Reviewed,
UniProtKB/Swiss-Prot O95573 (ACSL3_HUMAN)
Last modified
November 3, 2009.
Version 86.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Long-chain-fatty-acid--CoA ligase 3 EC=6.2.1.3 Alternative name(s): Long-chain acyl-CoA synthetase 3 Short name=LACS 3 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 720 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis By similarity. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates By similarity. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). |
| Catalytic activity | ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA. |
| Cofactor | Magnesium By similarity. |
| Subcellular location | Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity. |
| Sequence similarities | Belongs to the ATP-dependent AMP-binding enzyme family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 720 | 720 | Long-chain-fatty-acid--CoA ligase 3 | PRO_0000193107 | |||||
Regions | |||||||||
| Transmembrane | 21 – 41 | 21 | Signal-anchor for type III membrane protein Potential | ||||||
| Topological domain | 42 – 720 | 679 | Cytoplasmic Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 593 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 683 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 688 | 1 | Phosphothreonine Ref.6 | ||||||
Natural variations | |||||||||
| Natural variant | 551 | 1 | F → S: dbSNP rs1046032. Ref.1 Ref.2 | VAR_026716 | |||||
Experimental info | |||||||||
| Sequence conflict | 246 | 1 | E → D in BAA37142. Ref.1 | ||||||
| Sequence conflict | 246 | 1 | E → D in BAB72074. Ref.2 | ||||||
| Sequence conflict | 246 | 1 | E → D in BAB72139. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human acyl-coenzyme A synthetase 3 cDNA and localization of its gene (ACS3) to chromosome band 2q34-q35." Minekura H., Fujino T., Kang M.-J., Fujita T., Endo Y., Yamamoto T.T. Genomics 42:180-181(1997) [PubMed: 9177793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-551. Tissue: Placenta. |
| [2] | "Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene." Minekura H., Kang M.-J., Inagaki Y., Suzuki H., Sato H., Fujino T., Yamamoto T.T. Gene 278:185-192(2001) [PubMed: 11707336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT SER-551. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas. |
| [4] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593, MASS SPECTROMETRY. Tissue: Epithelium. |
| [5] | "Long chain acyl-CoA synthetase 3-mediated phosphatidylcholine synthesis is required for assembly of very low density lipoproteins in human hepatoma Huh7 cells." Yao H., Ye J. J. Biol. Chem. 283:849-854(2008) [PubMed: 18003621] [Abstract] Cited for: FUNCTION. |
| [6] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-683 AND THR-688, MASS SPECTROMETRY. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D89053 mRNA. Translation: BAA37142.1. AB061712 mRNA. Translation: BAB72074.1. AB061436 Genomic DNA. Translation: BAB72139.1. BC041692 mRNA. Translation: AAH41692.1. | |
| IPI | IPI00031397. |
| RefSeq | NP_004448.2. NP_976251.1. |
| UniGene | Hs.655772 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O95573. 1 interaction. |
| STRING | O95573. |
PTM databases | |
| PhosphoSite | O95573. |
Proteomic databases | |
| PeptideAtlas | O95573. |
| PRIDE | O95573. |
Genome annotation databases | |
| Ensembl | ENST00000357430; ENSP00000350012; ENSG00000123983; Homo sapiens. [Genome view] ENST00000392066; ENSP00000375918; ENSG00000123983; Homo sapiens. [Genome view] ENST00000407441; ENSP00000385157; ENSG00000123983; Homo sapiens. [Genome view] ENST00000413316; ENSP00000416913; ENSG00000123983; Homo sapiens. [Genome view] ENST00000421680; ENSP00000404182; ENSG00000123983; Homo sapiens. [Genome view] |
| GeneID | 2181. |
| KEGG | hsa:2181. |
| UCSC | uc002vni.1. human. |
Organism-specific databases | |
| CTD | 2181. |
| GeneCards | GC02P223433. |
| HGNC | HGNC:3570. ACSL3. |
| HPA | HPA011315. |
| MIM | 602371. gene. |
| PharmGKB | PA27967. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | O95573. |
| HOVERGEN | O95573. |
| OMA | NKFKDKR. |
Enzyme and pathway databases | |
| BRENDA | 6.2.1.3. 247. |
Gene expression databases | |
| ArrayExpress | O95573. |
| Bgee | O95573. |
| CleanEx | HS_ACSL3. |
| Genevestigator | O95573. |
| GermOnline | ENSG00000123983. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000873. AMP-dep_Synth/Lig. [Graphical view] |
| Pfam | PF00501. AMP-binding. 1 hit. [Graphical view] |
| PROSITE | PS00455. AMP_BINDING. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| DrugBank | DB00159. Icosapent. |
| NextBio | 8805. |
| SOURCE | Search... |
Entry information
| Entry name | ACSL3_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O95573 Secondary accession number(s): Q60I92, Q8IUM9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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