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Protein

Long-chain-fatty-acid--CoA ligase 3

Gene

ACSL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).By similarity1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate--CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • brain development Source: Ensembl
  • fatty acid biosynthetic process Source: Ensembl
  • long-chain fatty acid import Source: UniProtKB
  • long-chain fatty-acyl-CoA biosynthetic process Source: Reactome
  • positive regulation of Golgi to plasma membrane protein transport Source: UniProtKB
  • positive regulation of phosphatidylcholine biosynthetic process Source: UniProtKB
  • positive regulation of secretion Source: UniProtKB
  • response to nutrient Source: Ensembl
  • response to organic cyclic compound Source: Ensembl
  • very-low-density lipoprotein particle assembly Source: UniProtKB

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04703-MONOMER.
BRENDAi6.2.1.3. 2681.
ReactomeiR-HSA-75876. Synthesis of very long-chain fatty acyl-CoAs.

Chemistry databases

SwissLipidsiSLP:000000200.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 3 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 3
Short name:
LACS 3
Gene namesi
Name:ACSL3
Synonyms:ACS3, FACL3, LACS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:3570. ACSL3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini42 – 720CytoplasmicSequence analysisAdd BLAST679

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
  • Golgi apparatus Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lipid particle Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB
  • peroxisomal membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi2181.
OpenTargetsiENSG00000123983.
PharmGKBiPA27967.

Chemistry databases

DrugBankiDB00159. Icosapent.

Polymorphism and mutation databases

BioMutaiACSL3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931071 – 720Long-chain-fatty-acid--CoA ligase 3Add BLAST720

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei683PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95573.
MaxQBiO95573.
PaxDbiO95573.
PeptideAtlasiO95573.
PRIDEiO95573.

PTM databases

iPTMnetiO95573.
PhosphoSitePlusiO95573.
SwissPalmiO95573.

Expressioni

Gene expression databases

BgeeiENSG00000123983.
CleanExiHS_ACSL3.
ExpressionAtlasiO95573. baseline and differential.
GenevisibleiO95573. HS.

Organism-specific databases

HPAiHPA011315.
HPA071021.

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108477. 37 interactors.
IntActiO95573. 37 interactors.
MINTiMINT-4527745.
STRINGi9606.ENSP00000350012.

Structurei

3D structure databases

ProteinModelPortaliO95573.
SMRiO95573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00870000136474.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiO95573.
KOiK01897.
OMAiHDINRMY.
OrthoDBiEOG091G02F4.
PhylomeDBiO95573.
TreeFamiTF314012.

Family and domain databases

InterProiView protein in InterPro
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

O95573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNHVSSKPS TMKLKHTINP ILLYFIHFLI SLYTILTYIP FYFFSESRQE
60 70 80 90 100
KSNRIKAKPV NSKPDSAYRS VNSLDGLASV LYPGCDTLDK VFTYAKNKFK
110 120 130 140 150
NKRLLGTREV LNEEDEVQPN GKIFKKVILG QYNWLSYEDV FVRAFNFGNG
160 170 180 190 200
LQMLGQKPKT NIAIFCETRA EWMIAAQACF MYNFQLVTLY ATLGGPAIVH
210 220 230 240 250
ALNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK PPTWSEFPKG
260 270 280 290 300
IIVHTMAAVE ALGAKASMEN QPHSKPLPSD IAVIMYTSGS TGLPKGVMIS
310 320 330 340 350
HSNIIAGITG MAERIPELGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG
360 370 380 390 400
YSSPQTLADQ SSKIKKGSKG DTSMLKPTLM AAVPEIMDRI YKNVMNKVSE
410 420 430 440 450
MSSFQRNLFI LAYNYKMEQI SKGRNTPLCD SFVFRKVRSL LGGNIRLLLC
460 470 480 490 500
GGAPLSATTQ RFMNICFCCP VGQGYGLTES AGAGTISEVW DYNTGRVGAP
510 520 530 540 550
LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQSVTMGYY KNEAKTKADF
560 570 580 590 600
FEDENGQRWL CTGDIGEFEP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA
610 620 630 640 650
LKNLPLVDNI CAYANSYHSY VIGFVVPNQK ELTELARKKG LKGTWEELCN
660 670 680 690 700
SCEMENEVLK VLSEAAISAS LEKFEIPVKI RLSPEPWTPE TGLVTDAFKL
710 720
KRKELKTHYQ ADIERMYGRK
Length:720
Mass (Da):80,420
Last modified:October 3, 2006 - v3
Checksum:iAAC4B0B4543EC8DD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246E → D in BAA37142 (PubMed:9177793).Curated1
Sequence conflicti246E → D in BAB72074 (PubMed:11707336).Curated1
Sequence conflicti246E → D in BAB72139 (PubMed:11707336).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_026716551F → S2 PublicationsCorresponds to variant dbSNP:rs1046032Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89053 mRNA. Translation: BAA37142.1.
AB061712 mRNA. Translation: BAB72074.1.
AB061436 Genomic DNA. Translation: BAB72139.1.
BC041692 mRNA. Translation: AAH41692.1.
CCDSiCCDS2455.1.
RefSeqiNP_004448.2. NM_004457.3.
NP_976251.1. NM_203372.1.
XP_016859073.1. XM_017003584.1.
UniGeneiHs.655772.

Genome annotation databases

EnsembliENST00000357430; ENSP00000350012; ENSG00000123983.
ENST00000392066; ENSP00000375918; ENSG00000123983.
GeneIDi2181.
KEGGihsa:2181.
UCSCiuc002vni.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiACSL3_HUMAN
AccessioniPrimary (citable) accession number: O95573
Secondary accession number(s): Q60I92, Q8IUM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 3, 2006
Last modified: August 30, 2017
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families