O95429 (BAG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BAG family molecular chaperone regulator 4
Short name=BAG-4

Alternative name(s):
Bcl-2-associated athanogene 4
Silencer of death domains

Gene names

Name:	BAG4
Synonyms:	SODD

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release By similarity. Prevents constitutive TNFRSF1A signaling.
Subunit structure	Binds to the ATPase domain of HSP/HSC70 chaperones. Binds to the death domain of TNFRSF1A in the absence of TNF and thereby prevents binding of adapter molecules such as TRADD or TRAF2. Binds to the death domain of TNFRSF12. Ref.2 Ref.7
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitous.
Sequence similarities	Contains 1 BAG domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to epidermal growth factor stimulus Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from direct assay PubMed 21712384. Source: UniProtKB negative regulation of apoptotic process Traceable author statement Ref.2. Source: ProtInc negative regulation of phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: Compara positive regulation of actin filament polymerization Inferred from electronic annotation. Source: Compara positive regulation of cell adhesion Inferred from electronic annotation. Source: Compara positive regulation of fibroblast migration Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara positive regulation of stress fiber assembly Inferred from electronic annotation. Source: Compara protein folding Traceable author statement Ref.1. Source: ProtInc protein localization to plasma membrane Inferred from electronic annotation. Source: Compara ruffle assembly Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay PubMed 21712384. Source: UniProtKB nucleus Inferred from direct assay PubMed 21712384. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 21712384. Source: UniProtKB
Molecular_function	receptor signaling protein activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O95429-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O95429-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-125: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 457

457

BAG family molecular chaperone regulator 4

PRO_0000088870

Regions

Domain

379 – 456

78

BAG

Natural variations

Alternative sequence

90 – 125

36

Missing in isoform 2.

VSP_042741

Experimental info

Mutagenesis

414

1

E → A: Reduces interaction with HSP70. Ref.7

Mutagenesis

424

1

D → A: Abolishes interaction with HSP70. Ref.7

Mutagenesis

438 – 439

2

RK → AA: Reduces interaction with HSP70.

Mutagenesis

446

1

Q → A: Abolishes interaction with HSP70. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

457

Helix Strand Turn

Details...

Helix

380 – 399

20

Helix

407 – 423

17

Helix

432 – 456

25

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: B89D59E8118684A3	FASTA	457	49,594
	10 20 30 40 50 60 MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HPPPPLYPLR PEPPQPPISW RVRGGGPAET 70 80 90 100 110 120 TWLGEGGGGD GYYPSGGAWP EPGRAGGSHQ EQPPYPSYNS NYWNSTARSR APYPSTYPVR 130 140 150 160 170 180 PELQGQSLNS YTNGAYGPTY PPGPGANTAS YSGAYYAPGY TQTSYSTEVP STYRSSGNSP 190 200 210 220 230 240 TPVSRWIYPQ QDCQTEAPPL RGQVPGYPPS QNPGMTLPHY PYGDGNRSVP QSGPTVRPQE 250 260 270 280 290 300 DAWASPGAYG MGGRYPWPSS APSAPPGNLY MTESTSPWPS SGSPQSPPSP PVQQPKDSSY 310 320 330 340 350 360 PYSQSDQSMN RHNFPCSVHQ YESSGTVNND DSDLLDSQVQ YSAEPQLYGN ATSDHPNNQD 370 380 390 400 410 420 QSSSLPEECV PSDESTPPSI KKIIHVLEKV QYLEQEVEEF VGKKTDKAYW LLEEMLTKEL 430 440 450 LELDSVETGG QDSVRQARKE AVCKIQAILE KLEKKGL
	Isoform 2 [UniParc]. Checksum: 3E39EF28E280E94A	FASTA	421	45,410
	10 20 30 40 50 60 MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HPPPPLYPLR PEPPQPPISW RVRGGGPAET 70 80 90 100 110 120 TWLGEGGGGD GYYPSGGAWP EPGRAGGSHQ SLNSYTNGAY GPTYPPGPGA NTASYSGAYY 130 140 150 160 170 180 APGYTQTSYS TEVPSTYRSS GNSPTPVSRW IYPQQDCQTE APPLRGQVPG YPPSQNPGMT 190 200 210 220 230 240 LPHYPYGDGN RSVPQSGPTV RPQEDAWASP GAYGMGGRYP WPSSAPSAPP GNLYMTESTS 250 260 270 280 290 300 PWPSSGSPQS PPSPPVQQPK DSSYPYSQSD QSMNRHNFPC SVHQYESSGT VNNDDSDLLD 310 320 330 340 350 360 SQVQYSAEPQ LYGNATSDHP NNQDQSSSLP EECVPSDEST PPSIKKIIHV LEKVQYLEQE 370 380 390 400 410 420 VEEFVGKKTD KAYWLLEEML TKELLELDSV ETGGQDSVRQ ARKEAVCKIQ AILEKLEKKG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators." Takayama S., Xie Z., Reed J.C. J. Biol. Chem. 274:781-786(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Leukemic T-cell.
[2]	"Prevention of constitutive TNF receptor 1 signaling by silencer of death domains." Jiang Y., Woronicz J.D., Liu W., Goeddel D.V. Science 283:543-546(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH TNFRSF1A AND TNFRSF12. Tissue: Leukemic T-cell.
[3]	Erratum Jiang Y., Woronicz J.D., Liu W., Goeddel D.V. Science 283:1852-1852(1999)
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Trachea.
[5]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[7]	"BAG4/SODD protein contains a short BAG domain." Briknarova K., Takayama S., Homma S., Baker K., Cabezas E., Hoyt D.W., Li Z., Satterthwait A.C., Ely K.R. J. Biol. Chem. 277:31172-31178(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 376-457, INTERACTION WITH HSP70, MUTAGENESIS OF GLU-414; ASP-424; 438-ARG-LYS-439 AND GLN-446.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF095194 mRNA. Translation: AAD16123.2.
AF111116 mRNA. Translation: AAD05226.1.
AK304072 mRNA. Translation: BAG64979.1.
AC084024 Genomic DNA. No translation available.
BC038505 mRNA. Translation: AAH38505.2.

IPI

IPI00030695.
IPI00798092.

RefSeq

NP_001191807.1. NM_001204878.1.
NP_004865.1. NM_004874.3.

UniGene

Hs.194726.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M62	NMR	-	A	376-457	[»]
1M7K	NMR	-	A	358-456	[»]

ProteinModelPortal

O95429.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95429. 3 interactions.

MINT

MINT-209028.

STRING

9606.ENSP00000287322.

PTM databases

PhosphoSite

O95429.

Proteomic databases

PaxDb

O95429.

PRIDE

O95429.

Protocols and materials databases

DNASU

9530.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000287322; ENSP00000287322; ENSG00000156735.
ENST00000432471; ENSP00000393298; ENSG00000156735.

GeneID

9530.

KEGG

hsa:9530.

UCSC

uc003xky.2. human.
uc003xkz.2. human.

Organism-specific databases

CTD

9530.

GeneCards

GC08P038051.

HGNC

HGNC:940. BAG4.

HPA

CAB013716.
HPA018951.

MIM

603884. gene.

neXtProt

NX_O95429.

PharmGKB

PA25240.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG275247.

HOGENOM

HOG000290673.

HOVERGEN

HBG004809.

InParanoid

O95429.

KO

K09558.

OMA

CQTEAAP.

OrthoDB

EOG4PG627.

PhylomeDB

O95429.

Enzyme and pathway databases

Pathway_Interaction_DB

ceramidepathway. Ceramide signaling pathway.
hivnefpathway. HIV-1 Nef: Negative effector of Fas and TNF-alpha.
tnfpathway. TNF receptor signaling pathway.

Gene expression databases

ArrayExpress

O95429.

Bgee

O95429.

CleanEx

HS_BAG4.

Genevestigator

O95429.

GermOnline

ENSG00000156735. Homo sapiens.

Family and domain databases

InterPro

IPR003103. BAG_domain.
[Graphical view]

Pfam

PF02179. BAG. 1 hit.
[Graphical view]

SMART

SM00264. BAG. 1 hit.
[Graphical view]

PROSITE

PS51035. BAG. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O95429.

GenomeRNAi

9530.

NextBio

35728.

SOURCE

Search...

Entry information

Entry name

BAG4_HUMAN

Accession

Primary (citable) accession number: O95429
Secondary accession number(s): B4E217, O95818

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	May 29, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.