O95407 (TNF6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor receptor superfamily member 6B

Alternative name(s):
Decoy receptor 3
Short name=DcR3
Decoy receptor for Fas ligand
M68

Gene names

Name:	TNFRSF6B
Synonyms:	DCR3, TR6
ORF Names:	UNQ186/PRO212

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. Ref.7
Subcellular location	Secreted.
Tissue specificity	Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
Sequence similarities	Contains 4 TNFR-Cys repeats.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular space Non-traceable author statement Ref.2. Source: UniProtKB
Molecular_function	receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TNFSF14	O43557	2	EBI-524171,EBI-524131

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

29

Ref.2

Chain

30 – 300

271

Tumor necrosis factor receptor superfamily member 6B

PRO_0000034570

Regions

Repeat

31 – 70

40

TNFR-Cys 1

Repeat

72 – 113

42

TNFR-Cys 2

Repeat

115 – 150

36

TNFR-Cys 3

Repeat

152 – 193

42

TNFR-Cys 4

Amino acid modifications

Glycosylation

173

1

N-linked (GlcNAc...) Potential

Disulfide bond

49 ↔ 62

Ref.7

Disulfide bond

52 ↔ 70

Ref.7

Disulfide bond

73 ↔ 88

Ref.7

Disulfide bond

91 ↔ 105

Ref.7

Disulfide bond

95 ↔ 113

Ref.7

Disulfide bond

115 ↔ 126

Ref.7

Disulfide bond

132 ↔ 150

Ref.7

Disulfide bond

153 ↔ 168

Ref.7

Disulfide bond

174 ↔ 193

Ref.7

Secondary structure

1

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300

Helix Strand Turn

Details...

Beta strand

35 – 39

5

Beta strand

41 – 43

3

Beta strand

46 – 50

5

Beta strand

56 – 60

5

Beta strand

64 – 66

3

Beta strand

69 – 72

4

Beta strand

81 – 83

3

Beta strand

97 – 103

7

Beta strand

107 – 109

3

Beta strand

112 – 115

4

Beta strand

119 – 122

4

Beta strand

125 – 128

4

Beta strand

136 – 140

5

Beta strand

144 – 146

3

Beta strand

149 – 152

4

Beta strand

163 – 165

3

Turn

175 – 178

4

Beta strand

181 – 184

4

Beta strand

186 – 189

4

Beta strand

191 – 193

3

Sequences

	Sequence		Length	Mass (Da)
	O95407 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: F90AEE33718449AF	FASTA	300	32,680
	10 20 30 40 50 60 MRALEGPGLS LLCLVLALPA LLPVPAVRGV AETPTYPWRD AETGERLVCA QCPPGTFVQR 70 80 90 100 110 120 PCRRDSPTTC GPCPPRHYTQ FWNYLERCRY CNVLCGEREE EARACHATHN RACRCRTGFF 130 140 150 160 170 180 AHAGFCLEHA SCPPGAGVIA PGTPSQNTQC QPCPPGTFSA SSSSSEQCQP HRNCTALGLA 190 200 210 220 230 240 LNVPGSSSHD TLCTSCTGFP LSTRVPGAEE CERAVIDFVA FQDISIKRLQ RLLQALEAPE 250 260 270 280 290 300 GWGPTPRAGR AALQLKLRRR LTELLGAQDG ALLVRLLQAL RVARMPGLER SVRERFLPVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer." Pitti R.M., Marsters S.A., Lawrence D.A., Roy M., Kischkel F.C., Dowd P., Huang A., Donahue C.J., Sherwood S.W., Baldwin D.T., Godowski P.J., Wood W.I., Gurney A.L., Hillan K.J., Cohen R.L., Goddard A.D., Botstein D., Ashkenazi A. Nature 396:699-703(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal lung.
[2]	"A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis." Yu K.-Y., Kwon B., Ni J., Zhai Y., Ebner R., Kwon B.S. J. Biol. Chem. 274:13733-13736(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 30-35. Tissue: Prostate.
[3]	"Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster." Bai C., Connolly B., Metzker M.L., Hilliard C.A., Liu X., Sandig V., Soderman A., Galloway S.M., Liu Q., Austin C.P., Caskey C.T. Proc. Natl. Acad. Sci. U.S.A. 97:1230-1235(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Lung.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Skin.
[7]	"Decoy strategies: the structure of TL1A:DcR3 complex." Zhan C., Patskovsky Y., Yan Q., Li Z., Ramagopal U., Cheng H., Brenowitz M., Hui X., Nathenson S.G., Almo S.C. Structure 19:162-171(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 30-195 IN COMPLEX WITH TNFSF15, FUNCTION, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF104419 mRNA. Translation: AAD03056.1.
AF134240 mRNA. Translation: AAD29688.1.
AF217796 Genomic DNA. Translation: AAF35244.1.
AF217793 mRNA. Translation: AAF33685.1.
AF217794 mRNA. Translation: AAF33686.1.
AY358279 mRNA. Translation: AAQ88646.1.
AL121845 Genomic DNA. Translation: CAC03668.1.
BC017065 mRNA. Translation: AAH17065.1.
BC034349 mRNA. Translation: AAH34349.1.

IPI

IPI00646641.

RefSeq

NP_003814.1. NM_003823.3.

UniGene

Hs.741542.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3K51	X-ray	2.45	B	30-195	[»]
3MHD	X-ray	2.90	D	30-195	[»]
3MI8	X-ray	2.95	D	30-195	[»]
4J6G	X-ray	2.40	C/D	30-195	[»]

ProteinModelPortal

O95407.

ModBase

Search...

Protein-protein interaction databases

IntAct

O95407. 2 interactions.

STRING

9606.ENSP00000359013.

PTM databases

PhosphoSite

O95407.

Proteomic databases

PaxDb

O95407.

PRIDE

O95407.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000369996; ENSP00000359013; ENSG00000243509.

GeneID

8771.

KEGG

hsa:8771.

UCSC

uc002yfy.3. human.

Organism-specific databases

CTD

8771.

GeneCards

GC20P062326.

HGNC

HGNC:11921. TNFRSF6B.

HPA

HPA047154.

MIM

603361. gene.

neXtProt

NX_O95407.

PharmGKB

PA36614.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG295608.

HOGENOM

HOG000154657.

KO

K05143.

OMA

NVICGER.

OrthoDB

EOG4BCDP3.

Gene expression databases

ArrayExpress

O95407.

CleanEx

HS_TNFRSF6B.

Genevestigator

O95407.

GermOnline

ENSG00000026036. Homo sapiens.

Family and domain databases

InterPro

IPR001368. TNFR/NGFR_Cys_rich_reg.
[Graphical view]

Pfam

PF00020. TNFR_c6. 1 hit.
[Graphical view]

SMART

SM00208. TNFR. 4 hits.
[Graphical view]

PROSITE

PS00652. TNFR_NGFR_1. 1 hit.
PS50050. TNFR_NGFR_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O95407.

GenomeRNAi

8771.

NextBio

32884.

SOURCE

Search...

Entry information

Entry name

TNF6B_HUMAN

Accession

Primary (citable) accession number: O95407

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.