O95497 (VNN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantetheinase
EC=3.5.1.92

Alternative name(s):
Pantetheine hydrolase
Tiff66
Vascular non-inflammatory molecule 1
Short name=Vanin-1

Gene names

Name:

VNN1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Ref.7 Ref.8
Catalytic activity	(R)-pantetheine + H2O = (R)-pantothenate + 2-aminoethanethiol.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential.
Tissue specificity	Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin. Ref.11
Induction	By Th17/Th1 type cytokines, but not by Th2-type. Ref.11
Sequence similarities	Belongs to the CN hydrolase family. BTD/VNN subfamily. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acute inflammatory response Inferred from sequence or structural similarity. Source: BHF-UCL anti-apoptosis Inferred from sequence or structural similarity. Source: BHF-UCL cell-cell adhesion Inferred from sequence or structural similarity Ref.8. Source: BHF-UCL cellular component movement Traceable author statement. Source: ProtInc chronic inflammatory response Inferred from sequence or structural similarity. Source: BHF-UCL innate immune response Inferred from sequence or structural similarity. Source: BHF-UCL pantothenate metabolic process Inferred from direct assay Ref.8. Source: BHF-UCL positive regulation of T cell differentiation in thymus Inferred from sequence or structural similarity Ref.8. Source: BHF-UCL response to oxidative stress Traceable author statement. Source: BHF-UCL
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Traceable author statement. Source: BHF-UCL plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GPI anchor binding Traceable author statement. Source: BHF-UCL pantetheine hydrolase activity Inferred from direct assay Ref.8. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 491	470	Pantetheinase		PRO_0000019712
	Propeptide	492 – 513	22	Removed in mature form Potential		PRO_0000019713
Regions
	Domain	25 – 328	304	CN hydrolase
Amino acid modifications
	Lipidation	491	1	GPI-anchor amidated glycine Potential
	Glycosylation	38	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	130	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	283	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	315	1	N-linked (GlcNAc...) Ref.10
	Glycosylation	353	1	N-linked (GlcNAc...) Ref.9 Ref.10 Ref.12
Natural variations
	Natural variant	26	1	T → I. Ref.1 Ref.2 Ref.3 Ref.4 Corresponds to variant rs2294757 [ dbSNP | Ensembl ].		VAR_023529
	Natural variant	63	1	A → T. Ref.4		VAR_023967
	Natural variant	131	1	N → S. Ref.4 Ref.6 Corresponds to variant rs2272996 [ dbSNP | Ensembl ].		VAR_023968
	Natural variant	136	1	V → L. Ref.4 Corresponds to variant rs45610032 [ dbSNP | Ensembl ].		VAR_023969
	Natural variant	146	1	D → N. Ref.4		VAR_023970
	Natural variant	296	1	E → D. Ref.4 Corresponds to variant rs45523444 [ dbSNP | Ensembl ].		VAR_023971
	Natural variant	325	1	A → E. Ref.4 Corresponds to variant rs34535050 [ dbSNP | Ensembl ].		VAR_023972
	Natural variant	336	1	T → A. Ref.4 Corresponds to variant rs45562238 [ dbSNP | Ensembl ].		VAR_023973
	Natural variant	373	1	I → T. Ref.4 Corresponds to variant rs35938565 [ dbSNP | Ensembl ].		VAR_023974
Experimental info
	Sequence conflict	101	1	D → N in AAH96268. Ref.6
	Sequence conflict	113	1	N → I in AAY88742. Ref.4
	Sequence conflict	423	1	E → D in AAF21453. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O95497 [UniParc]. Last modified September 27, 2005. Version 2. Checksum: 018D2417C12E403F	FASTA	513	57,012
	10 20 30 40 50 60 MTTQLPAYVA ILLFYVSRAS CQDTFTAAVY EHAAILPNAT LTPVSREEAL ALMNRNLDIL 70 80 90 100 110 120 EGAITSAADQ GAHIIVTPED AIYGWNFNRD SLYPYLEDIP DPEVNWIPCN NRNRFGQTPV 130 140 150 160 170 180 QERLSCLAKN NSIYVVANIG DKKPCDTSDP QCPPDGRYQY NTDVVFDSQG KLVARYHKQN 190 200 210 220 230 240 LFMGENQFNV PKEPEIVTFN TTFGSFGIFT CFDILFHDPA VTLVKDFHVD TIVFPTAWMN 250 260 270 280 290 300 VLPHLSAVEF HSAWAMGMRV NFLASNIHYP SKKMTGSGIY APNSSRAFHY DMKTEEGKLL 310 320 330 340 350 360 LSQLDSHPSH SAVVNWTSYA SSIEALSSGN KEFKGTVFFD EFTFVKLTGV AGNYTVCQKD 370 380 390 400 410 420 LCCHLSYKMS ENIPNEVYAL GAFDGLHTVE GRYYLQICTL LKCKTTNLNT CGDSAETAST 430 440 450 460 470 480 RFEMFSLSGT FGTQYVFPEV LLSENQLAPG EFQVSTDGRL FSLKPTSGPV LTVTLFGRLY 490 500 510 EKDWASNASS GLTAQARIIM LIVIAPIVCS LSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two human genes related to murine vanin-1 are located on the long arm of human chromosome 6." Galland F., Malergue F., Bazin H., Mattei M.-G., Aurrand-Lions M., Theillet C., Naquet P. Genomics 53:203-213(1998) [PubMed: 9790769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-26. Tissue: Liver.
[2]	"Human Tiff66." Prehn S., Friedrichson T., Henske A., Boehm S., Hartmann E., Kurzchalia T.V. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-26.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-26. Tissue: Umbilical cord blood.
[4]	NIEHS SNPs program Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-26; THR-63; SER-131; LEU-136; ASN-146; ASP-296; GLU-325; ALA-336 AND THR-373.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-131.
[7]	"Is pantetheinase the actual identity of mouse and human vanin-1 proteins?" Maras B., Barra D., Dupre S., Pitari G. FEBS Lett. 461:149-152(1999) [PubMed: 10567687] [Abstract] Cited for: FUNCTION.
[8]	"Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes." Martin F., Malergue F., Pitari G., Philippe J.M., Philips S., Chabret C., Granjeaud S., Mattei M.G., Mungall A.J., Naquet P., Galland F. Immunogenetics 53:296-306(2001) [PubMed: 11491533] [Abstract] Cited for: FUNCTION.
[9]	"A proteomic analysis of human bile." Kristiansen T.Z., Bunkenborg J., Gronborg M., Molina H., Thuluvath P.J., Argani P., Goggins M.G., Maitra A., Pandey A. Mol. Cell. Proteomics 3:715-728(2004) [PubMed: 15084671] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-38 AND ASN-353, MASS SPECTROMETRY. Tissue: Bile.
[10]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-130; ASN-283; ASN-315 AND ASN-353, MASS SPECTROMETRY. Tissue: Plasma.
[11]	"Expression of the vanin gene family in normal and inflamed human skin: induction by proinflammatory cytokines." Jansen P.A.M., Kamsteeg M., Rodijk-Olthuis D., van Vlijmen-Willems I.M.J.J., de Jongh G.J., Bergers M., Tjabringa G.S., Zeeuwen P.L.J.M., Schalkwijk J. J. Invest. Dermatol. 129:2167-2174(2009) [PubMed: 19322213] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION BY CYTOKINES.
[12]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-353, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132099 mRNA. Translation: CAA10568.1. U39664 mRNA. Translation: AAF21453.1. AK290425 mRNA. Translation: BAF83114.1. DQ100297 Genomic DNA. Translation: AAY88742.1. AL032821 Genomic DNA. Translation: CAB40075.1. BC096265 mRNA. Translation: AAH96265.1. BC096266 mRNA. Translation: AAH96266.1. BC096267 mRNA. Translation: AAH96267.1. BC096268 mRNA. Translation: AAH96268.1.
IPI	IPI00030871.
RefSeq	NP_004657.2. NM_004666.2.
UniGene	Hs.12114. Hs.720659.
3D structure databases
ProteinModelPortal	O95497.
ModBase	Search...
Protein-protein interaction databases
STRING	O95497.
PTM databases
PhosphoSite	O95497.
Proteomic databases
PeptideAtlas	O95497.
PRIDE	O95497.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367928; ENSP00000356905; ENSG00000112299.
GeneID	8876.
KEGG	hsa:8876.
UCSC	uc003qdo.1. human.
Organism-specific databases
CTD	8876.
GeneCards	GC06M133001.
H-InvDB	HIX0022602.
HGNC	HGNC:12705. VNN1.
MIM	603570. gene.
neXtProt	NX_O95497.
PharmGKB	PA37321.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13191.
GeneTree	ENSGT00390000013823.
HOVERGEN	HBG003996.
InParanoid	O95497.
OMA	GNQEFKG.
OrthoDB	EOG4JHCFG.
PhylomeDB	O95497.
Gene expression databases
ArrayExpress	O95497.
Bgee	O95497.
CleanEx	HS_VNN1.
Genevestigator	O95497.
GermOnline	ENSG00000112299. Homo sapiens.
Family and domain databases
InterPro	IPR012101. Biotinidase_euk. IPR003010. Ntlse/CNhydtse. [Graphical view]
Gene3D	G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
KO	K08069.
PANTHER	PTHR10609. Biotinidase_euk. 1 hit.
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
PIRSF	PIRSF011861. Biotinidase. 1 hit.
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	33325.
SOURCE	Search...

Entry information

Entry name

VNN1_HUMAN

Accession

Primary (citable) accession number: O95497
Secondary accession number(s): A8K310 , Q4JFW6, Q4VAS7, Q4VAS8, Q4VAS9, Q9UF16, Q9UJF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.