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O95402 (MED26_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 26
Alternative name(s):
Activator-recruited cofactor 70 kDa component
Short name=ARC70
Cofactor required for Sp1 transcriptional activation subunit 7
Short name=CRSP complex subunit 7
Mediator complex subunit 26
Transcriptional coactivator CRSP70
Gene names
Name:MED26
Synonyms:ARC70, CRSP7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length600 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Ref.3 Ref.4 Ref.5

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 26 family.

Contains 1 TFIIS N-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EAF1Q96JC95EBI-394392,EBI-769261

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O95402-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O95402-2)

The sequence of this isoform differs from the canonical sequence as follows:
     76-92: LLRSWQKLIEPAHQHEA → LPGWQWACRPRGQPPGA
     93-600: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 600600Mediator of RNA polymerase II transcription subunit 26
PRO_0000079360

Regions

Domain10 – 8778TFIIS N-terminal
Compositional bias228 – 32598Pro-rich

Natural variations

Alternative sequence76 – 9217LLRSW…HQHEA → LPGWQWACRPRGQPPGA in isoform 2.
VSP_028151
Alternative sequence93 – 600508Missing in isoform 2.
VSP_028152

Experimental info

Sequence conflict1561L → F in AAD12722. Ref.1
Sequence conflict2411P → A in AAI27216. Ref.2
Sequence conflict3691R → L in AAI10431. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: 4BEA264A3EB5A407

FASTA60065,446
        10         20         30         40         50         60 
MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE TRLGKLINDV 

        70         80         90        100        110        120 
RKKTKNEELA KRAKKLLRSW QKLIEPAHQH EAALRGLAGA TGSANGGAHN CRPEVGAAGP 

       130        140        150        160        170        180 
PRSIHDLKSR NDLQRLPGQR LDRLGSRKRR GDQRDLGHPG PPPKVSKASH DPLVPNSSPL 

       190        200        210        220        230        240 
PTNGISGSPE SFASSLDGSG HAGPEGSRLE RDENDKHSGK IPVNAVRPHT SSPGLGKPPG 

       250        260        270        280        290        300 
PCLQPKASVL QQLDRVDETP GPPHPKGPPR CSFSPRNSRH EGSFARQQSL YAPKGSVPSP 

       310        320        330        340        350        360 
SPRPQALDAT QVPSPLPLAQ PSTPPVRRLE LLPSAESPVC WLEQPESHQR LAGPGCKAGL 

       370        380        390        400        410        420 
SPAEPLLSRA GFSPDSSKAD SDAASSGGSD SKKKKRYRPR DYTVNLDGQV AEAGVKPVRL 

       430        440        450        460        470        480 
KERKLTFDPM TRQIKPLTQK EPVRADSPVH MEQQSRTELD KQEAKASLQS PFEQTNWKEL 

       490        500        510        520        530        540 
SRNEIIQSYL SRQSSLLSSS GAQTPGAHHF MSEYLKQEES TRQGARQLHV LVPQSPPTDL 

       550        560        570        580        590        600 
PGLTREVTQD DLDRIQASQW PGVNGCQDTQ GNWYDWTQCI SLDPHGDDGR LNILPYVCLD 

« Hide

Isoform 2 [UniParc].

Checksum: 6837BD299DE56B5A
Show »

FASTA9210,359

References

« Hide 'large scale' references
[1]"The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1."
Ryu S., Zhou S., Ladurner A.G., Tjian R.
Nature 397:446-450(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Uterus.
[3]"Composite co-activator ARC mediates chromatin-directed transcriptional activation."
Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN ARC COMPLEX, PROTEIN SEQUENCE OF 294-305 AND 478-489.
[4]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
[5]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED1 AND MED10, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF104253 mRNA. Translation: AAD12722.1.
BC110430 mRNA. Translation: AAI10431.1.
BC127215 mRNA. Translation: AAI27216.1.
RefSeqNP_004822.2. NM_004831.3.
UniGeneHs.715040.

3D structure databases

ProteinModelPortalO95402.
SMRO95402. Positions 9-85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114831. 84 interactions.
DIPDIP-31464N.
IntActO95402. 90 interactions.
STRING9606.ENSP00000263390.

PTM databases

PhosphoSiteO95402.

Proteomic databases

PaxDbO95402.
PRIDEO95402.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263390; ENSP00000263390; ENSG00000105085. [O95402-1]
GeneID9441.
KEGGhsa:9441.
UCSCuc002nen.1. human. [O95402-1]

Organism-specific databases

CTD9441.
GeneCardsGC19M016685.
H-InvDBHIX0202876.
HGNCHGNC:2376. MED26.
HPAHPA044195.
MIM605043. gene.
neXtProtNX_O95402.
PharmGKBPA162395623.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG263228.
HOGENOMHOG000113539.
HOVERGENHBG051124.
InParanoidO95402.
KOK15169.
OMAHFMSEYL.
OrthoDBEOG7X0VGM.
PhylomeDBO95402.
TreeFamTF328436.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressO95402.
BgeeO95402.
CleanExHS_MED26.
GenevestigatorO95402.

Family and domain databases

Gene3D1.20.930.10. 1 hit.
InterProIPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamPF08711. Med26. 1 hit.
[Graphical view]
SMARTSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMSSF47676. SSF47676. 1 hit.
PROSITEPS51319. TFIIS_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMED26. human.
GeneWikiMED26.
GenomeRNAi9441.
NextBio35354.
PMAP-CutDBO95402.
PROO95402.
SOURCESearch...

Entry information

Entry nameMED26_HUMAN
AccessionPrimary (citable) accession number: O95402
Secondary accession number(s): A1A4S3, Q0VGB6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM