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O95490 (LPHN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Latrophilin-2
Alternative name(s):
Calcium-independent alpha-latrotoxin receptor 2
Short name=CIRL-2
Latrophilin homolog 1
Lectomedin-1
Gene names
Name:LPHN2
Synonyms:KIAA0786, LEC1, LPHH1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length1459 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor propably implicated in the regulation of exocytosis By similarity.

Subunit structure

Forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85) By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed very widely in all normal tissues tested. Expression is variable in tumor cell lines, apparently elevated in some lines and absent or markedly reduced in others. Ref.1

Post-translational modification

Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 1 GPS domain.

Contains 1 olfactomedin-like domain.

Contains 1 SUEL-type lectin domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95490-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95490-2)

Also known as: Lectomedin-1 gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     595-607: Missing.
     1167-1209: Missing.
Isoform 3 (identifier: O95490-3)

Also known as: Lectomedin-1 beta;

The sequence of this isoform differs from the canonical sequence as follows:
     595-607: Missing.
     1134-1136: SRI → DIH
     1137-1459: Missing.
Isoform 4 (identifier: O95490-4)

Also known as: Lectomedin-1 alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     595-607: Missing.
     1167-1209: Missing.
     1210-1233: HSLNNARDTSAMDTLPLNGNFNNS → LTSHGLRAHLQDLYHLELLLGQIA
     1234-1459: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 14591434Latrophilin-2
PRO_0000012910

Regions

Topological domain39 – 851813Extracellular Potential
Transmembrane852 – 87221Helical; Name=1; Potential
Topological domain873 – 8808Cytoplasmic Potential
Transmembrane881 – 90121Helical; Name=2; Potential
Topological domain902 – 9076Extracellular Potential
Transmembrane908 – 92821Helical; Name=3; Potential
Topological domain929 – 95224Cytoplasmic Potential
Transmembrane953 – 97321Helical; Name=4; Potential
Topological domain974 – 99017Extracellular Potential
Transmembrane991 – 101121Helical; Name=5; Potential
Topological domain1012 – 103726Cytoplasmic Potential
Transmembrane1038 – 105821Helical; Name=6; Potential
Topological domain1059 – 10624Extracellular Potential
Transmembrane1063 – 108321Helical; Name=7; Potential
Topological domain1084 – 1459376Cytoplasmic Potential
Domain41 – 13090SUEL-type lectin
Domain135 – 394260Olfactomedin-like
Domain784 – 83653GPS

Sites

Site824 – 8252Cleavage By similarity

Amino acid modifications

Modified residue13811Phosphoserine By similarity
Modified residue13871Phosphoserine By similarity
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation5201N-linked (GlcNAc...) Potential
Glycosylation6291N-linked (GlcNAc...) Ref.7
Glycosylation7311N-linked (GlcNAc...) Potential
Glycosylation7441N-linked (GlcNAc...) Potential
Glycosylation7871N-linked (GlcNAc...) Potential
Glycosylation7921N-linked (GlcNAc...) Potential
Glycosylation8131N-linked (GlcNAc...) Potential
Disulfide bond136 ↔ 318 By similarity

Natural variations

Alternative sequence595 – 60713Missing in isoform 2, isoform 3 and isoform 4.
VSP_010104
Alternative sequence1134 – 11363SRI → DIH in isoform 3.
VSP_010105
Alternative sequence1137 – 1459323Missing in isoform 3.
VSP_010106
Alternative sequence1167 – 120943Missing in isoform 2 and isoform 4.
VSP_010107
Alternative sequence1210 – 123324HSLNN…NFNNS → LTSHGLRAHLQDLYHLELLL GQIA in isoform 4.
VSP_010108
Alternative sequence1234 – 1459226Missing in isoform 4.
VSP_010109

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 26, 2004. Version 2.
Checksum: 30707B7C3C069029

FASTA1,459163,349
        10         20         30         40         50         60 
MVSSGCRMRS LWFIIVISFL PNTEGFSRAA LPFGLVRREL SCEGYSIDLR CPGSDVIMIE 

        70         80         90        100        110        120 
SANYGRTDDK ICDADPFQME NTDCYLPDAF KIMTQRCNNR TQCIVVTGSD VFPDPCPGTY 

       130        140        150        160        170        180 
KYLEVQYECV PYIFVCPGTL KAIVDSPCIY EAEQKAGAWC KDPLQAADKI YFMPWTPYRT 

       190        200        210        220        230        240 
DTLIEYASLE DFQNSRQTTT YKLPNRVDGT GFVVYDGAVF FNKERTRNIV KFDLRTRIKS 

       250        260        270        280        290        300 
GEAIINYANY HDTSPYRWGG KTDIDLAVDE NGLWVIYATE QNNGMIVISQ LNPYTLRFEA 

       310        320        330        340        350        360 
TWETVYDKRA ASNAFMICGV LYVVRSVYQD NESETGKNSI DYIYNTRLNR GEYVDVPFPN 

       370        380        390        400        410        420 
QYQYIAAVDY NPRDNQLYVW NNNFILRYSL EFGPPDPAQV PTTAVTITSS AELFKTIIST 

       430        440        450        460        470        480 
TSTTSQKGPM STTVAGSQEG SKGTKPPPAV STTKIPPITN IFPLPERFCE ALDSKGIKWP 

       490        500        510        520        530        540 
QTQRGMMVER PCPKGTRGTA SYLCMISTGT WNPKGPDLSN CTSHWVNQLA QKIRSGENAA 

       550        560        570        580        590        600 
SLANELAKHT KGPVFAGDVS SSVRLMEQLV DILDAQLQEL KPSEKDSAGR SYNKLQKREK 

       610        620        630        640        650        660 
TCRAYLKAIV DTVDNLLRPE ALESWKHMNS SEQAHTATML LDTLEEGAFV LADNLLEPTR 

       670        680        690        700        710        720 
VSMPTENIVL EVAVLSTEGQ IQDFKFPLGI KGAGSSIQLS ANTVKQNSRN GLAKLVFIIY 

       730        740        750        760        770        780 
RSLGQFLSTE NATIKLGADF IGRNSTIAVN SHVISVSINK ESSRVYLTDP VLFTLPHIDP 

       790        800        810        820        830        840 
DNYFNANCSF WNYSERTMMG YWSTQGCKLV DTNKTRTTCA CSHLTNFAIL MAHREIAYKD 

       850        860        870        880        890        900 
GVHELLLTVI TWVGIVISLV CLAICIFTFC FFRGLQSDRN TIHKNLCINL FIAEFIFLIG 

       910        920        930        940        950        960 
IDKTKYAIAC PIFAGLLHFF FLAAFAWMCL EGVQLYLMLV EVFESEYSRK KYYYVAGYLF 

       970        980        990       1000       1010       1020 
PATVVGVSAA IDYKSYGTEK ACWLHVDNYF IWSFIGPVTF IILLNIIFLV ITLCKMVKHS 

      1030       1040       1050       1060       1070       1080 
NTLKPDSSRL ENIKSWVLGA FALLCLLGLT WSFGLLFINE ETIVMAYLFT IFNAFQGVFI 

      1090       1100       1110       1120       1130       1140 
FIFHCALQKK VRKEYGKCFR HSYCCGGLPT ESPHSSVKAS TTRTSARYSS GTQSRIRRMW 

      1150       1160       1170       1180       1190       1200 
NDTVRKQSES SFISGDINST STLNQGMTGN YLLTNPLLRP HGTNNPYNTL LAETVVCNAP 

      1210       1220       1230       1240       1250       1260 
SAPVFNSPGH SLNNARDTSA MDTLPLNGNF NNSYSLHKGD YNDSVQVVDC GLSLNDTAFE 

      1270       1280       1290       1300       1310       1320 
KMIISELVHN NLRGSSKTHN LELTLPVKPV IGGSSSEDDA IVADASSLMH SDNPGLELHH 

      1330       1340       1350       1360       1370       1380 
KELEAPLIPQ RTHSLLYQPQ KKVKSEGTDS YVSQLTAEAE DHLQSPNRDS LYTSMPNLRD 

      1390       1400       1410       1420       1430       1440 
SPYPESSPDM EEDLSPSRRS ENEDIYYKSM PNLGAGHQLQ MCYQISRGNS DGYIIPINKE 

      1450 
GCIPEGDVRE GQMQLVTSL

« Hide

Isoform 2 (Lectomedin-1 gamma) [UniParc].

Checksum: CE43AB82916D0D35
Show »

FASTA1,403157,178
Isoform 3 (Lectomedin-1 beta) [UniParc].

Checksum: 21DB98B51EBDF4EC
Show »

FASTA1,123126,163
Isoform 4 (Lectomedin-1 alpha) [UniParc].

Checksum: 6D099178841E1E3A
Show »

FASTA1,177132,245

References

« Hide 'large scale' references
[1]"Isolation and characterisation of a human homologue of the latrophilin gene from a region of 1p31.1 implicated in breast cancer."
White G.R.M., Varley J.M., Heighway J.
Oncogene 17:3513-3519(1998) [PubMed: 10030676] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
[2]"Genomic structure and expression profile of LPHH1, a 7TM gene variably expressed in breast cancer cell lines."
White G.R.M., Varley J.M., Heighway J.
Biochim. Biophys. Acta 1491:75-92(2000) [PubMed: 10760572] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Hayflick J.S.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4).
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 439-1459 (ISOFORM 1).
Tissue: Brain.
[7]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-629, MASS SPECTROMETRY.
Tissue: Liver.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ131581 mRNA. Translation: CAA10458.1.
AJ244492 expand/collapse EMBL AC list , AJ244493, AJ244494, AJ244496, AJ244497, AJ244498, AJ244499, AJ244501, AJ244502, AJ244503, AJ244504, AJ244505, AJ244506, AJ244507, AJ244510, AJ244511, AJ244512, AJ244516 Genomic DNA. Translation: CAB60229.1.
AF104938 mRNA. Translation: AAD54676.1.
CH471059 Genomic DNA. Translation: EAX06330.1.
CH471059 Genomic DNA. Translation: EAX06333.1.
CH471059 Genomic DNA. Translation: EAX06336.1.
AF104266 mRNA. Translation: AAD54675.1.
AF104939 mRNA. Translation: AAD54677.1.
AL359705, AC113949, AL157903 Genomic DNA. Translation: CAH71340.1.
AL359705, AC113949, AL157903 Genomic DNA. Translation: CAH71341.1.
AL359705, AC113949, AL157903 Genomic DNA. Translation: CAH71342.1.
AL359705, AC113949, AL157903 Genomic DNA. Translation: CAH71347.1.
AL157903, AC113949, AL359705 Genomic DNA. Translation: CAI22397.1.
AL157903, AC113949, AL359705 Genomic DNA. Translation: CAI22398.1.
AL157903, AC113949, AL359705 Genomic DNA. Translation: CAI22399.1.
AL157903, AC113949, AL359705 Genomic DNA. Translation: CAI22401.1.
AB018329 mRNA. Translation: BAA34506.1.
IPIIPI00017562.
IPI00164345.
IPI00410232.
IPI00410234.
RefSeqNP_036434.1. NM_012302.2.
UniGeneHs.24212.
Hs.649282.

3D structure databases

ProteinModelPortalO95490.
SMRO95490. Positions 30-132, 465-522.
ModBaseSearch...

Protein-protein interaction databases

IntActO95490. 2 interactions.
MINTMINT-143559.
STRINGO95490.

Protein family/group databases

MEROPSS63.012.
GPCRDBSearch...

PTM databases

PhosphoSiteO95490.

Proteomic databases

PRIDEO95490.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000335786; ENSP00000337306; ENSG00000117114.
ENST00000370728; ENSP00000359763; ENSG00000117114.
GeneID23266.
KEGGhsa:23266.
UCSCuc001dit.2. human.
uc001div.1. human.
uc009wcd.1. human.

Organism-specific databases

CTD23266.
GeneCardsGC01P081771.
H-InvDBHIX0000727.
HGNCHGNC:18582. LPHN2.
HPAHPA013573.
MIM607018. gene.
neXtProtNX_O95490.
PharmGKBPA38589.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG05698.
HOVERGENHBG052337.
PhylomeDBO95490.

Gene expression databases

ArrayExpressO95490.
BgeeO95490.
CleanExHS_LPHN2.
GenevestigatorO95490.
GermOnlineENSG00000117114. Homo sapiens.

Family and domain databases

InterProIPR022624. DUF3497.
IPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003924. GPCR_2_latrophilin.
IPR003334. GPCR_2_latrophilin_rcpt_C.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS_dom.
IPR000922. Lectin_gal-bd_dom.
IPR003112. Olfac-like.
[Graphical view]
KOK04593.
PfamPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF02140. Gal_Lectin. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF02354. Latrophilin. 1 hit.
PF02191. OLF. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01444. LATROPHILIN.
SMARTSM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00284. OLF. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. False negative.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS51132. OLF. 1 hit.
PS50228. SUEL_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio45018.
SOURCESearch...

Entry information

Entry nameLPHN2_HUMAN
AccessionPrimary (citable) accession number: O95490
Secondary accession number(s): B1ALT8 expand/collapse secondary AC list , B1ALT9, B1ALU0, O94882, Q5VX76, Q9UKY5, Q9UKY6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents