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Protein

Activator of 90 kDa heat shock protein ATPase homolog 1

Gene

AHSA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360).1 Publication

GO - Molecular functioni

  • ATPase activator activity Source: UniProtKB
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • chaperone binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Activator of 90 kDa heat shock protein ATPase homolog 1
Short name:
AHA1
Alternative name(s):
p38
Gene namesi
Name:AHSA1
Synonyms:C14orf3
ORF Names:HSPC322
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:1189. AHSA1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi10598.
OpenTargetsiENSG00000100591.
PharmGKBiPA25515.

Chemistry databases

ChEMBLiCHEMBL3309113.

Polymorphism and mutation databases

BioMutaiAHSA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002158201 – 338Activator of 90 kDa heat shock protein ATPase homolog 1Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N6-acetyllysineCombined sources1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Modified residuei193PhosphoserineCombined sources1
Modified residuei212N6-acetyllysineCombined sources1
Modified residuei258PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95433.
MaxQBiO95433.
PaxDbiO95433.
PeptideAtlasiO95433.
PRIDEiO95433.

2D gel databases

REPRODUCTION-2DPAGEIPI00030706.

PTM databases

iPTMnetiO95433.
PhosphoSitePlusiO95433.
SwissPalmiO95433.

Expressioni

Tissue specificityi

Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.1 Publication

Inductioni

By heat shock and treatment with the HSP90 inhibitor 17-demethoxygeldanamycin (17AAG).1 Publication

Gene expression databases

BgeeiENSG00000100591.
CleanExiHS_AHSA1.
ExpressionAtlasiO95433. baseline and differential.
GenevisibleiO95433. HS.

Organism-specific databases

HPAiCAB006244.
HPA000903.

Interactioni

Subunit structurei

Interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatitis virus glycoprotein (VSV G). Interacts with GCH1. Interacts with SRPK1. Interacts with FLCN (PubMed:27353360). Interacts with HSP90AA1; the interaction activates HSP90AA1 ATPase activity (PubMed:27353360).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GCH1P307933EBI-448610,EBI-958183
HSP90AA1P079004EBI-448610,EBI-296047
HSP90AB1P082383EBI-448610,EBI-352572

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • chaperone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115846. 157 interactors.
IntActiO95433. 28 interactors.
MINTiMINT-5002315.
STRINGi9606.ENSP00000216479.

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi206 – 217Combined sources12
Helixi219 – 225Combined sources7
Helixi229 – 235Combined sources7
Turni253 – 256Combined sources4
Beta strandi261 – 265Combined sources5
Turni266 – 268Combined sources3
Beta strandi269 – 276Combined sources8
Beta strandi285 – 290Combined sources6
Beta strandi298 – 308Combined sources11
Helixi309 – 317Combined sources9
Turni318 – 323Combined sources6
Helixi324 – 330Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X53NMR-A204-335[»]
ProteinModelPortaliO95433.
SMRiO95433.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95433.

Family & Domainsi

Sequence similaritiesi

Belongs to the AHA1 family.Curated

Phylogenomic databases

eggNOGiKOG2936. Eukaryota.
COG5580. LUCA.
GeneTreeiENSGT00390000006429.
HOGENOMiHOG000242852.
HOVERGENiHBG065806.
InParanoidiO95433.
OMAiVMKWRYN.
OrthoDBiEOG091G0BRP.
PhylomeDBiO95433.
TreeFamiTF313680.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR013538. Activator_of_Hsp90_ATPase.
IPR015310. AHSA1_N.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF09229. Aha1_N. 1 hit.
PF08327. AHSA1. 1 hit.
[Graphical view]
SMARTiSM01000. Aha1_N. 1 hit.
[Graphical view]
SUPFAMiSSF103111. SSF103111. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95433-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKWGEGDPR WIVEERADAT NVNNWHWTER DASNWSTDKL KTLFLAVQVQ
60 70 80 90 100
NEEGKCEVTE VSKLDGEASI NNRKGKLIFF YEWSVKLNWT GTSKSGVQYK
110 120 130 140 150
GHVEIPNLSD ENSVDEVEIS VSLAKDEPDT NLVALMKEEG VKLLREAMGI
160 170 180 190 200
YISTLKTEFT QGMILPTMNG ESVDPVGQPA LKTEERKAKP APSKTQARPV
210 220 230 240 250
GVKIPTCKIT LKETFLTSPE ELYRVFTTQE LVQAFTHAPA TLEADRGGKF
260 270 280 290 300
HMVDGNVSGE FTDLVPEKHI VMKWRFKSWP EGHFATITLT FIDKNGETEL
310 320 330
CMEGRGIPAP EEERTRQGWQ RYYFEGIKQT FGYGARLF
Length:338
Mass (Da):38,274
Last modified:May 1, 1999 - v1
Checksum:iE6B686DDD8D7D729
GO
Isoform 2 (identifier: O95433-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     283-338: HFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF → SHCHSG

Note: No experimental confirmation available.
Show »
Length:288
Mass (Da):32,340
Checksum:i4ACB777F0ED29F4E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67 – 68EA → CL in AAF29000 (PubMed:11042152).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055797283 – 338HFATI…GARLF → SHCHSG in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243310 mRNA. Translation: CAB45684.1.
AF164791 mRNA. Translation: AAF80755.1.
AK300766 mRNA. Translation: BAG62431.1.
AK313824 mRNA. Translation: BAG36559.1.
AF111168 Genomic DNA. Translation: AAD09623.1.
CH471061 Genomic DNA. Translation: EAW81291.1.
BC000321 mRNA. Translation: AAH00321.1.
BC007398 mRNA. Translation: AAH07398.2.
AF161440 mRNA. Translation: AAF29000.1.
CCDSiCCDS9863.1. [O95433-1]
PIRiJC7769.
RefSeqiNP_001308370.1. NM_001321441.1.
NP_036243.1. NM_012111.2. [O95433-1]
UniGeneiHs.204041.

Genome annotation databases

EnsembliENST00000216479; ENSP00000216479; ENSG00000100591. [O95433-1]
ENST00000535854; ENSP00000440108; ENSG00000100591. [O95433-2]
GeneIDi10598.
KEGGihsa:10598.
UCSCiuc001xtw.4. human. [O95433-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243310 mRNA. Translation: CAB45684.1.
AF164791 mRNA. Translation: AAF80755.1.
AK300766 mRNA. Translation: BAG62431.1.
AK313824 mRNA. Translation: BAG36559.1.
AF111168 Genomic DNA. Translation: AAD09623.1.
CH471061 Genomic DNA. Translation: EAW81291.1.
BC000321 mRNA. Translation: AAH00321.1.
BC007398 mRNA. Translation: AAH07398.2.
AF161440 mRNA. Translation: AAF29000.1.
CCDSiCCDS9863.1. [O95433-1]
PIRiJC7769.
RefSeqiNP_001308370.1. NM_001321441.1.
NP_036243.1. NM_012111.2. [O95433-1]
UniGeneiHs.204041.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X53NMR-A204-335[»]
ProteinModelPortaliO95433.
SMRiO95433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115846. 157 interactors.
IntActiO95433. 28 interactors.
MINTiMINT-5002315.
STRINGi9606.ENSP00000216479.

Chemistry databases

ChEMBLiCHEMBL3309113.

PTM databases

iPTMnetiO95433.
PhosphoSitePlusiO95433.
SwissPalmiO95433.

Polymorphism and mutation databases

BioMutaiAHSA1.

2D gel databases

REPRODUCTION-2DPAGEIPI00030706.

Proteomic databases

EPDiO95433.
MaxQBiO95433.
PaxDbiO95433.
PeptideAtlasiO95433.
PRIDEiO95433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000216479; ENSP00000216479; ENSG00000100591. [O95433-1]
ENST00000535854; ENSP00000440108; ENSG00000100591. [O95433-2]
GeneIDi10598.
KEGGihsa:10598.
UCSCiuc001xtw.4. human. [O95433-1]

Organism-specific databases

CTDi10598.
DisGeNETi10598.
GeneCardsiAHSA1.
HGNCiHGNC:1189. AHSA1.
HPAiCAB006244.
HPA000903.
MIMi608466. gene.
neXtProtiNX_O95433.
OpenTargetsiENSG00000100591.
PharmGKBiPA25515.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2936. Eukaryota.
COG5580. LUCA.
GeneTreeiENSGT00390000006429.
HOGENOMiHOG000242852.
HOVERGENiHBG065806.
InParanoidiO95433.
OMAiVMKWRYN.
OrthoDBiEOG091G0BRP.
PhylomeDBiO95433.
TreeFamiTF313680.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000100591-MONOMER.

Miscellaneous databases

ChiTaRSiAHSA1. human.
EvolutionaryTraceiO95433.
GeneWikiiAHSA1.
GenomeRNAii10598.
PROiO95433.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100591.
CleanExiHS_AHSA1.
ExpressionAtlasiO95433. baseline and differential.
GenevisibleiO95433. HS.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR013538. Activator_of_Hsp90_ATPase.
IPR015310. AHSA1_N.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF09229. Aha1_N. 1 hit.
PF08327. AHSA1. 1 hit.
[Graphical view]
SMARTiSM01000. Aha1_N. 1 hit.
[Graphical view]
SUPFAMiSSF103111. SSF103111. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAHSA1_HUMAN
AccessioniPrimary (citable) accession number: O95433
Secondary accession number(s): B2R9L2
, B4DUR9, Q96IL6, Q9P060
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.