UniProtKB - O95600 (KLF8_HUMAN)
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Protein
Krueppel-like factor 8
Gene
KLF8
Organism
Homo sapiens (Human)
Status
Functioni
Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G1 phase as a downstream target of focal adhesion kinase (FAK).3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 274 – 298 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 304 – 328 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 334 – 356 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
- transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: NTNU_SB
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
SIGNORi | O95600. |
Names & Taxonomyi
Protein namesi | Recommended name: Krueppel-like factor 8Alternative name(s): Basic krueppel-like factor 3 Zinc finger protein 741 |
Gene namesi | Name:KLF8 Synonyms:BKLF3, ZNF741 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000102349.15. |
HGNCi | HGNC:6351. KLF8. |
MIMi | 300286. gene. |
neXtProti | NX_O95600. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 67 | K → R: Abolishes sumoylation. No change in nuclear location. Increases transcriptional activity and cell cycle progression. Abolishes sumoylation; when associated with R-217. 1 Publication | 1 | |
Mutagenesisi | 217 | K → R: No change in sumoylation. Abolishes sumoylation; when associated with R-67. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 11279. |
OpenTargetsi | ENSG00000102349. |
PharmGKBi | PA30141. |
Polymorphism and mutation databases
BioMutai | KLF8. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047176 | 1 – 359 | Krueppel-like factor 8Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 67 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) |
Post-translational modificationi
Sumoylation at Lys-67 represses transcriptional activity and reduces cell cycle progression into the G1 phase. Has no effect on subcellular location.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | O95600. |
PeptideAtlasi | O95600. |
PRIDEi | O95600. |
PTM databases
iPTMneti | O95600. |
PhosphoSitePlusi | O95600. |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000102349. |
CleanExi | HS_KLF8. |
ExpressionAtlasi | O95600. baseline and differential. |
Genevisiblei | O95600. HS. |
Organism-specific databases
HPAi | HPA071740. |
Interactioni
Subunit structurei
Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2, AND PIAS4; the interaction with each ligase sumoylates KLF8.2 Publications
Protein-protein interaction databases
BioGridi | 116435. 13 interactors. |
ELMi | O95600. |
IntActi | O95600. 6 interactors. |
MINTi | O95600. |
STRINGi | 9606.ENSP00000417303. |
Structurei
3D structure databases
ProteinModelPortali | O95600. |
SMRi | O95600. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the Sp1 C2H2-type zinc-finger protein family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 274 – 298 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 304 – 328 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 334 – 356 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721. Eukaryota. COG5048. LUCA. |
GeneTreei | ENSGT00760000118998. |
HOVERGENi | HBG003941. |
InParanoidi | O95600. |
KOi | K09205. |
OMAi | PAAMTQM. |
OrthoDBi | EOG091G1BN0. |
PhylomeDBi | O95600. |
TreeFami | TF350556. |
Family and domain databases
InterProi | View protein in InterPro IPR036236. Znf_C2H2_sf. IPR013087. Znf_C2H2_type. |
SMARTi | View protein in SMART SM00355. ZnF_C2H2. 3 hits. |
SUPFAMi | SSF57667. SSF57667. 2 hits. |
PROSITEi | View protein in PROSITE PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: O95600-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MVDMDKLINN LEVQLNSEGG SMQVFKQVTA SVRNRDPPEI EYRSNMTSPT
60 70 80 90 100
LLDANPMENP ALFNDIKIEP PEELLASDFS LPQVEPVDLS FHKPKAPLQP
110 120 130 140 150
ASMLQAPIRP PKPQSSPQTL VVSTSTSDMS TSANIPTVLT PGSVLTSSQS
160 170 180 190 200
TGSQQILHVI HTIPSVSLPN KMGGLKTIPV VVQSLPMVYT TLPADGGPAA
210 220 230 240 250
ITVPLIGGDG KNAGSVKVDP TSMSPLEIPS DSEESTIESG SSALQSLQGL
260 270 280 290 300
QQEPAAMAQM QGEESLDLKR RRIHQCDFAG CSKVYTKSSH LKAHRRIHTG
310 320 330 340 350
EKPYKCTWDG CSWKFARSDE LTRHFRKHTG IKPFRCTDCN RSFSRSDHLS
LHRRRHDTM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 167 | S → I in BAG58895 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 263 | E → G in AAC99849 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047480 | 1 – 27 | MVDMD…QVFKQ → MSLPEDGMSSGHFRSPQLVT WS in isoform 3. 1 PublicationAdd BLAST | 27 | |
Alternative sequenceiVSP_047481 | 216 – 299 | Missing in isoform 4. 1 PublicationAdd BLAST | 84 | |
Alternative sequenceiVSP_045460 | 254 – 359 | PAAMA…RHDTM → REAL in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 106 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28282 mRNA. Translation: AAC99849.1. HF546207 mRNA. Translation: CCO02793.1. HF546208 mRNA. Translation: CCO02794.1. HF546209 mRNA. Translation: CCO02795.1. AK296156 mRNA. Translation: BAG58895.1. AB209004 mRNA. Translation: BAD92241.1. Sequence problems. BX641066 mRNA. Translation: CAE46033.1. Sequence problems. BX322609, AL050309 Genomic DNA. Translation: CAI41397.1. AL050309, BX322609 Genomic DNA. Translation: CAI42343.1. BC105130 mRNA. Translation: AAI05131.1. BC112109 mRNA. Translation: AAI12110.1. |
CCDSi | CCDS14373.1. [O95600-1] CCDS55428.1. [O95600-3] |
RefSeqi | NP_001152768.1. NM_001159296.2. [O95600-3] NP_001311028.1. NM_001324099.1. [O95600-4] NP_001311029.1. NM_001324100.1. [O95600-5] NP_001311031.1. NM_001324102.1. [O95600-1] NP_009181.2. NM_007250.5. [O95600-1] XP_005262034.1. XM_005261977.2. [O95600-1] XP_005262036.1. XM_005261979.3. [O95600-1] XP_006724638.1. XM_006724575.2. [O95600-3] |
UniGenei | Hs.646614. |
Genome annotation databases
Ensembli | ENST00000374928; ENSP00000364063; ENSG00000102349. [O95600-3] ENST00000468660; ENSP00000417303; ENSG00000102349. [O95600-1] ENST00000640927; ENSP00000492126; ENSG00000102349. [O95600-4] |
GeneIDi | 11279. |
KEGGi | hsa:11279. |
UCSCi | uc004dur.4. human. [O95600-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | KLF8_HUMAN | |
Accessioni | O95600Primary (citable) accession number: O95600 Secondary accession number(s): B4DJN3 Q9UGC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | November 2, 2001 | |
Last modified: | March 28, 2018 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |