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O95395

- GCNT3_HUMAN

UniProt

O95395 - GCNT3_HUMAN

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.1 Publication

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.1 Publication
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
    2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: ProtInc

    GO - Biological processi

    1. carbohydrate metabolic process Source: ProtInc
    2. cellular protein metabolic process Source: Reactome
    3. immunoglobulin production in mucosal tissue Source: Ensembl
    4. intestinal absorption Source: Ensembl
    5. kidney morphogenesis Source: Ensembl
    6. O-glycan processing Source: Reactome
    7. post-translational protein modification Source: Reactome
    8. protein O-linked glycosylation Source: ProtInc
    9. tissue morphogenesis Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_115606. O-linked glycosylation of mucins.
    SignaLinkiO95395.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Short name:
    C2GnT-M
    Short name:
    hC2GnT-M
    Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
    Short name:
    C2/4GnT
    Gene namesi
    Name:GCNT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:4205. GCNT3.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. Golgi membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: ProtInc

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28620.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 438438Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288545Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 227By similarity
    Disulfide bondi161 ↔ 382By similarity
    Disulfide bondi182 ↔ 209By similarity
    Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi391 ↔ 423By similarity

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO95395.
    PaxDbiO95395.
    PRIDEiO95395.

    PTM databases

    PhosphoSiteiO95395.

    Expressioni

    Tissue specificityi

    Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.2 Publications

    Inductioni

    By all-trans retinoic acid (ATRA), TNF and IL13/interleukin-13. Strongly down-regulated in colorectal cancer.3 Publications

    Gene expression databases

    ArrayExpressiO95395.
    BgeeiO95395.
    CleanExiHS_GCNT3.
    GenevestigatoriO95395.

    Organism-specific databases

    HPAiHPA011154.

    Interactioni

    Protein-protein interaction databases

    BioGridi114671. 4 interactions.
    IntActiO95395. 4 interactions.
    STRINGi9606.ENSP00000267857.

    Structurei

    3D structure databases

    ProteinModelPortaliO95395.
    SMRiO95395. Positions 67-433.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini27 – 438412LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2620Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253565.
    HOGENOMiHOG000293251.
    HOVERGENiHBG051711.
    InParanoidiO95395.
    KOiK09662.
    OMAiPCSGIHQ.
    PhylomeDBiO95395.
    TreeFamiTF315534.

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O95395-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQWKRLCQL HYLWALGCYM LLATVALKLS FRLKCDSDHL GLESRESQSQ    50
    YCRNILYNFL KLPAKRSINC SGVTRGDQEA VLQAILNNLE VKKKREPFTD 100
    THYLSLTRDC EHFKAERKFI QFPLSKEEVE FPIAYSMVIH EKIENFERLL 150
    RAVYAPQNIY CVHVDEKSPE TFKEAVKAII SCFPNVFIAS KLVRVVYASW 200
    SRVQADLNCM EDLLQSSVPW KYFLNTCGTD FPIKSNAEMV QALKMLNGRN 250
    SMESEVPPKH KETRWKYHFE VVRDTLHLTN KKKDPPPYNL TMFTGNAYIV 300
    ASRDFVQHVL KNPKSQQLIE WVKDTYSPDE HLWATLQRAR WMPGSVPNHP 350
    KYDISDMTSI ARLVKWQGHE GDIDKGAPYA PCSGIHQRAI CVYGAGDLNW 400
    MLQNHHLLAN KFDPKVDDNA LQCLEEYLRY KAIYGTEL 438
    Length:438
    Mass (Da):50,864
    Last modified:May 1, 1999 - v1
    Checksum:i1FF0A7B451C88407
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF102542 mRNA. Translation: AAD10824.1.
    AF038650 mRNA. Translation: AAD21525.1.
    EF152283 mRNA. Translation: ABM21534.1.
    BC017032 mRNA. Translation: AAH17032.1.
    CCDSiCCDS10172.1.
    RefSeqiNP_004742.1. NM_004751.2.
    UniGeneiHs.194710.

    Genome annotation databases

    EnsembliENST00000396065; ENSP00000379377; ENSG00000140297.
    ENST00000560585; ENSP00000452741; ENSG00000140297.
    GeneIDi9245.
    KEGGihsa:9245.
    UCSCiuc002agd.3. human.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology
    GGDB

    GlycoGene database

    Functional Glycomics Gateway - GTase

    Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF102542 mRNA. Translation: AAD10824.1 .
    AF038650 mRNA. Translation: AAD21525.1 .
    EF152283 mRNA. Translation: ABM21534.1 .
    BC017032 mRNA. Translation: AAH17032.1 .
    CCDSi CCDS10172.1.
    RefSeqi NP_004742.1. NM_004751.2.
    UniGenei Hs.194710.

    3D structure databases

    ProteinModelPortali O95395.
    SMRi O95395. Positions 67-433.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114671. 4 interactions.
    IntActi O95395. 4 interactions.
    STRINGi 9606.ENSP00000267857.

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    PTM databases

    PhosphoSitei O95395.

    Proteomic databases

    MaxQBi O95395.
    PaxDbi O95395.
    PRIDEi O95395.

    Protocols and materials databases

    DNASUi 9245.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396065 ; ENSP00000379377 ; ENSG00000140297 .
    ENST00000560585 ; ENSP00000452741 ; ENSG00000140297 .
    GeneIDi 9245.
    KEGGi hsa:9245.
    UCSCi uc002agd.3. human.

    Organism-specific databases

    CTDi 9245.
    GeneCardsi GC15P059903.
    HGNCi HGNC:4205. GCNT3.
    HPAi HPA011154.
    MIMi 606836. gene.
    neXtProti NX_O95395.
    PharmGKBi PA28620.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG253565.
    HOGENOMi HOG000293251.
    HOVERGENi HBG051711.
    InParanoidi O95395.
    KOi K09662.
    OMAi PCSGIHQ.
    PhylomeDBi O95395.
    TreeFami TF315534.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_115606. O-linked glycosylation of mucins.
    SignaLinki O95395.

    Miscellaneous databases

    ChiTaRSi GCNT3. human.
    GenomeRNAii 9245.
    NextBioi 34657.
    PROi O95395.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O95395.
    Bgeei O95395.
    CleanExi HS_GCNT3.
    Genevestigatori O95395.

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches."
      Yeh J.-C., Ong E., Fukuda M.
      J. Biol. Chem. 274:3215-3221(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "Control of O-glycan branch formation. Molecular cloning of human cDNA encoding a novel beta1,6-N-acetylglucosaminyltransferase forming core 2 and core 4."
      Schwientek T., Nomoto M., Levery S.B., Merkx G., van Kessel A.G., Bennett E.P., Hollingsworth M.A., Clausen H.
      J. Biol. Chem. 274:4504-4512(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Mucin biosynthesis: identification of the cis-regulatory elements of human C2GnT-M gene."
      Tan S., Cheng P.-W.
      Am. J. Respir. Cell Mol. Biol. 36:737-745(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    5. "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line."
      Ishibashi Y., Inouye Y., Okano T., Taniguchi A.
      Glycoconj. J. 22:53-62(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "C2GnT-M is downregulated in colorectal cancer and its re-expression causes growth inhibition of colon cancer cells."
      Huang M.-C., Chen H.-Y., Huang H.-C., Huang J., Liang J.-T., Shen T.-L., Lin N.-Y., Ho C.-C., Cho I.-M., Hsu S.-M.
      Oncogene 25:3267-3276(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiGCNT3_HUMAN
    AccessioniPrimary (citable) accession number: O95395
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3