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O95402

- MED26_HUMAN

UniProt

O95402 - MED26_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 26

Gene

MED26

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. protein binding Source: IntAct
    3. RNA polymerase II transcription cofactor activity Source: UniProtKB
    4. transcription coactivator activity Source: ProtInc

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    3. transcription initiation from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 26
    Alternative name(s):
    Activator-recruited cofactor 70 kDa component
    Short name:
    ARC70
    Cofactor required for Sp1 transcriptional activation subunit 7
    Short name:
    CRSP complex subunit 7
    Mediator complex subunit 26
    Transcriptional coactivator CRSP70
    Gene namesi
    Name:MED26
    Synonyms:ARC70, CRSP7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:2376. MED26.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: UniProtKB
    2. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395623.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 600600Mediator of RNA polymerase II transcription subunit 26PRO_0000079360Add
    BLAST

    Proteomic databases

    MaxQBiO95402.
    PaxDbiO95402.
    PRIDEiO95402.

    PTM databases

    PhosphoSiteiO95402.

    Miscellaneous databases

    PMAP-CutDBO95402.

    Expressioni

    Gene expression databases

    ArrayExpressiO95402.
    BgeeiO95402.
    CleanExiHS_MED26.
    GenevestigatoriO95402.

    Organism-specific databases

    HPAiHPA044195.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EAF1Q96JC95EBI-394392,EBI-769261

    Protein-protein interaction databases

    BioGridi114831. 84 interactions.
    DIPiDIP-31464N.
    IntActiO95402. 90 interactions.
    STRINGi9606.ENSP00000263390.

    Structurei

    3D structure databases

    ProteinModelPortaliO95402.
    SMRiO95402. Positions 9-85.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 8778TFIIS N-terminalPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi228 – 32598Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 26 family.Curated
    Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG263228.
    HOGENOMiHOG000113539.
    HOVERGENiHBG051124.
    InParanoidiO95402.
    KOiK15169.
    OMAiHFMSEYL.
    OrthoDBiEOG7X0VGM.
    PhylomeDBiO95402.
    TreeFamiTF328436.

    Family and domain databases

    Gene3Di1.20.930.10. 1 hit.
    InterProiIPR003617. TFIIS/CRSP70_N_sub.
    IPR017923. TFIIS_N.
    [Graphical view]
    PfamiPF08711. Med26. 1 hit.
    [Graphical view]
    SMARTiSM00509. TFS2N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47676. SSF47676. 1 hit.
    PROSITEiPS51319. TFIIS_N. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O95402-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE    50
    TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQH EAALRGLAGA 100
    TGSANGGAHN CRPEVGAAGP PRSIHDLKSR NDLQRLPGQR LDRLGSRKRR 150
    GDQRDLGHPG PPPKVSKASH DPLVPNSSPL PTNGISGSPE SFASSLDGSG 200
    HAGPEGSRLE RDENDKHSGK IPVNAVRPHT SSPGLGKPPG PCLQPKASVL 250
    QQLDRVDETP GPPHPKGPPR CSFSPRNSRH EGSFARQQSL YAPKGSVPSP 300
    SPRPQALDAT QVPSPLPLAQ PSTPPVRRLE LLPSAESPVC WLEQPESHQR 350
    LAGPGCKAGL SPAEPLLSRA GFSPDSSKAD SDAASSGGSD SKKKKRYRPR 400
    DYTVNLDGQV AEAGVKPVRL KERKLTFDPM TRQIKPLTQK EPVRADSPVH 450
    MEQQSRTELD KQEAKASLQS PFEQTNWKEL SRNEIIQSYL SRQSSLLSSS 500
    GAQTPGAHHF MSEYLKQEES TRQGARQLHV LVPQSPPTDL PGLTREVTQD 550
    DLDRIQASQW PGVNGCQDTQ GNWYDWTQCI SLDPHGDDGR LNILPYVCLD 600
    Length:600
    Mass (Da):65,446
    Last modified:October 2, 2007 - v2
    Checksum:i4BEA264A3EB5A407
    GO
    Isoform 2 (identifier: O95402-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         76-92: LLRSWQKLIEPAHQHEA → LPGWQWACRPRGQPPGA
         93-600: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:92
    Mass (Da):10,359
    Checksum:i6837BD299DE56B5A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1561L → F in AAD12722. (PubMed:9989412)Curated
    Sequence conflicti241 – 2411P → A in AAI27216. (PubMed:15489334)Curated
    Sequence conflicti369 – 3691R → L in AAI10431. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei76 – 9217LLRSW…HQHEA → LPGWQWACRPRGQPPGA in isoform 2. 1 PublicationVSP_028151Add
    BLAST
    Alternative sequencei93 – 600508Missing in isoform 2. 1 PublicationVSP_028152Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104253 mRNA. Translation: AAD12722.1.
    BC110430 mRNA. Translation: AAI10431.1.
    BC127215 mRNA. Translation: AAI27216.1.
    CCDSiCCDS12347.1. [O95402-1]
    RefSeqiNP_004822.2. NM_004831.3. [O95402-1]
    UniGeneiHs.715040.

    Genome annotation databases

    EnsembliENST00000263390; ENSP00000263390; ENSG00000105085. [O95402-1]
    GeneIDi9441.
    KEGGihsa:9441.
    UCSCiuc002nen.1. human. [O95402-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF104253 mRNA. Translation: AAD12722.1 .
    BC110430 mRNA. Translation: AAI10431.1 .
    BC127215 mRNA. Translation: AAI27216.1 .
    CCDSi CCDS12347.1. [O95402-1 ]
    RefSeqi NP_004822.2. NM_004831.3. [O95402-1 ]
    UniGenei Hs.715040.

    3D structure databases

    ProteinModelPortali O95402.
    SMRi O95402. Positions 9-85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114831. 84 interactions.
    DIPi DIP-31464N.
    IntActi O95402. 90 interactions.
    STRINGi 9606.ENSP00000263390.

    PTM databases

    PhosphoSitei O95402.

    Proteomic databases

    MaxQBi O95402.
    PaxDbi O95402.
    PRIDEi O95402.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000263390 ; ENSP00000263390 ; ENSG00000105085 . [O95402-1 ]
    GeneIDi 9441.
    KEGGi hsa:9441.
    UCSCi uc002nen.1. human. [O95402-1 ]

    Organism-specific databases

    CTDi 9441.
    GeneCardsi GC19M016685.
    H-InvDB HIX0202876.
    HGNCi HGNC:2376. MED26.
    HPAi HPA044195.
    MIMi 605043. gene.
    neXtProti NX_O95402.
    PharmGKBi PA162395623.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG263228.
    HOGENOMi HOG000113539.
    HOVERGENi HBG051124.
    InParanoidi O95402.
    KOi K15169.
    OMAi HFMSEYL.
    OrthoDBi EOG7X0VGM.
    PhylomeDBi O95402.
    TreeFami TF328436.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    ChiTaRSi MED26. human.
    GeneWikii MED26.
    GenomeRNAii 9441.
    NextBioi 35354.
    PMAP-CutDB O95402.
    PROi O95402.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95402.
    Bgeei O95402.
    CleanExi HS_MED26.
    Genevestigatori O95402.

    Family and domain databases

    Gene3Di 1.20.930.10. 1 hit.
    InterProi IPR003617. TFIIS/CRSP70_N_sub.
    IPR017923. TFIIS_N.
    [Graphical view ]
    Pfami PF08711. Med26. 1 hit.
    [Graphical view ]
    SMARTi SM00509. TFS2N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47676. SSF47676. 1 hit.
    PROSITEi PS51319. TFIIS_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1."
      Ryu S., Zhou S., Ladurner A.G., Tjian R.
      Nature 397:446-450(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Uterus.
    3. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."
      Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
      Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN ARC COMPLEX, PROTEIN SEQUENCE OF 294-305 AND 478-489.
    4. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    5. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1 AND MED10, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED26_HUMAN
    AccessioniPrimary (citable) accession number: O95402
    Secondary accession number(s): A1A4S3, Q0VGB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3