O95544 (NADK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD kinase
EC=2.7.1.23

Alternative name(s):
Poly(P)/ATP NAD kinase

Gene names

Name:

NADK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + NAD+ = ADP + NADP+. Ref.1
Cofactor	Divalent metal cations. Ref.1
Tissue specificity	Widely expressed but not detected in skeletal muscle. Ref.1
Sequence similarities	Belongs to the NAD kinase family.
Biophysicochemical properties	Kinetic parameters: KM=3.3 mM for ATP Ref.1 KM=0.54 mM for NAD Vmax=6.7 µmol/min/mg enzyme pH dependence: Optimum pH is 7.5. Temperature dependence: Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Metal-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP metabolic process Non-traceable author statement Ref.1. Source: UniProtKB NAD metabolic process Traceable author statement. Source: Reactome NADP biosynthetic process Inferred from electronic annotation. Source: InterPro water-soluble vitamin metabolic process Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement Ref.1. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from direct assay Ref.1. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 446

446

NAD kinase

PRO_0000120713

Regions

Compositional bias

326 – 333

8

Poly-Ala

Compositional bias

437 – 445

9

Poly-Glu

Amino acid modifications

Modified residue

46

1

Phosphoserine Ref.6 Ref.7

Modified residue

48

1

Phosphoserine Ref.6 Ref.7

Modified residue

50

1

Phosphoserine Ref.6

Modified residue

64

1

Phosphoserine Ref.6

Natural variations

Natural variant

262

1

N → K. Ref.1 Ref.5
Corresponds to variant rs4751 [ dbSNP | Ensembl ].

VAR_034119

Experimental info

Sequence conflict

445

1

E → EE in BAB14412. Ref.2

Secondary structure

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446

Helix Strand Turn

Details...

Turn

75 – 77

3

Beta strand

78 – 80

3

Turn

84 – 86

3

Beta strand

87 – 91

5

Turn

93 – 95

3

Beta strand

97 – 102

6

Beta strand

106 – 111

6

Helix

116 – 118

3

Helix

119 – 131

13

Beta strand

135 – 139

5

Helix

140 – 144

5

Helix

146 – 150

5

Helix

155 – 160

6

Beta strand

162 – 164

3

Turn

166 – 168

3

Turn

172 – 174

3

Beta strand

176 – 183

8

Helix

186 – 193

8

Beta strand

195 – 197

3

Beta strand

201 – 208

8

Turn

210 – 212

3

Beta strand

215 – 217

3

Helix

220 – 229

10

Beta strand

233 – 237

5

Beta strand

240 – 245

6

Beta strand

277 – 289

13

Beta strand

299 – 303

5

Beta strand

306 – 311

6

Beta strand

313 – 319

7

Helix

321 – 325

5

Helix

327 – 330

4

Beta strand

343 – 349

7

Beta strand

358 – 360

3

Beta strand

366 – 370

5

Beta strand

378 – 382

5

Beta strand

385 – 390

6

Beta strand

395 – 400

6

Beta strand

405 – 408

4

Helix

413 – 424

12

Sequences

	Sequence		Length	Mass (Da)
	O95544 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 48CE7AF05EDD7E8B	FASTA	446	49,228
	10 20 30 40 50 60 MEMEQEKMTM NKELSPDAAA YCCSACHGDE TWSYNHPIRG RAKSRSLSAS PALGSTKEFR 70 80 90 100 110 120 RTRSLHGPCP VTTFGPKACV LQNPQTIMHI QDPASQRLTW NKSPKSVLVI KKMRDASLLQ 130 140 150 160 170 180 PFKELCTHLM EENMIVYVEK KVLEDPAIAS DESFGAVKKK FCTFREDYDD ISNQIDFIIC 190 200 210 220 230 240 LGGDGTLLYA SSLFQGSVPP VMAFHLGSLG FLTPFSFENF QSQVTQVIEG NAAVVLRSRL 250 260 270 280 290 300 KVRVVKELRG KKTAVHNGLG ENGSQAAGLD MDVGKQAMQY QVLNEVVIDR GPSSYLSNVD 310 320 330 340 350 360 VYLDGHLITT VQGDGVIVST PTGSTAYAAA AGASMIHPNV PAIMITPICP HSLSFRPIVV 370 380 390 400 410 420 PAGVELKIML SPEARNTAWV SFDGRKRQEI RHGDSISITT SCYPLPSICV RDPVSDWFES 430 440 LAQCLHWNVR KKQAHFEEEE EEEEEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional characterization of human NAD kinase." Lerner F., Niere M., Ludwig A., Ziegler M. Biochem. Biophys. Res. Commun. 288:69-74(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, VARIANT LYS-262. Tissue: Fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-262. Tissue: Lymph.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-48; SER-50 AND SER-64, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-48, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY090771 mRNA. Translation: AAM01195.1.
AK023114 mRNA. Translation: BAB14412.1.
AK290161 mRNA. Translation: BAF82850.1.
AL031282 Genomic DNA. Translation: CAA20354.1.
CH471183 Genomic DNA. Translation: EAW56151.1.
BC001709 mRNA. Translation: AAH01709.1.

IPI

IPI00794844.

RefSeq

NP_001185922.1. NM_001198993.1.
NP_001185923.1. NM_001198994.1.
NP_001185924.1. NM_001198995.1.
NP_075394.3. NM_023018.4.

UniGene

Hs.654792.
Hs.733075.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PFN	X-ray	2.70	A/B/C/D	68-426	[»]

ProteinModelPortal

O95544.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95544. 6 interactions.

MINT

MINT-3296423.

STRING

9606.ENSP00000341679.

PTM databases

PhosphoSite

O95544.

Proteomic databases

PaxDb

O95544.

PRIDE

O95544.

Protocols and materials databases

DNASU

65220.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000341426; ENSP00000341679; ENSG00000008130.
ENST00000341991; ENSP00000344340; ENSG00000008130.

GeneID

65220.

KEGG

hsa:65220.

UCSC

uc001aic.3. human.

Organism-specific databases

CTD

65220.

GeneCards

GC01M001682.

HGNC

HGNC:29831. NADK.

HPA

HPA048909.

MIM

611616. gene.

neXtProt

NX_O95544.

PharmGKB

PA142671298.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0061.

HOGENOM

HOG000176769.

HOVERGEN

HBG008249.

KO

K00858.

Enzyme and pathway databases

BRENDA

2.7.1.23. 2681.

Reactome

REACT_111217. Metabolism.

SABIO-RK

O95544.

Gene expression databases

ArrayExpress

O95544.

Bgee

O95544.

CleanEx

HS_NADK.

Genevestigator

O95544.

GermOnline

ENSG00000008130. Homo sapiens.

Family and domain databases

Gene3D

2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.

InterPro

IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK_prd.
[Graphical view]

PANTHER

PTHR20275. PTHR20275. 1 hit.

Pfam

PF01513. NAD_kinase. 1 hit.
[Graphical view]

SUPFAM

SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.

ProtoNet

Search...

Other

BindingDB

O95544.

ChEMBL

CHEMBL6177.

ChiTaRS

NADK. human.

EvolutionaryTrace

O95544.

GenomeRNAi

65220.

NextBio

67354.

SOURCE

Search...

Entry information

Entry name

NADK_HUMAN

Accession

Primary (citable) accession number: O95544
Secondary accession number(s): A8K296, Q9H931

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.