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O95402

- MED26_HUMAN

UniProt

O95402 - MED26_HUMAN

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Protein
Mediator of RNA polymerase II transcription subunit 26
Gene
MED26, ARC70, CRSP7
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. RNA polymerase II transcription cofactor activity Source: UniProtKB
  3. protein binding Source: IntAct
  4. transcription coactivator activity Source: ProtInc
Complete GO annotation...

GO - Biological processi

  1. gene expression Source: Reactome
  2. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  3. transcription initiation from RNA polymerase II promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 26
Alternative name(s):
Activator-recruited cofactor 70 kDa component
Short name:
ARC70
Cofactor required for Sp1 transcriptional activation subunit 7
Short name:
CRSP complex subunit 7
Mediator complex subunit 26
Transcriptional coactivator CRSP70
Gene namesi
Name:MED26
Synonyms:ARC70, CRSP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:2376. MED26.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. mediator complex Source: UniProtKB
  2. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395623.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Mediator of RNA polymerase II transcription subunit 26
PRO_0000079360Add
BLAST

Proteomic databases

MaxQBiO95402.
PaxDbiO95402.
PRIDEiO95402.

PTM databases

PhosphoSiteiO95402.

Miscellaneous databases

PMAP-CutDBO95402.

Expressioni

Gene expression databases

ArrayExpressiO95402.
BgeeiO95402.
CleanExiHS_MED26.
GenevestigatoriO95402.

Organism-specific databases

HPAiHPA044195.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EAF1Q96JC95EBI-394392,EBI-769261

Protein-protein interaction databases

BioGridi114831. 84 interactions.
DIPiDIP-31464N.
IntActiO95402. 90 interactions.
STRINGi9606.ENSP00000263390.

Structurei

3D structure databases

ProteinModelPortaliO95402.
SMRiO95402. Positions 9-85.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 8778TFIIS N-terminal
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi228 – 32598Pro-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG263228.
HOGENOMiHOG000113539.
HOVERGENiHBG051124.
InParanoidiO95402.
KOiK15169.
OMAiHFMSEYL.
OrthoDBiEOG7X0VGM.
PhylomeDBiO95402.
TreeFamiTF328436.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
[Graphical view]
SMARTiSM00509. TFS2N. 1 hit.
[Graphical view]
SUPFAMiSSF47676. SSF47676. 1 hit.
PROSITEiPS51319. TFIIS_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O95402-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE    50
TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQH EAALRGLAGA 100
TGSANGGAHN CRPEVGAAGP PRSIHDLKSR NDLQRLPGQR LDRLGSRKRR 150
GDQRDLGHPG PPPKVSKASH DPLVPNSSPL PTNGISGSPE SFASSLDGSG 200
HAGPEGSRLE RDENDKHSGK IPVNAVRPHT SSPGLGKPPG PCLQPKASVL 250
QQLDRVDETP GPPHPKGPPR CSFSPRNSRH EGSFARQQSL YAPKGSVPSP 300
SPRPQALDAT QVPSPLPLAQ PSTPPVRRLE LLPSAESPVC WLEQPESHQR 350
LAGPGCKAGL SPAEPLLSRA GFSPDSSKAD SDAASSGGSD SKKKKRYRPR 400
DYTVNLDGQV AEAGVKPVRL KERKLTFDPM TRQIKPLTQK EPVRADSPVH 450
MEQQSRTELD KQEAKASLQS PFEQTNWKEL SRNEIIQSYL SRQSSLLSSS 500
GAQTPGAHHF MSEYLKQEES TRQGARQLHV LVPQSPPTDL PGLTREVTQD 550
DLDRIQASQW PGVNGCQDTQ GNWYDWTQCI SLDPHGDDGR LNILPYVCLD 600
Length:600
Mass (Da):65,446
Last modified:October 2, 2007 - v2
Checksum:i4BEA264A3EB5A407
GO
Isoform 2 (identifier: O95402-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-92: LLRSWQKLIEPAHQHEA → LPGWQWACRPRGQPPGA
     93-600: Missing.

Note: No experimental confirmation available.

Show »
Length:92
Mass (Da):10,359
Checksum:i6837BD299DE56B5A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei76 – 9217LLRSW…HQHEA → LPGWQWACRPRGQPPGA in isoform 2.
VSP_028151Add
BLAST
Alternative sequencei93 – 600508Missing in isoform 2.
VSP_028152Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561L → F in AAD12722. 1 Publication
Sequence conflicti241 – 2411P → A in AAI27216. 1 Publication
Sequence conflicti369 – 3691R → L in AAI10431. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF104253 mRNA. Translation: AAD12722.1.
BC110430 mRNA. Translation: AAI10431.1.
BC127215 mRNA. Translation: AAI27216.1.
CCDSiCCDS12347.1. [O95402-1]
RefSeqiNP_004822.2. NM_004831.3. [O95402-1]
UniGeneiHs.715040.

Genome annotation databases

EnsembliENST00000263390; ENSP00000263390; ENSG00000105085. [O95402-1]
GeneIDi9441.
KEGGihsa:9441.
UCSCiuc002nen.1. human. [O95402-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF104253 mRNA. Translation: AAD12722.1 .
BC110430 mRNA. Translation: AAI10431.1 .
BC127215 mRNA. Translation: AAI27216.1 .
CCDSi CCDS12347.1. [O95402-1 ]
RefSeqi NP_004822.2. NM_004831.3. [O95402-1 ]
UniGenei Hs.715040.

3D structure databases

ProteinModelPortali O95402.
SMRi O95402. Positions 9-85.
ModBasei Search...

Protein-protein interaction databases

BioGridi 114831. 84 interactions.
DIPi DIP-31464N.
IntActi O95402. 90 interactions.
STRINGi 9606.ENSP00000263390.

PTM databases

PhosphoSitei O95402.

Proteomic databases

MaxQBi O95402.
PaxDbi O95402.
PRIDEi O95402.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263390 ; ENSP00000263390 ; ENSG00000105085 . [O95402-1 ]
GeneIDi 9441.
KEGGi hsa:9441.
UCSCi uc002nen.1. human. [O95402-1 ]

Organism-specific databases

CTDi 9441.
GeneCardsi GC19M016685.
H-InvDB HIX0202876.
HGNCi HGNC:2376. MED26.
HPAi HPA044195.
MIMi 605043. gene.
neXtProti NX_O95402.
PharmGKBi PA162395623.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG263228.
HOGENOMi HOG000113539.
HOVERGENi HBG051124.
InParanoidi O95402.
KOi K15169.
OMAi HFMSEYL.
OrthoDBi EOG7X0VGM.
PhylomeDBi O95402.
TreeFami TF328436.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_12627. Generic Transcription Pathway.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSi MED26. human.
GeneWikii MED26.
GenomeRNAii 9441.
NextBioi 35354.
PMAP-CutDB O95402.
PROi O95402.
SOURCEi Search...

Gene expression databases

ArrayExpressi O95402.
Bgeei O95402.
CleanExi HS_MED26.
Genevestigatori O95402.

Family and domain databases

Gene3Di 1.20.930.10. 1 hit.
InterProi IPR003617. TFIIS/CRSP70_N_sub.
IPR017923. TFIIS_N.
[Graphical view ]
Pfami PF08711. Med26. 1 hit.
[Graphical view ]
SMARTi SM00509. TFS2N. 1 hit.
[Graphical view ]
SUPFAMi SSF47676. SSF47676. 1 hit.
PROSITEi PS51319. TFIIS_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1."
    Ryu S., Zhou S., Ladurner A.G., Tjian R.
    Nature 397:446-450(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Uterus.
  3. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."
    Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
    Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN ARC COMPLEX, PROTEIN SEQUENCE OF 294-305 AND 478-489.
  4. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  5. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED1 AND MED10, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED26_HUMAN
AccessioniPrimary (citable) accession number: O95402
Secondary accession number(s): A1A4S3, Q0VGB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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