##gff-version 3
O95396	UniProtKB	Chain	1	460	.	.	.	ID=PRO_0000120583;Note=Adenylyltransferase and sulfurtransferase MOCS3	
O95396	UniProtKB	Domain	347	458	.	.	.	Note=Rhodanese;Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Region	158	238	.	.	.	Note=Interaction with NFS1;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:23593335;Dbxref=PMID:23593335	
O95396	UniProtKB	Active site	239	239	.	.	.	Note=Glycyl thioester intermediate%3B for adenylyltransferase activity;Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Active site	412	412	.	.	.	Note=Cysteine persulfide intermediate%3B for sulfurtransferase activity;Ontology_term=ECO:0000255,ECO:0000269,ECO:0000269;evidence=ECO:0000255|HAMAP-Rule:MF_03049,ECO:0000269|PubMed:15910006,ECO:0000269|PubMed:18650437;Dbxref=PMID:15910006,PMID:18650437	
O95396	UniProtKB	Binding site	92	92	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	113	113	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	120	124	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	137	137	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	181	182	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	222	222	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	225	225	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	297	297	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Binding site	300	300	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|HAMAP-Rule:MF_03049	
O95396	UniProtKB	Modified residue	412	412	.	.	.	Note=Cysteine persulfide;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15910006,ECO:0000269|PubMed:18650437;Dbxref=PMID:15910006,PMID:18650437	
O95396	UniProtKB	Disulfide bond	316	324	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15910006;Dbxref=PMID:15910006	
O95396	UniProtKB	Natural variant	429	429	.	.	.	ID=VAR_049349;Note=S->A;Dbxref=dbSNP:rs7269297	
O95396	UniProtKB	Mutagenesis	239	239	.	.	.	Note=Impairs sulfurtransferase activity. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15073332;Dbxref=PMID:15073332	
O95396	UniProtKB	Mutagenesis	316	316	.	.	.	Note=Does not affect sulfurtransferase activity. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15073332;Dbxref=PMID:15073332	
O95396	UniProtKB	Mutagenesis	324	324	.	.	.	Note=Does not affect sulfurtransferase activity. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15073332;Dbxref=PMID:15073332	
O95396	UniProtKB	Mutagenesis	365	365	.	.	.	Note=Does not affect sulfurtransferase activity. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15073332;Dbxref=PMID:15073332	
O95396	UniProtKB	Mutagenesis	412	412	.	.	.	Note=Abolishes sulfurtransferase activity. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15073332;Dbxref=PMID:15073332	
O95396	UniProtKB	Mutagenesis	413	413	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	414	414	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. L->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	415	415	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	416	416	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. N->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	417	417	.	.	.	Note=Results in 470-fold increased activity. D->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	417	417	.	.	.	Note=Results in 90-fold increased activity. D->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	458	458	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. P->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Mutagenesis	460	460	.	.	.	Note=Does not affect sulfurtransferase specificity and activity. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17459099;Dbxref=PMID:17459099	
O95396	UniProtKB	Helix	338	347	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	352	355	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Helix	359	364	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	370	372	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Helix	375	379	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Helix	383	397	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	406	411	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	413	416	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Helix	417	430	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	433	435	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Beta strand	437	442	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V	
O95396	UniProtKB	Helix	445	452	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I2V