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Protein

Mediator of RNA polymerase II transcription subunit 26

Gene

MED26

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  • RNA polymerase II transcription cofactor activity Source: UniProtKB
  • transcription coactivator activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 26
Alternative name(s):
Activator-recruited cofactor 70 kDa component
Short name:
ARC70
Cofactor required for Sp1 transcriptional activation subunit 7
Short name:
CRSP complex subunit 7
Mediator complex subunit 26
Transcriptional coactivator CRSP70
Gene namesi
Name:MED26
Synonyms:ARC70, CRSP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105085.10
HGNCiHGNC:2376 MED26
MIMi605043 gene
neXtProtiNX_O95402

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000105085
PharmGKBiPA162395623

Polymorphism and mutation databases

BioMutaiMED26

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793601 – 600Mediator of RNA polymerase II transcription subunit 26Add BLAST600

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei447PhosphoserineCombined sources1
Modified residuei470PhosphoserineCombined sources1
Modified residuei535PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO95402
MaxQBiO95402
PaxDbiO95402
PeptideAtlasiO95402
PRIDEiO95402
TopDownProteomicsiO95402-1 [O95402-1]

PTM databases

iPTMnetiO95402
PhosphoSitePlusiO95402

Miscellaneous databases

PMAP-CutDBiO95402

Expressioni

Gene expression databases

BgeeiENSG00000105085
CleanExiHS_MED26
ExpressionAtlasiO95402 baseline and differential
GenevisibleiO95402 HS

Organism-specific databases

HPAiHPA071835

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CEBPB (when not methylated)(PubMed:20111005).4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114831, 87 interactors
CORUMiO95402
DIPiDIP-31464N
IntActiO95402, 95 interactors
STRINGi9606.ENSP00000263390

Structurei

Secondary structure

1600
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 18Combined sources11
Helixi28 – 40Combined sources13
Helixi45 – 50Combined sources6
Turni51 – 53Combined sources3
Helixi54 – 63Combined sources10
Helixi67 – 81Combined sources15
Turni82 – 84Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ODDNMR-A1-92[»]
ProteinModelPortaliO95402
SMRiO95402
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 87TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi228 – 325Pro-richAdd BLAST98

Sequence similaritiesi

Belongs to the Mediator complex subunit 26 family.Curated

Phylogenomic databases

eggNOGiKOG1105 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00390000000259
HOGENOMiHOG000113539
HOVERGENiHBG051124
InParanoidiO95402
KOiK15169
OMAiHFMSEYL
OrthoDBiEOG091G0H00
PhylomeDBiO95402
TreeFamiTF328436

Family and domain databases

Gene3Di1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR031416 Med26_C
IPR031417 Med26_Mid
IPR003617 TFIIS/CRSP70_N_sub
IPR035441 TFIIS/LEDGF_dom_sf
IPR017923 TFIIS_N
PfamiView protein in Pfam
PF08711 Med26, 1 hit
PF15693 Med26_C, 1 hit
PF15694 Med26_M, 1 hit
SMARTiView protein in SMART
SM00509 TFS2N, 1 hit
SUPFAMiSSF47676 SSF47676, 1 hit
PROSITEiView protein in PROSITE
PS51319 TFIIS_N, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95402-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAAPASPQQ IRDRLLQAID PQSNIRNMVA VLEVISSLEK YPITKEALEE
60 70 80 90 100
TRLGKLINDV RKKTKNEELA KRAKKLLRSW QKLIEPAHQH EAALRGLAGA
110 120 130 140 150
TGSANGGAHN CRPEVGAAGP PRSIHDLKSR NDLQRLPGQR LDRLGSRKRR
160 170 180 190 200
GDQRDLGHPG PPPKVSKASH DPLVPNSSPL PTNGISGSPE SFASSLDGSG
210 220 230 240 250
HAGPEGSRLE RDENDKHSGK IPVNAVRPHT SSPGLGKPPG PCLQPKASVL
260 270 280 290 300
QQLDRVDETP GPPHPKGPPR CSFSPRNSRH EGSFARQQSL YAPKGSVPSP
310 320 330 340 350
SPRPQALDAT QVPSPLPLAQ PSTPPVRRLE LLPSAESPVC WLEQPESHQR
360 370 380 390 400
LAGPGCKAGL SPAEPLLSRA GFSPDSSKAD SDAASSGGSD SKKKKRYRPR
410 420 430 440 450
DYTVNLDGQV AEAGVKPVRL KERKLTFDPM TRQIKPLTQK EPVRADSPVH
460 470 480 490 500
MEQQSRTELD KQEAKASLQS PFEQTNWKEL SRNEIIQSYL SRQSSLLSSS
510 520 530 540 550
GAQTPGAHHF MSEYLKQEES TRQGARQLHV LVPQSPPTDL PGLTREVTQD
560 570 580 590 600
DLDRIQASQW PGVNGCQDTQ GNWYDWTQCI SLDPHGDDGR LNILPYVCLD
Length:600
Mass (Da):65,446
Last modified:October 2, 2007 - v2
Checksum:i4BEA264A3EB5A407
GO
Isoform 2 (identifier: O95402-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     76-92: LLRSWQKLIEPAHQHEA → LPGWQWACRPRGQPPGA
     93-600: Missing.

Note: No experimental confirmation available.
Show »
Length:92
Mass (Da):10,359
Checksum:i6837BD299DE56B5A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → F in AAD12722 (PubMed:9989412).Curated1
Sequence conflicti241P → A in AAI27216 (PubMed:15489334).Curated1
Sequence conflicti369R → L in AAI10431 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02815176 – 92LLRSW…HQHEA → LPGWQWACRPRGQPPGA in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_02815293 – 600Missing in isoform 2. 1 PublicationAdd BLAST508

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104253 mRNA Translation: AAD12722.1
BC110430 mRNA Translation: AAI10431.1
BC127215 mRNA Translation: AAI27216.1
CCDSiCCDS12347.1 [O95402-1]
RefSeqiNP_004822.2, NM_004831.3 [O95402-1]
UniGeneiHs.715040

Genome annotation databases

EnsembliENST00000263390; ENSP00000263390; ENSG00000105085 [O95402-1]
ENST00000611692; ENSP00000484490; ENSG00000105085 [O95402-2]
GeneIDi9441
KEGGihsa:9441
UCSCiuc002nen.2 human [O95402-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED26_HUMAN
AccessioniPrimary (citable) accession number: O95402
Secondary accession number(s): A1A4S3, Q0VGB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 2, 2007
Last modified: March 28, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health