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Protein

Sodium-dependent phosphate transport protein 2B

Gene

SLC34A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actively transporting phosphate into cells via Na+ cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.

pH dependencei

Optimum pH is 6.6.1 Publication

GO - Molecular functioni

  • inorganic phosphate transmembrane transporter activity Source: GO_Central
  • phosphate ion binding Source: UniProtKB
  • sodium:phosphate symporter activity Source: UniProtKB
  • sodium-dependent phosphate transmembrane transporter activity Source: UniProtKB
  • sodium ion binding Source: UniProtKB

GO - Biological processi

  • cellular phosphate ion homeostasis Source: UniProtKB
  • cellular protein metabolic process Source: Reactome
  • in utero embryonic development Source: Ensembl
  • phosphate ion transport Source: UniProtKB
  • response to estrogen Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciZFISH:ENSG00000157765-MONOMER.
ReactomeiR-HSA-427589. Type II Na+/Pi cotransporters.
R-HSA-5683826. Surfactant metabolism.
SIGNORiO95436.

Protein family/group databases

TCDBi2.A.58.1.4. the phosphate:na(+) symporter (pnas) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transport protein 2B
Short name:
Sodium-phosphate transport protein 2B
Alternative name(s):
Na(+)-dependent phosphate cotransporter 2B
NaPi3b
Sodium/phosphate cotransporter 2B
Short name:
Na(+)/Pi cotransporter 2B
Short name:
NaPi-2b
Solute carrier family 34 member 2
Gene namesi
Name:SLC34A2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:11020. SLC34A2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 100CytoplasmicSequence analysisAdd BLAST100
Transmembranei101 – 121Helical; Name=M1Sequence analysisAdd BLAST21
Topological domaini122 – 135ExtracellularSequence analysisAdd BLAST14
Transmembranei136 – 156Helical; Name=M2Sequence analysisAdd BLAST21
Topological domaini157 – 212CytoplasmicSequence analysisAdd BLAST56
Transmembranei213 – 233Helical; Name=M3Sequence analysisAdd BLAST21
Topological domaini234 – 362ExtracellularSequence analysisAdd BLAST129
Transmembranei363 – 383Helical; Name=M4Sequence analysisAdd BLAST21
Topological domaini384 – 407CytoplasmicSequence analysisAdd BLAST24
Transmembranei408 – 428Helical; Name=M5Sequence analysisAdd BLAST21
Topological domaini429 – 485ExtracellularSequence analysisAdd BLAST57
Transmembranei486 – 506Helical; Name=M6Sequence analysisAdd BLAST21
Topological domaini507 – 525CytoplasmicSequence analysisAdd BLAST19
Transmembranei526 – 546Helical; Name=M7Sequence analysisAdd BLAST21
Topological domaini547 – 552ExtracellularSequence analysis6
Transmembranei553 – 573Helical; Name=M8Sequence analysisAdd BLAST21
Topological domaini574 – 689CytoplasmicSequence analysisAdd BLAST116

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • brush border Source: GO_Central
  • brush border membrane Source: UniProtKB
  • cytoplasm Source: GO_Central
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: Reactome
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Pulmonary alveolar microlithiasis (PALM)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionRare disease characterized by the deposition of calcium phosphate microliths throughout the lungs. Most patients are asymptomatic for several years or even for decades and generally, the diagnosis is incidental to clinical investigations unrelated to the disease. Cases with early-onset or rapid progression are rare. A 'sandstorm-appearing' chest roentgenogram is a typical diagnostic finding. The onset of this potentially lethal disease varies from the neonatal period to old age and the disease follows a long-term, progressive course, resulting in a slow deterioration of lung functions. Pulmonary alveolar microlithiasis is a recessive monogenic disease with full penetrance.
See also OMIM:265100
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030677106G → R in PALM. 1 PublicationCorresponds to variant rs137853142dbSNPEnsembl.1

A chromosomal aberration involving SLC34A2 is found in a glioblastoma multiforme cell line U-118MG. Results in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126 – 127Breakpoint for translocation to form a SLC34A2-ROS1 fusion protein2

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi10568.
MalaCardsiSLC34A2.
MIMi265100. phenotype.
OpenTargetsiENSG00000157765.
Orphaneti60025. Pulmonary alveolar microlithiasis.
PharmGKBiPA35888.

Polymorphism and mutation databases

BioMutaiSLC34A2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686131 – 690Sodium-dependent phosphate transport protein 2BAdd BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi303 ↔ 350By similarity
Glycosylationi308N-linked (GlcNAc...)Sequence analysis1
Glycosylationi313N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi340N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO95436.
PaxDbiO95436.
PeptideAtlasiO95436.
PRIDEiO95436.

PTM databases

iPTMnetiO95436.
PhosphoSitePlusiO95436.

Expressioni

Tissue specificityi

Highly expressed in lung. Also detected in pancreas, kidney, small intestine, ovary, testis, prostate and mammary gland. In lung, it is found in alveolar type II cells but not in bronchiolar epithelium.2 Publications

Inductioni

Down-regulated by EGF.1 Publication

Gene expression databases

BgeeiENSG00000157765.
CleanExiHS_SLC34A2.
ExpressionAtlasiO95436. baseline and differential.
GenevisibleiO95436. HS.

Organism-specific databases

HPAiHPA037989.

Interactioni

Protein-protein interaction databases

BioGridi115819. 14 interactors.
IntActiO95436. 1 interactor.
STRINGi9606.ENSP00000371483.

Structurei

3D structure databases

ProteinModelPortaliO95436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi464 – 467Poly-Thr4
Compositional biasi612 – 644Cys-richAdd BLAST33

Sequence similaritiesi

Belongs to the SLC34A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
GeneTreeiENSGT00390000005032.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiO95436.
KOiK14683.
OMAiKTECTAL.
OrthoDBiEOG091G0DR5.
PhylomeDBiO95436.
TreeFamiTF313981.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029852. Na/Pi_transpt_2B.
[Graphical view]
PANTHERiPTHR10010:SF23. PTHR10010:SF23. 2 hits.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95436-1) [UniParc]FASTAAdd to basket
Also known as: NaPi-3b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPWPELGDA QPNPDKYLEG AAGQQPTAPD KSKETNKTDN TEAPVTKIEL
60 70 80 90 100
LPSYSTATLI DEPTEVDDPW NLPTLQDSGI KWSERDTKGK ILCFFQGIGR
110 120 130 140 150
LILLLGFLYF FVCSLDILSS AFQLVGGKMA GQFFSNSSIM SNPLLGLVIG
160 170 180 190 200
VLVTVLVQSS STSTSIVVSM VSSSLLTVRA AIPIIMGANI GTSITNTIVA
210 220 230 240 250
LMQVGDRSEF RRAFAGATVH DFFNWLSVLV LLPVEVATHY LEIITQLIVE
260 270 280 290 300
SFHFKNGEDA PDLLKVITKP FTKLIVQLDK KVISQIAMND EKAKNKSLVK
310 320 330 340 350
IWCKTFTNKT QINVTVPSTA NCTSPSLCWT DGIQNWTMKN VTYKENIAKC
360 370 380 390 400
QHIFVNFHLP DLAVGTILLI LSLLVLCGCL IMIVKILGSV LKGQVATVIK
410 420 430 440 450
KTINTDFPFP FAWLTGYLAI LVGAGMTFIV QSSSVFTSAL TPLIGIGVIT
460 470 480 490 500
IERAYPLTLG SNIGTTTTAI LAALASPGNA LRSSLQIALC HFFFNISGIL
510 520 530 540 550
LWYPIPFTRL PIRMAKGLGN ISAKYRWFAV FYLIIFFFLI PLTVFGLSLA
560 570 580 590 600
GWRVLVGVGV PVVFIIILVL CLRLLQSRCP RVLPKKLQNW NFLPLWMRSL
610 620 630 640 650
KPWDAVVSKF TGCFQMRCCC CCRVCCRACC LLCDCPKCCR CSKCCEDLEE
660 670 680 690
AQEGQDVPVK APETFDNITI SREAQGEVPA SDSKTECTAL
Length:690
Mass (Da):75,759
Last modified:November 30, 2010 - v3
Checksum:i3BDB1920CA92C035
GO
Isoform 2 (identifier: O95436-2) [UniParc]FASTAAdd to basket
Also known as: NaPi-2b, NaPi-IIb

The sequence of this isoform differs from the canonical sequence as follows:
     38-39: TD → N

Show »
Length:689
Mass (Da):75,657
Checksum:iB8D40075B1266490
GO

Sequence cautioni

The sequence BAC11354 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228V → L in AAF31328 (PubMed:10610722).Curated1
Sequence conflicti228V → L in AAL55657 (PubMed:11171583).Curated1
Sequence conflicti330T → V in BAC11354 (PubMed:14702039).Curated1
Sequence conflicti590 – 595Missing in BAC11354 (PubMed:14702039).Curated6
Sequence conflicti620C → Y in AAC98695 (PubMed:10329428).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03415645V → A.Corresponds to variant rs35426730dbSNPEnsembl.1
Natural variantiVAR_030677106G → R in PALM. 1 PublicationCorresponds to variant rs137853142dbSNPEnsembl.1
Natural variantiVAR_030678634D → G.4 PublicationsCorresponds to variant rs6448389dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01675538 – 39TD → N in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111856 mRNA. Translation: AAC98695.1.
AF146796 mRNA. Translation: AAF31328.1.
AC092436 Genomic DNA. No translation available.
BC142704 mRNA. Translation: AAI42705.1.
BC146666 mRNA. Translation: AAI46667.1.
AH011306 Genomic DNA. Translation: AAL55657.1.
AK075015 mRNA. Translation: BAC11354.1. Different initiation.
CCDSiCCDS3435.1. [O95436-1]
CCDS54750.1. [O95436-2]
RefSeqiNP_001171469.1. NM_001177998.1.
NP_001171470.1. NM_001177999.1.
NP_006415.2. NM_006424.2.
UniGeneiHs.479372.

Genome annotation databases

EnsembliENST00000382051; ENSP00000371483; ENSG00000157765. [O95436-1]
ENST00000503434; ENSP00000423021; ENSG00000157765. [O95436-2]
ENST00000504570; ENSP00000425501; ENSG00000157765. [O95436-2]
GeneIDi10568.
KEGGihsa:10568.
UCSCiuc003grr.4. human. [O95436-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111856 mRNA. Translation: AAC98695.1.
AF146796 mRNA. Translation: AAF31328.1.
AC092436 Genomic DNA. No translation available.
BC142704 mRNA. Translation: AAI42705.1.
BC146666 mRNA. Translation: AAI46667.1.
AH011306 Genomic DNA. Translation: AAL55657.1.
AK075015 mRNA. Translation: BAC11354.1. Different initiation.
CCDSiCCDS3435.1. [O95436-1]
CCDS54750.1. [O95436-2]
RefSeqiNP_001171469.1. NM_001177998.1.
NP_001171470.1. NM_001177999.1.
NP_006415.2. NM_006424.2.
UniGeneiHs.479372.

3D structure databases

ProteinModelPortaliO95436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115819. 14 interactors.
IntActiO95436. 1 interactor.
STRINGi9606.ENSP00000371483.

Protein family/group databases

TCDBi2.A.58.1.4. the phosphate:na(+) symporter (pnas) family.

PTM databases

iPTMnetiO95436.
PhosphoSitePlusiO95436.

Polymorphism and mutation databases

BioMutaiSLC34A2.

Proteomic databases

MaxQBiO95436.
PaxDbiO95436.
PeptideAtlasiO95436.
PRIDEiO95436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000382051; ENSP00000371483; ENSG00000157765. [O95436-1]
ENST00000503434; ENSP00000423021; ENSG00000157765. [O95436-2]
ENST00000504570; ENSP00000425501; ENSG00000157765. [O95436-2]
GeneIDi10568.
KEGGihsa:10568.
UCSCiuc003grr.4. human. [O95436-1]

Organism-specific databases

CTDi10568.
DisGeNETi10568.
GeneCardsiSLC34A2.
HGNCiHGNC:11020. SLC34A2.
HPAiHPA037989.
MalaCardsiSLC34A2.
MIMi265100. phenotype.
604217. gene.
neXtProtiNX_O95436.
OpenTargetsiENSG00000157765.
Orphaneti60025. Pulmonary alveolar microlithiasis.
PharmGKBiPA35888.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IE8P. Eukaryota.
COG1283. LUCA.
GeneTreeiENSGT00390000005032.
HOGENOMiHOG000006550.
HOVERGENiHBG006527.
InParanoidiO95436.
KOiK14683.
OMAiKTECTAL.
OrthoDBiEOG091G0DR5.
PhylomeDBiO95436.
TreeFamiTF313981.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000157765-MONOMER.
ReactomeiR-HSA-427589. Type II Na+/Pi cotransporters.
R-HSA-5683826. Surfactant metabolism.
SIGNORiO95436.

Miscellaneous databases

ChiTaRSiSLC34A2. human.
GeneWikiiSLC34A2.
GenomeRNAii10568.
PROiO95436.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000157765.
CleanExiHS_SLC34A2.
ExpressionAtlasiO95436. baseline and differential.
GenevisibleiO95436. HS.

Family and domain databases

InterProiIPR003841. Na/Pi_transpt.
IPR029852. Na/Pi_transpt_2B.
[Graphical view]
PANTHERiPTHR10010:SF23. PTHR10010:SF23. 2 hits.
PfamiPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01013. 2a58. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNPT2B_HUMAN
AccessioniPrimary (citable) accession number: O95436
Secondary accession number(s): A5PL17
, Q8N2K2, Q8WYA9, Q9P0V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.