Reviewed, UniProtKB/Swiss-Prot O95544 (NADK_HUMAN)

Names and origin

Protein names

Recommended name:
    NAD kinase
    EC=2.7.1.23
Alternative name(s):
    Poly(P)/ATP NAD kinase

Gene names

Name:

NADK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + NAD+ = ADP + NADP+. Ref.1
Cofactor	Divalent metal cations. Ref.1
Tissue specificity	Widely expressed but not detected in skeletal muscle. Ref.1
Sequence similarities	Belongs to the NAD kinase family.
biophysicochemical properties	Kinetic parameters: KM=3.3 mM for ATP KM=0.54 mM for NAD Vmax=6.7 µmol/min/mg enzyme pH dependence: Optimum pH is 7.5. Temperature dependence: Optimum temperature is 55 degrees Celsius.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Metal-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP metabolic process Ref.1 Non-traceable author statement. Source: UniProtKB NAD metabolic process Inferred from Experiment. Source: Reactome phosphorylation Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	cytosol Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YWHAZ	P63104	1	EBI-743949,EBI-347088

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	NAD kinase		PRO_0000120713
Regions
	Compositional bias	326 – 333	8	Poly-Ala
	Compositional bias	437 – 445	9	Poly-Glu
Amino acid modifications
	Modified residue	46	1	Phosphoserine Ref.5
	Modified residue	48	1	Phosphoserine Ref.5
	Modified residue	50	1	Phosphoserine Ref.5
	Modified residue	64	1	Phosphoserine Ref.5
Natural variations
	Natural variant	262	1	N → K: dbSNP rs4751. Ref.1 Ref.4		VAR_034119
Experimental info
	Sequence conflict	445	1	E → EE in BAB14412. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O95544-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 48CE7AF05EDD7E8B	FASTA	446	49,228
	10 20 30 40 50 60 MEMEQEKMTM NKELSPDAAA YCCSACHGDE TWSYNHPIRG RAKSRSLSAS PALGSTKEFR 70 80 90 100 110 120 RTRSLHGPCP VTTFGPKACV LQNPQTIMHI QDPASQRLTW NKSPKSVLVI KKMRDASLLQ 130 140 150 160 170 180 PFKELCTHLM EENMIVYVEK KVLEDPAIAS DESFGAVKKK FCTFREDYDD ISNQIDFIIC 190 200 210 220 230 240 LGGDGTLLYA SSLFQGSVPP VMAFHLGSLG FLTPFSFENF QSQVTQVIEG NAAVVLRSRL 250 260 270 280 290 300 KVRVVKELRG KKTAVHNGLG ENGSQAAGLD MDVGKQAMQY QVLNEVVIDR GPSSYLSNVD 310 320 330 340 350 360 VYLDGHLITT VQGDGVIVST PTGSTAYAAA AGASMIHPNV PAIMITPICP HSLSFRPIVV 370 380 390 400 410 420 PAGVELKIML SPEARNTAWV SFDGRKRQEI RHGDSISITT SCYPLPSICV RDPVSDWFES 430 440 LAQCLHWNVR KKQAHFEEEE EEEEEG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional characterization of human NAD kinase." Lerner F., Niere M., Ludwig A., Ziegler M. Biochem. Biophys. Res. Commun. 288:69-74(2001) [PubMed: 11594753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, VARIANT LYS-262. Tissue: Fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-262. Tissue: Lymph.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; SER-48; SER-50 AND SER-64, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY090771 mRNA. Translation: AAM01195.1. AK023114 mRNA. Translation: BAB14412.1. AL031282 Genomic DNA. Translation: CAA20354.1. BC001709 mRNA. Translation: AAH01709.1.
IPI	IPI00794844.
RefSeq	NP_075394.3.
UniGene	Hs.654792
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95544. 7 interactions.
PTM databases
PhosphoSite	O95544.
Genome annotation databases
Ensembl	ENSG00000008130. Homo sapiens. [Contig view]
GeneID	65220.
KEGG	hsa:65220.
Organism-specific databases
GeneCards	GC01M001715.
H-InvDB	HIX0000040.
HGNC	HGNC:29831. NADK.
MIM	611616. gene.
PharmGKB	PA142671298.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O95544.
Enzyme and pathway databases
BRENDA	2.7.1.23. 247.
Reactome	REACT_11193. Metabolism of vitamins and cofactors.
Gene expression databases
ArrayExpress	O95544.
Bgee	O95544.
CleanEx	HS_NADK.
GermOnline	ENSG00000008130. Homo sapiens.
Family and domain databases
InterPro	IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. ATP_NADK. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	67354.
SOURCE	Search...

Entry information

Entry name

NADK_HUMAN

Accession

Primary (citable) accession number: O95544
Secondary accession number(s): Q9H931

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)