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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O3-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O3-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].1 Publication
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-(beta-D-GlcNAc-(1->6))-beta-D-Gal-(1->4)-beta-D-GlcNAc-R.1 Publication
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-(N-acetyl-beta-D-glucosaminyl-(1->3))-N-acetyl-alpha-D-galactosaminyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • immunoglobulin production in mucosal tissue Source: Ensembl
  • intestinal absorption Source: Ensembl
  • kidney morphogenesis Source: Ensembl
  • O-glycan processing Source: Reactome
  • protein O-linked glycosylation Source: ProtInc
  • tissue morphogenesis Source: Ensembl

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS06698-MONOMER
BRENDAi2.4.1.102 2681
ReactomeiR-HSA-913709 O-linked glycosylation of mucins
SignaLinkiO95395
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT14 Glycosyltransferase Family 14

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.1021 Publication, EC:2.4.1.1481 Publication, EC:2.4.1.1501 Publication)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Short name:
hC2GnT-M
Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
Short name:
C2/4GnT
Gene namesi
Name:GCNT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000140297.12
HGNCiHGNC:4205 GCNT3
MIMi606836 gene
neXtProtiNX_O95395

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini27 – 438LumenalSequence analysisAdd BLAST412

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9245
OpenTargetsiENSG00000140297
PharmGKBiPA28620

Polymorphism and mutation databases

BioMutaiGCNT3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885451 – 438Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3Add BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 227By similarity
Disulfide bondi161 ↔ 382By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi289N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi391 ↔ 423By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO95395
PaxDbiO95395
PeptideAtlasiO95395
PRIDEiO95395

PTM databases

iPTMnetiO95395
PhosphoSitePlusiO95395

Expressioni

Tissue specificityi

Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.2 Publications

Inductioni

By all-trans retinoic acid (ATRA), TNF and IL13/interleukin-13. Strongly down-regulated in colorectal cancer.3 Publications

Gene expression databases

BgeeiENSG00000140297
CleanExiHS_GCNT3
ExpressionAtlasiO95395 baseline and differential
GenevisibleiO95395 HS

Organism-specific databases

HPAiHPA011154

Interactioni

Protein-protein interaction databases

BioGridi114671, 12 interactors
IntActiO95395, 4 interactors
STRINGi9606.ENSP00000379377

Structurei

3D structure databases

ProteinModelPortaliO95395
SMRiO95395
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799 Eukaryota
ENOG410XQ7M LUCA
GeneTreeiENSGT00760000119183
HOGENOMiHOG000293251
HOVERGENiHBG051711
InParanoidiO95395
KOiK09662
OMAiHLLANKF
OrthoDBiEOG091G0HLZ
PhylomeDBiO95395
TreeFamiTF315534

Family and domain databases

InterProiView protein in InterPro
IPR003406 Glyco_trans_14
PfamiView protein in Pfam
PF02485 Branch, 1 hit

Sequencei

Sequence statusi: Complete.

O95395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQWKRLCQL HYLWALGCYM LLATVALKLS FRLKCDSDHL GLESRESQSQ
60 70 80 90 100
YCRNILYNFL KLPAKRSINC SGVTRGDQEA VLQAILNNLE VKKKREPFTD
110 120 130 140 150
THYLSLTRDC EHFKAERKFI QFPLSKEEVE FPIAYSMVIH EKIENFERLL
160 170 180 190 200
RAVYAPQNIY CVHVDEKSPE TFKEAVKAII SCFPNVFIAS KLVRVVYASW
210 220 230 240 250
SRVQADLNCM EDLLQSSVPW KYFLNTCGTD FPIKSNAEMV QALKMLNGRN
260 270 280 290 300
SMESEVPPKH KETRWKYHFE VVRDTLHLTN KKKDPPPYNL TMFTGNAYIV
310 320 330 340 350
ASRDFVQHVL KNPKSQQLIE WVKDTYSPDE HLWATLQRAR WMPGSVPNHP
360 370 380 390 400
KYDISDMTSI ARLVKWQGHE GDIDKGAPYA PCSGIHQRAI CVYGAGDLNW
410 420 430
MLQNHHLLAN KFDPKVDDNA LQCLEEYLRY KAIYGTEL
Length:438
Mass (Da):50,864
Last modified:May 1, 1999 - v1
Checksum:i1FF0A7B451C88407
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102542 mRNA Translation: AAD10824.1
AF038650 mRNA Translation: AAD21525.1
EF152283 mRNA Translation: ABM21534.1
BC017032 mRNA Translation: AAH17032.1
CCDSiCCDS10172.1
RefSeqiNP_004742.1, NM_004751.2
UniGeneiHs.194710

Genome annotation databases

EnsembliENST00000396065; ENSP00000379377; ENSG00000140297
ENST00000560585; ENSP00000452741; ENSG00000140297
GeneIDi9245
KEGGihsa:9245
UCSCiuc002agd.4 human

Similar proteinsi

Entry informationi

Entry nameiGCNT3_HUMAN
AccessioniPrimary (citable) accession number: O95395
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health