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O95395

- GCNT3_HUMAN

UniProt

O95395 - GCNT3_HUMAN

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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene
GCNT3
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.1 Publication
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
  2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: ProtInc

GO - Biological processi

  1. carbohydrate metabolic process Source: ProtInc
  2. cellular protein metabolic process Source: Reactome
  3. immunoglobulin production in mucosal tissue Source: Ensembl
  4. intestinal absorption Source: Ensembl
  5. kidney morphogenesis Source: Ensembl
  6. O-glycan processing Source: Reactome
  7. post-translational protein modification Source: Reactome
  8. protein O-linked glycosylation Source: ProtInc
  9. tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_115606. O-linked glycosylation of mucins.
SignaLinkiO95395.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Short name:
C2GnT-M
Short name:
hC2GnT-M
Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase
Short name:
C2/4GnT
Gene namesi
Name:GCNT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:4205. GCNT3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Cytoplasmic Reviewed prediction
Transmembranei7 – 2620Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini27 – 438412Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28620.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 227 By similarity
Disulfide bondi161 ↔ 382 By similarity
Disulfide bondi182 ↔ 209 By similarity
Glycosylationi289 – 2891N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi391 ↔ 423 By similarity

Post-translational modificationi

N-glycosylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO95395.
PaxDbiO95395.
PRIDEiO95395.

PTM databases

PhosphoSiteiO95395.

Expressioni

Tissue specificityi

Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.2 Publications

Inductioni

By all-trans retinoic acid (ATRA), TNF and IL13/interleukin-13. Strongly down-regulated in colorectal cancer.3 Publications

Gene expression databases

ArrayExpressiO95395.
BgeeiO95395.
CleanExiHS_GCNT3.
GenevestigatoriO95395.

Organism-specific databases

HPAiHPA011154.

Interactioni

Protein-protein interaction databases

BioGridi114671. 4 interactions.
IntActiO95395. 4 interactions.
STRINGi9606.ENSP00000267857.

Structurei

3D structure databases

ProteinModelPortaliO95395.
SMRiO95395. Positions 67-433.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiO95395.
KOiK09662.
OMAiPCSGIHQ.
PhylomeDBiO95395.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O95395-1 [UniParc]FASTAAdd to Basket

« Hide

MVQWKRLCQL HYLWALGCYM LLATVALKLS FRLKCDSDHL GLESRESQSQ    50
YCRNILYNFL KLPAKRSINC SGVTRGDQEA VLQAILNNLE VKKKREPFTD 100
THYLSLTRDC EHFKAERKFI QFPLSKEEVE FPIAYSMVIH EKIENFERLL 150
RAVYAPQNIY CVHVDEKSPE TFKEAVKAII SCFPNVFIAS KLVRVVYASW 200
SRVQADLNCM EDLLQSSVPW KYFLNTCGTD FPIKSNAEMV QALKMLNGRN 250
SMESEVPPKH KETRWKYHFE VVRDTLHLTN KKKDPPPYNL TMFTGNAYIV 300
ASRDFVQHVL KNPKSQQLIE WVKDTYSPDE HLWATLQRAR WMPGSVPNHP 350
KYDISDMTSI ARLVKWQGHE GDIDKGAPYA PCSGIHQRAI CVYGAGDLNW 400
MLQNHHLLAN KFDPKVDDNA LQCLEEYLRY KAIYGTEL 438
Length:438
Mass (Da):50,864
Last modified:May 1, 1999 - v1
Checksum:i1FF0A7B451C88407
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF102542 mRNA. Translation: AAD10824.1.
AF038650 mRNA. Translation: AAD21525.1.
EF152283 mRNA. Translation: ABM21534.1.
BC017032 mRNA. Translation: AAH17032.1.
CCDSiCCDS10172.1.
RefSeqiNP_004742.1. NM_004751.2.
UniGeneiHs.194710.

Genome annotation databases

EnsembliENST00000396065; ENSP00000379377; ENSG00000140297.
ENST00000560585; ENSP00000452741; ENSG00000140297.
GeneIDi9245.
KEGGihsa:9245.
UCSCiuc002agd.3. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF102542 mRNA. Translation: AAD10824.1 .
AF038650 mRNA. Translation: AAD21525.1 .
EF152283 mRNA. Translation: ABM21534.1 .
BC017032 mRNA. Translation: AAH17032.1 .
CCDSi CCDS10172.1.
RefSeqi NP_004742.1. NM_004751.2.
UniGenei Hs.194710.

3D structure databases

ProteinModelPortali O95395.
SMRi O95395. Positions 67-433.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114671. 4 interactions.
IntActi O95395. 4 interactions.
STRINGi 9606.ENSP00000267857.

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSitei O95395.

Proteomic databases

MaxQBi O95395.
PaxDbi O95395.
PRIDEi O95395.

Protocols and materials databases

DNASUi 9245.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000396065 ; ENSP00000379377 ; ENSG00000140297 .
ENST00000560585 ; ENSP00000452741 ; ENSG00000140297 .
GeneIDi 9245.
KEGGi hsa:9245.
UCSCi uc002agd.3. human.

Organism-specific databases

CTDi 9245.
GeneCardsi GC15P059903.
HGNCi HGNC:4205. GCNT3.
HPAi HPA011154.
MIMi 606836. gene.
neXtProti NX_O95395.
PharmGKBi PA28620.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG253565.
HOGENOMi HOG000293251.
HOVERGENi HBG051711.
InParanoidi O95395.
KOi K09662.
OMAi PCSGIHQ.
PhylomeDBi O95395.
TreeFami TF315534.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_115606. O-linked glycosylation of mucins.
SignaLinki O95395.

Miscellaneous databases

ChiTaRSi GCNT3. human.
GenomeRNAii 9245.
NextBioi 34657.
PROi O95395.
SOURCEi Search...

Gene expression databases

ArrayExpressi O95395.
Bgeei O95395.
CleanExi HS_GCNT3.
Genevestigatori O95395.

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a novel beta-1, 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches."
    Yeh J.-C., Ong E., Fukuda M.
    J. Biol. Chem. 274:3215-3221(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Control of O-glycan branch formation. Molecular cloning of human cDNA encoding a novel beta1,6-N-acetylglucosaminyltransferase forming core 2 and core 4."
    Schwientek T., Nomoto M., Levery S.B., Merkx G., van Kessel A.G., Bennett E.P., Hollingsworth M.A., Clausen H.
    J. Biol. Chem. 274:4504-4512(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Mucin biosynthesis: identification of the cis-regulatory elements of human C2GnT-M gene."
    Tan S., Cheng P.-W.
    Am. J. Respir. Cell Mol. Biol. 36:737-745(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  5. "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line."
    Ishibashi Y., Inouye Y., Okano T., Taniguchi A.
    Glycoconj. J. 22:53-62(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "C2GnT-M is downregulated in colorectal cancer and its re-expression causes growth inhibition of colon cancer cells."
    Huang M.-C., Chen H.-Y., Huang H.-C., Huang J., Liang J.-T., Shen T.-L., Lin N.-Y., Ho C.-C., Cho I.-M., Hsu S.-M.
    Oncogene 25:3267-3276(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiGCNT3_HUMAN
AccessioniPrimary (citable) accession number: O95395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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