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O95429

- BAG4_HUMAN

UniProt

O95429 - BAG4_HUMAN

Protein

BAG family molecular chaperone regulator 4

Gene

BAG4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release By similarity. Prevents constitutive TNFRSF1A signaling. Negative regulator of PARK2 translocation to damaged mitochondria.By similarity1 Publication

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. receptor signaling protein activity Source: ProtInc

    GO - Biological processi

    1. cellular response to epidermal growth factor stimulus Source: Ensembl
    2. cellular response to tumor necrosis factor Source: UniProtKB
    3. intracellular signal transduction Source: GOC
    4. negative regulation of apoptotic process Source: ProtInc
    5. negative regulation of mRNA modification Source: Ensembl
    6. negative regulation of phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity Source: Ensembl
    7. positive regulation of actin filament polymerization Source: Ensembl
    8. positive regulation of cell adhesion Source: Ensembl
    9. positive regulation of fibroblast migration Source: Ensembl
    10. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
    11. positive regulation of protein kinase B signaling Source: Ensembl
    12. positive regulation of stress fiber assembly Source: Ensembl
    13. protein folding Source: ProtInc
    14. protein heterooligomerization Source: Ensembl
    15. protein localization to plasma membrane Source: Ensembl
    16. ruffle assembly Source: Ensembl

    Keywords - Molecular functioni

    Chaperone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    BAG family molecular chaperone regulator 4
    Short name:
    BAG-4
    Alternative name(s):
    Bcl-2-associated athanogene 4
    Silencer of death domains
    Gene namesi
    Name:BAG4
    Synonyms:SODD
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:940. BAG4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi414 – 4141E → A: Reduces interaction with HSP70. 1 Publication
    Mutagenesisi424 – 4241D → A: Abolishes interaction with HSP70. 1 Publication
    Mutagenesisi438 – 4392RK → AA: Reduces interaction with HSP70.
    Mutagenesisi446 – 4461Q → A: Abolishes interaction with HSP70. 1 Publication

    Organism-specific databases

    PharmGKBiPA25240.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457BAG family molecular chaperone regulator 4PRO_0000088870Add
    BLAST

    Proteomic databases

    MaxQBiO95429.
    PaxDbiO95429.
    PRIDEiO95429.

    PTM databases

    PhosphoSiteiO95429.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiO95429.
    BgeeiO95429.
    CleanExiHS_BAG4.
    GenevestigatoriO95429.

    Organism-specific databases

    HPAiCAB013716.
    HPA018951.

    Interactioni

    Subunit structurei

    Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to the death domain of TNFRSF1A in the absence of TNF and thereby prevents binding of adapter molecules such as TRADD or TRAF2. Binds to the death domain of TNFRSF12. Interacts with PARK2.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q53FC72EBI-2949658,EBI-9356749
    DCP1AQ9NPI63EBI-2949658,EBI-374238
    DNAJC7Q996152EBI-2949658,EBI-357552
    HSPA2P546522EBI-2949658,EBI-356991

    Protein-protein interaction databases

    BioGridi114906. 21 interactions.
    IntActiO95429. 26 interactions.
    MINTiMINT-209028.
    STRINGi9606.ENSP00000287322.

    Structurei

    Secondary structure

    1
    457
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi380 – 39920
    Helixi407 – 42317
    Helixi432 – 45625

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M62NMR-A376-457[»]
    1M7KNMR-A358-456[»]
    ProteinModelPortaliO95429.
    SMRiO95429. Positions 376-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO95429.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini379 – 45678BAGPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BAG domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG275247.
    HOGENOMiHOG000290673.
    HOVERGENiHBG004809.
    InParanoidiO95429.
    KOiK09558.
    OMAiYTPGYTQ.
    OrthoDBiEOG75B85S.
    PhylomeDBiO95429.
    TreeFamiTF102013.

    Family and domain databases

    Gene3Di1.20.58.120. 1 hit.
    InterProiIPR003103. BAG_domain.
    [Graphical view]
    PfamiPF02179. BAG. 1 hit.
    [Graphical view]
    SMARTiSM00264. BAG. 1 hit.
    [Graphical view]
    PROSITEiPS51035. BAG. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95429-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HPPPPLYPLR PEPPQPPISW    50
    RVRGGGPAET TWLGEGGGGD GYYPSGGAWP EPGRAGGSHQ EQPPYPSYNS 100
    NYWNSTARSR APYPSTYPVR PELQGQSLNS YTNGAYGPTY PPGPGANTAS 150
    YSGAYYAPGY TQTSYSTEVP STYRSSGNSP TPVSRWIYPQ QDCQTEAPPL 200
    RGQVPGYPPS QNPGMTLPHY PYGDGNRSVP QSGPTVRPQE DAWASPGAYG 250
    MGGRYPWPSS APSAPPGNLY MTESTSPWPS SGSPQSPPSP PVQQPKDSSY 300
    PYSQSDQSMN RHNFPCSVHQ YESSGTVNND DSDLLDSQVQ YSAEPQLYGN 350
    ATSDHPNNQD QSSSLPEECV PSDESTPPSI KKIIHVLEKV QYLEQEVEEF 400
    VGKKTDKAYW LLEEMLTKEL LELDSVETGG QDSVRQARKE AVCKIQAILE 450
    KLEKKGL 457
    Length:457
    Mass (Da):49,594
    Last modified:May 1, 1999 - v1
    Checksum:iB89D59E8118684A3
    GO
    Isoform 2 (identifier: O95429-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         90-125: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:421
    Mass (Da):45,410
    Checksum:i3E39EF28E280E94A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei90 – 12536Missing in isoform 2. 1 PublicationVSP_042741Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095194 mRNA. Translation: AAD16123.2.
    AF111116 mRNA. Translation: AAD05226.1.
    AK304072 mRNA. Translation: BAG64979.1.
    AC084024 Genomic DNA. No translation available.
    BC038505 mRNA. Translation: AAH38505.2.
    CCDSiCCDS56533.1. [O95429-2]
    CCDS6104.1. [O95429-1]
    RefSeqiNP_001191807.1. NM_001204878.1. [O95429-2]
    NP_004865.1. NM_004874.3. [O95429-1]
    UniGeneiHs.194726.

    Genome annotation databases

    EnsembliENST00000287322; ENSP00000287322; ENSG00000156735. [O95429-1]
    ENST00000432471; ENSP00000393298; ENSG00000156735. [O95429-2]
    GeneIDi9530.
    KEGGihsa:9530.
    UCSCiuc003xky.2. human. [O95429-1]
    uc003xkz.2. human. [O95429-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095194 mRNA. Translation: AAD16123.2 .
    AF111116 mRNA. Translation: AAD05226.1 .
    AK304072 mRNA. Translation: BAG64979.1 .
    AC084024 Genomic DNA. No translation available.
    BC038505 mRNA. Translation: AAH38505.2 .
    CCDSi CCDS56533.1. [O95429-2 ]
    CCDS6104.1. [O95429-1 ]
    RefSeqi NP_001191807.1. NM_001204878.1. [O95429-2 ]
    NP_004865.1. NM_004874.3. [O95429-1 ]
    UniGenei Hs.194726.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M62 NMR - A 376-457 [» ]
    1M7K NMR - A 358-456 [» ]
    ProteinModelPortali O95429.
    SMRi O95429. Positions 376-456.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114906. 21 interactions.
    IntActi O95429. 26 interactions.
    MINTi MINT-209028.
    STRINGi 9606.ENSP00000287322.

    PTM databases

    PhosphoSitei O95429.

    Proteomic databases

    MaxQBi O95429.
    PaxDbi O95429.
    PRIDEi O95429.

    Protocols and materials databases

    DNASUi 9530.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000287322 ; ENSP00000287322 ; ENSG00000156735 . [O95429-1 ]
    ENST00000432471 ; ENSP00000393298 ; ENSG00000156735 . [O95429-2 ]
    GeneIDi 9530.
    KEGGi hsa:9530.
    UCSCi uc003xky.2. human. [O95429-1 ]
    uc003xkz.2. human. [O95429-2 ]

    Organism-specific databases

    CTDi 9530.
    GeneCardsi GC08P038051.
    HGNCi HGNC:940. BAG4.
    HPAi CAB013716.
    HPA018951.
    MIMi 603884. gene.
    neXtProti NX_O95429.
    PharmGKBi PA25240.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG275247.
    HOGENOMi HOG000290673.
    HOVERGENi HBG004809.
    InParanoidi O95429.
    KOi K09558.
    OMAi YTPGYTQ.
    OrthoDBi EOG75B85S.
    PhylomeDBi O95429.
    TreeFami TF102013.

    Miscellaneous databases

    EvolutionaryTracei O95429.
    GeneWikii BAG4.
    GenomeRNAii 9530.
    NextBioi 35728.
    PROi O95429.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95429.
    Bgeei O95429.
    CleanExi HS_BAG4.
    Genevestigatori O95429.

    Family and domain databases

    Gene3Di 1.20.58.120. 1 hit.
    InterProi IPR003103. BAG_domain.
    [Graphical view ]
    Pfami PF02179. BAG. 1 hit.
    [Graphical view ]
    SMARTi SM00264. BAG. 1 hit.
    [Graphical view ]
    PROSITEi PS51035. BAG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators."
      Takayama S., Xie Z., Reed J.C.
      J. Biol. Chem. 274:781-786(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Leukemic T-cell.
    2. "Prevention of constitutive TNF receptor 1 signaling by silencer of death domains."
      Jiang Y., Woronicz J.D., Liu W., Goeddel D.V.
      Science 283:543-546(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH TNFRSF1A AND TNFRSF12.
      Tissue: Leukemic T-cell.
    3. Erratum
      Jiang Y., Woronicz J.D., Liu W., Goeddel D.V.
      Science 283:1852-1852(1999)
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Trachea.
    5. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    7. "High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy."
      Hasson S.A., Kane L.A., Yamano K., Huang C.H., Sliter D.A., Buehler E., Wang C., Heman-Ackah S.M., Hessa T., Guha R., Martin S.E., Youle R.J.
      Nature 504:291-295(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PARK2.
    8. Cited for: STRUCTURE BY NMR OF 376-457, INTERACTION WITH HSP70, MUTAGENESIS OF GLU-414; ASP-424; 438-ARG-LYS-439 AND GLN-446.

    Entry informationi

    Entry nameiBAG4_HUMAN
    AccessioniPrimary (citable) accession number: O95429
    Secondary accession number(s): B4E217, O95818
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3