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O95487 (SC24B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein Sec24B
Alternative name(s):
SEC24-related protein B
Gene names
Name:SEC24B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1268 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

Subunit structure

COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and SAR1. SEC24B is capable of forming heterodimers with SEC24A. Interacts with RNF139. Interacts with TMED2 and TMED10. Ref.7 Ref.9

Subcellular location

Cytoplasm. Cytoplasmperinuclear region. Endoplasmic reticulum membrane Potential. Golgi apparatus membrane Potential. Golgi apparatus membrane By similarity.

Tissue specificity

Expressed in fibroblasts, hepatocytes, and lymphocytes.

Sequence similarities

Belongs to the SEC23/SEC24 family. SEC24 subfamily.

Sequence caution

The sequence CAA10335.1 differs from that shown. Reason: Frameshift at positions 459 and 483.

Ontologies

Keywords
   Biological processER-Golgi transport
Protein transport
Transport
   Cellular componentCytoplasm
Endoplasmic reticulum
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCOPII vesicle coating

Traceable author statement. Source: Reactome

ER to Golgi vesicle-mediated transport

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class II

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cellular protein metabolic process

Traceable author statement. Source: Reactome

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

membrane organization

Traceable author statement. Source: Reactome

post-translational protein modification

Traceable author statement. Source: Reactome

protein N-linked glycosylation via asparagine

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

vesicle-mediated transport

Traceable author statement Ref.1. Source: ProtInc

   Cellular_componentCOPII vesicle coat

Inferred from electronic annotation. Source: InterPro

ER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Traceable author statement. Source: Reactome

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

membrane

Traceable author statement Ref.1. Source: ProtInc

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

transporter activity

Traceable author statement Ref.1. Source: ProtInc

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95487-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95487-2)

The sequence of this isoform differs from the canonical sequence as follows:
     354-388: Missing.
Isoform 3 (identifier: O95487-3)

The sequence of this isoform differs from the canonical sequence as follows:
     44-44: N → NETGFHHVAQASLELLDPSNLPASASQIAGST
     496-496: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 12681267Protein transport protein Sec24B
PRO_0000205155

Regions

Region605 – 62925Zinc finger-like
Compositional bias379 – 3879Poly-Glu

Amino acid modifications

Modified residue21N-acetylserine Ref.6
Modified residue12241Phosphoserine Ref.4

Natural variations

Alternative sequence441N → NETGFHHVAQASLELLDPSN LPASASQIAGST in isoform 3.
VSP_054432
Alternative sequence354 – 38835Missing in isoform 2.
VSP_035987
Alternative sequence4961Missing in isoform 3.
VSP_054433
Natural variant4561A → G.
Corresponds to variant rs35705351 [ dbSNP | Ensembl ].
VAR_047934

Experimental info

Sequence conflict231A → S in CAA10335. Ref.1

Secondary structure

................................................................................................................................... 1268
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 0E08B982D0982F84

FASTA1,268137,418
        10         20         30         40         50         60 
MSAPAGSSHP AASARIPPKF GGAAVSGAAA PAGPGAGPAP HQQNGPAQNQ MQVPSGYGLH 

        70         80         90        100        110        120 
HQNYIAPSGH YSQGPGKMTS LPLDTQCGDY YSALYTVPTQ NVTPNTVNQQ PGAQQLYSRG 

       130        140        150        160        170        180 
PPAPHIVGST LGSFQGAASS ASHLHTSASQ PYSSFVNHYN SPAMYSASSS VASQGFPSTC 

       190        200        210        220        230        240 
GHYAMSTVSN AAYPSVSYPS LPAGDTYGQM FTSQNAPTVR PVKDNSFSGQ NTAISHPSPL 

       250        260        270        280        290        300 
PPLPSQQHHQ QQSLSGYSTL TWSSPGLPST QDNLIRNHTG SLAVANNNPT ITVADSLSCP 

       310        320        330        340        350        360 
VMQNVQPPKS SPVVSTVLSG SSGSSSTRTP PTANHPVEPV TSVTQPSELL QQKGVQYGEY 

       370        380        390        400        410        420 
VNNQASSAPT PLSSTSDDEE EEEEDEEAGV DSSSTTSSAS PMPNSYDALE GGSYPDMLSS 

       430        440        450        460        470        480 
SASSPAPDPA PEPDPASAPA PASAPAPVVP QPSKMAKPFG YGYPTLQPGY QNATAPLISG 

       490        500        510        520        530        540 
VQPSNPVYSG FQQYPQQYPG VNQLSSSIGG LSLQSSPQPE SLRPVNLTQE RNILPMTPVW 

       550        560        570        580        590        600 
APVPNLNADL KKLNCSPDSF RCTLTNIPQT QALLNKAKLP LGLLLHPFRD LTQLPVITSN 

       610        620        630        640        650        660 
TIVRCRSCRT YINPFVSFID QRRWKCNLCY RVNDVPEEFM YNPLTRSYGE PHKRPEVQNS 

       670        680        690        700        710        720 
TVEFIASSDY MLRPPQPAVY LFVLDVSHNA VEAGYLTILC QSLLENLDKL PGDSRTRIGF 

       730        740        750        760        770        780 
MTFDSTIHFY NLQEGLSQPQ MLIVSDIDDV FLPTPDSLLV NLYESKELIK DLLNALPNMF 

       790        800        810        820        830        840 
TNTRETHSAL GPALQAAFKL MSPTGGRVSV FQTQLPSLGA GLLQSREDPN QRSSTKVVQH 

       850        860        870        880        890        900 
LGPATDFYKK LALDCSGQQT AVDLFLLSSQ YSDLASLACM SKYSAGCIYY YPSFHYTHNP 

       910        920        930        940        950        960 
SQAEKLQKDL KRYLTRKIGF EAVMRIRCTK GLSMHTFHGN FFVRSTDLLS LANINPDAGF 

       970        980        990       1000       1010       1020 
AVQLSIEESL TDTSLVCFQT ALLYTSSKGE RRIRVHTLCL PVVSSLADVY AGVDVQAAIC 

      1030       1040       1050       1060       1070       1080 
LLANMAVDRS VSSSLSDARD ALVNAVVDSL SAYGSTVSNL QHSALMAPSS LKLFPLYVLA 

      1090       1100       1110       1120       1130       1140 
LLKQKAFRTG TSTRLDDRVY AMCQIKSQPL VHLMKMIHPN LYRIDRLTDE GAVHVNDRIV 

      1150       1160       1170       1180       1190       1200 
PQPPLQKLSA EKLTREGAFL MDCGSVFYIW VGKGCDNNFI EDVLGYTNFA SIPQKMTHLP 

      1210       1220       1230       1240       1250       1260 
ELDTLSSERA RSFITWLRDS RPLSPILHIV KDESPAKAEF FQHLIEDRTE AAFSYYEFLL 


HVQQQICK 

« Hide

Isoform 2 [UniParc].

Checksum: 95EEBB976F613B6B
Show »

FASTA1,233133,629
Isoform 3 [UniParc].

Checksum: 3B649E12AEF0F17C
Show »

FASTA1,298140,421

References

« Hide 'large scale' references
[1]"Sec24 proteins and sorting at the endoplasmic reticulum."
Pagano A., Letourneur F., Garcia-Estefania D., Carpentier J.-L., Orci L., Paccaud J.-P.
J. Biol. Chem. 274:7833-7840(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: B-cell.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Brain and Eye.
[4]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1224, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[7]"The sterol-sensing endoplasmic reticulum (ER) membrane protein TRC8 hampers ER to Golgi transport of sterol regulatory element-binding protein-2 (SREBP-2)/SREBP cleavage-activated protein and reduces SREBP-2 cleavage."
Irisawa M., Inoue J., Ozawa N., Mori K., Sato R.
J. Biol. Chem. 284:28995-29004(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RNF139.
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"Selective export of human GPI-anchored proteins from the endoplasmic reticulum."
Bonnon C., Wendeler M.W., Paccaud J.P., Hauri H.P.
J. Cell Sci. 123:1705-1715(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TMED2 AND TMED10.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ131245 mRNA. Translation: CAA10335.1. Frameshift.
AC105314 Genomic DNA. No translation available.
AC138782 Genomic DNA. No translation available.
BC040137 mRNA. Translation: AAH40137.1.
BC117135 mRNA. Translation: AAI17136.1.
BC143268 mRNA. Translation: AAI43269.1.
BC143276 mRNA. Translation: AAI43277.1.
CCDSCCDS43260.1. [O95487-2]
CCDS47124.1. [O95487-1]
RefSeqNP_001036199.1. NM_001042734.1. [O95487-2]
NP_006314.2. NM_006323.2. [O95487-1]
XP_005262746.1. XM_005262689.1. [O95487-3]
UniGeneHs.292472.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3EH1X-ray1.80A518-1268[»]
ProteinModelPortalO95487.
SMRO95487. Positions 518-1268.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115696. 12 interactions.
IntActO95487. 10 interactions.
MINTMINT-6772353.
STRING9606.ENSP00000265175.

PTM databases

PhosphoSiteO95487.

Proteomic databases

MaxQBO95487.
PaxDbO95487.
PRIDEO95487.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265175; ENSP00000265175; ENSG00000138802. [O95487-1]
ENST00000399100; ENSP00000382051; ENSG00000138802. [O95487-2]
ENST00000504968; ENSP00000428564; ENSG00000138802.
GeneID10427.
KEGGhsa:10427.
UCSCuc003hzk.3. human. [O95487-1]
uc003hzl.3. human. [O95487-2]

Organism-specific databases

CTD10427.
GeneCardsGC04P110354.
HGNCHGNC:10704. SEC24B.
HPAHPA038181.
MIM607184. gene.
neXtProtNX_O95487.
PharmGKBPA35627.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5028.
HOGENOMHOG000196365.
HOVERGENHBG054850.
KOK14007.
PhylomeDBO95487.
TreeFamTF354244.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_11123. Membrane Trafficking.
REACT_17015. Metabolism of proteins.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressO95487.
BgeeO95487.
CleanExHS_SEC24B.
GenevestigatorO95487.

Family and domain databases

Gene3D3.40.20.10. 1 hit.
3.40.50.410. 1 hit.
InterProIPR029006. ADF-H/Gelsolin-like_dom.
IPR007123. Gelsolin-like_dom.
IPR006900. Sec23/24_helical_dom.
IPR006896. Sec23/24_trunk_dom.
IPR012990. Sec23_24_beta_S.
IPR002035. VWF_A.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]
PfamPF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
SSF81811. SSF81811. 1 hit.
SSF82919. SSF82919. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSEC24B. human.
EvolutionaryTraceO95487.
GeneWikiSEC24B.
GenomeRNAi10427.
NextBio35481162.
PROO95487.
SOURCESearch...

Entry information

Entry nameSC24B_HUMAN
AccessionPrimary (citable) accession number: O95487
Secondary accession number(s): B7ZKM8, B7ZKN4, Q0VG08
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM