620.
OpenTargetsiENSG00000105865.
PharmGKBiPA142671939.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2335. Eukaryota.
COG0042. LUCA.
GeneTreeiENSGT00550000074907.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiO95620.
KOiK05545.
OMAiRPHHDIA.
OrthoDBiEOG091G0EQJ.
PhylomeDBiO95620.
TreeFamiTF105618.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000105865-MONOMER.

Miscellaneous databases

GenomeRNAii11062.
PROiO95620.

Gene expression databases

BgeeiENSG00000105865.
CleanExiHS_DUS4L.
ExpressionAtlasiO95620. baseline and differential.
GenevisibleiO95620. HS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS4L_HUMAN
AccessioniPrimary (citable) accession number: O95620
Secondary accession number(s): B4DLX0, Q2NKK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: November 2, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.