O95613 (PCNT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pericentrin

Alternative name(s):
Kendrin
Pericentrin-B

Gene names

Name:	PCNT
Synonyms:	KIAA0402, PCNT2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	3336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. Ref.1 Ref.8 Ref.9 Ref.13
Subunit structure	Interacts with CHD3. Interacts with CHD4; the interaction regulates centrosome integrity By similarity. Interacts with DISC1 and PCM1. Binds calmodulin. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of PCNT and CDK5RAP2. Interacts with isoform 1 of NEK2. Ref.1 Ref.9 Ref.13 Ref.14
Subcellular location	Cytoplasm › cytoskeleton › centrosome. Note: Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. Ref.1 Ref.8 Ref.9
Tissue specificity	Expressed in all tissues tested, including placenta, liver, kidney and thymus. Ref.8
Domain	Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. Ref.8
Involvement in disease	Microcephalic osteodysplastic primordial dwarfism 2 (MOPD2) [MIM:210720]: Adults with this rare inherited condition have an average height of 100 centimeters and a brain size comparable to that of a 3-month-old baby, but are of near-normal intelligence. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.11
Sequence caution	The sequence AAD10838.1 differs from that shown. Reason: Frameshift at position 3320. The sequence BAA23698.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAC04252.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Dwarfism
Domain	Coiled coil
Ligand	Calmodulin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Traceable author statement. Source: Reactome apoptotic process Inferred from electronic annotation. Source: Compara brain morphogenesis Inferred from electronic annotation. Source: Compara cerebellar cortex morphogenesis Inferred from electronic annotation. Source: Compara cilium assembly Inferred from direct assay PubMed 15337773. Source: MGI in utero embryonic development Inferred from electronic annotation. Source: Compara limb morphogenesis Inferred from electronic annotation. Source: Compara microtubule cytoskeleton organization Inferred from mutant phenotype Ref.9. Source: UniProtKB multicellular organism growth Inferred from electronic annotation. Source: Compara neural precursor cell proliferation Inferred from electronic annotation. Source: Compara neuron migration Inferred from electronic annotation. Source: Compara olfactory bulb development Inferred from electronic annotation. Source: Compara spindle organization Inferred from electronic annotation. Source: Compara
Cellular_component	centriole Inferred from electronic annotation. Source: Compara centrosome Inferred from direct assay Ref.9PubMed 19543530PubMed 21399614. Source: UniProtKB cytosol Traceable author statement. Source: Reactome intercellular bridge Inferred from electronic annotation. Source: Compara microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DISC1	Q9NRI5	5	EBI-530012,EBI-529989

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95613-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95613-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.
     2839-2917: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 3336	3336	Pericentrin		PRO_0000058257
Regions
	Region	2983 – 3246	264	Interaction with NEK2
	Region	3195 – 3208	14	Calmodulin-binding
	Coiled coil	258 – 553	296	Potential
	Coiled coil	675 – 835	161	Potential
	Coiled coil	1010 – 1146	137	Potential
	Coiled coil	1299 – 1949	651	Potential
	Coiled coil	2064 – 2082	19	Potential
	Coiled coil	2536 – 3086	551	Potential
	Compositional bias	214 – 795	582	Glu-rich
Amino acid modifications
	Modified residue	191	1	Phosphothreonine Ref.15
	Modified residue	366	1	Phosphoserine By similarity
	Modified residue	682	1	Phosphoserine Ref.15
	Modified residue	1712	1	Phosphoserine By similarity
	Modified residue	2044	1	Phosphoserine Ref.15
	Modified residue	2177	1	Phosphoserine Ref.12
	Modified residue	2327	1	Phosphoserine Ref.12
	Modified residue	2477	1	Phosphoserine Ref.15
	Modified residue	2486	1	Phosphoserine Ref.15
	Modified residue	3302	1	Phosphoserine Ref.15
Natural variations
	Alternative sequence	1 – 118	118	Missing in isoform 2.		VSP_040104
	Alternative sequence	2839 – 2917	79	Missing in isoform 2.		VSP_040105
	Natural variant	539	1	T → I. Corresponds to variant rs2249060 [ dbSNP | Ensembl ].		VAR_043878
	Natural variant	704	1	G → E. Ref.1 Ref.2 Ref.3 Corresponds to variant rs2839223 [ dbSNP | Ensembl ].		VAR_043879
	Natural variant	879	1	T → A. Ref.1 Corresponds to variant rs2839227 [ dbSNP | Ensembl ].		VAR_043880
	Natural variant	1038	1	V → A. Ref.1 Ref.3 Ref.7 Corresponds to variant rs6518289 [ dbSNP | Ensembl ].		VAR_043881
	Natural variant	1163	1	R → C. Corresponds to variant rs7279204 [ dbSNP | Ensembl ].		VAR_043882
	Natural variant	1194	1	A → T. Corresponds to variant rs35044802 [ dbSNP | Ensembl ].		VAR_043883
	Natural variant	1639	1	I → V. Corresponds to variant rs6518291 [ dbSNP | Ensembl ].		VAR_043884
	Natural variant	1841	1	N → S. Corresponds to variant rs35940413 [ dbSNP | Ensembl ].		VAR_043885
	Natural variant	1953	1	R → H. Corresponds to variant rs34268261 [ dbSNP | Ensembl ].		VAR_043886
	Natural variant	1960	1	R → Q. Corresponds to variant rs34813667 [ dbSNP | Ensembl ].		VAR_043887
	Natural variant	2097	1	L → P. Corresponds to variant rs2839245 [ dbSNP | Ensembl ].		VAR_043888
	Natural variant	2125	1	H → P. Corresponds to variant rs35978208 [ dbSNP | Ensembl ].		VAR_043889
	Natural variant	2188	1	M → R. Ref.1 Corresponds to variant rs1044998 [ dbSNP | Ensembl ].		VAR_043890
	Natural variant	2191	1	S → P. Corresponds to variant rs34151633 [ dbSNP | Ensembl ].		VAR_043891
	Natural variant	2239	1	W → R. Corresponds to variant rs35346764 [ dbSNP | Ensembl ].		VAR_056961
	Natural variant	2274	1	P → L. Corresponds to variant rs2070425 [ dbSNP | Ensembl ].		VAR_056962
	Natural variant	2329	1	P → R. Corresponds to variant rs35848602 [ dbSNP | Ensembl ].		VAR_056963
	Natural variant	2361	1	Q → R. Corresponds to variant rs7277175 [ dbSNP | Ensembl ].		VAR_056964
	Natural variant	2549	1	A → T. Ref.1 Corresponds to variant rs2839256 [ dbSNP | Ensembl ].		VAR_056965
	Natural variant	2625	1	R → Q. Corresponds to variant rs8131693 [ dbSNP | Ensembl ].		VAR_056966
	Natural variant	2659	1	Q → H. Corresponds to variant rs2070426 [ dbSNP | Ensembl ].		VAR_056967
	Natural variant	2753	1	R → H. Corresponds to variant rs743346 [ dbSNP | Ensembl ].		VAR_056968
	Natural variant	2792	1	Q → R. Corresponds to variant rs2073376 [ dbSNP | Ensembl ].		VAR_056969
	Natural variant	2903	1	A → T. Corresponds to variant rs35147998 [ dbSNP | Ensembl ].		VAR_056970
	Natural variant	2975	1	L → P. Corresponds to variant rs35881595 [ dbSNP | Ensembl ].		VAR_056971
	Natural variant	3091	1	S → G. Corresponds to variant rs4818842 [ dbSNP | Ensembl ].		VAR_056972
	Natural variant	3245	1	R → S. Corresponds to variant rs2073380 [ dbSNP | Ensembl ].		VAR_056973
Experimental info
	Mutagenesis	3196 – 3197	2	FR → AA: Decrease in calmodulin binding.
	Mutagenesis	3203	1	V → A: Decrease in calmodulin binding. Ref.8
	Mutagenesis	3208 – 3209	2	RL → AA: Decrease in calmodulin binding.
	Sequence conflict	703	1	Y → F in AAD10838. Ref.1
	Sequence conflict	789	1	F → L in AAD10838. Ref.1
	Sequence conflict	819	1	G → A in AAD10838. Ref.1
	Sequence conflict	890 – 900	11	Missing in AAD10838. Ref.1
	Sequence conflict	967	1	S → T in AAD10838. Ref.1
	Sequence conflict	1024	1	E → K in AAD10838. Ref.1
	Sequence conflict	1036	1	T → A in BAC04252. Ref.7
	Sequence conflict	1287	1	I → L in AAD10838. Ref.1
	Sequence conflict	1317	1	K → T in AAD10838. Ref.1
	Sequence conflict	1534	1	Q → H in AAD10838. Ref.1
	Sequence conflict	3136	1	Y → S in AAD10838. Ref.1
	Sequence conflict	3300	1	E → G in AAD10838. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 4. Checksum: 4F182D2C201662A8	FASTA	3,336	378,037
	10 20 30 40 50 60 MEVEQEQRRR KVEAGRTKLA HFRQRKTKGD SSHSEKKTAK RKGSAVDASV QEESPVTKED 70 80 90 100 110 120 SALCGGGDIC KSTSCDDTPD GAGGAFAAQP EDCDGEKRED LEQLQQKQVN DHPPEQCGMF 130 140 150 160 170 180 TVSDHPPEQH GMFTVGDHPP EQRGMFTVSD HPPEQHGMFT VSDHPPEQRG MFTISDHQPE 190 200 210 220 230 240 QRGMFTVSDH TPEQRGIFTI SDHPAEQRGM FTKECEQECE LAITDLESGR EDEAGLHQSQ 250 260 270 280 290 300 AVHGLELEAL RLSLSNMHTA QLELTQANLQ KEKETALTEL REMLNSRRAQ ELALLQSRQQ 310 320 330 340 350 360 HELELLREQH AREKEEVVLR CGQEAAELKE KLQSEMEKNA QIVKTLKEDW ESEKDLCLEN 370 380 390 400 410 420 LRKELSAKHQ SEMEDLQNQF QKELAEQRAE LEKIFQDKNQ AERALRNLES HHQAAIEKLR 430 440 450 460 470 480 EDLQSEHGRC LEDLEFKFKE SEKEKQLELE NLQASYEDLK AQSQEEIRRL WSQLDSARTS 490 500 510 520 530 540 RQELSELHEQ LLARTSRVED LEQLKQREKT QHESELEQLR IYFEKKLRDA EKTYQEDLTL 550 560 570 580 590 600 LQQRLQGARE DALLDSVEVG LSCVGLEEKP EKGRKDHVDE LEPERHKESL PRFQAELEES 610 620 630 640 650 660 HRHQLEALES PLCIQHEGHV SDRCCVETSA LGHEWRLEPS EGHSQELPWV HLQGVQDGDL 670 680 690 700 710 720 EADTERAARV LGLETEHKVQ LSLLQTELKE EIELLKIENR NLYGKLQHET RLKDDLEKVK 730 740 750 760 770 780 HNLIEDHQKE LNNAKQKTEL MKQEFQRKET DWKVMKEELQ REAEEKLTLM LLELREKAES 790 800 810 820 830 840 EKQTIINKFE LREAEMRQLQ DQQAAQILDL ERSLTEQQGR LQQLEQDLTS DDALHCSQCG 850 860 870 880 890 900 REPPTAQDGE LAALHVKEDC ALQLMLARSR FLEERKEITE KFSAEQDAFL QEAQEQHARE 910 920 930 940 950 960 LQLLQERHQQ QLLSVTAELE ARHQAALGEL TASLESKQGA LLAARVAELQ TKHAADLGAL 970 980 990 1000 1010 1020 ETRHLSSLDS LESCYLSEFQ TIREEHRQAL ELLRADFEEQ LWKKDSLHQT ILTQELEKLK 1030 1040 1050 1060 1070 1080 RKHEGELQSV RDHLRTEVST ELAGTVAHEL QGVHQGEFGS EKKTALHEKE ETLRLQSAQA 1090 1100 1110 1120 1130 1140 QPFHQEEKES LSLQLQKKNH QVQQLKDQVL SLSHEIEECR SELEVLQQRR ERENREGANL 1150 1160 1170 1180 1190 1200 LSMLKADVNL SHSERGALQD ALRRLLGLFG ETLRAAVTLR SRIGERVGLC LDDAGAGLAL 1210 1220 1230 1240 1250 1260 STAPALEETW SDVALPELDR TLSECAEMSS VAEISSHMRE SFLMSPESVR ECEQPIRRVF 1270 1280 1290 1300 1310 1320 QSLSLAVDGL MEMALDSSRQ LEEARQIHSR FEKEFSFKNE ETAQVVRKHQ ELLECLKEES 1330 1340 1350 1360 1370 1380 AAKAELALEL HKTQGTLEGF KVETADLKEV LAGKEDSEHR LVLELESLRR QLQQAAQEQA 1390 1400 1410 1420 1430 1440 ALREECTRLW SRGEATATDA EAREAALRKE VEDLTKEQSE TRKQAEKDRS ALLSQMKILE 1450 1460 1470 1480 1490 1500 SELEEQLSQH RGCAKQAEAV TALEQQVASL DKHLRNQRQF MDEQAAEREH EREEFQQEIQ 1510 1520 1530 1540 1550 1560 RLEGQLRQAA KPQPWGPRDS QQAPLDGEVE LLQQKLREKL DEFNELAIQK ESADRQVLMQ 1570 1580 1590 1600 1610 1620 EEEIKRLEEM NINIRKKVAQ LQEEVEKQKN IVKGLEQDKE VLKKQQMSSL LLASTLQSTL 1630 1640 1650 1660 1670 1680 DAGRCPEPPS GSPPEGPEIQ LEVTQRALLR RESEVLDLKE QLEKMKGDLE SKNEEILHLN 1690 1700 1710 1720 1730 1740 LKLDMQNSQT AVSLRELEEE NTSLKVIYTR SSEIEELKAT IENLQENQKR LQKEKAEEIE 1750 1760 1770 1780 1790 1800 QLHEVIEKLQ HELSLMGPVV HEVSDSQAGS LQSELLCSQA GGPRGQALQG ELEAALEAKE 1810 1820 1830 1840 1850 1860 ALSRLLADQE RRHSQALEAL QQRLQGAEEA AELQLAELER NVALREAEVE DMASRIQEFE 1870 1880 1890 1900 1910 1920 AALKAKEATI AERNLEIDAL NQRKAAHSAE LEAVLLALAR IRRALEQQPL AAGAAPPELQ 1930 1940 1950 1960 1970 1980 WLRAQCARLS RQLQVLHQRF LRCQVELDRR QARRATAHTR VPGAHPQPRM DGGAKAQVTG 1990 2000 2010 2020 2030 2040 DVEASHDAAL EPVVPDPQGD LQPVLVTLKD APLCKQEGVM SVLTVCQRQL QSELLLVKNE 2050 2060 2070 2080 2090 2100 MRLSLEDGGK GKEKVLEDCQ LPKVDLVAQV KQLQEKLNRL LYSMTFQNVD AADTKSLWPM 2110 2120 2130 2140 2150 2160 ASAHLLESSW SDDSCDGEEP DISPHIDTCD ANTATGGVTD VIKNQAIDAC DANTTPGGVT 2170 2180 2190 2200 2210 2220 DVIKNWDSLI PDEMPDSPIQ EKSECQDMSL SSPTSVLGGS RHQSHTAEAG PRKSPVGMLD 2230 2240 2250 2260 2270 2280 LSSWSSPEVL RKDWTLEPWP SLPVTPHSGA LSLCSADTSL GDRADTSLPQ TQGPGLLCSP 2290 2300 2310 2320 2330 2340 GVSAAALALQ WAESPPADDH HVQRTAVEKD VEDFITTSFD SQETLSSPPP GLEGKADRSE 2350 2360 2370 2380 2390 2400 KSDGSGFGAR LSPGSGGPEA QTAGPVTPAS ISGRFQPLPE AMKEKEVRPK HVKALLQMVR 2410 2420 2430 2440 2450 2460 DESHQILALS EGLAPPSGEP HPPRKEDEIQ DISLHGGKTQ EVPTACPDWR GDLLQVVQEA 2470 2480 2490 2500 2510 2520 FEKEQEMQGV ELQPRLSGSD LGGHSSLLER LEKIIREQGD LQEKSLEHLR LPDRSSLLSE 2530 2540 2550 2560 2570 2580 IQALRAQLRM THLQNQEKLQ HLRTALTSAE ARGSQQEHQL RRQVELLAYK VEQEKCIAGD 2590 2600 2610 2620 2630 2640 LQKTLSEEQE KANSVQKLLA AEQTVVRDLK SDLCESRQKS EQLSRSLCEV QQEVLQLRSM 2650 2660 2670 2680 2690 2700 LSSKENELKA ALQELESEQG KGRALQSQLE EEQLRHLQRE SQSAKALEEL RASLETQRAQ 2710 2720 2730 2740 2750 2760 SSRLCVALKH EQTAKDNLQK ELRIEHSRCE ALLAQERSQL SELQKDLAAE KSRTLELSEA 2770 2780 2790 2800 2810 2820 LRHERLLTEQ LSQRTQEACV HQDTQAHHAL LQKLKEEKSR VVDLQAMLEK VQQQALHSQQ 2830 2840 2850 2860 2870 2880 QLEAEAQKHC EALRREKEVS ATLKSTVEAL HTQKRELRCS LEREREKPAW LQAELEQSHP 2890 2900 2910 2920 2930 2940 RLKEQEGRKA ARRSAEARQS PAAAEQWRKW QRDKEKLREL ELQRQRDLHK IKQLQQTVRD 2950 2960 2970 2980 2990 3000 LESKDEVPGS RLHLGSARRA AGSDADHLRE QQRELEAMRQ RLLSAARLLT SFTSQAVDRT 3010 3020 3030 3040 3050 3060 VNDWTSSNEK AVMSLLHTLE ELKSDLSRPT SSQKKMAAEL QFQFVDVLLK DNVSLTKALS 3070 3080 3090 3100 3110 3120 TVTQEKLELS RAVSKLEKLL KHHLQKGCSP SRSERSAWKP DETAPQSSLR RPDPGRLPPA 3130 3140 3150 3160 3170 3180 ASEEAHTSNV KMEKLYLHYL RAESFRKALI YQKKYLLLLI GGFQDSEQET LSMIAHLGVF 3190 3200 3210 3220 3230 3240 PSKAERKITS RPFTRFRTAV RVVIAILRLR FLVKKWQEVD RKGALAQGKA PRPGPRARQP 3250 3260 3270 3280 3290 3300 QSPPRTRESP PTRDVPSGHT RDPARGRRLA AAASPHSGGR ATPSPNSRLE RSLTASQDPE 3310 3320 3330 HSLTEYIHHL EVIQQRLGGV LPDSTSKKSC HPMIKQ
	Isoform 2 [UniParc]. Checksum: A0FD227ABCF410FD	FASTA	3,139	355,882
	10 20 30 40 50 60 MFTVSDHPPE QHGMFTVGDH PPEQRGMFTV SDHPPEQHGM FTVSDHPPEQ RGMFTISDHQ 70 80 90 100 110 120 PEQRGMFTVS DHTPEQRGIF TISDHPAEQR GMFTKECEQE CELAITDLES GREDEAGLHQ 130 140 150 160 170 180 SQAVHGLELE ALRLSLSNMH TAQLELTQAN LQKEKETALT ELREMLNSRR AQELALLQSR 190 200 210 220 230 240 QQHELELLRE QHAREKEEVV LRCGQEAAEL KEKLQSEMEK NAQIVKTLKE DWESEKDLCL 250 260 270 280 290 300 ENLRKELSAK HQSEMEDLQN QFQKELAEQR AELEKIFQDK NQAERALRNL ESHHQAAIEK 310 320 330 340 350 360 LREDLQSEHG RCLEDLEFKF KESEKEKQLE LENLQASYED LKAQSQEEIR RLWSQLDSAR 370 380 390 400 410 420 TSRQELSELH EQLLARTSRV EDLEQLKQRE KTQHESELEQ LRIYFEKKLR DAEKTYQEDL 430 440 450 460 470 480 TLLQQRLQGA REDALLDSVE VGLSCVGLEE KPEKGRKDHV DELEPERHKE SLPRFQAELE 490 500 510 520 530 540 ESHRHQLEAL ESPLCIQHEG HVSDRCCVET SALGHEWRLE PSEGHSQELP WVHLQGVQDG 550 560 570 580 590 600 DLEADTERAA RVLGLETEHK VQLSLLQTEL KEEIELLKIE NRNLYGKLQH ETRLKDDLEK 610 620 630 640 650 660 VKHNLIEDHQ KELNNAKQKT ELMKQEFQRK ETDWKVMKEE LQREAEEKLT LMLLELREKA 670 680 690 700 710 720 ESEKQTIINK FELREAEMRQ LQDQQAAQIL DLERSLTEQQ GRLQQLEQDL TSDDALHCSQ 730 740 750 760 770 780 CGREPPTAQD GELAALHVKE DCALQLMLAR SRFLEERKEI TEKFSAEQDA FLQEAQEQHA 790 800 810 820 830 840 RELQLLQERH QQQLLSVTAE LEARHQAALG ELTASLESKQ GALLAARVAE LQTKHAADLG 850 860 870 880 890 900 ALETRHLSSL DSLESCYLSE FQTIREEHRQ ALELLRADFE EQLWKKDSLH QTILTQELEK 910 920 930 940 950 960 LKRKHEGELQ SVRDHLRTEV STELAGTVAH ELQGVHQGEF GSEKKTALHE KEETLRLQSA 970 980 990 1000 1010 1020 QAQPFHQEEK ESLSLQLQKK NHQVQQLKDQ VLSLSHEIEE CRSELEVLQQ RRERENREGA 1030 1040 1050 1060 1070 1080 NLLSMLKADV NLSHSERGAL QDALRRLLGL FGETLRAAVT LRSRIGERVG LCLDDAGAGL 1090 1100 1110 1120 1130 1140 ALSTAPALEE TWSDVALPEL DRTLSECAEM SSVAEISSHM RESFLMSPES VRECEQPIRR 1150 1160 1170 1180 1190 1200 VFQSLSLAVD GLMEMALDSS RQLEEARQIH SRFEKEFSFK NEETAQVVRK HQELLECLKE 1210 1220 1230 1240 1250 1260 ESAAKAELAL ELHKTQGTLE GFKVETADLK EVLAGKEDSE HRLVLELESL RRQLQQAAQE 1270 1280 1290 1300 1310 1320 QAALREECTR LWSRGEATAT DAEAREAALR KEVEDLTKEQ SETRKQAEKD RSALLSQMKI 1330 1340 1350 1360 1370 1380 LESELEEQLS QHRGCAKQAE AVTALEQQVA SLDKHLRNQR QFMDEQAAER EHEREEFQQE 1390 1400 1410 1420 1430 1440 IQRLEGQLRQ AAKPQPWGPR DSQQAPLDGE VELLQQKLRE KLDEFNELAI QKESADRQVL 1450 1460 1470 1480 1490 1500 MQEEEIKRLE EMNINIRKKV AQLQEEVEKQ KNIVKGLEQD KEVLKKQQMS SLLLASTLQS 1510 1520 1530 1540 1550 1560 TLDAGRCPEP PSGSPPEGPE IQLEVTQRAL LRRESEVLDL KEQLEKMKGD LESKNEEILH 1570 1580 1590 1600 1610 1620 LNLKLDMQNS QTAVSLRELE EENTSLKVIY TRSSEIEELK ATIENLQENQ KRLQKEKAEE 1630 1640 1650 1660 1670 1680 IEQLHEVIEK LQHELSLMGP VVHEVSDSQA GSLQSELLCS QAGGPRGQAL QGELEAALEA 1690 1700 1710 1720 1730 1740 KEALSRLLAD QERRHSQALE ALQQRLQGAE EAAELQLAEL ERNVALREAE VEDMASRIQE 1750 1760 1770 1780 1790 1800 FEAALKAKEA TIAERNLEID ALNQRKAAHS AELEAVLLAL ARIRRALEQQ PLAAGAAPPE 1810 1820 1830 1840 1850 1860 LQWLRAQCAR LSRQLQVLHQ RFLRCQVELD RRQARRATAH TRVPGAHPQP RMDGGAKAQV 1870 1880 1890 1900 1910 1920 TGDVEASHDA ALEPVVPDPQ GDLQPVLVTL KDAPLCKQEG VMSVLTVCQR QLQSELLLVK 1930 1940 1950 1960 1970 1980 NEMRLSLEDG GKGKEKVLED CQLPKVDLVA QVKQLQEKLN RLLYSMTFQN VDAADTKSLW 1990 2000 2010 2020 2030 2040 PMASAHLLES SWSDDSCDGE EPDISPHIDT CDANTATGGV TDVIKNQAID ACDANTTPGG 2050 2060 2070 2080 2090 2100 VTDVIKNWDS LIPDEMPDSP IQEKSECQDM SLSSPTSVLG GSRHQSHTAE AGPRKSPVGM 2110 2120 2130 2140 2150 2160 LDLSSWSSPE VLRKDWTLEP WPSLPVTPHS GALSLCSADT SLGDRADTSL PQTQGPGLLC 2170 2180 2190 2200 2210 2220 SPGVSAAALA LQWAESPPAD DHHVQRTAVE KDVEDFITTS FDSQETLSSP PPGLEGKADR 2230 2240 2250 2260 2270 2280 SEKSDGSGFG ARLSPGSGGP EAQTAGPVTP ASISGRFQPL PEAMKEKEVR PKHVKALLQM 2290 2300 2310 2320 2330 2340 VRDESHQILA LSEGLAPPSG EPHPPRKEDE IQDISLHGGK TQEVPTACPD WRGDLLQVVQ 2350 2360 2370 2380 2390 2400 EAFEKEQEMQ GVELQPRLSG SDLGGHSSLL ERLEKIIREQ GDLQEKSLEH LRLPDRSSLL 2410 2420 2430 2440 2450 2460 SEIQALRAQL RMTHLQNQEK LQHLRTALTS AEARGSQQEH QLRRQVELLA YKVEQEKCIA 2470 2480 2490 2500 2510 2520 GDLQKTLSEE QEKANSVQKL LAAEQTVVRD LKSDLCESRQ KSEQLSRSLC EVQQEVLQLR 2530 2540 2550 2560 2570 2580 SMLSSKENEL KAALQELESE QGKGRALQSQ LEEEQLRHLQ RESQSAKALE ELRASLETQR 2590 2600 2610 2620 2630 2640 AQSSRLCVAL KHEQTAKDNL QKELRIEHSR CEALLAQERS QLSELQKDLA AEKSRTLELS 2650 2660 2670 2680 2690 2700 EALRHERLLT EQLSQRTQEA CVHQDTQAHH ALLQKLKEEK SRVVDLQAML EKVQQQALHS 2710 2720 2730 2740 2750 2760 QQQLEAEAQK HCEALRREKE RELELQRQRD LHKIKQLQQT VRDLESKDEV PGSRLHLGSA 2770 2780 2790 2800 2810 2820 RRAAGSDADH LREQQRELEA MRQRLLSAAR LLTSFTSQAV DRTVNDWTSS NEKAVMSLLH 2830 2840 2850 2860 2870 2880 TLEELKSDLS RPTSSQKKMA AELQFQFVDV LLKDNVSLTK ALSTVTQEKL ELSRAVSKLE 2890 2900 2910 2920 2930 2940 KLLKHHLQKG CSPSRSERSA WKPDETAPQS SLRRPDPGRL PPAASEEAHT SNVKMEKLYL 2950 2960 2970 2980 2990 3000 HYLRAESFRK ALIYQKKYLL LLIGGFQDSE QETLSMIAHL GVFPSKAERK ITSRPFTRFR 3010 3020 3030 3040 3050 3060 TAVRVVIAIL RLRFLVKKWQ EVDRKGALAQ GKAPRPGPRA RQPQSPPRTR ESPPTRDVPS 3070 3080 3090 3100 3110 3120 GHTRDPARGR RLAAAASPHS GGRATPSPNS RLERSLTASQ DPEHSLTEYI HHLEVIQQRL 3130 GGVLPDSTSK KSCHPMIKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1." Li Q., Hansen D., Killilea A., Joshi H.C., Palazzo R.E., Balczon R. J. Cell Sci. 114:797-809(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, VARIANTS GLU-704; ALA-879; ALA-1038; ARG-2188 AND THR-2549.
[2]	"Vertebrate centrosome proteins that share homology with yeast mitotic exit proteins are required for cytokinesis and cell cycle progression." Gromley A.S., Jurczyk A., Sillibourne J.E., Halilovic E., Doxsey S.J. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLU-704.
[3]	"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro." Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS GLU-704 AND ALA-1038. Tissue: Brain.
[4]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 3023.
[5]	Ohara O. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[6]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 787-1533 (ISOFORM 1), VARIANT ALA-1038. Tissue: Trachea.
[8]	"Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin." Flory M.R., Moser M.J., Monnat R.J. Jr., Davis T.N. Proc. Natl. Acad. Sci. U.S.A. 97:5919-5923(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, CALMODULIN-BINDING DOMAIN, MUTAGENESIS OF 3196-PHE-ARG-3197; VAL-3203 AND 3208-ARG-LEU-3209.
[9]	"DISC1-kendrin interaction is involved in centrosomal microtubule network formation." Shimizu S., Matsuzaki S., Hattori T., Kumamoto N., Miyoshi K., Katayama T., Tohyama M. Biochem. Biophys. Res. Commun. 377:1051-1056(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DISC1, SUBCELLULAR LOCATION.
[10]	"Mutations in pericentrin cause Seckel syndrome with defective ATR-dependent DNA damage signaling." Griffith E., Walker S., Martin C.-A., Vagnarelli P., Stiff T., Vernay B., Al Sanna N., Saggar A., Hamel B., Earnshaw W.C., Jeggo P.A., Jackson A.P., O'Driscoll M. Nat. Genet. 40:232-236(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN MOPD2.
[11]	"Mutations in the pericentrin (PCNT) gene cause primordial dwarfism." Rauch A., Thiel C.T., Schindler D., Wick U., Crow Y.J., Ekici A.B., van Essen A.J., Goecke T.O., Al-Gazali L., Chrzanowska K.H., Zweier C., Brunner H.G., Becker K., Curry C.J., Dallapiccola B., Devriendt K., Doerfler A., Kinning E. , Megarbane A., Meinecke P., Semple R.K., Spranger S., Toutain A., Trembath R.C., Voss E., Wilson L., Hennekam R., de Zegher F., Doerr H.-G., Reis A. Science 319:816-819(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN MOPD2.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2177 AND SER-2327, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Involvement of a centrosomal protein kendrin in the maintenance of centrosome cohesion by modulating Nek2A kinase activity." Matsuo K., Nishimura T., Hayakawa A., Ono Y., Takahashi M. Biochem. Biophys. Res. Commun. 398:217-223(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NEK2.
[14]	"Conserved motif of CDK5RAP2 mediates its localization to centrosomes and the Golgi complex." Wang Z., Wu T., Shi L., Zhang L., Zheng W., Qu J.Y., Niu R., Qi R.Z. J. Biol. Chem. 285:22658-22665(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDK5RAP2.
[15]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-191; SER-682; SER-2044; SER-2477; SER-2486 AND SER-3302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[16]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52962 mRNA. Translation: AAD10838.1. Frameshift. AF515282 mRNA. Translation: AAP46636.1. AB007862 mRNA. Translation: BAA23698.3. Different initiation. AP000471 Genomic DNA. No translation available. AP001477 Genomic DNA. No translation available. AP000335 Genomic DNA. No translation available. AP000336 Genomic DNA. No translation available. AP000337 Genomic DNA. No translation available. AK093923 mRNA. Translation: BAC04252.1. Different initiation.
IPI	IPI00479143. IPI00973279.
RefSeq	NP_006022.3. NM_006031.5.
UniGene	Hs.474069.
3D structure databases
ProteinModelPortal	O95613.
ModBase	Search...
Protein-protein interaction databases
IntAct	O95613. 3 interactions.
STRING	9606.ENSP00000352572.
PTM databases
PhosphoSite	O95613.
Proteomic databases
PaxDb	O95613.
PRIDE	O95613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359568; ENSP00000352572; ENSG00000160299.
GeneID	5116.
KEGG	hsa:5116.
UCSC	uc002zji.4. human. uc002zjj.3. human.
Organism-specific databases
CTD	5116.
GeneCards	GC21P047744.
H-InvDB	HIX0203117.
HGNC	HGNC:16068. PCNT.
HPA	HPA016820. HPA019887.
MIM	210720. phenotype. 605925. gene.
neXtProt	NX_O95613.
Orphanet	2637. Microcephalic osteodysplastic primordial short stature type 2. 808. Seckel syndrome.
PharmGKB	PA33079.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000168229.
HOVERGEN	HBG079443.
InParanoid	O95613.
KO	K16481.
OMA	EQRGMFT.
OrthoDB	EOG4PNXG1.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle.
Gene expression databases
ArrayExpress	O95613.
Bgee	O95613.
CleanEx	HS_PCNT.
Genevestigator	O95613.
GermOnline	ENSG00000160299. Homo sapiens.
Family and domain databases
InterPro	IPR019528. PACT_domain. IPR024151. Pericentrin. [Graphical view]
PANTHER	PTHR18932:SF3. PTHR18932:SF3. 1 hit.
Pfam	PF10495. PACT_coil_coil. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PCNT. human.
GenomeRNAi	5116.
NextBio	19730.
SOURCE	Search...

Entry information

Entry name

PCNT_HUMAN

Accession

Primary (citable) accession number: O95613
Secondary accession number(s): O43152, Q7Z7C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 125 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.