Reviewed, UniProtKB/Swiss-Prot O95471 (CLD7_HUMAN)

Names and origin

Protein names

Recommended name:
    Claudin-7
      Short name=CLDN-7

Gene names

Name:	CLDN7
Synonyms:	CEPTRL2, CPETRL2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a major role in tight junction-specific obliteration of the intercellular space By similarity.
Subunit structure	Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 By similarity.
Subcellular location	Cell junction › tight junction. Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Induction	By androgens.
Sequence similarities	Belongs to the claudin family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Tight junction
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Gene Ontology (GO)
Biological process	calcium-independent cell-cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Traceable author statement. Source: ProtInc tight junction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	identical protein binding Inferred from sequence or structural similarity. Source: UniProtKB structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95471-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95471-2)

Also known as: t-CLDN-7;

The sequence of this isoform differs from the canonical sequence as follows:
     159-211: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Claudin-7		PRO_0000144750
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Potential
	Topological domain	29 – 81	53	Extracellular Potential
	Transmembrane	82 – 102	21	Potential
	Topological domain	103 – 117	15	Cytoplasmic Potential
	Transmembrane	118 – 138	21	Potential
	Topological domain	139 – 160	22	Extracellular Potential
	Transmembrane	161 – 181	21	Potential
	Topological domain	182 – 211	30	Cytoplasmic Potential
	Region	210 – 211	2	Interactions with TJP1, TJP2 and TJP3 By similarity
Natural variations
	Alternative sequence	159 – 211	53	Missing in isoform 2.		VSP_013230
	Natural variant	133	1	A → T: dbSNP rs17849410.		VAR_030736
	Natural variant	197	1	A → V: dbSNP rs4562.		VAR_014538
Experimental info
	Sequence conflict	18 – 23	6	WVGLVA → GCVSWL in AAP97219. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 29, 2005. Version 3. Checksum: 7F3CC1B963D912E1	FASTA	211	22,390
	10 20 30 40 50 60 MANSGLQLLG FSMALLGWVG LVACTAIPQW QMSSYAGDNI ITAQAMYKGL WMDCVTQSTG 70 80 90 100 110 120 MMSCKMYDSV LALSAALQAT RALMVVSLVL GFLAMFVATM GMKCTRCGGD DKVKKARIAM 130 140 150 160 170 180 GGGIIFIVAG LAALVACSWY GHQIVTDFYN PLIPTNIKYE FGPAIFIGWA GSALVILGGA 190 200 210 LLSCSCPGNE SKAGYRAPRS YPKSNSSKEY V
	Isoform 2 (t-CLDN-7) [UniParc]. Checksum: 19E2D108C134F26E		158	16,837
	10 20 30 40 50 60 MANSGLQLLG FSMALLGWVG LVACTAIPQW QMSSYAGDNI ITAQAMYKGL WMDCVTQSTG 70 80 90 100 110 120 MMSCKMYDSV LALSAALQAT RALMVVSLVL GFLAMFVATM GMKCTRCGGD DKVKKARIAM 130 140 150 GGGIIFIVAG LAALVACSWY GHQIVTDFYN PLIPTNIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Keen T.J. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Colon adenocarcinoma.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-133.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS THR-133 AND VAL-197. Tissue: Mammary gland and Placenta.
[4]	"Cloning and expression of a novel human cDNA homology to murine claudin-1 mRNA." Yue P., Yu L., Bi A.D., Zhang M., He H., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-211 (ISOFORM 1).
[5]	"Regulation of the expression of the prostate-specific antigen by claudin-7." Zheng J.-Y., Yu D., Foroohar M., Ko E., Chan J., Kim N., Chiu R., Pang S. J. Membr. Biol. 194:187-197(2003) [PubMed: 14502431] [Abstract] Cited for: ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
[6]	"Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast." Kominsky S.L., Argani P., Korz D., Evron E., Raman V., Garrett E., Rein A., Sauter G., Kallioniemi O.-P., Sukumar S. Oncogene 22:2021-2033(2003) [PubMed: 12673207] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ011497 mRNA. Translation: CAA09626.1. BT006829 mRNA. Translation: AAP35475.1. BC001055 mRNA. Translation: AAH01055.1. BC071844 mRNA. Translation: AAH71844.1. AF093823 mRNA. Translation: AAP97219.1.
RefSeq	NP_001298.3.
UniGene	Hs.513915
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O95471.
PTM databases
PhosphoSite	O95471.
Genome annotation databases
Ensembl	ENSG00000181885. Homo sapiens. [Contig view]
GeneID	1366.
KEGG	hsa:1366.
Organism-specific databases
H-InvDB	HIX0013110.
HGNC	HGNC:2049. CLDN7.
HPA	CAB013063. HPA014703.
MIM	609131. gene.
PharmGKB	PA26575.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	O95471.
HOVERGEN	O95471.
Gene expression databases
CleanEx	HS_CLDN7.
GermOnline	ENSG00000181885. Homo sapiens.
Family and domain databases
InterPro	IPR006187. Claudin. IPR003552. Claudin7. IPR004031. PMP22/EMP/MP20/Claudin. [Graphical view]
PANTHER	PTHR12002. Claudin. 1 hit. PTHR12002:SF30. Claudin7. 1 hit.
Pfam	PF00822. PMP22_Claudin. 1 hit. [Graphical view]
PRINTS	PR01077. CLAUDIN. PR01381. CLAUDIN7.
PROSITE	PS01346. CLAUDIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	O95471.
NextBio	5535.
SOURCE	Search...

Entry information

Entry name

CLD7_HUMAN

Accession

Primary (citable) accession number: O95471
Secondary accession number(s): Q6IPN3, Q7Z4Y7, Q9BVN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 29, 2005
Last modified:	November 25, 2008
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)