Reviewed, UniProtKB/Swiss-Prot O95487 (SC24B_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein transport protein Sec24B
Alternative name(s):
    SEC24-related protein B

Gene names

Name:

SEC24B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
Subunit structure	COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Sec24B is capable of forming heterodimers with Sec24A.
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Endoplasmic reticulum membrane Potential. Golgi apparatus membrane Potential. Golgi apparatus membrane By similarity.
Tissue specificity	Expressed in fibroblasts, hepatocytes, and lymphocytes.
Sequence similarities	Belongs to the SEC23/SEC24 family. SEC24 subfamily.
Sequence caution	The sequence CAA10335.1 differs from that shown. Reason: Frameshift at positions 459 and 483.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from Experiment. Source: Reactome intracellular protein transport Inferred from electronic annotation. Source: InterPro membrane organization Inferred from Experiment. Source: Reactome
Cellular component	COPII vesicle coat Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct transporter activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PLCG1	P19174	1	EBI-81101,EBI-79387
SEC23A	Q15436	1	EBI-81101,EBI-81088

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95487-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95487-2)

The sequence of this isoform differs from the canonical sequence as follows:
     354-388: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1268

1268

Protein transport protein Sec24B

PRO_0000205155

Regions

Region

605 – 629

25

Zinc finger-like

Compositional bias

379 – 387

9

Poly-Glu

Amino acid modifications

Modified residue

117

1

Phosphotyrosine Ref.4

Modified residue

1100

1

Phosphotyrosine Ref.6

Modified residue

1224

1

Phosphoserine Ref.4 Ref.5

Natural variations

Alternative sequence

354 – 388

35

Missing in isoform 2.

VSP_035987

Natural variant

456

1

A → G: dbSNP rs35705351.

VAR_047934

Experimental info

Sequence conflict

23

1

A → S in CAA10335. Ref.1

Secondary structure

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1268

Helix Strand Turn

Details...

Helix

520 – 522

3

Beta strand

524 – 526

3

Turn

527 – 529

3

Helix

548 – 553

6

Turn

557 – 559

3

Beta strand

565 – 570

6

Helix

571 – 577

7

Turn

606 – 608

3

Beta strand

617 – 625

9

Turn

627 – 629

3

Beta strand

632 – 634

3

Helix

637 – 639

3

Helix

651 – 653

3

Helix

655 – 658

4

Beta strand

660 – 666

7

Helix

668 – 670

3

Beta strand

672 – 674

3

Beta strand

681 – 685

5

Helix

688 – 692

5

Helix

696 – 706

11

Turn

707 – 709

3

Beta strand

721 – 731

11

Beta strand

740 – 745

6

Helix

755 – 757

3

Beta strand

759 – 761

3

Turn

762 – 765

4

Helix

766 – 775

10

Helix

776 – 778

3

Helix

793 – 801

9

Beta strand

808 – 812

5

Helix

831 – 834

4

Beta strand

835 – 837

3

Helix

847 – 855

9

Beta strand

862 – 866

5

Helix

874 – 877

4

Helix

879 – 882

4

Beta strand

888 – 890

3

Turn

896 – 898

3

Helix

900 – 915

16

Beta strand

922 – 928

7

Beta strand

933 – 938

6

Beta strand

945 – 953

9

Beta strand

961 – 965

5

Beta strand

974 – 985

12

Beta strand

991 – 1003

13

Helix

1006 – 1010

5

Helix

1015 – 1032

18

Helix

1035 – 1052

18

Beta strand

1066 – 1068

3

Helix

1069 – 1071

3

Helix

1074 – 1082

9

Turn

1085 – 1087

3

Helix

1095 – 1107

13

Helix

1110 – 1117

8

Beta strand

1120 – 1123

4

Beta strand

1133 – 1135

3

Beta strand

1138 – 1140

3

Helix

1150 – 1152

3

Beta strand

1159 – 1162

4

Beta strand

1166 – 1171

6

Helix

1177 – 1182

6

Helix

1189 – 1191

3

Beta strand

1194 – 1196

3

Helix

1205 – 1219

15

Beta strand

1221 – 1223

3

Beta strand

1226 – 1236

11

Helix

1237 – 1241

5

Helix

1255 – 1267

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 0E08B982D0982F84	FASTA	1,268	137,418
	10 20 30 40 50 60 MSAPAGSSHP AASARIPPKF GGAAVSGAAA PAGPGAGPAP HQQNGPAQNQ MQVPSGYGLH 70 80 90 100 110 120 HQNYIAPSGH YSQGPGKMTS LPLDTQCGDY YSALYTVPTQ NVTPNTVNQQ PGAQQLYSRG 130 140 150 160 170 180 PPAPHIVGST LGSFQGAASS ASHLHTSASQ PYSSFVNHYN SPAMYSASSS VASQGFPSTC 190 200 210 220 230 240 GHYAMSTVSN AAYPSVSYPS LPAGDTYGQM FTSQNAPTVR PVKDNSFSGQ NTAISHPSPL 250 260 270 280 290 300 PPLPSQQHHQ QQSLSGYSTL TWSSPGLPST QDNLIRNHTG SLAVANNNPT ITVADSLSCP 310 320 330 340 350 360 VMQNVQPPKS SPVVSTVLSG SSGSSSTRTP PTANHPVEPV TSVTQPSELL QQKGVQYGEY 370 380 390 400 410 420 VNNQASSAPT PLSSTSDDEE EEEEDEEAGV DSSSTTSSAS PMPNSYDALE GGSYPDMLSS 430 440 450 460 470 480 SASSPAPDPA PEPDPASAPA PASAPAPVVP QPSKMAKPFG YGYPTLQPGY QNATAPLISG 490 500 510 520 530 540 VQPSNPVYSG FQQYPQQYPG VNQLSSSIGG LSLQSSPQPE SLRPVNLTQE RNILPMTPVW 550 560 570 580 590 600 APVPNLNADL KKLNCSPDSF RCTLTNIPQT QALLNKAKLP LGLLLHPFRD LTQLPVITSN 610 620 630 640 650 660 TIVRCRSCRT YINPFVSFID QRRWKCNLCY RVNDVPEEFM YNPLTRSYGE PHKRPEVQNS 670 680 690 700 710 720 TVEFIASSDY MLRPPQPAVY LFVLDVSHNA VEAGYLTILC QSLLENLDKL PGDSRTRIGF 730 740 750 760 770 780 MTFDSTIHFY NLQEGLSQPQ MLIVSDIDDV FLPTPDSLLV NLYESKELIK DLLNALPNMF 790 800 810 820 830 840 TNTRETHSAL GPALQAAFKL MSPTGGRVSV FQTQLPSLGA GLLQSREDPN QRSSTKVVQH 850 860 870 880 890 900 LGPATDFYKK LALDCSGQQT AVDLFLLSSQ YSDLASLACM SKYSAGCIYY YPSFHYTHNP 910 920 930 940 950 960 SQAEKLQKDL KRYLTRKIGF EAVMRIRCTK GLSMHTFHGN FFVRSTDLLS LANINPDAGF 970 980 990 1000 1010 1020 AVQLSIEESL TDTSLVCFQT ALLYTSSKGE RRIRVHTLCL PVVSSLADVY AGVDVQAAIC 1030 1040 1050 1060 1070 1080 LLANMAVDRS VSSSLSDARD ALVNAVVDSL SAYGSTVSNL QHSALMAPSS LKLFPLYVLA 1090 1100 1110 1120 1130 1140 LLKQKAFRTG TSTRLDDRVY AMCQIKSQPL VHLMKMIHPN LYRIDRLTDE GAVHVNDRIV 1150 1160 1170 1180 1190 1200 PQPPLQKLSA EKLTREGAFL MDCGSVFYIW VGKGCDNNFI EDVLGYTNFA SIPQKMTHLP 1210 1220 1230 1240 1250 1260 ELDTLSSERA RSFITWLRDS RPLSPILHIV KDESPAKAEF FQHLIEDRTE AAFSYYEFLL HVQQQICK
	Isoform 2. Checksum: 95EEBB976F613B6B	FASTA	1,233	133,629
	10 20 30 40 50 60 MSAPAGSSHP AASARIPPKF GGAAVSGAAA PAGPGAGPAP HQQNGPAQNQ MQVPSGYGLH 70 80 90 100 110 120 HQNYIAPSGH YSQGPGKMTS LPLDTQCGDY YSALYTVPTQ NVTPNTVNQQ PGAQQLYSRG 130 140 150 160 170 180 PPAPHIVGST LGSFQGAASS ASHLHTSASQ PYSSFVNHYN SPAMYSASSS VASQGFPSTC 190 200 210 220 230 240 GHYAMSTVSN AAYPSVSYPS LPAGDTYGQM FTSQNAPTVR PVKDNSFSGQ NTAISHPSPL 250 260 270 280 290 300 PPLPSQQHHQ QQSLSGYSTL TWSSPGLPST QDNLIRNHTG SLAVANNNPT ITVADSLSCP 310 320 330 340 350 360 VMQNVQPPKS SPVVSTVLSG SSGSSSTRTP PTANHPVEPV TSVTQPSELL QQKGVDSSST 370 380 390 400 410 420 TSSASPMPNS YDALEGGSYP DMLSSSASSP APDPAPEPDP ASAPAPASAP APVVPQPSKM 430 440 450 460 470 480 AKPFGYGYPT LQPGYQNATA PLISGVQPSN PVYSGFQQYP QQYPGVNQLS SSIGGLSLQS 490 500 510 520 530 540 SPQPESLRPV NLTQERNILP MTPVWAPVPN LNADLKKLNC SPDSFRCTLT NIPQTQALLN 550 560 570 580 590 600 KAKLPLGLLL HPFRDLTQLP VITSNTIVRC RSCRTYINPF VSFIDQRRWK CNLCYRVNDV 610 620 630 640 650 660 PEEFMYNPLT RSYGEPHKRP EVQNSTVEFI ASSDYMLRPP QPAVYLFVLD VSHNAVEAGY 670 680 690 700 710 720 LTILCQSLLE NLDKLPGDSR TRIGFMTFDS TIHFYNLQEG LSQPQMLIVS DIDDVFLPTP 730 740 750 760 770 780 DSLLVNLYES KELIKDLLNA LPNMFTNTRE THSALGPALQ AAFKLMSPTG GRVSVFQTQL 790 800 810 820 830 840 PSLGAGLLQS REDPNQRSST KVVQHLGPAT DFYKKLALDC SGQQTAVDLF LLSSQYSDLA 850 860 870 880 890 900 SLACMSKYSA GCIYYYPSFH YTHNPSQAEK LQKDLKRYLT RKIGFEAVMR IRCTKGLSMH 910 920 930 940 950 960 TFHGNFFVRS TDLLSLANIN PDAGFAVQLS IEESLTDTSL VCFQTALLYT SSKGERRIRV 970 980 990 1000 1010 1020 HTLCLPVVSS LADVYAGVDV QAAICLLANM AVDRSVSSSL SDARDALVNA VVDSLSAYGS 1030 1040 1050 1060 1070 1080 TVSNLQHSAL MAPSSLKLFP LYVLALLKQK AFRTGTSTRL DDRVYAMCQI KSQPLVHLMK 1090 1100 1110 1120 1130 1140 MIHPNLYRID RLTDEGAVHV NDRIVPQPPL QKLSAEKLTR EGAFLMDCGS VFYIWVGKGC 1150 1160 1170 1180 1190 1200 DNNFIEDVLG YTNFASIPQK MTHLPELDTL SSERARSFIT WLRDSRPLSP ILHIVKDESP 1210 1220 1230 AKAEFFQHLI EDRTEAAFSY YEFLLHVQQQ ICK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sec24 proteins and sorting at the endoplasmic reticulum." Pagano A., Letourneur F., Garcia-Estefania D., Carpentier J.-L., Orci L., Paccaud J.-P. J. Biol. Chem. 274:7833-7840(1999) [PubMed: 10075675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: B-cell.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." Tao W.A., Wollscheid B., O'Brien R., Eng J.K., Li X.-J., Bodenmiller B., Watts J.D., Hood L., Aebersold R. Nat. Methods 2:591-598(2005) [PubMed: 16094384] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-117 AND SER-1224, MASS SPECTROMETRY. Tissue: T-cell.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1224, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1100, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ131245 mRNA. Translation: CAA10335.1. Frameshift.
AC105314 Genomic DNA. No translation available.
AC138782 Genomic DNA. No translation available.
BC117135 mRNA. Translation: AAI17136.1.
BC143276 mRNA. Translation: AAI43277.1.

IPI

IPI00785208.
IPI00946469.

RefSeq

NP_001036199.1.
NP_006314.2.

UniGene

Hs.292472

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EH1	X-ray	1.80	A	518-1268	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O95487. 2 interactions.

STRING

O95487.

PTM databases

PhosphoSite

O95487.

Proteomic databases

PRIDE

O95487.

Genome annotation databases

Ensembl

ENST00000265175; ENSP00000265175; ENSG00000138802; Homo sapiens. [Genome view]
ENST00000399100; ENSP00000382051; ENSG00000138802; Homo sapiens. [Genome view]

GeneID

10427.

KEGG

hsa:10427.

Organism-specific databases

CTD

10427.

GeneCards

GC04P110574.

H-InvDB

HIX0031396.

HGNC

HGNC:10704. SEC24B.

MIM

607184. gene.

PharmGKB

PA35627.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O95487.

HOVERGEN

O95487.

OMA

QGFPSTC

OrthoDB

EOG9ZCWQ1

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpress

O95487.

Bgee

O95487.

CleanEx

HS_SEC24B.

Genevestigator

O95487.

Family and domain databases

InterPro

IPR007123. Gelsoln.
IPR012990. Sec23_24_beta_S.
IPR006900. Sec23_helical.
IPR006896. Sec23_trunk.
IPR006895. Znf_Sec23_Sec24.
[Graphical view]

Pfam

PF00626. Gelsolin. 1 hit.
PF08033. Sec23_BS. 1 hit.
PF04815. Sec23_helical. 1 hit.
PF04811. Sec23_trunk. 1 hit.
PF04810. zf-Sec23_Sec24. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

SC24B_HUMAN

Accession

Primary (citable) accession number: O95487
Secondary accession number(s): B7ZKN4, Q0VG08

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	December 16, 2008
Last modified:	November 24, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.