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O95429 (BAG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
BAG family molecular chaperone regulator 4

Short name=BAG-4
Alternative name(s):
Bcl-2-associated athanogene 4
Silencer of death domains
Gene names
Name:BAG4
Synonyms:SODD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release By similarity. Prevents constitutive TNFRSF1A signaling.

Subunit structure

Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to the death domain of TNFRSF1A in the absence of TNF and thereby prevents binding of adapter molecules such as TRADD or TRAF2. Binds to the death domain of TNFRSF12. Ref.2 Ref.7

Subcellular location

Cytoplasm.

Tissue specificity

Ubiquitous.

Sequence similarities

Contains 1 BAG domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Molecular functionChaperone
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to epidermal growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from direct assay PubMed 21712384. Source: UniProtKB

intracellular signal transduction

Traceable author statement Ref.2. Source: GOC

negative regulation of apoptotic process

Traceable author statement Ref.2. Source: ProtInc

negative regulation of mRNA modification

Inferred from electronic annotation. Source: Ensembl

negative regulation of phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of actin filament polymerization

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of fibroblast migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

protein folding

Traceable author statement Ref.1. Source: ProtInc

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

protein localization to plasma membrane

Inferred from electronic annotation. Source: Ensembl

ruffle assembly

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Inferred from direct assay PubMed 21712384. Source: UniProtKB

nucleus

Inferred from direct assay PubMed 21712384. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 21712384. Source: UniProtKB

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22681889. Source: UniProtKB

receptor signaling protein activity

Traceable author statement Ref.2. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95429-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95429-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-125: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457BAG family molecular chaperone regulator 4
PRO_0000088870

Regions

Domain379 – 45678BAG

Natural variations

Alternative sequence90 – 12536Missing in isoform 2.
VSP_042741

Experimental info

Mutagenesis4141E → A: Reduces interaction with HSP70. Ref.7
Mutagenesis4241D → A: Abolishes interaction with HSP70. Ref.7
Mutagenesis438 – 4392RK → AA: Reduces interaction with HSP70.
Mutagenesis4461Q → A: Abolishes interaction with HSP70. Ref.7

Secondary structure

....... 457
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: B89D59E8118684A3

FASTA45749,594
        10         20         30         40         50         60 
MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HPPPPLYPLR PEPPQPPISW RVRGGGPAET 

        70         80         90        100        110        120 
TWLGEGGGGD GYYPSGGAWP EPGRAGGSHQ EQPPYPSYNS NYWNSTARSR APYPSTYPVR 

       130        140        150        160        170        180 
PELQGQSLNS YTNGAYGPTY PPGPGANTAS YSGAYYAPGY TQTSYSTEVP STYRSSGNSP 

       190        200        210        220        230        240 
TPVSRWIYPQ QDCQTEAPPL RGQVPGYPPS QNPGMTLPHY PYGDGNRSVP QSGPTVRPQE 

       250        260        270        280        290        300 
DAWASPGAYG MGGRYPWPSS APSAPPGNLY MTESTSPWPS SGSPQSPPSP PVQQPKDSSY 

       310        320        330        340        350        360 
PYSQSDQSMN RHNFPCSVHQ YESSGTVNND DSDLLDSQVQ YSAEPQLYGN ATSDHPNNQD 

       370        380        390        400        410        420 
QSSSLPEECV PSDESTPPSI KKIIHVLEKV QYLEQEVEEF VGKKTDKAYW LLEEMLTKEL 

       430        440        450 
LELDSVETGG QDSVRQARKE AVCKIQAILE KLEKKGL 

« Hide

Isoform 2 [UniParc].

Checksum: 3E39EF28E280E94A
Show »

FASTA42145,410

References

« Hide 'large scale' references
[1]"An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators."
Takayama S., Xie Z., Reed J.C.
J. Biol. Chem. 274:781-786(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Leukemic T-cell.
[2]"Prevention of constitutive TNF receptor 1 signaling by silencer of death domains."
Jiang Y., Woronicz J.D., Liu W., Goeddel D.V.
Science 283:543-546(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH TNFRSF1A AND TNFRSF12.
Tissue: Leukemic T-cell.
[3]Erratum
Jiang Y., Woronicz J.D., Liu W., Goeddel D.V.
Science 283:1852-1852(1999)
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Trachea.
[5]"DNA sequence and analysis of human chromosome 8."
Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. expand/collapse author list , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[7]"BAG4/SODD protein contains a short BAG domain."
Briknarova K., Takayama S., Homma S., Baker K., Cabezas E., Hoyt D.W., Li Z., Satterthwait A.C., Ely K.R.
J. Biol. Chem. 277:31172-31178(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 376-457, INTERACTION WITH HSP70, MUTAGENESIS OF GLU-414; ASP-424; 438-ARG-LYS-439 AND GLN-446.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF095194 mRNA. Translation: AAD16123.2.
AF111116 mRNA. Translation: AAD05226.1.
AK304072 mRNA. Translation: BAG64979.1.
AC084024 Genomic DNA. No translation available.
BC038505 mRNA. Translation: AAH38505.2.
RefSeqNP_001191807.1. NM_001204878.1.
NP_004865.1. NM_004874.3.
UniGeneHs.194726.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M62NMR-A376-457[»]
1M7KNMR-A358-456[»]
ProteinModelPortalO95429.
SMRO95429. Positions 376-456.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114906. 19 interactions.
IntActO95429. 3 interactions.
MINTMINT-209028.
STRING9606.ENSP00000287322.

PTM databases

PhosphoSiteO95429.

Proteomic databases

PaxDbO95429.
PRIDEO95429.

Protocols and materials databases

DNASU9530.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000287322; ENSP00000287322; ENSG00000156735. [O95429-1]
ENST00000432471; ENSP00000393298; ENSG00000156735. [O95429-2]
GeneID9530.
KEGGhsa:9530.
UCSCuc003xky.2. human. [O95429-1]
uc003xkz.2. human. [O95429-2]

Organism-specific databases

CTD9530.
GeneCardsGC08P038051.
HGNCHGNC:940. BAG4.
HPACAB013716.
HPA018951.
MIM603884. gene.
neXtProtNX_O95429.
PharmGKBPA25240.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG275247.
HOGENOMHOG000290673.
HOVERGENHBG004809.
InParanoidO95429.
KOK09558.
OMAYTPGYTQ.
OrthoDBEOG75B85S.
PhylomeDBO95429.
TreeFamTF102013.

Gene expression databases

ArrayExpressO95429.
BgeeO95429.
CleanExHS_BAG4.
GenevestigatorO95429.

Family and domain databases

Gene3D1.20.58.120. 1 hit.
InterProIPR003103. BAG_domain.
[Graphical view]
PfamPF02179. BAG. 1 hit.
[Graphical view]
SMARTSM00264. BAG. 1 hit.
[Graphical view]
PROSITEPS51035. BAG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO95429.
GeneWikiBAG4.
GenomeRNAi9530.
NextBio35728.
PROO95429.
SOURCESearch...

Entry information

Entry nameBAG4_HUMAN
AccessionPrimary (citable) accession number: O95429
Secondary accession number(s): B4E217, O95818
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM