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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene

ACSL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
  3. protein domain specific binding Source: UniProtKB
  4. protein kinase binding Source: UniProtKB

GO - Biological processi

  1. brain development Source: Ensembl
  2. fatty acid biosynthetic process Source: Ensembl
  3. long-chain fatty acid import Source: UniProtKB
  4. long-chain fatty acid metabolic process Source: GOC
  5. positive regulation of Golgi to plasma membrane protein transport Source: UniProtKB
  6. positive regulation of phosphatidylcholine biosynthetic process Source: UniProtKB
  7. positive regulation of secretion Source: UniProtKB
  8. response to nutrient Source: Ensembl
  9. response to organic cyclic compound Source: Ensembl
  10. very-low-density lipoprotein particle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04703-MONOMER.
BRENDAi6.2.1.3. 2681.
ReactomeiREACT_380. Synthesis of very long-chain fatty acyl-CoAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 3
Short name:
LACS 3
Gene namesi
Name:ACSL3
Synonyms:ACS3, FACL3, LACS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:3570. ACSL3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
BLAST
Topological domaini42 – 720679CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. Golgi apparatus Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
  5. lipid particle Source: UniProtKB
  6. membrane Source: UniProtKB
  7. mitochondrial outer membrane Source: UniProtKB-SubCell
  8. perinuclear region of cytoplasm Source: UniProtKB
  9. peroxisomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27967.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Long-chain-fatty-acid--CoA ligase 3PRO_0000193107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei683 – 6831Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO95573.
PaxDbiO95573.
PeptideAtlasiO95573.
PRIDEiO95573.

PTM databases

PhosphoSiteiO95573.

Expressioni

Gene expression databases

BgeeiO95573.
CleanExiHS_ACSL3.
ExpressionAtlasiO95573. baseline and differential.
GenevestigatoriO95573.

Organism-specific databases

HPAiHPA011315.

Interactioni

Protein-protein interaction databases

BioGridi108477. 22 interactions.
IntActiO95573. 11 interactions.
MINTiMINT-4527745.
STRINGi9606.ENSP00000350012.

Structurei

3D structure databases

ProteinModelPortaliO95573.
SMRiO95573. Positions 122-641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiO95573.
KOiK01897.
OMAiCDRFIFR.
OrthoDBiEOG7P2XRD.
PhylomeDBiO95573.
TreeFamiTF314012.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O95573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNHVSSKPS TMKLKHTINP ILLYFIHFLI SLYTILTYIP FYFFSESRQE
60 70 80 90 100
KSNRIKAKPV NSKPDSAYRS VNSLDGLASV LYPGCDTLDK VFTYAKNKFK
110 120 130 140 150
NKRLLGTREV LNEEDEVQPN GKIFKKVILG QYNWLSYEDV FVRAFNFGNG
160 170 180 190 200
LQMLGQKPKT NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
ALNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
IIVHTMAAVE ALGAKASMEN QPHSKPLPSD IAVIMYTSGS TGLPKGVMIS
310 320 330 340 350
HSNIIAGITG MAERIPELGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSMLKPTLM AAVPEIMDRI YKNVMNKVSE
410 420 430 440 450
MSSFQRNLFI LAYNYKMEQI SKGRNTPLCD SFVFRKVRSL LGGNIRLLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES AGAGTISEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQSVTMGYY KNEAKTKADF
560 570 580 590 600
FEDENGQRWL CTGDIGEFEP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLVDNI CAYANSYHSY VIGFVVPNQK ELTELARKKG LKGTWEELCN
660 670 680 690 700
SCEMENEVLK VLSEAAISAS LEKFEIPVKI RLSPEPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK
Length:720
Mass (Da):80,420
Last modified:October 2, 2006 - v3
Checksum:iAAC4B0B4543EC8DD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461E → D in BAA37142 (PubMed:9177793).Curated
Sequence conflicti246 – 2461E → D in BAB72074 (PubMed:11707336).Curated
Sequence conflicti246 – 2461E → D in BAB72139 (PubMed:11707336).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti551 – 5511F → S.2 Publications
Corresponds to variant rs1046032 [ dbSNP | Ensembl ].
VAR_026716

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89053 mRNA. Translation: BAA37142.1.
AB061712 mRNA. Translation: BAB72074.1.
AB061436 Genomic DNA. Translation: BAB72139.1.
BC041692 mRNA. Translation: AAH41692.1.
CCDSiCCDS2455.1.
RefSeqiNP_004448.2. NM_004457.3.
NP_976251.1. NM_203372.1.
UniGeneiHs.655772.

Genome annotation databases

EnsembliENST00000357430; ENSP00000350012; ENSG00000123983.
ENST00000392066; ENSP00000375918; ENSG00000123983.
GeneIDi2181.
KEGGihsa:2181.
UCSCiuc002vni.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89053 mRNA. Translation: BAA37142.1.
AB061712 mRNA. Translation: BAB72074.1.
AB061436 Genomic DNA. Translation: BAB72139.1.
BC041692 mRNA. Translation: AAH41692.1.
CCDSiCCDS2455.1.
RefSeqiNP_004448.2. NM_004457.3.
NP_976251.1. NM_203372.1.
UniGeneiHs.655772.

3D structure databases

ProteinModelPortaliO95573.
SMRiO95573. Positions 122-641.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108477. 22 interactions.
IntActiO95573. 11 interactions.
MINTiMINT-4527745.
STRINGi9606.ENSP00000350012.

Chemistry

DrugBankiDB00159. Icosapent.

PTM databases

PhosphoSiteiO95573.

Proteomic databases

MaxQBiO95573.
PaxDbiO95573.
PeptideAtlasiO95573.
PRIDEiO95573.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357430; ENSP00000350012; ENSG00000123983.
ENST00000392066; ENSP00000375918; ENSG00000123983.
GeneIDi2181.
KEGGihsa:2181.
UCSCiuc002vni.3. human.

Organism-specific databases

CTDi2181.
GeneCardsiGC02P223725.
HGNCiHGNC:3570. ACSL3.
HPAiHPA011315.
MIMi602371. gene.
neXtProtiNX_O95573.
PharmGKBiPA27967.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1022.
GeneTreeiENSGT00690000102168.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiO95573.
KOiK01897.
OMAiCDRFIFR.
OrthoDBiEOG7P2XRD.
PhylomeDBiO95573.
TreeFamiTF314012.

Enzyme and pathway databases

BioCyciMetaCyc:HS04703-MONOMER.
BRENDAi6.2.1.3. 2681.
ReactomeiREACT_380. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

ChiTaRSiACSL3. human.
GeneWikiiACSL3.
GenomeRNAii2181.
NextBioi8805.
PROiO95573.
SOURCEiSearch...

Gene expression databases

BgeeiO95573.
CleanExiHS_ACSL3.
ExpressionAtlasiO95573. baseline and differential.
GenevestigatoriO95573.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human acyl-coenzyme A synthetase 3 cDNA and localization of its gene (ACS3) to chromosome band 2q34-q35."
    Minekura H., Fujino T., Kang M.-J., Fujita T., Endo Y., Yamamoto T.T.
    Genomics 42:180-181(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-551.
    Tissue: Placenta.
  2. "Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene."
    Minekura H., Kang M.-J., Inagaki Y., Suzuki H., Sato H., Fujino T., Yamamoto T.T.
    Gene 278:185-192(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT SER-551.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreas.
  4. "Long chain acyl-CoA synthetase 3-mediated phosphatidylcholine synthesis is required for assembly of very low density lipoproteins in human hepatoma Huh7 cells."
    Yao H., Ye J.
    J. Biol. Chem. 283:849-854(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-683, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACSL3_HUMAN
AccessioniPrimary (citable) accession number: O95573
Secondary accession number(s): Q60I92, Q8IUM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2001
Last sequence update: October 2, 2006
Last modified: March 3, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.