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Protein

tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like

Gene

DUS4L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.By similarity

Cofactori

FADBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. tRNA dihydrouridine synthase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like (EC:1.3.1.-)
Alternative name(s):
pp35
tRNA-dihydrouridine synthase 4-like
Gene namesi
Name:DUS4L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:21517. DUS4L.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671939.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-likePRO_0000247347Add
BLAST

Proteomic databases

MaxQBiO95620.
PaxDbiO95620.
PRIDEiO95620.

Expressioni

Gene expression databases

BgeeiO95620.
CleanExiHS_DUS4L.
ExpressionAtlasiO95620. baseline and differential.
GenevestigatoriO95620.

Organism-specific databases

HPAiHPA044301.

Interactioni

Protein-protein interaction databases

BioGridi116246. 1 interaction.
STRINGi9606.ENSP00000265720.

Structurei

3D structure databases

ProteinModelPortaliO95620.
SMRiO95620. Positions 32-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. Dus4 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0042.
GeneTreeiENSGT00550000074907.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiO95620.
KOiK05545.
OMAiHHHLIYM.
OrthoDBiEOG751NG3.
PhylomeDBiO95620.
TreeFamiTF105618.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O95620-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSDCMQTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS
60 70 80 90 100
CDLCYTPMIV AADFVKSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA
110 120 130 140 150
RIVCPYANGI DINCGCPQRW AMAEGYGACL INKPELVQDM VKQVRNQVET
160 170 180 190 200
PGFSVSIKIR IHDDLKRTVD LCQKAEATGV SWITVHGRTA EERHQPVHYD
210 220 230 240 250
SIKIIKENMS IPVIANGDIR SLKEAENVWR ITGTDGVMVA RGLLANPAMF
260 270 280 290 300
AGYEETPLKC IWDWVDIALE LGTPYMCFHQ HLMYMMEKIT SRQEKRVFNA
310
LSSTSAIIDY LTDHYGI
Length:317
Mass (Da):35,816
Last modified:July 25, 2006 - v2
Checksum:i52A8AF158275B3BF
GO
Isoform 2 (identifier: O95620-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:196
Mass (Da):22,227
Checksum:iF5E4F1C5BF8B050A
GO

Sequence cautioni

The sequence AAD00100.1 differs from that shown. Reason: Frameshift at position 14. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621H → N in AAD00100. 1 PublicationCurated
Sequence conflicti210 – 2101S → C in AAD00100. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti178 – 1781T → A.
Corresponds to variant rs6956789 [ dbSNP | Ensembl ].
VAR_048938
Natural varianti230 – 2301R → Q.
Corresponds to variant rs6957510 [ dbSNP | Ensembl ].
VAR_027094

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 121121Missing in isoform 2. 2 PublicationsVSP_019975Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62767 mRNA. Translation: AAD00100.1. Frameshift.
AK297195 mRNA. Translation: BAG59682.1.
CH471070 Genomic DNA. Translation: EAW83400.1.
BC111774 mRNA. Translation: AAI11775.1.
CCDSiCCDS5745.1. [O95620-1]
RefSeqiNP_001257348.1. NM_001270419.1. [O95620-1]
NP_853559.1. NM_181581.2. [O95620-1]
XP_005250182.1. XM_005250125.2. [O95620-1]
UniGeneiHs.97627.

Genome annotation databases

EnsembliENST00000265720; ENSP00000265720; ENSG00000105865. [O95620-1]
GeneIDi11062.
KEGGihsa:11062.
UCSCiuc003veh.4. human. [O95620-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62767 mRNA. Translation: AAD00100.1. Frameshift.
AK297195 mRNA. Translation: BAG59682.1.
CH471070 Genomic DNA. Translation: EAW83400.1.
BC111774 mRNA. Translation: AAI11775.1.
CCDSiCCDS5745.1. [O95620-1]
RefSeqiNP_001257348.1. NM_001270419.1. [O95620-1]
NP_853559.1. NM_181581.2. [O95620-1]
XP_005250182.1. XM_005250125.2. [O95620-1]
UniGeneiHs.97627.

3D structure databases

ProteinModelPortaliO95620.
SMRiO95620. Positions 32-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116246. 1 interaction.
STRINGi9606.ENSP00000265720.

Proteomic databases

MaxQBiO95620.
PaxDbiO95620.
PRIDEiO95620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000265720; ENSP00000265720; ENSG00000105865. [O95620-1]
GeneIDi11062.
KEGGihsa:11062.
UCSCiuc003veh.4. human. [O95620-1]

Organism-specific databases

CTDi11062.
GeneCardsiGC07P107203.
HGNCiHGNC:21517. DUS4L.
HPAiHPA044301.
neXtProtiNX_O95620.
PharmGKBiPA142671939.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0042.
GeneTreeiENSGT00550000074907.
HOGENOMiHOG000203649.
HOVERGENiHBG053313.
InParanoidiO95620.
KOiK05545.
OMAiHHHLIYM.
OrthoDBiEOG751NG3.
PhylomeDBiO95620.
TreeFamiTF105618.

Miscellaneous databases

GenomeRNAii11062.
NextBioi42037.
PROiO95620.

Gene expression databases

BgeeiO95620.
CleanExiHS_DUS4L.
ExpressionAtlasiO95620. baseline and differential.
GenevestigatoriO95620.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene encoding a human placental cDNA homologous with the E.coli yhdg and R. capsulatus nifR3 genes is located on chromosome 7."
    Laustsen P.G., Kristensen T.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Placenta.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Entry informationi

Entry nameiDUS4L_HUMAN
AccessioniPrimary (citable) accession number: O95620
Secondary accession number(s): B4DLX0, Q2NKK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: January 7, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.