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O95620 (DUS4L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like

EC=1.3.1.-
Alternative name(s):
pp35
tRNA-dihydrouridine synthase 4-like
Gene names
Name:DUS4L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.

Cofactor

FAD By similarity.

Sequence similarities

Belongs to the Dus family. Dus4 subfamily.

Sequence caution

The sequence AAD00100.1 differs from that shown. Reason: Frameshift at position 14.

Ontologies

Keywords
   Biological processtRNA processing
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

tRNA dihydrouridine synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95620-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95620-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like
PRO_0000247347

Natural variations

Alternative sequence1 – 121121Missing in isoform 2.
VSP_019975
Natural variant1781T → A.
Corresponds to variant rs6956789 [ dbSNP | Ensembl ].
VAR_048938
Natural variant2301R → Q.
Corresponds to variant rs6957510 [ dbSNP | Ensembl ].
VAR_027094

Experimental info

Sequence conflict1621H → N in AAD00100. Ref.1
Sequence conflict2101S → C in AAD00100. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: 52A8AF158275B3BF

FASTA31735,816
        10         20         30         40         50         60 
MKSDCMQTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS CDLCYTPMIV 

        70         80         90        100        110        120 
AADFVKSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA RIVCPYANGI DINCGCPQRW 

       130        140        150        160        170        180 
AMAEGYGACL INKPELVQDM VKQVRNQVET PGFSVSIKIR IHDDLKRTVD LCQKAEATGV 

       190        200        210        220        230        240 
SWITVHGRTA EERHQPVHYD SIKIIKENMS IPVIANGDIR SLKEAENVWR ITGTDGVMVA 

       250        260        270        280        290        300 
RGLLANPAMF AGYEETPLKC IWDWVDIALE LGTPYMCFHQ HLMYMMEKIT SRQEKRVFNA 

       310 
LSSTSAIIDY LTDHYGI 

« Hide

Isoform 2 [UniParc].

Checksum: F5E4F1C5BF8B050A
Show »

FASTA19622,227

References

« Hide 'large scale' references
[1]"The gene encoding a human placental cDNA homologous with the E.coli yhdg and R. capsulatus nifR3 genes is located on chromosome 7."
Laustsen P.G., Kristensen T.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Placenta.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U62767 mRNA. Translation: AAD00100.1. Frameshift.
AK297195 mRNA. Translation: BAG59682.1.
CH471070 Genomic DNA. Translation: EAW83400.1.
BC111774 mRNA. Translation: AAI11775.1.
CCDSCCDS5745.1. [O95620-1]
RefSeqNP_001257348.1. NM_001270419.1. [O95620-1]
NP_853559.1. NM_181581.2. [O95620-1]
XP_005250182.1. XM_005250125.2. [O95620-1]
UniGeneHs.97627.

3D structure databases

ProteinModelPortalO95620.
SMRO95620. Positions 32-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116246. 1 interaction.
STRING9606.ENSP00000265720.

Proteomic databases

MaxQBO95620.
PaxDbO95620.
PRIDEO95620.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265720; ENSP00000265720; ENSG00000105865. [O95620-1]
ENST00000402620; ENSP00000385274; ENSG00000105865. [O95620-2]
GeneID11062.
KEGGhsa:11062.
UCSCuc003veh.4. human. [O95620-1]

Organism-specific databases

CTD11062.
GeneCardsGC07P107203.
HGNCHGNC:21517. DUS4L.
HPAHPA044301.
neXtProtNX_O95620.
PharmGKBPA142671939.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0042.
HOGENOMHOG000203649.
HOVERGENHBG053313.
InParanoidO95620.
KOK05545.
OMAIVYTPMI.
OrthoDBEOG751NG3.
PhylomeDBO95620.
TreeFamTF105618.

Gene expression databases

ArrayExpressO95620.
BgeeO95620.
CleanExHS_DUS4L.
GenevestigatorO95620.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERPTHR11082. PTHR11082. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PIRSFPIRSF006621. Dus. 1 hit.
PROSITEPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi11062.
NextBio42037.
PROO95620.

Entry information

Entry nameDUS4L_HUMAN
AccessionPrimary (citable) accession number: O95620
Secondary accession number(s): B4DLX0, Q2NKK1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM